Eastern Saudi Arabia Research Articles

The experimental infection with a field isolate of the infectious bronchitis virus from eastern Saudi Arabia resulted in seroconversion of the challenged birds with no apparent clinical diseases.

The infectious bronchitis virus (IBV) is still one of the major respiratory viral pathogens of chickens. The IBV infection resulted in a wide range of clinical syndromes in the affected chickens, including respiratory, renal, gonads affections as well as generalized infections. Despite the intensive application of various commercial vaccines against the virus, many outbreaks are still reported in chickens worldwide. Several studies reported the circulation of several strains and genotypes of the IBV in eastern Saudi Arabia. The main goal of the current study was to isolate some of the circulating strains of IBV and assess its ability to reproduce the IBV infections in the challenge birds. Another objective was to monitor the immune status of the infected chickens during the course of this study. To achieve these goals, we used some filed IBV isolates retrieved from an outbreak in a broiler chicken farm in eastern Saudi Arabia in 2014. A total of 220-day-old chickens (110 Ross and 110 native Saudi breed chickens), twenty birds per each group, were used in this study. The chickens in some groups received some IBV vaccines on day one of the experiment, and some are boosted on day 19. All birds were challenged on day 28 of the experiment. Our results showed mild IBV signs in the non-vaccinated control group of chickens; however, the vaccinated chickens did not show any signs of IBV infections. Meanwhile, both the vaccinated and the none- vaccinated birds seroconverted to the IBV as shown by the ELISA results. In conclusion, the response of the IBV infected birds is mainly driven by the vaccination plans they received as a prime-boost regime. Further studies are required for a better understanding of the dynamics of IBV infection in native Saudi chickens.

Risk Factors of Early Childhood Caries Among Preschool Children in Eastern Saudi Arabia.

This study aimed to determine the risk factors of early childhood caries (ECC) among preschool children from eastern Saudi Arabia. In this cross-sectional study, 241 carers and their children from 10 daycares were recruited and asked to complete a questionnaire aimed at assessing their sociodemographic profile and oral health-related behaviors. This was followed by a dental examination of the children by two calibrated dentists. Caries status was recorded using the dmft index. Chi-square and logistic regression tests were used to analyze the data (p < 0.05). The mean dmft of the children was 4.39 (SD ± 4.25). According to logistic regression analysis, children who were bottle-fed between 7 and 12 months (Adjusted Odds Ratio (AOR): 0.110) or breastfed between 13 and 18 months (AOR: 0.028) were less likely to have ECC than those with a prolonged feeding duration (>18 months). Also, those with smoking carers (AOR: 0.176) were less likely to have ECC than those with nonsmoking carers. On the other hand, children who had working carers, mainly mothers, in the education sector (AOR: 11.105), were more likely to have ECC (p < 0.05). The risk factors associated with the presence of ECC among preschool children in eastern Saudi Arabia include the mother's occupation and the carer's smoking status, as well as feeding practices (particularly bottle feeding). These factors can be modified by encouraging carers to completely wean their children (particularly from the bottle) by 18 months of age and advising working mothers to perform tooth brushing for their children in the morning before they go to work and in the evening before the children go to bed.

Use of social networking in the Middle East: student perspectives in higher education

This study aims to determine the benefits, risks, awareness, cultural factors, and sustainability, allied to social networking (SN) use in the higher education (HE) sector in Middle Eastern countries, namely Jordan, Saudi Arabia, and Turkey. Using an online survey, 1180 complete responses were collected and analyzed using the statistical confirmatory factor analysis method. The use of SN in the Middle Eastern HE sector has the capacity to promote and motivate students to acquire professional and personal skills for their studies and future workplace; however, the use of SN by tertiary students is also associated with several risks: isolation, depression, privacy, and security. Furthermore, culture is influenced by using SN use, since some countries shifted from one dimension to another based on Hofstede's cultural framework. The study new findings are based on a sample at a specific point in time within a culture. The study findings encourage academics to include SN in unit activities and assessments to reap the benefits of SN, while taking steps to mitigate any risks that SN poses to students. Although other studies in the Middle East examined the use of Learning Management System and Facebook in, HE as a means of engaging students in discussions and communications, however, this study contributes a better understanding of the benefits and risks, awareness, culture, and sustainability, associated with the use of SN in the HE sector in the Middle East. Finally, the paper concludes with an acknowledgment of the study limitations and suggestions for future research.

Diversity and Risk Factors Associated with Multidrug and Methicillin-Resistant Staphylococci Isolated from Cats Admitted to a Veterinary Clinic in Eastern Province, Saudi Arabia.

Understanding the distribution, antimicrobial resistance (AMR), and risk factors associated with multidrug-resistant (MDR) and methicillin-resistant staphylococci (MRS) isolated from cats admitted to veterinary clinics may decrease the risk of MDR and MRS transmission to humans and other cats. As such, the objectives of this study were to investigate the diversity in Staphylococcus spp. recovered from different anatomical locations in healthy and diseased cats and to determine the occurrence of MDR and MRS spp. as well as possible risk factors associated with colonization in these cats. Five swabs were collected from the anus, skin, ear canal, conjunctival sac, and nares of each cat (209 healthy and 191 diseased) admitted to a veterinary clinic in Eastern Province, Saudi Arabia, between January and December 2018. Prior to sample collection, cat owners completed a questionnaire collecting information on cat demographics, health status, management, and antimicrobial usage. In total, 179 Staphylococcus isolates were recovered from healthy (n = 71) and diseased (n = 108) cats, including 94 (52.5%) coagulase-positive staphylococci (CoPS), and 85 (47.5%) coagulase-negative staphylococci (CoNS). Five Staphylococcus spp. were identified, namely, Staphylococcus aureus, Staphylococcus pseudintermedius, Staphylococcus felis, Staphylococcus capitis, and Staphylococcus saprophyticus. Staphylococcus isolates were most commonly resistant to penicillin (56.4%) and ciprofloxacin (25.7%); however, no isolate was resistant to clindamycin. Thirty (16.8%) Staphylococcus spp. (24 S. aureus and 6 S. pseudintermedius) isolates were MDR, with resistance to up to six different antibiotic classes. Only 17 (9.5%) Staphylococcus spp. (15 methicillin-resistant S. aureus and 2 methicillin-resistant S. pseudintermedius) harbored the mecA gene. Risk factor analysis showed that cats with a history of antibiotic therapy, those raised mainly indoors with a child, and those who visit a veterinary clinic for treatment were at higher risk of MDR and MRS colonization. In conclusion, MDR and MRS were common in healthy and diseased cats in Saudi Arabia. Thus, an effective antimicrobial stewardship program and further studies using a One Health approach are required to investigate the role of cats as vectors for AMR transmission to humans.

Infant Oral Healthcare and Anticipatory Guidance Practices among Dentists in a Pediatric Care Shortage Area.

Objectives To assess dentists' practices and barriers towards infant oral healthcare (IOH) and anticipatory guidance (AG) in eastern Saudi Arabia. Methods A regional, cross-sectional survey was distributed to 340 (323 general dentists (GPs) and 17 pediatric dentists (PDs)) working in a governmental setting in eastern Saudi Arabia. A 23 close-ended, pilot-tested questionnaire was developed. The questionnaire asked about dentists' IOH and AG practices. A five-point Likert scale question assessed barriers interfering with AG practices. Descriptive and multivariate logistic regressions were used. Results Participation rate was 98.5% (335/340). Only 18% of GPs indicated performing IOH exams, while 100% of PDs do. About 90% of GPs would see children on a first visit when they are ≥3 years old, whereas 60% of PDs reported seeing one-year-old children. Older practitioners and those performing AG were more likely to perform IOH (OR = 1.8, CI = 1.06–3.1, and OR = 3.84, CI = 1.93–7.65, resp.). The majority of respondents (94%) felt their training did not prepare them to practice AG. “Parents bringing their children for the first time for emergency or existing conditions” was cited by 99% of respondents as a barrier to performing AG. Conclusion Increasing the awareness of GPs and parents about the importance of IOH and AG is crucial in improving children's oral health. Collaboration with pediatricians for early referral of children is equally important in increasing the awareness on prevention principles.

Liquefaction hazards mapping along Rеd Sеa coast, Jеddah city, Kingdom of Saudi Arabia

This study mainly aims to evaluate as well as to develop maps of the liquefaction potential for Jeddah City in the Kingdom of Saudi Arabia. Data is obtained and used from 214 boreholes associated with standard penetration tests (SPT). Through taking into account comparable seismic hazardous situations to amax = 0.1 g, the liquefaction potential index (LPI) is measured. In order to compile the liquefaction hazard map, LPI values are correlated, showing the quantitative aspects of the liquefiable layers and the area of likelihood of induced disturbance. This data was analyzed within the framework of the Geographical Information Systems (GIS) application. The results show that, with the exception of the sites in the Abhur and Al-Hamra districts where the liquefaction potential is moderate to high, the main part of the city of Jeddah belongs to a very low liquefaction potential area. The developed liquefaction hazard maps will serve as helpful guides for land planning and management in Jeddah city. In addition to that, they serve as an example of the evaluation of the liquefaction hazard that can also be found in other developed cities in the seismic-prone areas of southern and eastern Saudi Arabia.

Migratory Wild Birds as a Potential Disseminator of Antimicrobial-Resistant Bacteria around Al-Asfar Lake, Eastern Saudi Arabia.

Migratory wild birds acquire antimicrobial-resistant (AMR) bacteria from contaminated habitats and then act as reservoirs and potential spreaders of resistant elements through migration. However, the role of migratory wild birds as antimicrobial disseminators in the Arabian Peninsula desert, which represents a transit point for birds migrating all over Asia, Africa, and Europe not yet clear. Therefore, the present study objective was to determine antimicrobial-resistant bacteria in samples collected from migratory wild birds around Al-Asfar Lake, located in Al-Ahsa Oasis, Eastern Saudi Arabia, with a particular focus on Escherichia coli virulence and resistance genes. Cloacal swabs were collected from 210 migratory wild birds represent four species around Al-Asfar. E. coli, Staphylococcus, and Salmonella spp. have been recovered from 90 (42.9%), 37 (17.6%), and 5 (2.4%) birds, respectively. Out of them, 19 (14.4%) were a mixed infection. All samples were subjected to AMR phenotypic characterization, and results revealed (14–41%) and (16–54%) of E. coli and Staphylococcus spp. isolates were resistant to penicillins, sulfonamides, aminoglycoside, and tetracycline antibiotics. Multidrug-resistant (MDR) E. coli and Staphylococcus spp. were identified in 13 (14.4%) and 7 (18.9%) isolates, respectively. However, none of the Salmonella isolates were MDR. Of the 90 E. coli isolates, only 9 (10%) and 5 (5.6%) isolates showed the presence of eaeA and stx2 virulence-associated genes, respectively. However, both eaeA and stx2 genes were identified in four (4.4%) isolates. None of the E. coli isolates carried the hlyA and stx1 virulence-associated genes. The E. coli AMR associated genes blaCTX-M, blaTEM, blaSHV, aac(3)-IV, qnrA, and tet(A) were identified in 7 (7.8%), 5 (5.6%), 1 (1.1%), 8 (8.9%), 4 (4.4%), and 6 (6.7%) isolates, respectively. While the mecA gene was not detected in any of the Staphylococcus spp. isolates. Regarding migratory wild bird species, bacterial recovery, mixed infection, MDR, and AMR index were relatively higher in aquatic-associated species. Overall, the results showed that migratory wild birds around Al-Asfar Lake could act as a reservoir for AMR bacteria enabling them to have a potential role in maintaining, developing, and disseminating AMR bacteria. Furthermore, results highlight the importance of considering migratory wild birds when studying the ecology of AMR.

Regional hydrogeology of the Arab-D aquifer in Central and Eastern Saudi Arabia with highlighting its environmental and energy potential

The Jurassic Arab-D reservoir hosts major oil-producing zone in Eastern Saudi Arabia with production from individual wells exceeding 25000 B/D. From a water supply perspective, Saudi water authorities consider it a minor aquifer with small yields of relatively high salinity; hence, it did not receive much attention in previous studies of aquifers. The present paper describes regional hydrogeological characteristics of the Arab-D in terms of groundwater flow, salinity and temperature, and discusses geological factors controlling these characteristics. Although high groundwater salinity in the Arab-D precludes it from being a potential water supply resource, studying the hydrogeology of Arab-D provides a hydrodynamic tool to oil exploration and development project. It also sets the stage for detailed studies of Arab-D aquifer potential use for industrial effluent disposal and as new geothermal energy source. Most of groundwater flow in Arab-D occurs in the porous and permeable grainy-textured upper zones. The top limit of the groundwater flow domain in Arab-D is the overlying massive Arab-D anhydrite unit. Trends in Arab-D hydraulic head, water salinity and temperature show gravity-driven flow towards the coastal region of Eastern Arabia. Major anomalies in these trends exist near the Ghawar, Summan, Jauf and Yabrin north-south faults. These anomalies are results of fault zone architecture that consists of sealing fault core zones surrounded by conductive fault damage zones. Depth, salinity, and temperature of the Arab-D between north Ghawar and Berri fields show that it has potential uses for industrial effluent disposal and geothermal energy projects.

The Middle East respiratory syndrome coronavirus (MERS-CoV) nucleic acids detected in the saliva and conjunctiva of some naturally infected dromedary camels in Saudi Arabia -2019.

Dromedary camels are playing essential roles in the evolution and transmission of MERS-CoV. MERS-CoV shedding in some dromedary camel secretions, particularly nasal swabs, were studied in more detail. However, the roles of viral shedding in saliva and ocular secretions are still required further detailed studies. We performed a longitudinal study on a farm of dromedary camel herd from 10th March until 7th April, 2019, in eastern Saudi Arabia. This is a closed management herd including a large number of colour-based breed animals and include animals of both sexes. We collected saliva and ocular swabs from 18% of the target animal population. Detection of the MERS-CoV-RNAs in these samples was conducted by the real-time PCR technique. We detected the viral RNAs in the saliva of and conjunctival swabs of some of the tested animals at 33%, 77% and 88% during the three-time points, respectively. Moreover, we also detected the viral RNAs in the conjunctival swabs at 11%, 22% and 33% at similar time intervals. Our results are suggesting the possibility of MERS-CoV shedding in the saliva and the ocular discharges of the infected dromedary camels. This explains, at least in part, the mechanism of transmission of MERS-CoV from animals to humans. More studies are needed for a better understanding of the transmission of MERS-CoV from animals to humans; thus, the risk of virus spread can be mitigated.

Mixed carbonate-siliciclastic sequence development influenced by astronomical forcing on a distal foreland, Miocene Dam Formation, eastern Saudi Arabia

Given increasing importance of tertiary reservoirs in the Middle East, the Miocene (Upper Aquitanian to mid-Langhian) Dam Formation in eastern Saudi Arabia was cored along a 40 km transect, to develop a high-resolution sequence framework, and examine the role played by astronomical forcing, moderate glacio-eustasy (20- to 40-m sea-level changes), and tectonic deformation in this distal foreland setting. The Dam Formation (up to 80 m thick) contains updip siliciclastics which interfinger downdip with marine carbonates. Siliciclastic facies include paleosol-capped, red mudrock (mudflat), green and gray mudrock (near-shore siliciclastic lagoon), rare mud clast–bearing sandstone (fluvial), and massive very fine to medium sandstone (terrestrial sand sheets, rare tidal channels). Carbonate facies include brecciated carbonates (paleosols), lime/clay-clast quartz wackestone (lag gravel), carbonate laminites and microbial heads and mounds (tidal flat and shallow subtidal), argillaceous quartzose marl (near-shore lagoon), peloid mudstone-wackestone (restricted lagoon), variably quartzose skeletal peloid mudstone to packstone (open lagoon), oolitic grainstone (hypersaline beach, tidal channel), mollusk packstone (hypersaline lagoon border), and foram-mollusk packstone-grainstone (seagrass meadows). The Dam Formation contains eight sequences, each of which contains 2 to 4 parasequence sets or individual parasequences. Sequences and individual parasequences are capped by paleosols and/or erosion surfaces, and microbial laminite caps are rare. Bases of parasequences locally are veneered with ravinement gravel lags or siliciclastics beneath shallowing-upward subtidal carbonates, and lack intercalated deeper water facies. Statistical analysis of core-gamma ray (API) vs depth (m) shows that accumulation rates were ~ 3 to 4 cm/kyr, considerably faster than long-term accommodation rates (~ 1.2 cm/kyr), indicating deposition occurred for only 40% of the time, the remainder of the time being non-depositional during many short-term lowered sea levels, evidenced by the coeval oceanic δ18O curve. Spectral analysis of the core-gamma ray logs show astronomical forcing within the eccentricity, obliquity, and precessional bands. Spectral analysis indicates that the eight Dam sequences (average 10 m thick, range < 5 to 18 m) are a mix of long-term obliquity (~ 1.2 Myr) and long-term eccentricity (400 k.y.) cycles. The 3- to 4.4-m sets/parasequences are short term (~ 100 kyr) cycles, and the 0.7- to 1.25-m parasequences are obliquity and precessional cycles. Given the updip position of the study area, repeated glacio-estutatic sea-level changes caused repeated shallow flooding (< 20 m) and emergence, which generated paleosol-bounded or erosionally capped sequences and parasequences. Although the distal foreland was undergoing synsedimentary deformation and slow but locally variable subsidence, which influenced variable thickness of units, astronomically driven glacio-eustasy was the dominant influence on timing of sequence and parasequence development.

Impact of Socio-demographic factors on Quality of life in Medical Students of Eastern Saudi Arabia

Background: Quality of life of medical students may influenced by different socio-demographic components and disrupt their capability to study medicine. An in-depth exploration of these elements may encourage enhancement in learning and retention of medical students.&#x0D; Aim: This study was designed to identify the role of socio-demographic factors in quality of life in undergraduate medical students.&#x0D; Material and Method: A cross-sectional study was conducted among 223 undergraduate medical students (male = 125, females = 98) at King Faisal University, Saudi Arabia between February and September 2019. An Arabic version of WHOQOL-BREF was used to explore the QOL of medical students. Regression analysis were employed to evaluate association between QOL and socio-demographic factors including age, sex, academic year, family type, area of residence, monthly income, parental education and housing status.&#x0D; Result: The results showed a significant relationship between various socio-demographic variables and quality of life. The findings of the study revealed that gender, academic year, marital status, monthly income and family type were found significant predictors of quality of life among medical students.&#x0D; Conclusion: The present study has explored the impact of socio-demographic factors on the quality of life of medical students.&#x0D; Bangladesh Journal of Medical Science Vol.20(2) 2021 p.250-257

Molecular characterization of ticks and tick-borne piroplasms from cattle and camel in Hofuf, eastern Saudi Arabia.

The aims of the present study were to characterize ticks infesting the dromedary camel and cattle in Hofuf, Eastern Saudi Arabia and to determine the piroplasms that they may harbor. DNA was extracted from ticks, collected from camels and cattle, using commercial kits and subjected to polymerase chain reaction using specific primers for the amplification of ticks and piroplasms DNA. The cytochrome oxidase subunit I mitochondrial gene (COI) was used for characterization of ticks whereas partial 18S rRNA gene (18S rRNA) was used for piroplasms characterization. Ticks were genetically identified as Hyalomma dromedarii and Hyalomma anatolicum. Both cattle and camel in Hofuf, were found to be infested with both species. Both ticks identified as H. dromedarii and H. anatolicum from camels and cows showed 100% identity to COI sequences from the same species available in GenBank. Only Theileria annulata DNA was amplified from both H. anatolicum and H. dromedarii infesting cattle. None of the ticks collected from camels revealed DNA of piroplasms. T. annulata DNA was reported for the first time from Hofuf and the role of both H. anatolicum and H. dromedarii as potential vectors for this parasite in cattle in Saudi Arabia has been documented for the first time.

The role of social media in the training and continuing education of healthcare professionals in Eastern Saudi Arabia

Continuing education is essential for healthcare professionals to update their theoretical and practical knowledge about the advances of the medical profession throughout their professional lives. No study has been conducted in Saudi Arabia on this topic. The objective of this study was to assess the role of social media in the training and continuing education of healthcare professionals in Eastern Saudi Arabia. A total of 483 healthcare professionals were randomly selected and administered with a survey through an online application distributed using a link via email, WhatsApp, Facebook, and LinkedIn. The response rate was 72%. 53% of the participants attended training and continuing education programs through social media in Eastern Saudi Arabia. YouTube (23%) was the most used social network for this purpose. The respondents considered that social media platforms were “very helpful” to improve their knowledge (52%), creativity (45%), clinical skills (42%), critical thinking (50%), problem-solving skills (52%), and patient outcomes (44%). The results suggested that it is necessary to develop adequate strategies for healthcare professionals to increase the use of training and continuing education programs through social networks in Saudi Arabia. In general, the participants considered that social networks were beneficial tools to improve their theoretical and practical knowledge and expand their professional training.

Hemodialysis during Lockdown Due to Coronavirus Disease 2019 Pandemic in Eastern Saudi Arabia.

The first case of Coronavirus disease-2019 (COVID-19) in the Kingdom of Saudi Arabia was confirmed in the city of Qatif in March 2020. As a result, Qatif was placed under lockdown for two months in an attempt to prevent the widespread of COVID-19. Doing hemodialysis (HD) during lockdown was a new and challenging experience that we recently have faced. Swift arrangements were made to accommodate patients with end-stage renal disease in need for HD. The challenges to healthcare facilities, healthcare providers, and patients are discussed with the hope that this experience would help mitigate some of the difficulties healthcare providers may face in a similar situation.

A Paucity of Detection of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Nucleic Acids in the Vaginal Secretions and the Urine of Some Naturally Infected Dromedary Camels

The dromedary camels play some critical roles in the evolution and transmission cycle of the MERS-CoV. There are no current available vaccines or antiviral medications to do prevention or to treat humans against the virus, respectively. Few studies reported the course of the MERS-CoV infection in dromedary camels under both the experiment and natural infection approaches. The curve of the viral particle, as well as its nucleic acids in nasal secretions of the dromedary camels, was reported in more details. However, MERS-CoV shedding in some other secretions such as vaginal secretions still understudied. The main goal of the current study was to check the possibility of MERS-CoV shedding of the MERS-CoV through the vaginal mucous membranes of some female camels. To achieve these goals, we conducted molecular surveillance on one dromedary camel herd in eastern Saudi Arabia. We collected the following swabs per each animal (nasal, rectal, and vaginal) as well as urine samples during the period from Jan 2020-April 2020. Testing of MERS-CoV nucleic acid (NA) was conducted in these swabs by the real-time PCR kits. Our results are showing that (47%, 21%, 0%, and 0%) of the (nasal, rectal, and vaginal) swabs, as well as the urine, were positive, respectively. All the tested vaginal swabs were negative. This may suggest the absence of MERS-CoV shedding in the female genital tract and urine samples despite the detection of the viral NA in the nasal swabs. This study is showing for the first time the failure of detection of the MERS-CoV in the vaginal secretions of some naturally infected dromedary camels. This result is enriching our understanding of the MERS-CoV shedding in various body secretions and excretions.

Hearing impairment in military personnel in Eastern Saudi Arabia.

BACKGROUND:Noise exposure is one of the most common occupational hazards worldwide. Studies have shown that the prevalence of hearing loss and tinnitus is higher in military personnel than in other occupations. This study aimed to estimate the prevalence of hearing impairment in military personnel in Eastern Saudi Arabia.MATERIALS AND METHODS:A cross-sectional study was conducted among 409 military personnel. A self-administered questionnaire collected data on level of awareness, hearing impairment, and protection of hearing. Pure-tone audiometry (PTA) was conducted on 141 participants to determine the prevalence and pattern of hearing impairment. Multivariate analysis was used to determine the predictors of hearing loss in military personnel.RESULTS:More than half of the participants (54.3%) were unaware of the consequences of noise exposure and none used proper hearing protection. A small percentage (5.9%) complied with the annual hearing examination, and only 23% had had a previous hearing evaluation. More than half of the participants (58.4%) had one or more abnormal hearing-related symptoms, with tinnitus as the most common symptom (43.8%). PTA showed hearing impairment in 71.6% of the participants. Multivariate analysis showed older age as only significant factor associated with hearing impairment in military personnel.CONCLUSION:Noise-induced hearing loss and tinnitus are common occupational disabilities in military personnel. Hearing conservation programs have to be initiated to ensure the application of hearing protection measures and control the effects of exposure to noise.

Nurses Perception of Advance Nurse Practitioners Roles in Public Hospitals: A Qualitative Study

Aim: To explore the nurses’ perceptions about the advanced nurses’ practitioner’s role in public hospitals in Saudi Arabia. Background: Advanced nurse practitioners are one of the leading professionals who contribute to health promotion, disease prevention, health counseling and patient education. However, misunderstandings of their roles may have an impact on the performances of the function that limits their access and the use of significant resources that can help patients to obtain treatment. Methods: This is a qualitative exploratory study conducted on nurses working in three public hospitals. Semi-structured interviews were conducted among fourteen nurses between October-January 2020. The three public hospitals were based in Eastern (1) and Central (2) Saudi Arabia regions. Data analysis was conducted through thematic analysis utilizing inductive and deductive coding. Results: The study found that the role of Advanced Practice Nurses will enhance, motivate, and sustain the status of nurses in Saudi Arabia. Findings indicated that advanced practitioners’ educational preparedness, organizational, and contextual factors are important factors that need to be addressed to promote their role and to improve nurse’s perception about the APN role in clinical practice. Conclusion: This research provided new evidence to support the need to raise understanding of the role of advanced nurse practitioners among nurse members, advanced nurses and clinical nurse specialists who wish to enhance quality healthcare delivery and job satisfaction in the Kingdom of Saudi Arabia. Recognizing the factors that comprise the function and status of APNs will assist health-care officials to make informed decisions when designing a national policy and a plan for the implementation and the development of the role of APNs.

Drug Resistance-Associated Mutations in ERG11 of Multidrug-Resistant Candida auris in a Tertiary Care Hospital of Eastern Saudi Arabia.

Candida auris is an emerging multi-drug resistant pathogen with high mortality rate; nosocomial infections have been reported worldwide, causing a major challenge for clinicians and microbiological laboratories. The study aims to describe new cases of C. auris and detect drug resistance-associated mutations of C. auris by the sequencing of ERG11 and FKS1 genes. A total of six specimens were collected from blood, urine, ear swab, and groin screening samples. Isolates were incubated for 48 h on Sabouraud Dextrose agar (SDA) at 42 °C, then confirmed by MALDI-TOF MS. Furthermore, antifungal susceptibility testing was performed using the Vitek 2 system to detect Minimum Inhibitory Concentrations (MICs) of six antifungals. Sequences of 18S rRNA gene and ITS regions from isolates and phylogenetic analysis were performed. Gene sequencing was analysed to detect drug resistance-associated mutations by FKS1 and ERG11 genes sequencing. All C. auris isolates were confirmed by MALDI-TOF MS, and evolutionary analyses using sequences of 18S rRNA gene and ITS region. Antifungal susceptibility testing showed that all isolates were resistant to fluconazole. Sequencing of ERG11 and FKS1 genes from the isolates revealed the presence of two (F132Y and K143R) drug resistance-associated mutations in ERG11, however, FKS1 gene was devoid of mutations. The study sheds light on a public health threat of an emerging pathogen, and the hospital implemented strict contact screening and infection control precautions to prevent C. auris infection. Finally, there is a critical need to monitor the antifungal resistance in different geographical areas and implementation of efficient guidelines for treatment.

Effect of harvest season on the nutritional value of bee pollen protein.

Bee pollen is a natural product that has valuable nutritional and medicinal characteristics and has recently garnered increasing attention in the food industry due to its nutritive value. Here, we harvested pollen loads from the Al-Ahsa oasis in eastern Saudi Arabia during spring, summer, autumn, and winter in 2018/2019 to compare the nutritional value of bee pollen protein with the amino acid requirements of honeybees and adult humans. Based on the nutritional value of bee pollen protein, the optimal season for harvesting bee pollen was determined. The composition of the bee pollen showed the highest contents of crude protein, total amino acids, leucine, glutamic acid, valine, isoleucine, threonine, and glycine in samples collected in spring. The highest contents of lysine, phenylalanine, threonine, tryptophan, arginine, tyrosine, and cysteine were observed in samples collected in winter. The highest contents of histidine, methionine, and serine were in samples collected in autumn. Moreover, the highest levels of aspartic acid, proline, and alanine were in samples collected in summer. Leucine, valine, lysine, histidine, threonine, and phenylalanine (except in autumn bee pollen) contents in pollen from all four seasons were above the requirements of honeybees. Leucine, valine, histidine, isoleucine (except in autumn bee pollen), lysine (except in spring and summer bee pollen), and threonine (except in winter and spring bee pollen) in all tested samples were above the requirements of adult humans. In comparison with the minimal amino acid requirements of adult humans and honeybees, the 1st limiting amino acid in bee pollen collected during the different seasons was methionine. Bee pollen collected during spring (March-May) and winter (December-February) can be considered a nutritive food source for adult humans and honeybees.

The Geomechanical and Fault Activation Modeling during CO2 Injection into Deep Minjur Reservoir, Eastern Saudi Arabia

The release of large quantities of CO2 into the atmosphere is one of the major causes of global warming. The most viable method to control the level of CO2 in the atmosphere is to capture and permanently sequestrate the excess amount of CO2 in subsurface geological reservoirs. The injection of CO2 gives rise to pore pressure buildup. It is crucial to monitor the rising pore pressure in order to prevent the potential failure of the reservoir and the subsequent leakage of the stored CO2 into the overburden layers, and then back to the atmosphere. In this paper, the Minjur sandstone reservoir in eastern Saudi Arabia was considered for establishing a coupled geomechanical model and performing the corresponding stability analysis. During the geomechanical modeling process, the fault passing through the Minjur and Marrat layers was also considered. The injection-induced pore-pressure and ground uplift profiles were calculated for the case of absence of a fault across the reservoir, as well as the case with a fault. The stability analysis was performed using the Mohr–Coulomb failure criterion. In the current study, the excessive increase in pore pressure, in the absence of geological faults, moved the reservoir closer to the failure envelope, but in the presence of geological faults, the reservoir reached to the failure envelope and the faults were activated. The developed geomechanical model provided estimates for the safe injection parameters of CO2 based on the magnitudes of the reservoir pore pressure and stresses in the reservoir.