Eastern Nepal Research Articles

Fish Species Composition, Distribution, and Community Structure in the River Kankai, Eastern Nepal

It is essential to track how fishing pressure affects the exploited fish population in the River Kankai to ensure resource development and sustainability. The current state of affairs in the River Kankai, situated in the eastern Nepalese districts of Ilam and Jhapa, continues to raise serious concerns among the general public. The purpose of this study is to determine trends in the fish communities in the River Kankai across time and location and to examine how physicochemical factors affect fish dispersal and abundance at six sites that were chosen based on operability and accessibility. Monthly fish samples were collected between January 2023 and December 2023. We used cast nets in different sizes, monofilament gill nets, fish baits, and local fish traps to collect the fish. Fish abundance and distribution were largely determined by water parameters like turbidity, total hardness, total alkalinity, conductivity, dissolved oxygen, biological oxygen demand, water temperature, and nitrate-N, according to an analysis of variance (ANOVA) on canonical correspondence analysis. Altogether, 94 species in 12 orders, 28 families, and 60 genera were gathered, of which 6 species represented Nearly Threatened (NT) and 3 species were found to be Vulnerable (VU) as per the IUCN red list categories of threatened species. The current study aims to improve the community structure and ichthyofaunal diversity in the River Kankai.

Awareness Regarding Periodontal Health Among Diabetic Patients Visiting A Tertiary Care Centre In Eastern Nepal

Introduction: Diabetes Mellitus and Periodontitis have a bidirectional relationship, each exacerbating the other’s complications. Periodontal disease is considered as the ‘sixth’ complication of diabetes. The adverse relationship between the two diseases is bidirectional. Raising awareness about oral hygiene and treating periodontal issues are essential to mitigate diabetes-related complications and improve overall oral health. Methods: In a descriptive cross-sectional study among a total of 403 diabetic patients visiting out-patient department of Internal Medicine at BP Koirala Institute of Health Sciences, a questionnaire was used to assess awareness of periodontal health among diabetic patients. Results: The results indicated that the general public is less aware of the increased risk of oral diseases in diabetes. Only a minority of the respondents, 127 (31.43%), were aware of the link between poor oral health and the development of disease. 178 (44.05%) believed that diabetic patients are more likely to have swollen/tender gums, and 89 (22.02%) believed that diabetics are more prone to developing fungal infections in their mouth. Furthermore, only 70 (17.32%) of the respondents thought that diabetic patients have gum problems more often if their blood sugar remains high and only 76 (18.81%) believed that gum disease can negatively affect blood sugar control in diabetic patients. Conclusion: These data reveal a lack of knowledge about the oral manifestation of Diabetes Mellitus. Health professionals in dentistry and medicine must collaborate on educational programs to raise awareness about these oral manifestations, aiming to improve oral health and reduce the risk of oral diseases effectively.

Ecosystem services, threats, and management practices of wetlands in Morang District of Nepal

Wetlands are gaining much attention recently as they are threatened by multiple stressors; however, detailed ecological and socio-economic studies on these wetlands are limited. In this study, we present an inventory and assess ecosystem services, and conservation status of the wetlands of Morang district in Eastern Nepal. We used the remote sensing data - Google Earth Imagery and official topographic maps – for preparation of inventory and conducted a rapid field survey using a questionnaire, Key Informant Interviews (KIIs) and Focus Group Discussions (FGDs). Provisional and cultural services were analyzed based on field observations and the questionnaire survey. Twenty-four wetlands of 29.4 ha area, ranging from 0.017 to 7.5 ha were recorded. The Sunbarsi wetland is located in the lowest (87 m) and the Chuli Pokhari in the highest (677 m) elevation. Rajarani wetland of Letang (7.5 ha) is the largest wetland in the district. Ten wetlands have high religious value. Wetlands in Morang district are also productive ecosystems providing different types of ecosystem services. They contribute in local economy providing job and business opportunities to locals. Bhulkey wetland of Pathari-1 provides drinking water to 1,938 families. Further, these wetlands are used for irrigation. The wetlands are also providing services to livestock farming, fishing, boating, commercial activities (hotels and shops), and tourism. Cultural, recreational and ecotourism activities in the wetlands are generating revenue locally.

Assessment of the surface water quality in high-altitude springs in the Sagarmatha (Everest) National Park, Nepal

This study's goal was to examine how the physico-chemical properties of high-altitude springs in eastern Nepal's Sagarmatha National Park and buffer zone changed annually over a period of three years (2008-2010). The majority of the sampling locations were chosen along the treks from Lukla to Everest Base Camp, Gokyo, and Imja valley. Chemical parameters, such as total nitrogen and total phosphorous, were studied in the ecology laboratory of Central Department of Botany, Tribhuvan University, while physical characteristics such as pH, temperature, total dissolved solids (TDS), and conductivity were assessed on the spot by water analyzer kit Although water quality standards still meets the WHO's and Nepal's standards for drinking water, the quality of the spring water is starting to deteriorate. On the main tourist treks, Changes in water quality metrics in water bodies have been observed. Each year, the values of total nitrogen (TN-NO3), total phosphorous (TP-PO4), and pH change, indicating a decline in the quality of the springs water bodies in the Sagarmatha National Park and Buffer Zone (SNPBZ). The values of TN-NO3 and TP-PO4 had significantly increased, indicating that human impact had also increased. Although the amount of total nitrogen in water samples was lower than the WHO limit, there has been an increase in nitrate-nitrogen as compared to past findings. Similar to this, it was discovered that the total phosphorus value increased both annually and in earlier reports. Therefore, sufficient care should be taken to stop future deterioration. The research produced a database for the current state of Nepal's highland springs, which may be used to manage the springs and research how human activity affects water quality.

Assessment of Species Composition, Diversity and Carbon Stock in a Community Managed Forest of Udaypur District of Nepal

Species composition, diversity, and carbon sock of forests are all critical factors that affect the ability of forests to provide various important ecosystem services. However, there is a notable dearth of research regarding these factors in the community forests of Udaypur district. Therefore, this research was undertaken to assess species composition, and biodiversity and quantify the carbon stock potential of the Sringar community forest (CF) of the Udaypur district of Eastern Nepal. A total of 57 circular plots of 500m2 were inventoried using a systematic random sampling method with 0.5 % sampling intensity. In the CF, 17 tree species from 15 genera and 11 families were identified. The dominant tree species, Shorea robusta observed with a maximum importance value index (IVI) (176.15). According to our study, the total biomass and carbon stock in Sringar CF were 276.98 ton ha-1 and 138.18 ton ha-1, respectively. Accurate estimation of soil carbon stocks is crucial for long-term forest management and climate change mitigation, and the integration of advanced monitoring techniques and predictive models to enhance accuracy and account for future climate projections is needed.

Habitat Characteristics of the Endangered Himalayan Red Panda in Panchthar–Ilam–Taplejung Corridor, Eastern Nepal

The Panchthar–Ilam–Taplejung Corridor in Eastern Nepal, managed through community forestry, is a crucial habitat for the Himalayan red panda, an endangered carnivore threatened by forest degradation and illegal trade. We deployed the altitude line intercept and ten-tree plotless methods to evaluate the distribution of Himalayan red pandas and the environmental factors affecting them within four community forests, namely Singhadevi, Chitre-Hile, Chhipchhipe, and Kalikhop-Dadehli, of the corridor. We established a total of 23 transects and 92 plots, identifying 41 plots with evidence of the Himalayan red panda’s presence. The sign occurrence revealed a clumped distribution of the species across all four community forests. The Himalayan red panda signs were observed between 2200 m and 2700 m above sea level (asl) and the majority of them were from habitats with a moderate slope within elevations of 2400 m to 2500 m asl. The primary sites for the defecation were large horizontal tree branches (78.12%), followed by forest ground (15.62%) and rocks (6.25%). The dominant tree species in their habitats included Lithocarpus pachyphylla (Importance value index, IVI = 45.05), Symplocus theifolia (IVI = 37.19), Symplocos pyrifolia (IVI = 20.99), Quercus lamellosa (IVI = 19.25), and Magnolia campbellii (IVI = 17.25). Among the thirteen environmental variables examined, proximity to water, distance to road, bamboo density, and Normalized Difference Vegetation Index were identified as the major factors influencing the Himalayan red panda’s distribution. This research provides crucial insights to develop site-specific habitat management plans for community forestry.

Distribution records of Assamese macaques (Macaca assamensis pelops) from the Bheri River Basin in western Nepal

The Assamese macaque (Macaca assamensis) population in Nepal is one of the least studied mammals. It is legally protected by the National Parks and Wildlife Conservation Act 1973; however, its population status, distribution and conservation threats are ill-documented. The species is thought to have a disjunct distribution having limited information about its occurrence in mid-western and far-western Nepal. This study surveyed the Assamese macaques in the Bheri River Basin of western Nepal and recorded their distribution in the basin for the first time. A total of 117 individuals were observed belonging to six different troops with an average troop size of 19.5 individuals. The elevational range of the distribution ranged between 1132 m and 1840 m asl. The Assamese macaque individuals from the Bheri River Basin are much darker and bear longer relative tail lengths than those of eastern and central Nepal. Further explorations and a detailed assessment of conservation threats in western Nepal are warranted.

Factors Associated with Enrollment of Household in National Health Insurance Program in Eastern Nepal

Background Health insurance is entrusted to make health services more equitable and decrease the financial burden caused by catastrophic out-of-pocket payments. This study aimed to assess the factors affecting the enrollment of households in National Health Insurance Program (NHIP) in eastern Nepal.MethodsCross-sectional data were collected from 189 households in Itahari sub-metropolitan city using a structured questionnaire. Chi-square tests was used to examine the association between NHIP enrollment and selected demographic variables derived from the data.ResultsThe findings showed that 63% of households were enrolled in NHIP and enrollment was associated with education, family type, ethnicity, socio-economic status, prior experience of illness, utilization of health facilities, and knowledge regarding NHIP.Conclusions Variables like education, family type, ethnicity, socio-economic status, prior experience of illness, utilization of health facility, knowledge regarding the annual premium amount, annual benefit ceiling, and renewal time found to be associated with NHIP enrollment.

Bone Marrow Aspiration and Biopsy Findings in Patients with Hematological Disorders in a Tertiary Care Center from Eastern Nepal: An Institutional Experience

Background: Bone marrow study is an important study which is performed when the peripheral blood examination and other laboratory tests and clinical signs and symptoms are suggestive and sometimes inconclusive of both hematological neoplastic and non-neoplastic conditions. The aim of this study was to study about the indications of bone marrow to assess the diagnostic value and comparison of microscopic findings with immunophenotyping and molecular studies. Methods: A total of 102cases were analyzed retrospectively and prospectively from January 2022 to October 2023. All the bone marrow aspiration and trephine biopsy received at the laboratory during this period were included in the study and other cases were analyzed form medical record of the department. Results: Among the 102 cases, mean age of the patient was 48 years and majority were male patients. The age range was between 17-80 years. The male: female ratio was 1.25:1. Weakness was most common presenting complain. Acute leukemia was the most frequent diagnosis (24.5%) followed by myeloproliferative neoplasm (7.8%), primary aplastic anemia (6.9%), myelodysplastic syndrome (5.8%), megaloblastic anemia (4.9%) and plasma cell dyscrasia (3.9%), CLPD (2.9%), ITP (1.9%). Reactive marrow constituted 3.9% and few of the rare diagnosis included hypereosinophilia with PDGFRA mutation, Leishmaniasis etc. Conclusions: The study concludes the bone marrow aspirate and biopsy examination is still the gold standard technique for screening and definite diagnosis of hematological conditions. Immunophenotyping and molecular studies are important for treatment purpose and in case of cytomorphological dilemma.

Prevalence of Malocclusion in Eastern Nepal

Background Malocclusion is the abnormal relationship between upper jaw and lower jaw. It is third most common prevalent oral pathology. Different environment may contribute to malocclusion, so treatment may also vary likewise. Determining the prevalence of malocclusion of different geographic region, ethnic groups and gender helps to identify the distribution of malocclusion, which in turn aids in preventive treatments. This study is an attempt to assess the prevalence of malocclusion among the individuals of eastern Nepal.MethodsData collection was done by direct observation of the patients using probe and mouth mirror. Descriptive cross-sectional study method was used in this research. The malocclusion based on Angle’s classification, overjet, overbite, open bite, crowding, spacing and cross bites were recorded.ResultsA total of 429 patients were included in the study. Among them, 190 (44.3%) were males and 239 (55.7%) were females with male: female ratio 1:1.3. The mean age of patients was found to be 26.92 ±10.61years with the age range of 12- 65 years. Angle’s class I (75.1%) was the most prevalent malocclusion whereas Angle’s class III was observed in 16.6% of total samples followed by Angle’s class II (8.4%). Conclusions The most prevalent malocclusion in our study is Angle’s class I in both male and female patients. Crowding was the most prevalent occlusal traits observed whereas open bite was the least.

First record of Garra kempi Hora, 1921 (Cypriniformes: Cyprinidae) from Lohandra River of Nepal

Nepal holds a high ichthyofaunal diversity, nevertheless, the molecular study of Nepalese fish is still in its early stages. The first record of a cyprinid fish species the Garra kempi Hora, 1921 for Nepal was reported from eastern Nepal’s Lohandra River. Both morphology and molecular data affirmed the existence of G. kempi in Nepal’s aquatic system. This species was previously reported from China (Tibet) and northern India. The maximum likelihood phylogenetic analysis and pairwise genetic distance based on Kimura 2 parameters using cytochrome oxidase subunit 1 (COI) segment sequences (665 bp) also confirmed the identity of the species as G. kempi. The COI sequences of specimens from Nepal formed a monophyletic clade with the sequence of G. kempi from northeastern India and the two contained a pairwise genetic distance of 1.8% only. The new record of G. kempi from Nepal warrants a detailed ichthyofaunal survey for documenting the fish diversity in Nepal.

Large Cardamom Farming in Eastern Nepal: Identifying Strengths, Weaknesses, Opportunities and Threats

Large cardamom, an important cash crop from eastern Nepal with significant potential in international trade, is facing challenges in terms of its production and market price. This study aims to document the perception of local farmers of Ilam district in regard to decreased production of large cardamom. First hand data were obtained through visiting the households of farmers and observing and documenting their daily chores for cardamom farming. Questionnaires were developed and were used in individual interviews (77), focus group discussions (4), and key informant interviews (5). The SWOT analysis revealed that the large cardamom farming possesses significant strengths, including high market price and minimum investment in terms of labor and fertilizer. However, it also faces notable weaknesses, such as vulnerability to diseases and environmental stresses. These strengths and weaknesses present several opportunities, including crop diversification, search for disease-resistant and superior varieties, and the adoption of scientific farming. On the other hand, the major threats encountered were the risk of uncertain return due to market price fluctuations, government oversight, lack of technical and scientific support, and vulnerability due to lack of skilled manpower and resources causing farmers to abandon large cardamom farming.

Flowering Plant Diversity of Differentially Disturbed Region of Terai Sal (Shorea robusta) Forest in Eastern Nepal

Terai Sal (Shorea robusta C.F.Gaertn.) forest, characteristic forest of lowland of Nepal, faces direct and indirect impacts from various anthropogenic activities. The present work aimed to study and document the flowering plant diversity within differentially disturbed sites of Charali Sal forest. Additionally, we accessed the habitat condition, analyzed the invasion status and prioritize the threats faced by the plant communities and recommended ways to mitigate the prevalent threats. We employed purposive sampling technique to sample the forest areas and nested plot design for vegetation sampling. Thirty sample plots, each measuring 10 m × 10 m, were established on two differentially disturbed sites (disturbed and undisturbed) of Charali Sal forest. A total of 111 flowering plant species belonging to 39 families were documented in the sampling sites, with 90 total species (89 identified) in disturbed sites and 86 species (80 identified) in undisturbed sites. Although the disturbed sites contained higher number of flowering plants, the diversity indices (Simpson and Shannon-wiener’s) indicated higher diversity on undisturbed site. Disturbed site exhibited higher human encroachment and higher invasion of alien species compared to undisturbed site. Specific habitat management plans should be devised for the control and eradication of invasive species from the Charali Sal forest. We highly recommend to delimit a buffer zone all around the forest perimeter to regulate local access inside the forest. Collecting and harvesting various forest products should be restricted to the buffer zone, while the inner forest areas should be under careful and strict management. Implementing these strict managemental strategies would be challenging, but it is crucial for preserving the natural integrity of the forest.

Relationship Between Fine Root Biomass and Soil Physico-chemical Properties of Grassland Ecosystem in Bhadrapur Municipality of Jhapa district, Eastern Nepal

This study investigated the distribution of fine root biomass and various soil properties in a grassland ecosystem within Bhadrapur Municipality, Nepal. Soil samples were systematically collected from randomly selected blocks in undisturbed, disturbed, and roadside areas during the late rainy season. Analyses included soil texture, moisture content, temperature, pH, and quantification of fine root biomass (FRB; <5 mm diameter) within the top 15 cm soil depth. Results showed the mean FRB was significantly higher in undisturbed areas (7.77 t/ha) compared to disturbed (7.053 t/ha) and roadside (1.76 t/ha) areas. Across all sites, FRB exhibited a positive correlation with soil moisture content. Soil pH was slightly more acidic in undisturbed versus disturbed and roadside areas. Soil temperature demonstrated a positive correlation with FRB in undisturbed and roadside areas but a negative correlation in disturbed areas. Additionally, FRB negatively correlated with sand content in disturbed and roadside areas. The findings highlight how soil moisture is a key driver of fine root proliferation in this grassland ecosystem. Variations in soil texture, pH, and temperature between sites reveal their influence on root growth dynamics. Understanding these root-soil interactions has implications for sustainable grassland management, biodiversity conservation, and ecosystem resilience under changing environmental conditions.

Lindernia rotundifolia (Linderniaceae), Picria fel-terrae (Linderniaceae), and Limnophila aromatica (Plantaginaceae): three new records for the flora of Nepal

Lindernia rotundifolia (L.) Alston (Linderniaceae), Picria fel-terrae Lour. (Linderniaceae), and Limnophila aromatica (Lam.) Merr. (Plantaginaceae) are newly reported from Jalthal forest, eastern Nepal. Picria Lour. is a new generic record for Nepal. Descriptions of all the species based on Nepalese specimens are provided, along with notes on diagnostic features, color photographs of the species, distribution maps, and notes on habitats.

Role of Prophylactics Antibiotics in Clean Head and Neck Surgery in a Tertiary Care Center of Nepal: A Prospective Comparative Study.

Surgical site infections are well recognized complications of any surgical procedures. In head and neck surgeries, prophylactic antibiotics are commonly used to prevent Surgical site infections, in contaminated and clean contaminated procedures. Guidelines advised against routine antibiotics use in clean surgeries, but in our setting, non-compliance is frequent, resulting excessive antibiotics use. Objective of the study was to compare the Surgical site infections rate between prophylactics antibiotics group and no antibiotics group in clean head and neck surgery. It was a prospective comparative study conducted at a tertiary care centre of eastern Nepal. Patients were assigned to two groups, one receiving prophylactic antibiotics and another receiving no antibiotics. Out of 131 patients, 66 received prophylactic antibiotics and 65 did not. Thyroidectomy was the most common surgery. The surgical site infection rate was 6.06% in antibiotic group and 7.69% in no antibiotic group, the difference was not significant (p=0.744). The risk reduction of surgical site infection with use of antibiotics was 0.0163 and number need to treat was calculated to be 61.35 About 3% of the patients who were administered antibiotics experienced adverse drug reactions. Factors, such as gender, amount of blood loss, smoking, placement of drains had no significant impact on Surgical site infections rate. Prophylactics antibiotics does not offer substantial advantages in preventing surgical site infections and are not advised for such procedures. Moreover, their use increases the financial burden and risk of adverse drug reactions to the patients.

Assessment of canthal index among the Nepalese and Indian MBBS students of a Medical College in Eastern Nepal

Assessment of canthal index among the Nepalese and Indian MBBS students of a Medical College in Eastern Nepal

Microbial biomass carbon in grassland soil aggregates of Biratnagar, eastern Nepal

Microbial biomass carbon in grassland soil aggregates of Biratnagar, eastern Nepal

Sustainable Methods for Growing Turmeric: Evaluating the Effects of Synthetic and Organic Fertilizers on vegetative and reproductive attributes

This study aimed to assess the influence of various bio-fertilizer sources on turmeric (Curcuma longa L.) cultivation in Eastern Nepal. The research was conducted at G.P Koirala College of Agriculture and Research Centre, Sundarharaicha, Morang, Nepal, from April 2023 to January 2024. The experiment utilized a randomized complete block design (RCBD) with seven treatments including T1: Recommended dose (RD) of NPK, T2: Goat manure, T3: Organic manure, T4: Poultry manure, T5: Vermicompost, T6: Farmyard manure, T7: Control, replicated three times. Turmeric cultivation practices were implemented following standard agronomic procedures. The recommended dose of synthetic fertilizer, NPK, exhibited the highest enhancement across multiple vegetative and reproductive growth parameters of turmeric, with notable increases in plant height, leaf number, tillers per plant, primary and secondary fingers per clump, fresh rhizome yield, dry yield, and dry recovery percentage. Among the organic sources, goat manure and poultry manure also showed promising results in enhancing turmeric yield and quality. Specifically, NPK recorded the highest fresh rhizome yield at 21.30 tons/ha, while goat manure and poultry manure yielded 20.35 tons/ha and 18.69 tons/ha, respectively. In contrast, the lowest fresh rhizome yield was observed in the control group, indicating minimal enhancement in yield without fertilizer supplementation. The results highlight how organic farming methods may be a good substitute for traditional chemical fertilizers in the context of sustainable turmeric production.

Breeding ecology of the Asian openbill in eastern Nepal: Larger trees support higher fledgling success.

Colonial nesting waterbirds in agricultural landscapes have historically received limited research attention, especially in South Asia. For example, the Asian openbill (Anastomus oscitans) is a colonial species that extensively utilizes agricultural landscapes, yet there is a notable lack of substantial studies despite increasing urbanization across these landscapes. We investigated the factors affecting the breeding ecology of Asian openbills in eastern Nepal. We used a grid-based approach to locate stork colonies and monitored them throughout the breeding season from May to November for three consecutive years (2020-2022). Altogether, we observed a total of 67 active colonies, comprising 4020 active nests, which successfully fledged a total of 7566 chicks. Additionally, most of the colonies were located within areas of human settlements (40%), followed by community forests (33%) and agricultural land (27%). The Asian openbills primarily preferred large trees, such as Bombax ceiba (72%), for nesting. The mean height of nesting trees was approximately 4 m taller, the diameter at breast height was twice as large, and the canopy cover area was three times greater than that of non-nesting trees. The canopy cover area of trees positively influenced the colony size, while colony size positively influenced the fledgling's success. Our study underscores the significance of large trees in providing sufficient space for accommodating a substantial number of openbill nests and fledglings. These findings have significant implications for conservation efforts to protect large trees along with wetlands and agricultural lands, as crucial measures to ensure the sustainable breeding of this nationally vulnerable species.