Objectives. This research aimed to study the prevalence and perceived discomfort of musculoskeletal (MSK) symptoms among short-distance and long-distance deep-seaport truck drivers. Methods. Cross-sectional analysis using a standardized modified version of the Nordic musculoskeletal questionnaire (NMQ) was carried out using direct interviews with 25 male participants: 15 short-distance and 10 long-distance truck drivers. Results. As much as 88% was reported for the existence of MSK symptoms in the past 12 months. Considering all truck groups, regardless of short or long distance, the lower back was found with the highest prevalence (72%) followed by the neck (32%). The χ2 test showed long-distance truck drivers had statistically significantly more prevalence in the neck (p = 0.028) than short-distance drivers. Perceived discomfort by the Borg CR10 scale confirmed the lower back had the highest score (2.4) followed by the neck (1.44). The Kruskal–Wallis test revealed that long-distance truck drivers had significantly higher scores on the lower back and neck (p = 0.039 and p = 0.009, respectively). Conclusion. Longer exposure to prolonged non-natural working postures, vibration, traffic conditions and working stress could be the judicial causes. To minimize this problem, integrated interventions need to be implemented with particular measures among short-distance and long-distance truck drivers.