Here we presented the new data and new records of Lepidoptera (Crambidae, Cossidae, Lasiocampidae, Drepanidae, Geometridae, Notodontidae, Noctuidae s.l., Arctiidae) from the Khakassia Republic and the South of Krasnoyarsk region. A total of 147 species are reported for the fauna of both regions for the first time, based on the literature records and personal data collected in 1999−2019. We collected the moths in Khakassia and South of Krasnoyarsk region in more than 40 localities, from the steppe zone to the alpine area of the West Sayan, the Kuznetsky Alatau, and foothills of East Sayan Mountains. Specimens were collected in the daylight by standard methods using butterfly net and in the night, using mercury lamp Sylvania (250W) and inverter generator (1000W). All collected materials preserved in private collections of R.E. Maksimov in Abakan (RMA), M.A. Ivanov in Krasnoyarsk (MIK), and in private collection of S.A. Knyazev in Omsk (SKO). The presence of several species have been considered doubtful before our study. We suggested that the regional list of Heterocera of Khakassia and South of the Krasnoyarsk region should be considerably revised. The great share of the specimens mentioned in this article is presented on the websiite “The Nature of South Siberia” (www.ermak24.com/animalia-insecta.html/).