East Asian Samples Research Articles

Systematic review and meta-analysis of serotonin transporter genotype and discontinuation from antidepressant treatment

There is evidence that 5-HTTLPR is associated with response following treatment from selective serotonin reuptake inhibitors (SSRIs). The short (S) allele has reduced serotonin transporter expression, compared to the long (L) allele, and has been reported to be associated with poorer response in Europeans, with the effect in other populations unclear. However the published literature is inconsistent. A systematic review and meta-analysis was performed to investigate the effect of 5-HTTLPR on discontinuation from antidepressant treatment. Data were obtained from 17 studies including 4309 participants. The principal outcome measure was the allelic odds ratio (OR) for the 5-HTTLPR S allele and discontinuation status. A random effects meta-analysis provided no evidence that the S allele was associated with increased odds of discontinuation from SSRIs in Europeans (OR 1.09, 95% CI 0.83–1.42, p=0.53; 10 studies, n=2504) but in East Asians there was evidence of a reduced odds of discontinuation (OR 0.28, 95% CI 0.12–0.64, p=0.002; 2 studies, n=136). There was a suggestion of small study bias (p=0.05). This meta-analysis provides no evidence of an association between 5-HTTLPR and discontinuation from antidepressant treatment in Europeans. The low number of studies in East Asian samples using SSRIs reduces confidence in our evidence that the S allele decreases the odds of discontinuation in this population. At present, there is no evidence of an association between 5-HTTLPR and discontinuation from SSRI treatment in a European population with further studies required to investigate its effects in different populations.

Insights into Korean Red Fox (Vulpes vulpes) Based on Mitochondrial CytochromebSequence Variation in East Asia

The red fox (Vulpes vulpes) is the most widely distributed terrestrial carnivore in the world, occurring throughout most of North America, Europe, Asia, and North Africa. In South Korea, however, this species has been drastically reduced due to habitat loss and poaching. Consequently, it is classified as an endangered species in Korea. As a first step of a planned red fox restoration project, preserved red fox museum specimens were used to determine the genetic status of red foxes that had previously inhabited South Korea against red foxes from neighboring countries. Total eighty three mtDNA cytochrome b sequences, including 22 newly obtained East Asian red fox sequences and worldwide red fox sequences from NCBI, were clustered into three clades (i.e., I, II, and III) based on haplotype network and neighbor-joining trees. The mean genetic distance between clades was 2.0%. Clade III contained South Korean and other East Asian samples in addition to Eurasian and North Pacific individuals. In clade III, South Korean individuals were separated into two lineages of Eurasian and North Pacific groups, showing unclear phylogeographic structuring and admixture. This suggests that South Korean red fox populations may have been composed of individuals from these two different genetic lineages.

Betel-quid dependence and oral potentially malignant disorders in six Asian countries

Despite gradual understanding of the multidimensional health consequences of betel-quid chewing, information on the effects of dependent use is scant. To investigate the 12-month prevalence patterns of betel-quid dependence in six Asian populations and the impact of this dependence on oral potentially malignant disorders (OPMD). A multistage random sample of 8922 participants was recruited from Taiwan, mainland China, Indonesia, Malaysia, Sri Lanka and Nepal. Participants were evaluated for betel-quid dependency using DSM-IV and ICD-10 criteria and assessed clinically for oral mucosal lesions. The 12-month prevalence of dependence was 2.8-39.2% across the six Asian samples, and 20.9-99.6% of those who chewed betel-quid were betel-quid dependent. Men dominated the prevalence among the east Asian samples and women dominated the prevalence in south-east Asian samples. 'Time spent chewing' and 'craving' were the central dependence domains endorsed by the Chinese and southern/south-east Asian samples respectively, whereas the Nepalese samples endorsed 'tolerance' and 'withdrawal'. Dependency was linked to age, gender, schooling years, drinking, smoking, tobacco-added betel-quid use and environmental accessibility of betel-quid. Compared with non-users, those with betel-quid dependency had higher pre-neoplastic risks (adjusted odds ratios 8.0-51.3) than people with non-dependent betel-quid use (adjusted odds ratio 4.5-5.9) in the six Asian populations. By elucidating differences in domain-level symptoms of betel-quid dependency and individual and environmental factors, this study draws attention to the population-level psychiatric problems of betel-quid chewing that undermine health consequences for OPMD in six Asian communities.

The validity of a three-factor model in PPI-R and social dominance orientation in Japanese sample

The Psychopathic Personality Inventory-Revised (PPI-R) is one of the widely used instruments for assessing psychopathy in subclinical samples. While a three-factorial model of PPI-R has been tested in US, no studies have examined the structure and correlates of the PPI-R scale in an East Asian sample. This study examined the validity of the PPI-R in a Japanese subclinical sample, and investigated correlations between subscales of psychopathy measured by PPI-R and social dominance orientation (SDO). One hundred and sixty undergraduate students in Japan participated in a survey. Two main findings revealed that (1) the factorial structure of the PPI-R in Japan was different from that found in previous studies in the United States, and (2) the first factor, including Machiavellianism, and the second factor, including Coldheartedness, were linked with SDO. The role of intergroup aggression in psychopathy is also discussed.

Forensic performance of two insertion–deletion marker assays

Improving the amplification and analysis of highly degraded DNA extracts has been a longstanding area of research in forensic genetics. One of the most promising recent developments in analysis of degraded DNA is the availability of short, biallelic insertion-deletion length polymorphisms (InDels) in highly multiplexed assays. InDels share many of the favourable characteristics of single-nucleotide polymorphisms (SNPs) that make them ideal markers for analysis of degraded DNA, including: analysis in short amplicon size ranges, high multiplexing capability and low mutation rates. In addition, as length-based polymorphisms, InDels can be analysed with the same simple dye-labelled PCR primer methods as standard forensic short tandem repeats. Separation and detection of fluorescently dye-labelled PCR products by capillary electrophoresis eliminate the multiple step protocols required by SNP typing with single-base extension assays and provide a closer relationship between the input DNA and the profile peak height ratios. Therefore InDel genotyping represents an effective new approach for human identification that adds informative new loci to the existing battery of forensic markers. To assess the utility of InDels for forensic analysis, we characterised population variation with two InDel identification assays: the 30-plex Qiagen DIPplex panel and a 38-plex panel developed by Pereira et al. in 2009. Allele frequencies were generated for the 68 markers in US African American, Caucasian, East Asian and Hispanic samples. We made a thorough assessment of the individual and combined performance of the InDel sets, as well as characterising profile artifacts and other issues related to the routine use of these newly developed forensic assays based on artificially degraded DNA and mixed source samples.

Tarsal and tarsometatarsal coalitions from Mound C (Ocmulgee Macon Plateau site, Georgia): Implications for understanding the patterns, origins, and antiquity of pedal coalitions in Native American populations

Tarsal and tarsometatarsal coalitions are malsegmentation errors that result in incomplete division between two or more normally separate bones of the foot. Coalitions may be osseous, characterized by bony union, or non-osseous, in which the affected elements are united by fibrous tissue, cartilage, or some combination of both. Evidence indicates that non-osseous coalitions are frequently overlooked or misinterpreted in skeletal samples. The purpose of this study is to (1) report two non-osseous coalition cases (naviculocuneiform I, CF3-MT3) from the Ocmulgee Mound Site in Georgia, and (2) examine the occurrence of coalitions throughout the foot in Native American samples relative to other major populations. Evidence suggests that Native Americans exhibit a pattern of coalitions in the foot that differs from that recently documented for European and African samples. Native Americans display a relatively high rate of midfoot and forefoot coalitions, and known cases are both geographically and temporally diverse. This distribution, along with evidence of similar patterns in East Asian samples, suggests that the pattern of coalition seen in Native Americans has origins in Asiatic parent populations during the late Pleistocene. Individuals migrating to the New World with proximal midfoot coalitions are likely to have endured biomechanical stress during prolonged physical activity and walking, as frequently seen in modern clinical cases.

Rushton’s contributions to the study of mental ability

This essay describes Rushton’s contribution to examining the nexus of intelligence, race, and genetics, specifically what I termed “Spearman’s hypothesis”. It states that Black–White differences are “most marked in just those [tests] which are known to be saturated with g”. I (Jensen) had confirmed this hypothesis using large data sets in the 1970s and 1980s and also found that Black–White differences were most marked on the more heritable rather than the more cultural subtests. Rushton confirmed and extended these findings in many highly innovative ways and demonstrated Spearman’s hypothesis applied among samples of Gypsy Roma in Serbia, and East Asian, European, South Asian, Colored and Black samples in South Africa. He has not only documented group differences in brain size, intelligence, life span, family structure, infant mortality, developmental precocity, personality, and temperament, and rates of two egg twinning, and crime among East Asians, Europeans, and Africans, but also provided a life history theory that explains them.

Warriors and Peacekeepers: Testing a Biosocial Implicit Leadership Hypothesis of Intergroup Relations Using Masculine and Feminine Faces

This paper examines the impact of facial cues on leadership emergence. Using evolutionary social psychology, we expand upon implicit and contingent theories of leadership and propose that different types of intergroup relations elicit different implicit cognitive leadership prototypes. It is argued that a biologically based hormonal connection between behavior and corresponding facial characteristics interacts with evolutionarily consistent social dynamics to influence leadership emergence. We predict that masculine-looking leaders are selected during intergroup conflict (war) and feminine-looking leaders during intergroup cooperation (peace). Across two experiments we show that a general categorization of leader versus nonleader is an initial implicit requirement for emergence, and at a context-specific level facial cues of masculinity and femininity contingently affect war versus peace leadership emergence in the predicted direction. In addition, we replicate our findings in Experiment 1 across culture using Western and East Asian samples. In Experiment 2, we also show that masculine-feminine facial cues are better predictors of leadership than male-female cues. Collectively, our results indicate a multi-level classification of context-specific leadership based on visual cues imbedded in the human face and challenge traditional distinctions of male and female leadership.

Technical note: Quantitative measures of iris color using high resolution photographs

Our understanding of the genetic architecture of iris color is still limited. This is partly related to difficulties associated with obtaining quantitative measurements of eye color. Here we introduce a new automated method for measuring iris color using high resolution photographs. This method extracts color measurements in the CIE 1976 L*a*b* (CIELAB) color space from a 256 by 256 pixel square sampled from the 9:00 meridian of the iris. Color is defined across three dimensions: L* (the lightness coordinate), a* (the red-green coordinate), and b* (the blue-yellow coordinate). We applied this method to a sample of individuals of diverse ancestry (East Asian, European and South Asian) that was genotyped for the HERC2 rs12913832 polymorphism, which is strongly associated with blue eye color. We identified substantial variation in the CIELAB color space, not only in the European sample, but also in the East Asian and South Asian samples. As expected, rs12913832 was significantly associated with quantitative iris color measurements in subjects of European ancestry. However, this SNP was also strongly associated with iris color in the South Asian sample, although there were no participants with blue irides in this sample. The usefulness of this method is not restricted only to the study of iris pigmentation. High-resolution pictures of the iris will also make it possible to study the genetic variation involved in iris textural patterns, which show substantial heritability in human populations.

Polybrominated Diphenyl Ethers vs Alternate Brominated Flame Retardants and Dechloranes from East Asia to the Arctic

Marine boundary layer air and seawater samples taken during a polar expedition cruise from East China Sea to the Arctic were analyzed in order to compare the occurrence, distribution, and fate of the banned polybrominated diphenyl ethers (PBDEs) with their brominated alternatives as well as the chlorinated Dechloranes. The sum of PBDEs (∑(10)PBDEs) in the atmosphere ranged from 0.07 to 8.1 pg m(-3) with BDE-209 being the dominating congener and from not detected (n.d.) to 0.6 pg L(-1) in seawater. Alternate brominated flame retardants (BFRs), especially hexabromobenzene (HBB), (2,3-dibromopropyl-2,4,6-tribromophenyl ether (DPTE), pentabromotoluene (PBT), 2-ethylhexyl 2,3,4,5-tetrabromobenzoate (EHTBB), bis-(2-ethylhexyl)-tetrabromophthalate (TBPH), were detected in higher concentrations than PBDEs, even in the high Arctic (0.6 to 15.4 pg m(-3) for sum of alternate BFRs), indicating the change of PBDEs toward alternate BFRs in the environmental predominance. In addition, Dechlorane Plus (DP) as well as Dechlorane 602, 603, and 604 were detected both in the atmosphere and in seawater. The highest concentrations as well as the highest compound variability were observed in East Asian samples suggesting the Asian continent as source of these compounds in the marine environment. The air-seawater exchange indicates strong deposition, especially of alternate BFRs, as well as dry particle-bound deposition of BDE-209 into the ocean.

Temperature-dependent recruitment delay of the Japanese glass eel Anguilla japonica in East Asia

Japanese eels spawn mainly during June–August. The larvae (leptocephali) then drift for 3–5 months before metamorphosing into glass eels. The recruitment season generally starts in southern East Asia in November and in northern areas in April the following year, a lag of ~5 months. However, analysis of otolith daily growth rings revealed only a 1–2-month difference in the mean leptocephalus stage between southern and northern East Asian samples. Experiments and field observation indicate that glass eels may starve, lose body weight, and remain in early pigmentation stage for a few months in cold waters. The time lag in recruitment can be accounted for by a longer leptocephalus stage combined with a low temperature-driven delay to upstream migration in winter. The leptocephalus duration and oceanic currents determine the dispersal locations up to the glass eel phase, while temperatures determine the timing of upstream migration time at each location.

Association of vitamin D binding protein (VDBP) polymorphisms and serum 25(OH)D concentrations in a sample of young Canadian adults of different ancestry

Variants of the vitamin D binding protein ( VDBP) gene appear to be associated with levels of the main circulating vitamin D metabolite, 25-hydroxyvitamin D [(25(OH)D]. We examined the associations between the common variants of the VDBP ( GC) gene and concentrations of 25(OH)D in a sample of young Canadian adults of East Asian, European and South Asian ancestry, taking into account the effect of vitamin D intake, skin pigmentation, sex, BMI, sun exposure and season. Three hundred and fifty-one (351) healthy young adults were genotyped for two non-synonymous single nucleotide polymorphisms (SNPs), T436K (rs4588) and D432E (rs7041), using a method that ascertains the GC diplotypes of each individual. After controlling for relevant predictor variables in multiple regression models, the number of GC-2 (436 K) alleles was found to be associated with lower 25(OH)D concentrations in the East Asian sample at fall and winter visits. The number of GC-2 alleles also showed a significant negative association with fall 25(OH)D concentration in the European sample. No associations were noted between the number of GC-2 alleles and 25(OH)D in the South Asian sample at either season. Vitamin D intake was also significantly predictive of serum 25(OHD) concentrations, and similarly to what was observed for the GC polymorphisms, the relative strength of the association was influenced by ancestry and season.

Meta-analysis of genome-wide association studies identifies common variants associated with blood pressure variation in east Asians

We conducted a meta-analysis of genome-wide association studies of systolic (SBP) and diastolic (DBP) blood pressure in 19,608 subjects of east Asian ancestry from the AGEN-BP consortium followed up with de novo genotyping (n = 10,518) and further replication (n = 20,247) in east Asian samples. We identified genome-wide significant (P < 5 × 10(-8)) associations with SBP or DBP, which included variants at four new loci (ST7L-CAPZA1, FIGN-GRB14, ENPEP and NPR3) and a newly discovered variant near TBX3. Among the five newly discovered variants, we obtained significant replication in the independent samples for all of these loci except NPR3. We also confirmed seven loci previously identified in populations of European descent. Moreover, at 12q24.13 near ALDH2, we observed strong association signals (P = 7.9 × 10(-31) and P = 1.3 × 10(-35) for SBP and DBP, respectively) with ethnic specificity. These findings provide new insights into blood pressure regulation and potential targets for intervention.

Dietary Sources of Sodium in China, Japan, the United Kingdom, and the United States, Women and Men Aged 40 to 59 Years: The INTERMAP Study

Public health campaigns in several countries encourage population-wide reduced sodium (salt) intake, but excessive intake remains a major problem. Excessive sodium intake is independently related to adverse blood pressure and is a key factor in the epidemic of prehypertension/hypertension. Identification of food sources of sodium in modern diets is critical to effective reduction of sodium intake worldwide. We used data from the INTERMAP Study to define major food sources of sodium in diverse East Asian and Western population samples. INTERMAP is an international, cross-sectional, epidemiologic study of 4, 680 individuals ages 40 to 59 years from Japan (four samples), People's Republic of China (three rural samples), the United Kingdom (two samples), and the United States (eight samples); four in-depth, multipass 24-hour dietary recalls/person were used to identify foods accounting for most dietary sodium intake. In the People's Republic of China sample, most (76%) dietary sodium was from salt added in home cooking, about 50% less in southern than northern samples. In Japan, most (63%) dietary sodium came from soy sauce (20%), commercially processed fish/seafood (15%), salted soups (15%), and preserved vegetables (13%). Processed foods, including breads/cereals/grains, contributed heavily to sodium intake in the United Kingdom (95%) and the United States (for methodological reasons, underestimated at 71%). To prevent and control prehypertension/hypertension and improve health, efforts to remove excess sodium from diets in rural China should focus on reducing salt in home cooking. To avoid excess sodium intake in Japan, the United Kingdom, and the United States, salt must be reduced in commercially processed foods.

Paleoparasitological report on Ascaris aDNA from an ancient East Asian sample

In this study, Ascaris DNA was extracted and sequenced from a medieval archaeological sample in Korea. While Ascaris eggs were confirmed to be of human origin by archaeological evidence, it was not possible to pinpoint the exact species due to close genetic relationships among them. Despite this shortcoming, this is the first Ascaris ancient DNA (aDNA) report from a medieval Asian country and thus will expand the scope of Ascaris aDNA research.

Female-to-Male Breeding Ratio in Modern Humans—an Analysis Based on Historical Recombinations

Was the past genetic contribution of women and men to the current human population equal? Was polygyny (excess of breeding women) present among hominid lineages? We addressed these questions by measuring the ratio of population recombination rates between the X chromosome and the autosomes, rho(X)/rho(A). The X chromosome recombines only in female meiosis, whereas autosomes undergo crossovers in both sexes; thus, rho(X)/rho(A) reflects the female-to-male breeding ratio, beta. We estimated beta from rho(X)/rho(A) inferred from genomic diversity data and calibrated with recombination rates derived from pedigree data. For the HapMap populations, we obtained beta of 1.4 in the Yoruba from West Africa, 1.3 in Europeans, and 1.1 in East Asian samples. These values are consistent with a high prevalence of monogamy and limited polygyny in human populations. More mutations occur during male meiosis as compared to female meiosis at the rate ratio referred to as alpha. We show that at alpha not equal 1, the divergence rates and genetic diversities of the X chromosome relative to the autosomes are complex functions of both alpha and beta, making their independent estimation difficult. Because our estimator of beta does not require any knowledge of the mutation rates, our approach should allow us to dissociate the effects of alpha and beta on the genetic diversity and divergence rate ratios of the sex chromosomes to the autosomes.

Asian Risk Seeking and Overconfidence1

Weber &amp; Hsee (1998) proposed the “cushion effect” as an explanation of the higher risk taking observed in some East Asian samples, relative to Western samples. Yates, Lee, &amp; Shinotsuka (1992) proposed that the overconfidence observed in most Asian countries, relative to Western countries, reflects differences in the number of arguments typically recruited in those countries. This study examined the 2 seemingly separated domains of research: risk taking and overconfidence. It compared individual and collective (both family and group) decisions among Chinese in Singapore. This permitted tests of both the cushion and the argument recruitment hypotheses. The overall results obtained no support for the cushion effect and partial support for the argument recruitment hypotheses.

Detecting Ancient Admixture and Estimating Demographic Parameters in Multiple Human Populations

We analyze patterns of genetic variation in extant human polymorphism data from the National Institute of Environmental Health Sciences single nucleotide polymorphism project to estimate human demographic parameters. We update our previous work by considering a larger data set (more genes and more populations) and by explicitly estimating the amount of putative admixture between modern humans and archaic human groups (e.g., Neandertals, Homo erectus, and Homo floresiensis). We find evidence for this ancient admixture in European, East Asian, and West African samples, suggesting that admixture between diverged hominin groups may be a general feature of recent human evolution.

Biological affinities of Okhotsk-culture people with East Siberians and Arctic people based on dental characteristics

This paper examines the population affinity of the Okhotsk-culture people who settled along the northeastern coastal areas of the islands of Hokkaido and Sakhalin during the 5th and 11th centuries, on the bases of dental metric and nonmetric features. Based on neighbor-joining analyses of both metric and nonmetric data, the Okhostk people, eastern Siberians, and subarctic American natives were linked more closely to each other than to other East Asian and Pacific samples. In particular, the nonmetric data analysis demonstrated the close linkage between the Okhotsk people and tribes in the Amur River Basin, suggesting that the Okhotsk people were descended from eastern Siberians who might share common ancestry with the present-day Amur people and subarctic Americans. Beside the above demonstration of Okhotsk affinities among the populations settling along the Circum Sea of the Okhotsk rim, as far as the metric dental traits are concerned, close resemblances were found between the Okhotsk, Neolithic Jomon, and Hokkaido Ainu. However, with regard to their overall dental characteristics, it is not clear whether such odontmetric similarities indicate the evidence of substantial genetic interaction between the Okhotsk and Jomon–Ainu lineages, because their nonmetric traits data indicate a quite distant relationship from each other.

On Pancultural Self-Enhancement

Taiwanese participants made better-than-average judgments on collectivistic and individualistic traits, evaluated the personal importance of those traits, and completed measures of psychological adjustment (depression, perceived stress, subjective well-being, and satisfaction with life). Replicating findings from other East Asian samples, participants self-enhanced (i.e., regarded the self as superior to peers) more on collectivistic than individualistic attributes and assigned higher personal importance to the former than the latter. Moreover, better adjusted participants manifested a stronger tendency to self-enhance on personally important attributes. These data are consistent with the view that self-enhancement is a universal human motive that is expressed tactically and at odds with the assertion that self-enhancement is a uniquely Western phenomenon.