East Asian American Research Articles

Cigarette smoking behaviors and the importance of ethnicity and genetic ancestry

Cigarette smoking contributes to numerous diseases and is one of the leading causes of death in the United States. Smoking behaviors vary widely across race/ethnicity, but it is not clear why. Here, we examine the contribution of genetic ancestry to variation in two smoking-related traits in 43,485 individuals from four race/ethnicity groups (non-Hispanic white, Hispanic/Latino, East Asian, and African American) from a single U.S. healthcare plan. Smoking prevalence was the lowest among East Asians (22.7%) and the highest among non-Hispanic whites (38.5%). We observed significant associations between genetic ancestry and smoking-related traits. Within East Asians, we observed higher smoking prevalence with greater European (versus Asian) ancestry (P = 9.95 × 10−12). Within Hispanic/Latinos, higher cigarettes per day (CPD) was associated with greater European ancestry (P = 3.34 × 10−25). Within non-Hispanic whites, the lowest number of CPD was observed for individuals of southeastern European ancestry (P = 9.06 × 10−5). These associations remained after considering known smoking-associated loci, education, socioeconomic factors, and marital status. Our findings support the role of genetic ancestry and socioeconomic factors in cigarette smoking behaviors in non-Hispanic whites, Hispanic/Latinos, and East Asians.

Восточно-азиатская – восточно-североамериканская дизъюнкция в видовом комплексе Onoclea sensibilis: викариантность или перипатрия?

Disjunction between East Asian and eastern North American plants has been recognized since the time of Darwin. Although there is considerable evidence for congruent vicariance associated with late Neogene/Quaternary cooling among angiosperms, similar studies among specific fern families (e.g., Osmundaceae Martinov) have appeared incongruous with this pattern. Pteridologists continue to debate whether long-distance dispersal of wind-blown spores could have produced intercontinental disjunctions among ferns. To date, however, state-of-the-art historical biogeographical approaches have not been applied to this problem. In this investigation, multiple chloroplast gene (rbcL, atpA, atpB, and matK) sequences for ferns in the family Onocleaceae Pic. Serm. are drawn from GenBank, including those from the recently sequenced chloroplast genome of the East Asian fern O. sensibilis L. var. interrupta Maxim., and used to create molecular phylogenies using Bayesian (BEAUTi and BEAST) techniques. Using contemporary approaches for relaxed molecular clock divergence time estimation with fossil calibration, divergence time estimates for East Asian and eastern North American populations in the Onoclea sensibilis species complex are consistently reconstructed as the Pliocene (ca. 5 – 3.4 Ma), and the best-fitting historical biogeographic model is a DIVA-like (exclusively vicariant) model using BIOGEOBEARS in RASP4, with a low probability of peripatry. Accordingly, these molecular and fossil data appear congruent with the pattern observed among angiosperms, despite the propensity for long-distance dispersal of wind-blown spores in pteridophytes. The lack of evidence for peripatry in this lineage may be related to the presence of short-lived green spores in onocleoid ferns; however, this hypothesis requires further investigation.

Walking the tightrope of real exchange rate policy for development: the roles of targets, instruments, and saving rates

Real exchange rate policy can potentially be utilized to target the trade balance and/or development through capital accumulation. However, the presence of distributional conflict and the tradeoff between current and future trade imbalances complicates matters. For example, steps that address development/external account issues may influence income distribution in socially unacceptable ways. Comparative studies of the East Asian and Latin American development experiences serve to highlight this issue. I show that policy assignment matters for dynamic stability, that is, the simultaneous achievability of multiple targets. Moreover, the relative saving behavior of different functional income groups influences dynamic behavior. The analysis sheds light on why real exchange rate policy may often be infeasible, even if desirable from a developmental perspective.

Generation and Characterization of Monoclonal Antibody Against Porcine Epidemic Diarrhea Virus Nonstructural Protein 13.

Porcine epidemic diarrhea virus (PEDV) is an enteric swine coronavirus. Recent PEDV eruption in East Asian and North American countries made it notorious and caused huge economic losses to the porcine industry. Nonstructural protein 13 (nsp13) is a nucleic acid helicase/NTPase that plays a critical role in viral gene transcription and viral replication. To investigate the function of nsp13 in the context of PEDV infection, here, PEDV nsp13 gene was amplified and cloned into pET28a/pET30a/pGEX-6P-1 expression vectors. The recombinant his-tagged nsp13 and GST-tagged nsp13 were expressed. Purified his-tagged nsp13 from pET28a-nsp13 vectors was chosen for immunization. Three monoclonal antibodies (mAbs) named 5A9, 5C7, and 5G7 were identified from the hybridoma cells, and were characterized by Western blot analysis and immunofluorescent assay, which demonstrated high specificity of these three mAbs with pCAGGS-HA-nsp13. All three mAbs belong to IgG1+ kappa subclass. However, only mAb 5A9 could effectively and specifically recognize PEDV expressing nsp13. Furthermore, the generated antibody against nsp13 could be applied to investigate nsp13 function during PEDV replication.

Analysis of latent T-cell epitopes in Epstein-Barr virus isolated from extranodal nasal-type natural killer/T-cell lymphoma in Taiwanese population

Extranodal nasal-type natural killer (NK)/T-cell lymphoma (NKTCL) is an aggressive lymphoma that is prevalent among East Asian and South American populations. Although Epstein-Barr virus (EBV) is commonly detected in NKTCL, there are limited studies that have analyzed the EBV genomic variations in NKTCL. In this study, 8 EBV latent genes were analyzed using targeted gene sequencing in 23 formalin-fixed paraffin-embedded tissues derived from 18 patients with NKTCL. Five cases with paired samples were comparatively analyzed. The consistency of EBV sequencing data between tissue samples was high (96.3%–98.7%), whereas that of variant calling among the tissue samples and plasma samples (74.3%–79.2%) was low. The highest densities of non-synonymous variants were detected in the EBNA3B gene. Among the 74 known T-cell epitopes, 363 non-synonymous variants were identified in 32 (43.2%) epitopes. Additionally, the AVFDRKSDAK (A1S/P and V2F/M/L) and YHLIVDTDSL (I4L and L10R/V/G/H) epitopes were associated with 5 patterns of amino acid changes in EBNA3B and EBNA-2, respectively. The frequency of variation in the human leukocyte antigen (HLA)-restricted epitopes with corresponding HLA types common among Taiwanese population was significantly low (P = 0.011), whereas that in anchor residues was significantly high (P = 0.012). In conclusion, this study demonstrated the genomic diversity of EBV in NKTCL and its correlation with the HLA-restricted epitope variations in Taiwanese population. The findings of this study provide useful insights for the development of novel therapeutic strategies for NKTCL.

Dry Port: A Review on Concept, Classification, Functionalities and Technological Processes

The purpose of this article is to offer a literature review on the development and classification of inland terminals, later defined as “dry ports”. The aim of the paper is to analyze the extant literature on dry ports focusing on their concept, classification, function and technological processes. The review offers an updated structured approach to what is currently defined as a dry port. To this end, a structured keyword search in major electronic databases has been conducted to find related material. As there are many different names indicating dry ports in European, South East Asian and North American countries, the following keywords were used: “dry port”, “inland terminal”, “freight village” and “interporto/i”. The search was conducted in respect of the article title and text, abstract and keywords. The results show that there is no unanimous consensus concerning cataloguing of terrestrial nodal facilities serving port gateways. “Dry ports” have emerged as fundamental elements of the integration between the sea “system” and the land network. The increased interest in the genesis and development of dry ports has been accompanied by an abundant contribution of the scientific community, originating a thriving literature, which, however, does not find a common denominator.

Alzheimer's Disease Screening Tools for Asian Americans: A Scoping Review.

With growing numbers of aging Asian Americans, Native Hawaiians, and Pacific Islanders (AANHPIs), the availability of culturally and linguistically appropriate screening tools for Alzheimer's disease and its related dementias (ADRD) is needed. The aim of this scoping review is to summarize the tools that have been adapted for and validated among the AANHPI population. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocol for systematic reviews, six databases were searched for peer-reviewed articles describing ADRD among AANHPIs. Among 1,477 articles screened for inclusion, 15 articles were included in the final analysis. Results showed a paucity of studies that psychometrically validate tools among this population. Furthermore, studies that culturally adapted and/or translated existing tools mostly targeted East Asian American populations. Our review provides a first step in mapping the extant literature on ADRD screening tools for this underresearched population and will serve as a guide for future research, policy, and intervention.

Neighborhood social cohesion and disease prevention in Asian immigrant populations

In the United States (US), chronic disease risk factors are highly prevalent among Asian immigrant communities, who also exhibit low health screening rates. Perceived neighborhood social cohesion (NSC) has been associated with preventive healthcare use in the general US population, although it remains unexplored among Asian Americans (AAs). The aim of this study is to evaluate the association between NSC and preventive screening for hypertension, high cholesterol, diabetes, and depression among East, South and Southeast Asian American (EAA, SAA, SEAA) communities in New York City (NYC) using cross-sectional, locally collected data from 2013 to 2016. NSC was assessed using a 4-question scale to create an additive score between 4 and 16 and was analyzed both as a continuous and categorial variable (High, Medium, and Low tertiles). Recent screening was defined as a check-up within the last year for each of the included health conditions. A one-unit increase in NSC score was associated with increased odds of recent screening for high cholesterol in EAAs (AOR = 1.09, 95%CI:1.00–1.20); for high cholesterol, diabetes, and depression in SAAs (AOR = 1.08, 95%CI:1.00–1.20; AOR = 1.07, 95%CI:1.00–1.15; AOR = 1.15, 95%CI:1.06–1.25); and with high cholesterol among SEAAs (AOR = 1.12, 95%CI:1.00–1.25). Overall, NSC was an important facilitator for preventive screening behaviors for specific conditions in different groups, though was consistently associated with screening for high cholesterol in our sample. Enhancing NSC through family and community-based programming may be one strategy to encourage screening for preventive behaviors, though more research is needed to elucidate a precise mechanism.

East Asian American Parents of Children with Autism: a Scoping Review

The number of Asian American children with autism is steadily increasing. Previous research reports that parents of children with autism experience higher stress compared with parents of typically developing children. Immigrant families of children with autism are particularly vulnerable to poor access to healthcare, social services, information, and other supports. This scoping review examined the current literature focused on East Asian American families raising children with autism, with an emphasis on study characteristics and overall findings. We identified nine articles that reported on the caregiving experiences of this population. Our review revealed that extant studies focused on East Asian families used limited research methods and small samples. The studies we reviewed found variations in the ways in which East Asian parents understand autism, navigate services, access available community and family supports, and experience stress.

A link triggered by tropical Pacific sea surface temperature between the East Asian and North American summer monsoon marginal zone precipitation at various time scales

Abstract The ocean plays a crucial role in the global and regional climate. Many regional monsoon climates are interlinked by ocean currents and sea surface temperature (SST). The climate link between the East Asian and North American continent has been proposed and verified by many studies. A widely accepted hypothesis is that the precipitation of the monsoon marginal zone is strongly affected by the monsoon intensity. However, our rigorous test does not support a causal relation between precipitation in the monsoon marginal zone and summer monsoon intensity. Here we present systematic analysis with modern observation and simulation datasets, the Holocene paleoclimatic records and simulations from the East Asian summer monsoon (EASM) and North American summer monsoon (NASM) marginal zone to verify the relation between precipitation in the monsoon marginal zone and the ocean. Our results suggest that precipitation of the EASM and NASM marginal zone is affected by the Pacific SST and the El Nino-Southern Oscillation (ENSO) rather than the monsoon intensity. In particular, the impacts of SST lead to synchronous variations in the EASM and NASM marginal zone. With the global warming and increasing ENSO events, precipitation in the monsoon marginal zone, which locates in semi-arid regions, would reduce, resulting in an expansion of the semi-arid region.

Nowe surowce roślinne w Farmakopei Europejskiej. Cz. 8. Camellia sinensis (L.) Kuntze (Herbata chińska) – źródło surowca katechinowo-alkaloidowego

In Supplement 9.4 to the European Pharmacopoeia, one of the newest pharmacopoeial documents, there are eight new monographs raw materials of plant origin. They are derived from both East Asian and South American species. The first two parts of the series of articles are devoted to the characteristics of plants, which are a source of saponin raw materials: Bupleurum sp. and Platycodon grandiflorus. The next parts: 3, 4 and 5 describe the East Asian species: Ligusticum huanxiong, Houttuynia cordata and Paeonia x suffruticosa, which provide raw materials rich in volatile oil, flavonoids and terpenoid-phenolic compounds, respectively. Two South American alkaloid-rich species: Paullinia cupana and Ilex paraguariensis are presented in parts 6 and 7. Part 8 characterizes Camellia sinensis, as a source of Camelliae sinensis non fermentata folia. This raw material is a rich source of polyphenols: catechins (e.g. epigallocatechin, gallocatechin and epicatechin gallates), purine alkaloids (mostly caffeine) and a unique non-protein amino acid, theanine. It has been proven that the raw material possesses among others a number of medicinal properties, including antioxidant, anticancer, antimicrobial, hypolipemic, hypocholesterolemic, hepatoprotective and antidiabetic properties. The aim of the present article is to draw attention of pharmaceutical and medical community to the numerous therapeutic, health-promoting and cosmetic values of this raw material proven by professional scientific studies.

ACE2 coding variants in different populations and their potential impact on SARS-CoV-2 binding affinity

The susceptibility of different populations to SARS-CoV-2 infection is not yet understood. Here, we combined ACE2 coding variants' analysis in different populations and computational chemistry calculations to probe the effects on SARS-CoV-2/ACE2 interaction. ACE2-K26R; which is most frequent in Ashkenazi Jewish population decreased the SARS-CoV-2/ACE2 electrostatic attraction. On the contrary, ACE2-I468V, R219C, K341R, D206G, G211R increased the electrostatic attraction; ordered by binding strength from weakest to strongest. The aforementioned variants are most frequent in East Asian, South Asian, African and African American, European, European and South Asian populations, respectively.

Functional prediction and comparative population analysis of variants in genes for proteases and innate immunity related to SARS-CoV-2 infection

New coronavirus SARS-CoV-2 is capable to infect humans and cause a novel disease COVID-19. Aiming to understand a host genetic component of COVID-19, we focused on variants in genes encoding proteases and genes involved in innate immunity that could be important for susceptibility and resistance to SARS-CoV-2 infection.Analysis of sequence data of coding regions of FURIN, PLG, PRSS1, TMPRSS11a, MBL2 and OAS1 genes in 143 unrelated individuals from Serbian population identified 22 variants with potential functional effect. In silico analyses (PolyPhen-2, SIFT, MutPred2 and Swiss-Pdb Viewer) predicted that 10 variants could impact the structure and/or function of proteins. These protein-altering variants (p.Gly146Ser in FURIN; p.Arg261His and p.Ala494Val in PLG; p.Asn54Lys in PRSS1; p.Arg52Cys, p.Gly54Asp and p.Gly57Glu in MBL2; p.Arg47Gln, p.Ile99Val and p.Arg130His in OAS1) may have predictive value for inter-individual differences in the response to the SARS-CoV-2 infection.Next, we performed comparative population analysis for the same variants using extracted data from the 1000 Genomes project. Population genetic variability was assessed using delta MAF and Fst statistics. Our study pointed to 7 variants in PLG, TMPRSS11a, MBL2 and OAS1 genes with noticeable divergence in allelic frequencies between populations worldwide. Three of them, all in MBL2 gene, were predicted to be damaging, making them the most promising population-specific markers related to SARS-CoV-2 infection.Comparing allelic frequencies between Serbian and other populations, we found that the highest level of genetic divergence related to selected loci was observed with African, followed by East Asian, Central and South American and South Asian populations. When compared with European populations, the highest divergence was observed with Italian population.In conclusion, we identified 4 variants in genes encoding proteases (FURIN, PLG and PRSS1) and 6 in genes involved in the innate immunity (MBL2 and OAS1) that might be relevant for the host response to SARS-CoV-2 infection.

WAGE INEQUALITY, UNEMPLOYMENT, AND EXPORT-ORIENTED POLICY

This paper presents a simple model to address why openness to trade increases the dispersion in wages, unemployment, and capital intensity. However, the dispersion is stronger for developing countries. We argue that the export-oriented policy that most developing countries have widely adopted in recent decades, amplifies the dispersion in these countries. This paper also helps explain the conflicting evidence between two groups of developing countries: East Asian and Latin American. In comparison to the latter, the former has a track record since the 1960s of a miraculous performance in narrowing wage inequality and unemployment by practicing export-oriented policies.

Plastome Phylogenomic and Biogeographical Study on Thuja (Cupressaceae).

Investigating the biogeographical disjunction of East Asian and North American flora is key to understanding the formation and dynamics of biodiversity in the Northern Hemisphere. The small Cupressaceae genus Thuja, comprising five species, exhibits a typical disjunct distribution in East Asia and North America. Owing to obscure relationships, the biogeographical history of the genus remains controversial. Here, complete plastomes were employed to investigate the plastome evolution, phylogenetic relationships, and biogeographic history of Thuja. All plastomes of Thuja share the same gene content arranged in the same order. The loss of an IR was evident in all Thuja plastomes, and the B-arrangement as previously recognized was detected. Phylogenomic analyses resolved two sister pairs, T. standishii-T. koraiensis and T. occidentalis-T. sutchuenensis, with T. plicata sister to T. occidentalis-T. sutchuenensis. Molecular dating and biogeographic results suggest the diversification of Thuja occurred in the Middle Miocene, and the ancestral area of extant species was located in northern East Asia. Incorporating the fossil record, we inferred that Thuja likely originated from the high-latitude areas of North America in the Paleocene with a second diversification center in northern East Asia. The current geographical distribution of Thuja was likely shaped by dispersal events attributed to the Bering Land Bridge in the Miocene and subsequent vicariance events accompanying climate cooling. The potential effect of extinction may have profound influence on the biogeographical history of Thuja.

DRD4 polymorphisms modulate reward positivity and P3a in a gambling task: Exploring a genetic basis for cultural learning.

Prior work shows that people respond more plastically to environmental influences, including cultural influences, if they carry the 7 or 2-repeat (7/2R) allelic variant of the dopamine D4 receptor gene (DRD4). The 7/2R carriers are thus more likely to endorse the norms and values of their culture. So far, however, mechanisms underlying this moderation of cultural acquisition by DRD4 are unclear. To address this gap in knowledge, we tested the hypothesis that DRD4 modulates the processing of reward cues existing in the environment. About 72 young adults, preselected for their DRD4 status, performed a gambling task, while the electroencephalogram was recorded. Principal components of event-related potentials aligned to the Reward-Positivity (associated with bottom-up processing of reward prediction errors) and frontal-P3 (associated with top-down attention) were both significantly more positive following gains than following losses. As predicted, the gain-loss differences were significantly larger for 7/2R carriers than for noncarriers. Also, as predicted, the cultural backgrounds of the participants (East Asian vs. European American) did not moderate the effects of DRD4. Our findings suggest that the 7/2R variant of DRD4 enhances (a) the detection of reward prediction errors and (b) controlled attention that updates the context for the reward, thereby suggesting one possible mechanism underlying the DRD4 × Culture interactions.

Nowe surowce roślinne w Farmakopei Europejskiej. Część 7. Ilex paraguariensis A. St.-Hil. (ostrokrzew paragwajski) – źródło nowego surowca alkaloidowego

One of the newest pharmacopoeial documents, the European Pharmacopoeia Supplement 9.4 contains monographs of several new raw materials of plant origin, long used in East Asian and South American medicine. Part 1 and 2 presents characteristics of the plant species providing new saponin raw materials (Bupleurum sp., Platycodon grandiflorus). Part 3 describes Ligusticum chuanxiong, which is the source of a new raw material rich in volatile oil. Part 4 is dedicated to Houttuynia cordata and Part 5 to Paeonia x suffruticosa. These plants supply a new flavonoid and terpenoid-phenolic raw materials. Part 6 is focused on characteristics of Paullinia cupana (a source of alkaloid raw material). On the other hand, Part 7 presents botanical, ecological and chemical characteristics of Ilex paraguariensis (yerba-mate). Mate folium, a raw material derived from this species has long been used by indigenous peoples of Argentine, Brazil, Paraguay and Uruguay in the form of infusion as a traditional stimulating drink. This raw material is particularly rich in caffeine and theobromine, chlorogenic acid, dicaffeoylquinic acids and catechins. The backbone of the article is made up of a review of therapeutic applications of this raw material confirmed by scientific studies. Mate folium appears as a kind of “panacea” efficient in lifestyle diseases currently threatening the mankind. The aim of the article is to popularize professional knowledge of this species, to date insufficiently known in Poland, in pharmaceutical and medical community.

Fostering Bicultural Skills among East Asian American Youth: An Initial Investigation of the East West Connection Program

We examined the efficacy of the East West Connection (EWC) program for East Asian American youth (N = 51) using a quasi-experimental design. Participants were recruited from several churches. While our study showed that the participation in EWC did not significantly increase bicultural competence, the regression analyses did reveal that bicultural competence was a significant predictor of well-being. We discuss the implications of the results for future intervention development and provide recommendations for future research.

Determinants of Multifactor Productivity: The Cases of the Main Latin American and Successful Asian Economies (1960-2015)

The objective of this paper was to explain the greater or lesser growth rate of total factor productivity, TFP, in the main East Asian and Latin American economies between 1960 and 2015. We found econometric evidence favorable to this hypothesis: the increase in public consumption expenditures, given the evolution of other factors, reduces the TFP. Other results of the econometric exercise, those that are related to hypothetical positive effects of public investment and imports on TFP were not as robust or as reliable as we would have expected.

Addressing the challenge of food-borne trematodiases in East Asian and South American regions

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