Ease Of Selection Research Articles

Study for Some Body Weight and Egg Traits in Domyati and Khaki-Campbell Ducks

The duck industry makes an important contribution to the availability of animal protein sources in Egypt, little known about the genetic parameters, particularly the heritability and genetic correlations of body weight and egg production in ducks. Body weight is the most essential feature for genetic improvement due to its ease of selection, high heredity, and large impact on meat production costs. The target of this study was to evaluate and explain genetic parameters such as the heritability, the genetic and phenotypic correlations, and sire breeding value in Domyati (local) and Khaki-Campbell (foreign) ducks in order to improve body weight and egg traits. A total of 160 (80 Domyati and 80 Khaki-Campbell ducks utilized to measure body weight at 16 and 20 weeks g), as well as 7000 eggs (2500 Domyati and 4500 Khaki-Campbell) to measure egg traits (the egg number, egg weight, and egg mass are all measured throughout the first 90 days of laying). In Domyati and Khaki-Campbell ducks, the heritability estimated for body weight was moderate to high, ranged from 0.35 to 0.40, and 0.21 to 0.30 for egg production. The genetic correlations among body weight and egg traits were all positive and had high values, also among BW16 and BW20 were stronger (0.90); (0.99). So the genetic improvement in BW16 could be followed by an increase in BW20 weeks. It concluded that, the relatively high value of genetic heritability for body weights and egg traits in Domyati and Khaki-Campbell ducks, indicates that it is possible to genetically increase body weight and egg traits through selection and subsequently inbreeding to divide the herd into groups that are selected among themselves to keep their sons.

Phenotypic Characteristics of Native Genotypes Belonging to Koeleria genus

Some plant species belonging to the Koeleria genus have many features that make it an ideal grass with low maintenance costs. Their slow growth habit, tolerance to drought and extreme environmental conditions, and their wide natural distribution make them strong candidates for development as grass and forage. In this study, observations and measurements were carried out in 2019-2020 to determine phenotypic characteristics in 53 genotypes selected according to their superior characteristics from Koeleria genotypes collected from the natural flora of Turkey. In Koeleria genotypes; Quality (1-9 scale), seasonal color change (1-9 scale), leaf texture (1-9 scale), density (1-9 scale), plant height (cm), leaf width (cm), leaf length ( cm), leaf area (cm²), growing type in autumn (1-9 scale), plant diameter (cm), spring regrowth time (1-9 scale), panicle formation tendency (1-9 scale), panicle height (cm ), last internode length (cm), seed yield (g) mean values were 6.90, 4.13, 4.01, 4.73, 52.11, 0.21, 16.15, 3.42, 3.60, 46.33, 6.54, 5.37, 7.69, 16.88, 4.7, respectively. Correlation analysis table was created among the phenotypic traits examined and it was found that the traits were significantly effective on each other. Positive correlations between some of these will allow for multi-featured selection in variety improvement. In addition, in the dendrogram created as a result of the cluster analysis, the genotypes are divided into 2 main and 2 subgroups in each main group. This analysis provides ease of selection according to the characteristics focused on these groups for synthetic variety breeding and future breeding studies. As a result of this study, the superior Koeleria genotypes that can be used in breeding studies will be determined.

Implementation Challenges With Evidence-Based Practice Within Inpatient Rehabilitation to Incorporate Task-Specific Training

Abstract Date Presented Accepted for AOTA INSPIRE 2021 but unable to be presented due to online event limitations. The study objective was to examine the implementation barriers and facilitators after a year of training and use of a task-specific training manual in inpatient rehabilitation. The Consolidated Framework for Implementation Research provided structure for the survey of OTs. Stakeholders reported confidence in evidence incorporation, and the barriers related to ease of selection and use of this intervention need to be addressed. Primary Author and Speaker: Eron Bozec Additional Authors and Speakers: Namrata Grampurohit Contributing Authors: Namrata Grampurohit, Jaime Gorska

Reagentless and Ultrasensitive Electrochemical Dnazyme Based Sensing Platform for Clinical Detection of Uropathogenic Escherichia coli

Urinary tract infections (UTIs) are one of the most ubiquitous bacterial infection diseases affecting half of the global population at least once in their lifetime. Given the occurrence of the false positive result in dipstics and the time-consuming identification of bacteria using culturing method to detect UroPathogenic Escherichia coli (UPEC), there is the need for a point-of-care diagnostic platform that is reliable, sensitive, specific, cost-effective, fast and easy to use. With the advent electrochemical methods coupled with the specific biorecognition elements, the detection of any biomarker has become possible with one touch. Among many biorecognition molecules, DNAzymes are gaining attention due to their robustness, stability, specificity, and the ease of selection without prior knowledge of the biomarker. Their structure switching and functionality (RNA cleaving) can be utilized with redox active species (like methylene blue) to design highly sensitive and selective electrochemical assays for pathogen detection. In this work, we have developed a dual signaling electrochemical DNAzyme based platform for specific and sensitive detection of Escherichia coli (E.coli) in UTI patient urine samples. The two on-chip integrated working electrodes named as release channel and capture channel are integrated with DNAzyme-redox barcode and DNA probe respectively. In the presence of the bacterial target the DNAzyme catalyzes the cleavage of the redox DNA barcode in the release channel, which is subsequently translated into a redox signal upon hybridization with the probe on the capture channel. The differential signal generated by the two channels in the sensor demonstrated the sensitive detection of 10 CFU for E.coli in buffer and urine. Additionally, the sensor specifically detected E. coli in the presence of a panel of other gram positive and negative bacteria. Clinical validation of the assay was performed using 45 E.coli+ and E.coli- patient urines delineating a clinical sensitivity of 100% and specificity of 78% in a 30 minute testing time. This cost effective, reagent-free, time-efficient, and amplification-free electrochemical assay is suitable for detecting a wide range of pathogens, also alleviates the additional knowledge requirement of the biomarker as compared to other protein and aptamer-based assay. With the advancement in the chip fabrication and microfluidics, this assay can also be multiplexed with other pathogen for simultaneous detection of multiple pathogens in a single urine sample.

Fabrication of poly-sulfosalicylic acid film decorated pure carbon fiber as electrochemical sensing platform for detection of theophylline

In this work, we integrated the superiority of good conductivity, large surface area of carbon fibers and the catalytic property, good biocompatibility of polymer sulfosalicylic acid to construct a novel electrochemical sensor to detect theophylline in drug analysis. The morphology of nanocomposite was characterized by scanning electron microscopy (SEM). The polymerization between monomers was observed by Fourier transform infrared spectroscopy (FTIR). The composite between carbon material and polymer was verified by Raman spectrum. Under the optimal experimental conditions, the concentration of theophylline (0.6∼137 μM) and the peak current value revealed a good linear relationship and the limit of detection as low as 0.2 μM. In addition, the proposed sensor exhibits repeatability, stability and ease of selectivity.

Fabrication of Poly-Sulfosalicylic Acid Film Decorated Pure Carbon Fiber As Electrochemical Sensing Platform for Detection of Theophylline

Theophylline (1,3-dimethylxanthine, TP) is a xanthine alkaloid that stimulates breathing system. It is widely used as a bronchodilator drug for the treatment of various asthma diseases. Determining the concentration of theophylline is important in medical affairs through simple, rapid, accurate and inexpensive methods. Electrochemical technology has got development due to its high sensitivity, stability, simplicity, low cost, ease of use, and in-situ analysis.In this work, we combined the advantage of good conductivity, large surface area of carbon fibers and the catalytic property, good biocompatibility of polymer sulfosalicylic acid to construct a novel electrochemical sensor to detect theophylline in drug analysis. The morphology of nanocomposite was characterized by electron microscopy (SEM). The polymerization between monomers was observed by Fourier transform infrared spectroscopy (FTIR). The composite between carbon material and polymer was verified by Raman spectrum. Under the optimal experimental conditions, the concentration of theophylline (0.6 ~ 137μM) and the peak current value showed a good linear relationship and the limit of detection as low as 0.2μM. In addition, the proposed sensor exhibits repeatability, stability and ease of selectivity.

Ultrasensitive Electrochemical Dnazyme Based Sensing Platform for Clinical Detection of Uropathogenic Escherichia coli

Urinary tract infections (UTIs) are one of the most ubiquitous bacterial infection diseases affecting half of the global population at least once in their lifetime. Given the occurrence of the false positive result in Dipstics and the time-consuming identification of bacteria using culturing method to detect UroPathogenic Escherichia coli (UPEC), there is the need for a point-of-care diagnostic platform that is reliable, sensitive, specific, cost-effective, fast and easy to use. With the advent electrochemical methods coupled with the specific biorecognition elements, the detection of any biomarker has become possible with one touch. Among many biorecognition molecules, DNAzymes are gaining attention due to their robustness, stability, specificity, and the ease of selection without prior knowledge of the biomarker. Their structure switching and functionality (RNA cleaving) can be utilized with redox active species (like methylene blue) to design highly sensitive and selective electrochemical assays for pathogen detection.In this work, we have developed a dual signaling electrochemical DNAzyme based platform for specific and sensitive detection of Escherichia coli (E.coli) in UTI patient urine samples. The two on-chip working electrodes named as release channel and capture channel are integrated with DNAzyme-redox barcode and DNA probe respectively. In the presence of the bacterial target the DNAzyme catalyzes the cleavage of the redox DNA barcode in the release channel, which is subsequently translated into a redox signal upon hybridization with the probe on the capture channel. The differential signal generated by the two channels in the sensor demonstrated the sensitive detection of 10 CFU for E.coli in buffer and urine. Additionally, the sensor specifically detected E. coli in the presence of a panel of other gram positive and negative bacteria. Clinical validation of the assay was performed using 40 E.coli+ and E.coli- patient urine delineating a clinical sensitivity of 100% and specificity of 78% in a 30 minute testing time. This cost effective, reagent-free, time-efficient, and amplification-free electrochemical assay is suitable for detecting a wide range of pathogens, also alleviates the additional knowledge requirement of the biomarker as compared to other protein and aptamer-based assay. With the advancement in the chip fabrication and microfluidics, this assay can also be multiplexed with other pathogen for simultaneous detection of multiple pathogens in a single urine sample.

The Diminishing Returns for Longer Healthcare Provider Video Biographies: A Thin Slice Examination of Patient Decision-Making

ABSTRACT The consumer-centric nature of the current healthcare landscape in the United States is forcing healthcare organizations to innovate novel ways to help patients more efficiently and effectively select their healthcare providers to improve the patient experience. One way organizations are doing this is by providing introductory videos of their providers online to help patients informatively shop for new providers. However, producing videos – especially lengthy ones – for all providers within an organization can be both time consuming and expensive, with costs inevitably trickling down to patients. Framed by the concepts of thin slicing from the psychological sciences, and uncertainty reduction from the communication sciences, the primary purpose of this 12-condition online experiment (n = 1,310) was to determine the optimal length of healthcare providers’ introductory biographical videos in-terms of patients’ ease of selection, and the impact that video length can have on patients’ provider perceptions. Results reveal that introductory videos of around 46-seconds, as well as shorter videos with supplemental text, provide similar results on dependent variables such as selection ease, patient satisfaction, and trust, as do longer videos. Additional recommendations for applying these findings within healthcare organizations are discussed.

Abstract TP44: Trans-Radial Approach in Mechanical Thrombectomy in Anterior Circulation Large Vessel Stroke; Two Center Experience

Background: Trans-femoral access (TFA) is used conventionally for mechanical thrombectomy (MT) for large vessel occlusion (LVO) stroke. Trans-radial access (TRA) is not only an alternative access route when TFA is not successful but has been shown to significant advantages over TFA in cardiac interventions in large prospective studies. In LVO stroke also, TRA may have several advantages over TFA, including greater technical ease of selection of great vessels at the arch in Type 2 & 3 arches in older stroke patients, avoidance of aorto-illiac disease and less frequency of access complications. We assessed the technical feasibility and safety of TRA for MT in Anterior Circulation LVO in 2 large tertiary care hospitals. Methods: This is a retrospective two-center study of patients undergoing MT for anterior LVO via a TRA from January 2015 through June 2017. From the institutional database of patients receiving MT we identified consecutive patients in whom a TRA was performed for MT for anterior circulation Large vessel occlusion. A 6F guide sheath and 6F Guide-catheter system introduced through the radial artery with or without balloon at distal tip was used for the MT procedure with a standard microcatheter MT technique. We measured workflow metrics, complications and feasibility and safety in this cohort. Results: 27 out of 390 (6.9%) MT patients underwent TRA MT for anterior LVO during the study period. There were 8 males (29.6%) and 19 females (70%). There were 9 patients (33.3%) in whom a TFA was attempted initially and had to be converted to a TRA due to inability to catheterize the great vessels via TFA. In rest of the patients, TRA was the first line approach. The mean age of patients was 80 ±12, females 84.5±9.6 years, males 74.1±16.2 years. Median time from access to reperfusion was 60 minutes (IQR 37 to 94). 22 (81.4%) had excellent rates of revascularization (TICI 2b, 3). Symptomatic ICH was observed in 3 patients (11.1%). There were no access related complications were noted in any of the patients. Conclusion: TRA for MT in patients with anterior circulation LVOs is feasible, safe and achieves rapid reperfusion similar to TFA for MT. Apart from being an alternative when TFA is not feasible, it should be studied further as a first line approach for MT in LVO stroke.

Design Heuristics for Effective Smartphone Questionnaires

Design principles for survey questionnaires viewed on desktop and laptop computers are increasingly being seen as inadequate for the design of questionnaires viewed on smartphones. Insights gained from empirical research can help those conducting mobile surveys to improve their questionnaires. This article reports on a systematic literature review of research presented or published between 2007 and 2016 that evaluated the effect of smartphone questionnaire design features on indicators of response quality. The evidence suggests that survey designers should make efforts to “optimize” their questionnaires to make them easier to complete on smartphones, fit question content to the width of smartphone screens to prevent horizontal scrolling, and choose simpler types of questions (single-choice questions, multiple-choice questions, text-entry boxes) over more complicated types of questions (large grids, drop boxes, slider questions). Based on these results, we identify design heuristics, or general principles, for creating effective smartphone questionnaires. We distinguish between five of them: readability, ease of selection, visibility across the page, simplicity of design elements, and predictability across devices. They provide an initial framework by which to evaluate smartphone questionnaires, though empirical testing and further refinement of the heuristics is necessary.

ДО МЕТОДУ ОТРИМАННЯ МЕЗЕНХІМАЛЬНИХ СТОВБУРОВИХ КЛІТИН ВЕЛИКОЇ РОГАТОЇ ХУДОБИ

Prospects receipt and use of multipotent mesenchymal stem cells of an adult organism, determined by their basic properties and characteristics. Diploid cell cultures derived from mammalian tissues and organs, preserve their species and tissue specificity, but have a limited period of proliferation because of aging. Therefore, the use of mesenchymal stem cells is important. The ease of selection and availability of biological material makes them today to date, the most promising cell system. Studying the properties of stem cells in veterinary medicine has its own characteristics. They are in particular, taking into account specific characteristics of the cell material, which is important in practical veterinary regenerative therapy. There is a significant amount of minutes of culturing mesenchymal stem cells, but none of the authors do not focuses on the expression of biological properties of these cells by culturing. In the study of the biological properties of mesenchymal stem cells, there are many mysteries and the unknown, but today conducted preclinical and clinical studies for the introduction of these cells to correct the damaged organs and tissues of an animal body. Thus, the study and knowledge of the underlying mechanisms that form the specific features of the biological properties of stem cells in cattle is important and urgent to develop scientifically based methods of use of mesenchymal stem cells in regenerative therapy of breast diseases in cows. Because of this work was perfected method of cultivation and crop deep freeze multipotent mesenchymal stem cells isolated from bone marrow of cattle. In studies we used mesenchymal stem cells derived from bone marrow of healthy donor cows. For bone marrow, we used our improved method. Comparative analysis of the efficiency of the allocation of mesenchymal stem cells using heparin or sodium citrate indicates no significant differences and can be used both drugs. When cultured mesenchymal stem cells by cell separation density gradient fikola is more efficient, while the second method also can be. The proposed method of obtaining and culturing multipotent mesenchymal stem cells isolated from bone marrow of cattle makes it possible to obtain stem cells with high biological activity.

Performance based Seismic Analysis on RCC Framed Elevated Circular Tanks with Flat and Domical Bases

Objectives: This paper replicates the comparative study of RCC staged circular water tanks with flat base and domical base of same capacity of 320m3 under seismic conditions. Method: Analysis is carried out for dynamic responses, with inclusive variables as height of staging, soil condition, damping factor, seismic zones adhering to IS 1893-2002. Layout of staging frame and circular tank is with accordance to IS 11682-1985 and IS 3370 accordingly. Series of analysis has been done using Staad pro v8i as a tool for Modal analysis and Response spectrum analysis adhering to IS 1893-2002, and response is depicted in the form of graphs for each zone. Findings: Graphs sketched for the governing design criteria i.e. Base Shear, for each height and type of tanks considered under the pertaining conditions for each zone perspicuously delivers the basic idea to the structural designer fellows for the ease of selection of water tank's configuration and height for optimum performance under seismic loading prior to designing in order to work out for a better and optimum design. Improvements: Different conditions has been incorporated as variables for precise and discreet graphs for each zone that is additive to the selection process of the structure.

E‐book acquisition features: attitude of Iranian information professionals

PurposeThis paper aims to consider the most important criteria of e‐book acquisition in academic libraries. The survey seeks these reasons on the basis of two aspects: e‐book features for users and for information professionals or libraries. Moreover, restrictions on e‐books acquisition are surveyed with regard to information professionals' attitude.Design/methodology/approachThe survey research method is used in this study with questionnaire and interview used for data gathering. The statistical population consisted of 60 information professionals working at state universities of metropolitan Tehran.FindingsThe results show that high storage capacity and multimedia capability are the most important features in acquiring e‐books on the basis of academic user needs. Also, the main criteria of acquiring e‐books from the professionals' point of view of fostering their interests are: occupying little space, ease of selection and simultaneous circulation. Moreover, this study shows that the main restrictions of e‐book acquisition are: hardware and software restrictions, the lack of a union catalog for Iranian e‐books, low level of user familiarity, hackers and even eye‐fatigue when reading.Originality/valueThis study takes the form of unique research that surveys the e‐book acquisition criteria in Iran on the basis of two aspects (user and library) with focus on information professionals' attitude, simultaneously. In addition, there has not been any research on e‐books in Iran, and this study is the first.

Tumor-Targeted Drug Delivery with Aptamers

Cancer is one of the leading causes of death around the world. Tumor-targeted drug delivery is one of the major areas in cancer research. Aptamers exhibit many desirable properties for tumor-targeted drug delivery, such as ease of selection and synthesis, high binding affinity and specificity, low immunogenicity, and versatile synthetic accessibility. Over the last several years, aptamers have quickly become a new class of targeting ligands for drug delivery applications. In this review, we will discuss in detail about aptamer-based delivery of chemotherapy drugs (e.g. doxorubicin, docetaxel, daunorubicin, and cisplatin), toxins (e.g. gelonin and various photodynamic therapy agents), and a variety of small interfering RNAs. Although the results are promising which warrants enthusiasm for aptamer-based drug delivery, tumor homing of aptamer-based conjugates after systemic injection has only been achieved in one report. Much remains to be done before aptamer-based drug delivery can reach clinical trials and eventually the day-to-day management of cancer patients. Therefore, future directions and challenges in aptamer-based drug delivery are also discussed.

Selection of resistance breaking strains of tomato spotted wilt tospovirus

Summary.In glasshouse tests, sap from plants infected with 15 different isolates of tomato spotted wilt tospovirus (TSWV) from three Australian states was inoculated to nine genotypes of tomato carrying TSWV resistance gene Sw‐5 or one of its alleles. A further two resistant tomato genotypes were inoculated with four isolates each. The normal response in resistant genotypes was development of necrotic local lesions in inoculated leaves without systemic invasion, but 22/752 plants also developed systemic reactions in addition to local hypersensitive ones. Using cultures from two of these systemically infected plants and following four cycles of subculture in TSWV resistant tomato plants, two isolates were obtained that gave susceptible type systemic reactions but no necrotic spots in inoculated leaves of resistant tomatoes. When these two isolates, DaWA‐1d and ToTAS‐1d, were maintained by repeated subculture for 10 successive cycles in Nicotiana glutinosa or a susceptible tomato genotype, they still induced susceptible type systemic reactions when inoculated to resistant tomato plants. They were therefore stable resistance breaking isolates as regards overcoming gene Sw‐5. When resistance‐breaking isolate DaWA‐1ld multiplied together with original isolate DaWA‐l in susceptible tomato, it was fully competitive with the original isolate. However, when DaWA‐ld and ToTAS‐ld were inoculated to TSWV resistant Lycopersicon peruvianum lines PI 128660R and PI 128660S and to TSWV resistant Capsicum chinense lines PI 152225, PI 159236 and AVRDC CO0943, they failed to overcome the resistance, producing only necrotic local lesions without systemic infection. Thus, although the ease of selection, stability and competitive ability of resistance breaking isolates of TSWV is cause for concern, L. peruvianum and C. chinense lines are available which are effective against them.The effectiveness of the resistance to TSWV in nine tomato genotypes was examined in a field experiment. Spread was substantial in the susceptible control genotype infecting 42% of plants. Resistance was ineffective in cv. Bronze Rebel, 26% of plants developing infection. In contrast, it held up well in the other eight resistant genotypes with only 1–3 or no plants of each becoming infected. Accumulated numbers of Thrips tabaci, Frankliniella occidentalis and F. schultzei were closely correlated with TSWV spread.

Chromosomal fragility associated with familial Alzheimer's disease.

To test whether chromosomal instability is associated with familial Alzheimer's disease, we examined breakage on X chromosomes of fibroblasts derived from patients with familial Alzheimer's disease, using gene cotransfer methodology. The X chromosome is a convenient target for analyzing DNA breakage because of its numerous markers and ease of selection in rodent-human hybrid cells. Patients with familial Alzheimer's disease, including the large Nova Scotia Alzheimer's kindred, show a significantly lower cotransfer of the X-linked glucose-6-phosphate dehydrogenase (G6PD) gene with the selected HPRT gene in hybrid cells, indicating breakage between the markers. Lower cotransfer of the more distant X-linked gene, MIC-2, was statistically significant in this kindred, but not in other patients with familial Alzheimer's disease. The distance between MIC2 and HPRT is sixfold to ninefold greater than that between HPRT and G6PD, suggesting that there may be a "hot spot" for breakage in the latter interval on the X chromosome of patients with familial Alzheimer's disease. The somatic cell hybrid model provides insights into underlying mechanisms for chromosomal breakage induced by the Alzheimer defect. A hypothesis implicating a candidate gene, C1-THF synthase, in the generation of chromosome instability in the pathogenesis of familial Alzheimer's disease, is presented.

Optical methods to measure shape and size.

A number of methods are available to quantify exterior size and shape of living and non-living objects. Relevant items for dentistry are the exterior of face and skull and the surface of dental casts. To the best of our knowledge, dentitions have not yet been measured in situ. The optical methods using incoherent light are mechanical sensing of casts, visual stereometry (on the subject or on stereophotographic pairs), moire techniques, and optical-sensor morphometry. It will be shown that the three latter systems in fact rely on the same physical principles, although they involve quite different technologies. On the other hand, coherent optical techniques, such as holography and contouring holographic interferometry, are presented. The basic principles of the different techniques are shown, and their main features in relation to applications to the dental object discussed. Main features include: resolving power, range, time needed for a measurement, requirements for the surface of the object, and ease of selection and collection of data. Examples of methods from the literature and from work by the author are given.

Small Computer System for Micrograph Analysis

Cost reduction and availability of specialized hardware for image processing have made it reasonable to purchase a stand-alone interactive work station for computer aided analysis of micrographs. Some features of such a system are: 1) Ease of selection of points of interest on the micrograph. A cursor can be quickly positioned and coordinates entered with a switch. 2) The image can be nondestructively zoomed to a higher magnification for closer examination and roaming (panning) can be done around the picture. 3) Contrast and brightness of the picture can be varied over a very large range by changing the display look-up tables. 4) Marking items of interest can be done by drawing circles, vectors or alphanumerics on an additional memory plane so that the picture data remains intact. 5) Color pictures can easily be produced. Since the human eye can detect many more colors than gray levels, often a color encoded micrograph reveals many features not readily apparent with a black and white display. Colors can be used to construct contour maps of objects of interest. 6) Publication quality prints can easily be produced by taking pictures with a standard camera of the T.V. monitor screen.