The loading rate of tectonic stress is not constant during long-term geotectonic activity and significantly affects the earthquake nucleation and fault rupture process. However, the mechanism underlying the loading rate effect is still unclear. In this study, we conducted a series of experiments to explore the effect of the loading rate on earthquake nucleation and stick–slip characteristics. Through lab experiments, faults were biaxially loaded at varying rates to produce a series of earthquakes (stick–slip events). Both shear strain and fault displacement were monitored during these events. The findings indicate a substantial effect of the loading rate on the recurrence interval and the shear stress drop of these stick–slip events, with the recurrence interval inversely proportional to the loading rate. The peak friction of the fault also decreases with the increasing loading rate. Notably, prior to the dynamic rupture of earthquakes, there exists a stable nucleation phase where slip occurs in a quasi-static manner. The critical nucleation length, or the distance required before the dynamic rupture, diminishes with both the loading rate and normal stress. A theoretical model is introduced to rationalize these observations. However, the rupture velocity of these lab-simulated earthquakes showed no significant correlation with the loading rate. Overall, this study enhanced our comprehension of earthquake nucleation and rupture dynamics in diverse tectonic settings.