The uplift of underground structures induced by soil liquefaction can damage underground structure systems. Numerical simulations have shown that uplift is positively correlated with the energy of horizontal input motion. However, the effects of vertical input motion on uplift have not been studied comprehensively in the past. Previous studies on the vertical motion concluded that the effects of vertical motion on uplift depend on the overall characteristics of earthquake motion. These motion characteristics have only been studied separately in previous studies. A comprehensive study to explore the interactions and overall effects of these characteristics on the uplift of underground structures is essential. In this study, the FLAC program with the PM4Sand model was used as a numerical tool to explore the effects of vertical input motion on the uplift of underground structures. The numerical model was calibrated using centrifuge test results, and 48 earthquake motions were selected as input motions to study the effects of the overall characteristics of earthquake motions on the uplift of underground structures. The simulation results show that the frequency content characteristics of horizontal and vertical motion are the major factors affecting the uplift magnitude and the responses of liquefiable soils. However, most simulation cases show that the inclusion of vertical motion causes a 10% difference in the tunnel uplift, compared to cases without vertical motion.