Early Stage Of Immersion Research Articles

The effects of Methanococcus maripaludis on the corrosion behavior of EH40 steel in seawater

The corrosion behavior of EH40 steel in seawater enriched with Methanococcus maripaludis was investigated through electrochemical methods and surface analysis techniques. The results revealed that the hydrogenotrophic M. maripaludis strain can utilize acetate as an alternative energy source. Corrosion of EH40 steel is initially inhibited, but prolonged exposure with the methanogen leads to an eventual corrosion propagation. During the early stage of immersion in M. maripaludis culture medium, the formation of a protective corrosion products film inhibits EH40 steel corrosion. The presence of M. maripaludis promotes both anodic and cathodic reactions of EH40 steel in the late stage of exposure. Surface analyses revealed that pitting corrosion is closely related to uneven distribution of M. maripaludis biofilm on EH40 steel surface.

Influence of the amount of intermetallics on the degradation of Mg-Nd alloys under physiological conditions

The influence of amount of intermetallics on the degradation of as-extruded Mg-Nd alloys with different contents of Nd was investigated via immersion testing in DMEM+10% FBS under cell culture conditions and subsequent microstructural characterizations. It is found that the presence of intermetallic particles Mg41Nd5 affects the corrosion of Mg-Nd alloys in two conflicting ways. One is their negative role that their existence enhances the micro-galvanic corrosion. Another is their positive role. Their existence favours the formation of a continuous and compact corrosion layer. At the early stage of immersion, their negative role predominated. The degradation rate of Mg-Nd alloys monotonously increases with increasing the amount of intermetallics. Mg-5Nd alloy with maximum amount of intermetallics suffered from the most severe corrosion. With the immersion proceeding (≥7 days), then the positive role of these intermetallic particles Mg41Nd5 could not be neglected. Owing to the interaction between their positive and negative roles, at the later stage of immersion the corrosion rate of Mg-Nd alloys first increases with increasing the content of Nd, then reaches to the maximum at 2 wt. % Nd. With a further increase of Nd content, a decrease in corrosion rate occurs. The main corrosion products on the surfaces of Mg-Nd alloys include carbonates, calcium-phosphate, neodymium oxide and/or neodymium hydroxide. They are amorphous at the early stage of immersion. With the immersion proceeding, they are transformed to crystalline. The existence of undegradable Mg41Nd5 particles in the corrosion layer can enhance the crystallization of such amorphous corrosion products.

Influences of aggressive ions in human plasma on the corrosion behavior of AZ80 magnesium alloy

Magnesium alloys can work as biomedical materials due to their Young's modules similar to that of bone. Nevertheless, in a human plasma, one of the major drawbacks of these materials is the low corrosion resistance. Here, AZ80 corrosion in the solutions containing chloride, bicarbonate, sulphate and hydrogen phosphate ions were investigated by a short-term immersion test and electrochemical techniques. The results showed that bicarbonate and hydrogen phosphate could retard corrosion rate, while chloride and sulphate accelerated corrosion rate. During the early immersion stage, the corrosion rate increased with the presence of bicarbonate. It was caused by the reaction of bicarbonate and hydroxide promoting the dissolution of magnesium and accelerating corrosion. In the later stage, the reduced corrosion rate was due to the formation of various protective films. The sample formed a new sparse porous MgSO4·5H2O compounds in the sulphate ion solution, which could not effectively prevent chloride ions from entering the matrix and thus accelerated the dissolution of magnesium. With the presence of hydrogen phosphate, magnesium phosphate with a much lower solubility was formed, preferentially precipitated on the surface and was not influenced by the chloride ions. The corrosion mechanisms of magnesium alloys in above ions were proposed.

A study of the in-vitro bioactivity, dissolution and antibacterial activity of larnite prepared by a novel sol–gel combustion method using sucrose as a fuel

This article presents the preparation and antibacterial activity of larnite bioceramics, an active calcium silicate ceramic material having the molecular formula Ca2SiO4. A stepwise sol–gel combustion approach was used to prepare the single phasic larnite. The synthesis of larnite was carried out with calcium nitrate tetrahydrate as a source of calcium which acts as an oxidizing agent and tetraethyl orthosilicate as a source of silicate which acts as a reducing agent (fuel). The powders thus prepared were calcined at temperatures ranging from 400 to 800°C. X-ray diffraction analysis was used to study the phase formation and determine the hydroxyapatite (HAP) formation during the bioactivity studies. The synthesized materials were also characterized by Fourier-transform infrared, scanning electron microscopy and energy dispersive X-ray techniques. Elemental ionic concentration of the Ca, P and Si in stimulated body fluid (SBF) solution was analysed by inductively coupled plasma-optical emission spectroscopy. The bone-like apatite formation ability of larnite scaffolds was investigated by immersing it in SBF. It was observed that larnite has the capability to deposit HAP within the early stage of immersion. The antimicrobial activity of the larnite was screened against nine clinical pathogens. The fabricated larnite compound was tested against both Gram-positive bacteria (Staphylococcus aureus and Enterococcus sp.) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Shigella sp., Proteus mirabilis, Salmonella sp. and Klebsiella pneumoniae) via the agar diffusion method. The obtained results suggested that the prepared larnite has a high antibacterial property against Gram-negative bacteria because of its thin peptidoglycan layer. From the overall results it is concluded that larnite could be a promising candidate for biomedical applications.

In vitro degradation of pure magnesium―the synergetic influences of glucose and albumin

The biocorrosion of magnesium in the external physiological environment is still difficult to accurately evaluate the degradation behavior in vivo, particularly, in the microenvironment of the patients with hyperglycemia or diabetes. Thus, we explored the synergistic effects of glucose and protein on the biodegradation of pure magnesium, so as to have a deeper understanding the mechanism of the degradation in vivo. The surface morphology and corrosion product composition of pure magnesium were investigated using SEM, EDS, FTIR, XRD and XPS. The effect of glucose and albumin on the degradation rate of pure magnesium was investigated via electrochemical and immersion tests. The adsorption of glucose and albumin on the sample surface was observed using fluorescence microscopy. The results showed that the presence of 2 g/L glucose changed the micromorphology of corrosion products on the magnesium surface by reacting with metal cations, thus inhibiting the corrosion of pure magnesium. Protein formed a barrier layer to protect the magnesium at early stage of immersion. The chelation reaction between protein and magnesium surface might accelerate the degradation at later stage. There may be a critical glucose (albumin) content. Biodegradation of pure magnesium was inhibited at low concentrations and promoted at high concentrations. The synergistic effect of glucose and protein restrained the adsorption of aggressive chloride ions to a certain extent, and thus inhibited the degradation of pure magnesium considerably. Moreover, XPS results indicated that glucose promoted the adsorption of protein on the sample surface.

Improving the Antibacterial Property of Chitosan Hydrogel Wound Dressing with Licorice Polysaccharide

A series of hydrogels with different ratios of chitosan and licorice polysaccharide (LP) were prepared by crosslinking to different concentrations of genipin (gp). They were characterized by FTIR (Fourier transform infrared spectroscopy), SEM (Scanning electron microscope), swelling ratio, rheological measurements, degradation with time, cytotoxicity, and antibacterial efficacy. Results show that the hydrogels have porous structures. With an increase in LP content, the swelling rate grows in the early stage of immersion in buffer and drops later. The swelling ratio ranged from 986% to 1677%, and stiffness varied from 777 Pa to 1792 Pa. The addition of LP reduced the mechanical strength and delayed gelation and degradation of the hydrogels. However, the most important discovery was that gp increases the viability of NIH 3T3 cells from 94% to 137%, and LP raises the bacteriostatic efficacy from 51% to 78%. Hydrogels synthesized from 1% genipin, 3% chitosan, and 4% licorice polysaccharide showed the best antibacterial and fibroblast proliferation promoting activities. They exhibited moderate swelling and degradation rates over time, while being more suitable to affect healing of chronic wound infections. These results provide a new strategy to improve the antibacterial effectiveness and cyto-compatibility of chitosan hydrogels with water soluble active LPs from Glycyrrhiza that derive from traditional Chinese medicine.

Electrochemical corrosion behavior of micrometer‐sized porous Ti3SiC2 compounds in NaCl solution

The electrochemical corrosion behavior of reactively synthesized porous Ti3SiC 2 in 1 M NaCl solution was investigated compared with that of porous TiAl. Porous Ti 3SiC 2 and TiAl have high‐purity single phase characteristics with open porosities of 48% and 41% and maximum apertures of 6.9 and 16.3 µm, respectively, which show positive direction shift behavior of open circuit potential at the early immersion stage with stable potential ranges of 0.25~0.27 and −0.36~−0.38 V (vs. saturated calomel), respectively. The electrochemical corrosion procedures of the two porous compounds exhibit three‐stage characteristics of activation, passivation, and breakdown. Porous Ti 3SiC 2 has a much larger ohmic resistance and Warburg coefficient than TiAl. The passivation films of the two porous compounds consist of an inner layer with a larger resistance and a porous outer layer. Porous Ti 3SiC 2 has a significantly larger resistance and relatively smaller capacitance for the inner passivation film, indicating better corrosion resistance stability.

On microstructure and early stage corrosion performance of heat treated direct metal laser sintered AlSi10Mg

This study examines the impact of low-temperature heat-treatment on the microstructure and corrosion performance of direct metal laser sintered (DMLS)-AlSi10Mg alloy. Differential scanning calorimetry (DSC) was used to determine the phase(s) transition temperatures in the alloy. Two exothermic phenomena were detected and associated with the Mg2Si precipitation and Si phase precipitation in the as-printed alloy. Based on DSC results, thermal-treatments including below and above the active Si precipitation temperature at 200 °C and 300 °C, respectively, and 350 °C as an upper limit temperature for 3 h were applied to the as-printed samples. Scanning electron microscopy and X-ray diffraction analysis confirmed that heat-treatment from 200 °C to 350 °C promotes the homogeneity of the microstructure, characterized by uniform distribution of eutectic Si in α-Al matrix. To investigate the impact of the applied heat-treatment cycles on corrosion resistance of DMLS-AlSi10Mg at early stage of immersion, anodic polarization testing and electrochemical impedance spectroscopy were performed in aerated 3.5 wt.% NaCl solution. The results revealed more uniformly distributed pitting attack on the corroded surfaces by increasing the heat-treatment temperature up to 300 °C, attributed to the more protective nature of the spontaneously air-formed passive layer on the surface of the alloy at initial immersion time. Further increase of the heat treatment temperature to 350 °C induced severe localized corrosion attacks near the coarse Si particles, ascribed to the increased potential difference between the coalesced Si particles and aluminum matrix galvanic couple. In comparison, the corrosion of the as-printed and 200 °C heat treated samples was characterized by a penetrating selective attack along the melt pool boundaries, leading to a higher corrosion current density and an active surface at early exposure, associated with the weakness of the existing passive film on their surfaces.

Wollastonite/forsterite composite scaffolds offer better surface for hydroxyapatite formation

The present work deals with a comparative study of ceramic/ceramic composites for the development of scaffolds for biomedical applications. Wollastonite and forsterite were synthesized by a sol–gel combustion method. The influence of constituents and composition on apatite deposition was studied by fabricating wollastonite/forsterite composites. The X-ray diffraction pattern explains the bone like-apatite deposition within early stages of immersion. The atomic force microscopy micrographs revealed that with an increase in wollastonite content in the composites the roughness was enhanced. Dissolution studies further confirmed the rapid consumption of Ca and P ions from the simulated body fluid. Hence, apatite formation was observed to be more on the surface of a composite containing a higher amount of wollastonite. The results suggest that composites have more influence on the biomineralization activity when compared with pure bioceramics.

Effect of specimen thicknesses on water absorption and flexural strength of CFRP laminates subjected to water or alkaline solution immersion

Abstract In this paper, an experimental research was undertaken to investigate the effect of specimen thicknesses on water absorptions and flexural strengths of wet lay-up CFRP laminates subjected to distilled water or alkaline solution immersion up to 180 days. Test results showed that the water uptake and flexural strength retention of CFRP laminates were significantly affected by the adopted specimen thickness. For the same aging time, the water uptake of CFRP laminates decreased in the early stage of immersion and increased in the later stage of immersion with the increase of specimen thickness. Meanwhile, the flexural strength retention generally increased as specimen thickness increased. In addition, a new thickness-based accelerated method for hygrothermal aging test of CFRP laminates was proposed. The accelerated factors of the water uptake and flexural strength retention of CFRP laminates were theoretically deduced. The proposed analytical model of accelerated factors was verified with current test data, and then applied to predict long-term properties of CFRP laminates. Compared with the traditional temperature-based accelerated method, the new thickness-based accelerated method is much easier to apply to predict long-term properties of CFRP laminates with good accuracy.

Galvanic Corrosion between AA5052 Aluminum-Magnesium Alloy and SS400 Carbon Steel in Aqueous NaCl Solutions

Aluminum alloys (AAs) such as 5000 and 6000 series have relatively good corrosion resistance and plastic workability. The use of these alloys for automobile body parts have been increased significantly because of reducing the weight of the body and improvement of fuel consumption. Although aluminum alloys themselves have relatively good corrosion resistance in atmospheric environments, galvanic corrosion between aluminum alloys and other materials should be taken into consideration in automotive application. This is because the body parts made of Al alloys should be jointed with other materials such as steel parts by welding or other methods and because the jointed parts can be exposed to atmospheric corrosion environments. In this study, galvanic corrosion between AA5052 aluminum-magnesium alloy and SS400 carbon steel is investigated in aqueous NaCl solutions. A galvanic couple used in this study was made of AA5052 Al-Mg alloy (Mg: 2.65, Si: 0. 08, Fe: 0.27, Cu: 0.01, Cr: 0.17, Zn: 0.01, Al: bal. (mass%)) and carbon steel (JIS SS400). The plate of both materials was 10-mm width and 15-mm length and 3-mm thickness. After lead wire was connected on the surface of the plates, they were embedded in an epoxy resin. At this time, both materials were separated with a 500-um gap. The surface of the galvanic couple was polished with SiC papers up to JIS 2000 grit and rinsed in EtOH. Galvanic corrosion experiments were performed for 2 hours in aqueous NaCl solutions at various concentrations ranging from 5 mM to 500 mM. Galvanic current and corrosion potential of the couple were measured during the immersion in the solutions. Corrosion potentials measured during the immersion in various concentrations of NaCl were almost constant for 2 h. The corrosion potentials changed in the less noble direction with increasing NaCl concentrations. Galvanic currents between AA5052 and SS400 showed positive values during the immersion in the solutions, indicating that aluminum-alloy side was basically an anode. However, the galvanic currents were almost the same independent of NaCl concentrations. From the results of polarization behaviors for AA5052 and SS400, a significant anodic current increase due to the propagation of localized corrosion on aluminum alloy and a diffusion-limiting current for the reduction of dissolved oxygen on steel were observed at the corrosion potentials of the galvanic corrosion tests. These results indicated that galvanic corrosion between AA5052 and SS400 in the early stage of immersion progressed under a diffusion-limiting condition for the reduction of dissolved oxygen on steel.

Anodic Polarization Behavior of Zn in Aqueous MgCl2 Solutions

Zn-coated steels have been widely used in various engineering fields such as automobiles and construction since they have excellent corrosion resistance in mild atmospheric corrosion environments. In order to improve the corrosion resistance of the coated steels, Zn-alloy coatings containing Mg have been newly developed and they have shown superior corrosion resistance compared with Zn coatings. However, Mg should dissolve into aqueous solutions preferentially in the early stage of immersion because of one of the active metallic elements. Therefore, it is considered that better corrosion resistance of Zn-alloy coatings containing Mg is related to the presence of Mg2+ ions in corrosion media. However, the effect of Mg2+ on the improvement of corrosion resistance of Zn and Zn-alloy coatings is still unclear. In this study, the effect of Mg2+ on the anodic polarization behavior of Zn is investigated by polarization measurements. The sample used in this study was Zn plate (99.7%, Goodfellow) and it was ground down to JIS#2000 grit with SiC abrasive papers. Then the sample was degreased in EtOH ultrasonically and then dried in air. The test solutions was aqueous solutions of MgCl2 of various concentrations ranging from 0.05 M to 1 M. Some of the test solutions were deaerated by Ar bubbling at least for 20 h. Anodic polarization curves for Zn were measured at a sweep rate of 0.5 mV/s. Anodic polarization curves for Zn was measured both in aerated and deaerated MgCl2 solutions. For the measurements in deaerated solutions, cathodic polarization treatment for reduction of air-formed films on Zn was done in NaCl solutions without Mg2+ ions. It was found from the results of anodic polarization curves for Zn in aerated MgCl2 solutions that anodic dissolution was enhanced with increasing concentration of MgCl2 and that Tafel slopes of the curves were relatively small in the order of tens of mV/dec independent of MgCl2 concentration. On the other hand, anodic polarization behaviors of Zn in deaerated MgCl2 solution were almost the same with Tafel slopes of 40 mV/dec independent of MgCl2 concentration. These results suggests that surface film formed on Zn in aerated solutions is related to anodic polarization behavior in MgCl2 solutions and that Mg2+ ions improve anti-corrosion performance of the film.

Experimental Study on the Effect of Moisture Content on Hysteretic Behavior of Reinforced Concrete Columns

Background:For reinforced concrete structures under different humid conditions, the mechanical properties of concrete are significantly affected by the moisture content, which may result in a great change of the functional performance and bearing capacity.Objective:This paper presents an experiment to investigate the influence of the moisture content on the dynamic characteristics and hysteretic behavior of reinforced concrete column.Results:The results show that the natural frequency of reinforced concrete columns increases quickly at an early stage of immersion, but there is little change when the columns are close to saturation; the difference between the natural frequencies before and after cyclic test grows as the moisture content rises. The damping ratio slightly decreases first and then increases with the increase of moisture content; the damping ratio after the cyclic test is larger than before the test due to the development of the micro-cracks.Conclusion:The trend of energy dissipation is on the rise with increasing moisture content, although at an early stage, it decreases slightly. According to the experimental result, a formula for the moisture content on the average energy dissipation of reinforced concrete columns is proposed.

Hybrid coatings with collagen and chitosan for improved bioactivity of Mg alloys

Bi-layered functional coatings were developed to modify the surface of AZ31 and ZE41 Mg alloys intended as biodegradable implant materials for bone repair applications. An inner silane-TiO2 coating was designed to slow down the degradation rate of Mg alloys while a top-most biopolymer layer, composed of collagen or chitosan, was intended to improve bioactivity and biocompatibility of the alloys. Scanning vibrating electrode technique (SVET) and scanning ion-selective technique (SIET) were used to study the localized corrosion phenomena and localized pH evolution of silane-TiO2 coated Mg alloys. Electrochemical impedance spectroscopy (EIS) evaluation of bi-layered alloys in simulated body fluid (SBF) showed that the presence of the biopolymers promotes formation of carbonated compounds within the corrosion products. Localized electrochemical impedance spectroscopy (LEIS) results revealed that the top-most biopolymer layers do not have a detrimental effect on the barrier properties of the silane-TiO2 coating. Indeed, formation of gas pockets evidenced that collagen and chitosan layers trap evolved H2 avoiding its release into the solution at early stages of immersion. The here reported results contribute to elucidate the corrosion mechanisms of AZ31 and ZE41 Mg alloys on the long term in a biological context and provide promising insights into their control via a multilayered coatings.

The adsorption and inhibition behavior of two organic inhibitors for carbon steel in simulated concrete pore solution

The inhibition behavior of calcium lignosulfonate and sodium oleate for carbon steel in simulated concrete pore solution was studied. Calcium lignosulfonate showed good inhibition for steel in simulated concrete pore solution even after 7200h immersion. The molecular simulation indicated that sinapyl alcohol in calcium lignosulfonate is the preferred site for accepting electrons. The microscopic infrared imaging showed that at the early stage of immersion the characteristic adsorption happened for calcium lignosulfonate at the corroded areas on the surface, which would be helpful for preventing initiation and propagation of both general and pitting corrosion.

Multiscale Electrochemical Study of Welded Al Alloys Joined by Friction Stir Welding

Friction stir welding (FSW) is an efficient way to join high strength aluminum alloys. However, FSW generates different microstructural areas in contact that may give rise to galvanic couplings, affecting the corrosion resistance of the assembly. In the present work, a multiscale electrochemical study of the 7475-T651 and 2024-T3 aluminum alloys butt-joined by FSW was carried out. Much lower impedances were associated with the FSW affected zones compared to the two aluminum base metals tested individually. Corrosion of the welded system resulted in the establishment of galvanic coupling, shown by local electrochemical impedance spectroscopy (LEIS) measurements, at which the AA7475 behaves anodically with respect to the AA2024. A Zn deposit was observed on the intermetallic particles of the AA2024 after 24 h of immersion in the electrolyte resulting from the galvanic coupling, which seems to reduce the galvanic coupling effects. Such a behavior in combination with LEIS results allowed a description of the galvanic coupling development between two different aluminum alloys (AA2024-T3 and AA7475-T761) butt-welded by FSW as a function of time from the early stage of immersion.

Corrosion behavior of low pressure cold sprayed Zn-Ni composites coating

PurposeThis paper aims to examine the performance of low-pressure cold-sprayed zinc–nickel (Zn-Ni) composites coating, i.e. whether it has the same performance as Zn-Ni alloy coating.Design/methodology/approachIn this paper, Zn-Ni composites coatings containing four different nickel contents were prepared with commercial DYMET 413 low-pressure cold spraying system under the same parameters. Corrosion behaviors of four kinds of coatings were examined with potentiodynamic polarization curves and electrochemical impedance spectroscopy methods, combined with scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction.FindingsCorrosion behavior of Zn-Ni composites coating is similar to Zn-Ni alloy coating. In the early stages of immersion, the anodic dissolution of zinc happens, which results in the formation of a zinc hydroxide layer. With the continuous infiltration of chloride ion, zinc hydroxide will get converted to zinc oxide, basic zinc chloride and basic zinc carbonate. The presence of nickel in coatings can prevent zinc hydroxide from converting into zinc oxide.Research limitations/implicationsFurther research should be done on improving the deposition efficiency, as the deposition efficiency of low-pressure cold spray is lower than 30 per cent.Practical implicationsLow-pressure cold spray coating can be used in cyclic dry/wet conditions to prolong the life of a steel structure.Social implicationsLow-pressure cold spray Zn-Ni coating is an environmentally friendly anticorrosion method which can be used as an alternative of hexavalent chromium passivation coating.Originality/valueZn-Ni composite coating can be deposited on steel directly by low-pressure cold spray by mechanically mixing the powders together. The composite coating also has the same long-term anticorrosion performance as Zn-Ni alloy coating.

Effect of Cr on the passive film formation mechanism of steel rebar in saturated calcium hydroxide solution

Passive films grow on the surface of Cr-modified steels subjected to saturated Ca(OH)2 solution. Electrochemical techniques, such as measurement of open circuit potentials, polarization curves, and electrochemical impedance spectroscopy combined with X-ray photoelectron spectrometer and auger electron spectroscopy, were applied to study the influence of low Cr content on the passive film formation mechanism of steel rebar in saturated Ca(OH)2 solution. Results show that Cr inhibits the formation of passive film at the beginning of its formation. Corrosion current density decreases and polarization resistance increases with the extension of the immersion time. A stable passive film takes at least three days to form. The passive film resistance of HRB400 carbon steel is higher than that of Cr-modified steels in the early stage of immersion (<72h). The polarization resistance of Cr-modified steel is larger after a stable passive film is formed (>72h), and Cr promotes the formation of a denser and more compact passive film. The stable passive film is primarily made up of iron oxides with a thickness of 5–6nm. Cr are involved in the formation of passive films, thereby resulting in a film that consists of an inner layer that contains Cr–Fe oxides and an outer layer that contains Fe oxides, whose thickness presents a slight increase as the content of Cr increases.

Corrosion resistance of porous NiTi biomedical alloy in simulated body fluids

The corrosion performance of two porous NiTi in physiological and Hank’s solutions was investigated by potentiodynamic polarization, cyclic polarization and impedance spectroscopy. Electric models simulating the corrosion mechanism at early stages of immersion were proposed, accounting for both microstructural observations and electrochemical results. Results indicate that both porous samples were susceptible to localized corrosion. The porosity increase (from 7% to 18%) resulted in larger and wider pore openings, thus favoring the corrosion resistance of 18% porous NiTi. Strengthening of corrosion resistance was observed in Hank’s solution. The pore morphology and micro-galvanic corrosion phenomena were determining factors affecting the corrosion resistance.

In-vitro bioactivity, biocompatibility and dissolution studies of diopside prepared from biowaste by using sol–gel combustion method

Diopside was synthesized from biowaste (Eggshell) by sol–gel combustion method at low calcination temperature and the influence of two different fuels (urea, l-alanine) on the phase formation temperature, physical and biological properties of the resultant diopside was studied. The synthesized materials were characterized by heating microscopy, FTIR, XRD, BET, SEM and EDAX techniques. BET analysis reveals particles were of submicron size with porosity in the nanometer range. Bone-like apatite deposition ability of diopside scaffolds was examined under static and circulation mode of SBF (Simulated Body Fluid). It was noticed that diopside has the capability to deposit HAP (hydroxyapatite) within the early stages of immersion. ICP-OES analysis indicates release of Ca, Mg, Si ions and removal of P ions from the SBF, but in different quantities from diopside scaffolds. Cytocompatability studies on human bone marrow stromal cells (hBMSCs) revealed good cellular attachment on the surface of diopside scaffolds and formation of extracellular matrix (ECM). This study suggests that the usage of eggshell biowaste as calcium source provides an effective substitute for synthetic starting materials to fabricate bioproducts for biomedical applications.