Early Stages Of Testing Research Articles

Study on the mechanical properties of X80 pipeline steel under pre-charged high-pressure gaseous hydrogen

Hydrogen embrittlement (HE) is a significant challenge to the safe operation of hydrogen-blended natural gas pipelines. The mechanical properties of X80 steel were studied after H2 pre-charging followed by mechanical properties tests and fracture morphologies observation. Results indicated that the hydrogen saturation time of X80 steel was roughly 48 h. H2 pre-charging induced a decline in strength, plasticity, and fatigue properties, while hydrogen-assisted fatigue crack growth occurred during the early stage of fatigue crack growth rate tests. Additionally, the fracture morphologies transited from primary microvoid coalescence to a mixed mode characterized by ductile dimples and quasi-cleavage planes with increasing hydrogen. The HE susceptibility increased with increasing hydrogen partial pressure and decreasing loading frequency, but there existed a critical value (6 × 10−7 s−1) for the strain rate effect on the HE susceptibility. Under H2 pre-charging conditions, the predominant HE mechanism was the hydrogen-enhanced local plasticity (HELP) mediated hydrogen-enhanced decohesion (HEDE) mechanism.

Increased long-term stress-rupture life of a nickel-based superalloy with relatively homogeneous duplex grain size distributions

Effect of duplex grain size distributions on long-term stress-rupture life of Nimonic 80A had been investigated. Non-homogeneous duplex grain size distributions greatly reduce stress-rupture life. Regional distribution of coarse grains is not good for acquiring high stress-rupture life, the formation of micro-void and propagation of cracking can easily occur at coarse grain boundary especially when plate-like Cr23C6 precipitates at grain boundary. But relatively homogeneous duplex grain size distributions are of great significance in improving stress-rupture life, the samples even possess the life value up to more than 13000 h especially when hardly any plate-like Cr23C6 precipitates at grain boundaries. Slightly increased Ti/Al ratio from 1.65 to 1.80 is also beneficial to obtain a significantly longer stress-rupture life value. The γ′ phase is very stable and always exhibits a coherent orientation relationship with γ on {100} and {111} atomic planes. The grain boundary cracking will not easily appear at the early stage of stress-rupture test in the samples with relatively homogeneous grain size distribution, the coarse and adjacent fine grains possess better deformation adjustability, and the grain boundary cracking is apparently wider and the number of cracking is also getting more after fracture for the sample with longer stress-rupture life.

Sustainable Knowledge, Awareness, Attitude And Intention For Gen-Z Sustainable Consumption

Knowledge, awareness, and attitude towards sustainable consumption have an impact on someone to change behavior that is more towards sustainable consumption. This research aims to provide the effect of sustainable knowledge, sustainable awareness, and attitude towards sustainable consumption on intention to sustainable consumption. Data collection techniques using self-administered surveys are self-administered by distributing online questionnaires. The sampling method uses non-probability sampling techniques with purposive sampling. The criteria used in the sampling of this study were Gen-Z who knew sustainable products. The statistical test used in this study to test the data was regression analysis using SPSS 26. In the early stages of testing and data analysis, instrument testing was carried out with validity tests and reliability tests as well as classical assumption tests. Testing of models and hypotheses is done with T-test and T-test. According to this research, sustainable knowledge has no effect on Intention toward sustainable consumption, sustainable awareness affects Intention toward sustainable consumption, and attitude toward sustainable consumption affects intention toward sustainable consumption.

Perspectives of African stakeholders on gene drives for malaria control and elimination: a multi-country survey

BackgroundGene drive modified mosquitoes (GDMMs) have the potential to address Africa’s persistent malaria problem, but are still in early stages of development and testing. Continuous engagement of African stakeholders is crucial for successful evaluation and implementation of these technologies. The aim of this multi-country study was, therefore, to explore the insights and recommendations of key stakeholders across Africa on the potential of GDMMs for malaria control and elimination in the continent.MethodsA concurrent mixed-methods study design was used, involving a structured survey administered to 180 stakeholders in 25 countries in sub-Saharan Africa, followed by 18 in-depth discussions with selected groups and individuals. Stakeholders were drawn from academia, research and regulatory institutions, government ministries of health and environment, media and advocacy groups. Thematic content analysis was used to identify key topics from the in-depth discussions, and descriptive analysis was done to summarize information from the survey data.ResultsDespite high levels of awareness of GDMMs among the stakeholders (76.7%), there was a relatively low-level of understanding of their key attributes and potential for malaria control (28.3%). When more information about GDMMs was provided to the stakeholders, they readily discussed their insights and concerns, and offered several recommendations to ensure successful research and implementation of the technology. These included: (i) increasing relevant technical expertise within Africa, (ii) generating local evidence on safety, applicability, and effectiveness of GDMMs, and (iii) developing country-specific regulations for safe and effective governance of GDMMs. A majority of the respondents (92.9%) stated that they would support field trials or implementation of GDMMs in their respective countries. This study also identified significant misconceptions regarding the phase of GDMM testing in Africa, as several participants incorrectly asserted that GDMMs were already present in Africa, either within laboratories or released into the field.ConclusionIncorporating views and recommendations of African stakeholders in the ongoing research and development of GDMMs is crucial for instilling stakeholder confidence on their potential application. These findings will enable improved planning for GDMMs in Africa as well as improved target product profiles for the technologies to maximize their potential for solving Africa’s enduring malaria challenge.

Early stage of erythrocyte sedimentation rate test: Fracture of a high-volume-fraction gel.

Erythrocyte sedimentation rate (ESR) is a clinical parameter used as a nonspecific marker for inflammation, and recent studies have shown that it is linked to the collapse of the gel formed by red blood cells (RBCs) at physiological hematocrits (i.e. RBC volume fraction). Previous research has suggested that the observation of a slower initial dynamics is related to the formation of fractures in the gel. Moreover, RBC gels present specific properties due to the anisotropic shape and flexibility of the RBCs. Namely, the onset of the collapse is reached earlier and the settling velocity of the gel increases with increasing attraction between the RBCs, while the gel of spherical particles shows the opposite trend. Here, we report experimental observations of the gel structure during the onset of the collapse. We suggest an equation modeling this initial process as fracturing of the gel. We demonstrate that this equation provides a model for the motion of the interface between blood plasma and the RBC gel, along the whole time span. We also observe that the increase in the attraction between the RBCs modifies the density of fractures in the gel, which explains why the gel displays an earlier onset when the aggregation energy between the RBCs increases. Our work uncovers the detailed physical mechanism underlying the ESR and provides insights into the fracture dynamics of an RBC gel. These results can improve the accuracy of clinical measurements.

Valved Conduits for Right Ventricular Outflow Tract Reconstruction: A Review of Current Technologies and Future Directions.

The need for right ventricular outflow tract reconstruction is common and growing in congenital heart surgery given expanding indications for the repair of congenital as well as acquired heart disease. Various valved conduit options currently exist including homografts, xenograft pulmonary valved conduits (Contegra™), and porcine valved conduits. The major limitation for all conduits is implant durability, which requires reoperation. Currently, cryopreserved homografts are often used given their superiority shown in long-term data. Significant limitations remain in the cost and availability of the graft, particularly for smaller sizes. Contegra conduits are available in a variety of sizes. Nonetheless, the data regarding long-term durability are less robust and studies comparing durability with homografts have been conflicting. Additionally, there is concern for increased rates of late endocarditis in this conduit. Porcine valved conduits offer a reliable option but are limited by structural valve degeneration associated with all types of bioprosthetic heart valve replacements. New developments in the field of tissue engineering have produced promising bio-restorative valved conduits that may overcome many of the limitations of previous conduit technologies. These remain in the early stages of clinical testing. This review summarizes the clinical data surrounding the conduits used most commonly in clinical practice today and explores emerging technologies that may bring us closer to developing the ideal conduit.

A Flexible PDMS-Based Optical Biosensor for Stretch Monitoring in Cardiac Tissue Samples

Cardiotoxicity, characterized by adverse impacts on normal heart function due to drug exposure, is a significant concern due to the potentially serious side effects associated with various pharmaceuticals. It is essential to detect the cardiotoxicity of a drug as early as possible in the testing phase of a medical composite. Therefore, there is a pressing need for more reliable in vitro models that accurately mimic the in vivo conditions of cardiac biopsies. In a functional beating heart, cardiac muscle cells are under the effect of static and cyclic stretches. It has been demonstrated that cultured cardiac biopsies can benefit from external mechanical loads that resemble the in vivo condition, increasing the probability of cardiotoxicity detection in the early testing stages. In this work, a biosensor is designed and fabricated to allow for stretch monitoring in biopsies and tissue cultures using an innovative sensing mechanism. The detection setup is based on a biocompatible, thin, flexible membrane—where the samples are attached—which is used as an optical waveguide to detect pressure-caused shape changes and stretches. Various prototypes have been fabricated with a cost-effective process, and different measurements have been carried out to experimentally validate the proposed measurement technique. From these evaluations, stretches of up to 1.5% have been measured, but the performed simulations point towards the possibility of expanding the considered technique up to 10–30% stretches.

Methods for evaluating effects of transgenes for quantitative traits

AbstractTransgenes that improve quantitative traits have traditionally been evaluated in one or a few genetic backgrounds across multiple environments. However, testing across multiple genetic backgrounds can be equally important to accurately quantify the value of a transgene for breeding objectives. Creating near‐isogenic lines across a wide germplasm space is costly and time consuming, which renders it impractical during early stages of testing. In this experiment, we evaluate three approaches to sample the genetic space while concurrently testing across environments. We created both transgenic and non‐transgenic doubled haploid maize (Zea mays L.) lines, F2:3 lines, and bulk F3 families to determine if all methods resulted in similar estimation of transgene value and to identify the number of yield trial plots from each method necessary to obtain a stable estimate of the transgene value. With one exception, the three methods consistently estimated a similar effect of the transgene. We suggest that bulked F3 lines topcrossed to a tester inbred is the most effective method to estimate the value of a transgene across both genetic space and environments.

Twenty Years of Progress in Microstructure Modelling for Ultrasonic Testing, from Shielded Metal Arc Welding to Gas Tungsten Arc Welding: An Analysis for Future Developments

To ensure and to demonstrate the mechanical integrity of a welded structure, precise ultrasonic testing (UT) is often mandatory. The importance of the link between nondestructive testing (NDT) and the assessment of structural integrity is recalled. However, it is difficult to achieve great efficiency as the welding of thick and heavy structural part produces heterogeneous material. Heterogeneity results from the welding process itself as well as from the material solidification laws. For thick components, several welding passes are deposited, and temperature gradients create material grain elongation and/or size variations. In many cases, the welded material is also anisotropic, this anisotropy being due to the metal used, for example, austenitic stainless steel. At the early stages of ultrasonic testing, this kind of welded material was considered too unpredictable, and thus too difficult to be tested by ultrasounds without possible diagnosis errors and misunderstandings. At the end of the 1990s, an algorithmic solution to predict the material organisation began to be developed using data included in the welding notebook. This algorithm or modelling solution was called MINA. This present work recalls, in a synthetic form, the path followed to create this algorithm combining the use of solidification laws and the knowledge of the order of passes in the case of shielded metal arc welding (SMAW). This work describes and questions the simplifications used to produce a robust algorithm able to give a digital description of the material for wave simulation code. Step by step, advances and demonstrations are described as well as the limitations, and ways to progress are sketched. Recent developments are then explained and discussed for modelling in the case of gas tungsten arc welding (GTAW), in addition to discussions about 3D modelling for the future. The discussion includes alternative ways to represent the welded material and challenges to continue to produce more and more convincing weld material model to qualify and to make use of UT methods.

Bispecific T-Cell Engagers and Chimeric Antigen Receptor T-Cell Therapies in Glioblastoma: An Update.

Glioblastoma is the most common malignant primary brain tumor in adults. The prognosis is extremely poor even with standard treatment of maximal safe resection, radiotherapy, and chemotherapy. Recurrence is inevitable within months, and treatment options are very limited. Chimeric antigen receptor T-cell therapy (CART) and bispecific T-cell engagers (TCEs) are two emerging immunotherapies that can redirect T-cells for tumor-specific killing and have shown remarkable success in hematological malignancies and been under extensive study for application in glioblastoma. While there have been multiple clinical trials showing preliminary evidence of safety and efficacy for CART, bispecific TCEs are still in the early stages of clinical testing, with preclinical studies showing very promising results. However, there are multiple shared challenges that need to be addressed in the future, including the route of delivery, antigen escape, the immunosuppressive tumor microenvironment, and toxicity resulting from the limited choice of tumor-specific antigens. Efforts are underway to optimize the design of both these treatments and find the ideal combination therapy to overcome these challenges. In this review, we describe the work that has been performed as well as novel approaches in glioblastoma and in other solid tumors that may be applicable in the future.

Environmental DNA reveals invasive crayfish microbial associates and ecosystem‐wide biodiversity before and after eradication

AbstractBiodiversity monitoring in conservation projects is essential to understand environmental health, complexity, and recovery. However, traditional field surveys can be expensive, time‐consuming, biased toward visual detection, and/or only measure a limited set of taxa. Environmental DNA (eDNA) methods provide a new approach to biodiversity monitoring that has the potential to sample a taxonomically broader set of organisms with a similar effort, but many of these approaches are still in the early stages of development and testing. Here, we used multi‐locus eDNA metabarcoding to understand how the removal of invasive red swamp crayfish using cypermethrin pesticide impacts local biodiversity of a desert oasis ecosystem, as well as to detect crayfish both directly and indirectly. We tracked crayfish DNA signatures, microbial DNA associated with crayfish, and biodiversity of plant, fungal, animal, and bacterial communities through time. We were unsuccessful in detecting crayfish directly in either control tanks or oases using targeted metabarcoding primers for invertebrates and eukaryotes, similar to previous studies which have shown variable levels of success in detecting crayfish from environmental samples. However, we were successful in discerning a suite of 90 crayfish‐associated taxa to serve as candidate bioindicators of invasive presence using 16S and fungal ITS2 metabarcoding. Ranking these 90 taxa by their geographic distribution in eDNA surveys and by evidence of crayfish associations in the literature, we support nine taxa to be high ranking, and suggest they be prioritized in future biomonitoring. Biodiversity analyses from five metabarcode loci including plants, animals, and both prokaryotic and eukaryotic microbes showed that communities differed but that species richness remained relatively similar between oases through time. Our results reveal that, while there are limitations of eDNA approaches to detect crayfish and other invasive species, microbial bioindicators offer a largely untapped biomonitoring opportunity for invasive species management, adding a valuable resource to a conservation manager's toolkit.

Self-ion irradiation effects on nanoindentation-induced plasticity of crystalline iron: A joint experimental and computational study

In this paper, experimental work is supported by multi-scale numerical modeling to investigate nanomechanical response of pristine and ion irradiated with Fe2＋ ions with energy 5 MeV high purity iron specimens by nanoindentation and Electron Backscatter Diffraction. The appearance of a sudden displacement burst that is observed during the loading process in the load–displacement curves is connected with increased shear stress in a small subsurface volume due to dislocation slip activation and mobilization of pre-existing dislocations by irradiation. The molecular dynamics (MD) and 3D-discrete dislocation dynamics (3D-DDD) simulations are applied to model geometrically necessary dislocations (GNDs) nucleation mechanisms at early stages of nanoindentation test; providing an insight to the mechanical response of the material and its plastic instability and are in a qualitative agreement with GNDs density mapping images. Finally, we noted that dislocations and defects nucleated are responsible the material hardness increase, as observed in recorded load–displacement curves and pop-ins analysis.

Evolution of a System to Monitor Infant Neuromotor Development in the Home: Lessons from COVID-19

In the nine months leading up to COVID-19, our biomedical engineering research group was in the very early stages of development and in-home testing of HUGS, the Hand Use and Grasp Sensor (HUGS) system. HUGS was conceived as a tool to allay parents' anxiety by empowering them to monitor their infants' neuromotor development at home. System focus was on the evolving patterns of hand grasp and general upper extremity movement, over time, in the naturalistic environment of the home, through analysis of data captured from force-sensor-embedded toys and 3D video as the baby played. By the end of March, 2020, as the COVID-19 pandemic accelerated and global lockdown ensued, home visits were no longer possible and HUGS system testing ground to an abrupt halt. In the spring of 2021, still under lockdown, we were able to resume recruitment and in-home testing with HUGS-2, a system whose key requirement was that it be contactless. Participating families managed the set up and use of HUGS-2, supported by a detailed library of video materials and virtual interaction with the HUGS team for training and troubleshooting over Zoom. Like the positive/negative poles of experience reported by new parents under the isolation mandated to combat the pandemic, HUGS research was both impeded and accelerated by having to rely solely on distance interactions to support parents, troubleshoot equipment, and securely transmit data. The objective of this current report is to chronicle the evolution of HUGS. We describe a system whose design and development straddle the pre- and post-pandemic worlds of family-centered health technology design. We identify and classify the clinical approaches to infant screening that predominated in the pre-COVID-19 milieu and describe how these procedural frameworks relate to the family-centered conceptualization of HUGS. We describe how working exclusively through the proxy of parents revealed the family's priorities and goals for child interaction and surfaced HUGS design shortcomings that were not evident in researcher-managed, in-home testing prior to the pandemic.

Wireless monitoring devices in hospitalized children: a scoping review.

The purpose of this study is to provide a structured overview of existing wireless monitoring technologies for hospitalized children. A systematic search of the literature published after 2010 was conducted in Medline, Embase, Scielo, Cochrane, and Web of Science. Two investigators independently reviewed articles to determine eligibility for inclusion. Information on study type, hospital setting, number of participants, use of a reference sensor, type and number of vital signs monitored, duration of monitoring, type of wireless information transfer, and outcomes of the wireless devices was extracted. A descriptive analysis was applied. Of the 1130 studies identified from our search, 42 met eligibility for subsequent analysis. Most included studies were observational studies with sample sizes of 50 or less published between 2019 and 2022. Common problems pertaining to study methodology and outcomes observed were short duration of monitoring, single focus on validity, and lack information on wireless transfer and data management. Conclusion: Research on the use of wireless monitoring for children in hospitals has been increasing in recent years but often limited by methodological problems. More rigorous studies are necessary to establish the safety and accuracy of novel wireless monitoring devices in hospitalized children. What is Known: •Continuous monitoring of vital signs using wired sensors is the standard of care for hospitalized pediatric patients. However, the use of wires may pose significant challenges to optimal care. What is New: • Interest in wireless monitoring for hospitalized pediatric patients has been rapidly growing in recent years. • However,most devices are in early stages of clinical testing and are limited by inconsistent clinical and technological reporting.

The immunoregulatory protein CD200 as a potentially lucrative yet elusive target for cancer therapy.

CD200 is an immunoregulatory cell surface ligand with proven pro-tumorigenic credentials via its ability to suppress CD200 receptor (CD200R)-expressing anti-tumor immune function. This definitive role for the CD200-CD200R axis in regulating an immunosuppressive tumor microenvironment has garnered increasing interest in CD200 as a candidate target for immune checkpoint inhibition therapy. However, while the CD200 blocking antibody samalizumab is still in the early stages of clinical testing, alternative mechanisms for the pro-tumorigenic role of CD200 have recently emerged that extend beyond direct suppression of anti-tumor T cell responses and, as such, may not be susceptible to CD200 antibody blockade. Herein, we will summarize the current understanding of CD200 expression and function in the tumor microenvironment as well as alternative strategies for potential neutralization of multiple CD200 mechanisms in human cancers.

Effects of ATDE Teaching Mode during Online Teaching on Creative Thinking Ability of Learners

Abstract: Online teaching based on computer and network technology has become a vital teaching mode under the current situation of the overwhelming COVID-19 epidemic. Teachers and students who fully understand and master basic knowledge and skills of various information technologies in online learning can stimulate students’ interest in learning information technology and train their ability in information collection, processing, and use. Given the lack of face-to-face communication and deep emotional exchange, online teaching has poor performance in improving the creative thinking ability of learners. During online teaching, teachers train the innovation consciousness quality of students in online courses successfully and improve the creative thinking ability of learners by using “asking,” “thinking,” “doing,” and “evaluation,” that is, the ATDE teaching mode. In this study, a teaching experiment was carried out on 41 undergraduates admitted in 2018 who majored in civil engineering at Zhengzhou University of Industrial Technology, Henan Province, China. Students were divided into the experimental group and the control group. The experimental group adopted the ATDE teaching mode for the course Design of concrete structure, while the control group applied the traditional teaching mode. A questionnaire survey of students’ creative thinking ability was conducted for both groups. Results showed that university students’ creative, emotional attitude under the ATDE teaching mode was far higher than that under the traditional (F=6.490, p=0.015). The creative thinking performance of university students under the ATDE teaching mode was higher than that under the traditional teaching mode (F=7.468, p=0.009). The Pearson correlation coefficient between creative, emotional attitude and creative thinking performance was 0.985 in the late test stage, which was higher than in the early test stage (0.480), which reveals that the ATDE teaching mode was conducive to improving abstract course learning effect of students majoring in civil engineering. Research conclusions can provide some references to investigate the feasibility of ATDE teaching mode in universities and offer a new choice and feasible path to train students’ creative thinking ability.

Preload loss of CFRP bolted joint without rotation under transverse and axial loading

There are a large number of CFRP-steel bolted joints with jet nuts subjected to transverse and axial load in aircraft structures, which have passed standard testing of locking characteristics of fasteners under transverse loading conditions. This fastener has a great preload loss and no rotation loosening occurs. Based on the observation, we designed a biaxial loading experiment for CFRP bolted joint, and measured the preload and loosening angle under different initial preload. The biaxial research suggests that compared with the uniaxial loading condition, the preload loss is larger, the rotation is minimal and only appears in the early stage of test, and there are obvious indentations on the surface of CFRP panel. Embedment as an important factor leads to the preload loss due to the weak ability of CFRP panel suffering out of plane load. In order to study the effect of embedment, the relationship between stress S22 and indentation depth under different preload, the relationship between biaxial load amplitude and embedding depth, and the ratio of the preload loss caused by embedding to the total preload loss are revealed by finite element analysis.

A cycle-durable hollow nanoneedle structure with a nanosheet as a conductive substrate CoS1.097/Ni9S8@RGO to enhance supercapacitor performance.

Metal sulfides are promising supercapacitor electrode materials with a large theoretical capacity and rich operability. However, its unsatisfactory cycle stability and rate performance are tough problems to be solved. Therefore, the preparation of metal sulfide-based electrode materials with a stable structure, long cycle life, and high-rate performance is an effective strategy to solve these problems. Herein, metal sulfides were first crystallized into crosslinked nanosheet and nanotube structures, which ensure abundant active sites for redox reactions. Then, the further modification of the prepared material by spraying graphene was carried out, which, as demonstrated by combining experimental data and physical characterization, leads to a more complete hollow structure, enlarged electrochemical reaction sites, and reduced electrolyte transport distance, thus improving the charge transfer kinetics. In the early stage of the charge-discharge cycle test, the electrode material undergoes a self-activation process, which transforms the electrode material from one equilibrium state to a new equilibrium state. Therefore, 2-CSNS@RGO electrode capacitance was 1650.13 C g-1 at 1 A g-1 with remarkable cycling of 3000 cycles at 10 A g-1, and it retains 186.1% capacity of the initial value. An asymmetric supercapacitor (2-CSNS@RGO//AC) was prepared by coupling 2-CSNS@RGO as the positive electrode and activated carbon (AC) as the negative electrode. 2-CSNS@RGO//AC has an energy density of 88 W h kg-1 at a power density of 0.8 kW kg-1, and the capacity retention rate is 131.6% after 30 000 cycles at 10 A g-1.

Plant-based model for the visual evaluation of electroporated area after irreversible electroporation and its comparison to in-vivo animal data.

Electroporation (EP) is widely used in medicine, such as cancer treatment, in form of electrochemotherapy or irreversible electroporation (IRE). For EP device testing, living cells or tissue inside a living organism (including animals) are needed. Plant-based models seem to be a promising alternative to substitute animal models in research. The aim of this study is to find a suitable plant-based model for visual evaluation of IRE, and to compare the geometry of electroporated areas with in-vivo animal data.For this purpose, a variety of fruit and vegetables were selected and visually evaluated after 0/1/2/4/6/8/12/16/24 h post-EP. Apple and potato were found to be suitable models as they enabled a visual evaluation of the electroporated area. For these models, the size of the electroporated area was determined after 0/1/2/4/6/8/12/16/24 h. For apples, a well-defined electroporated area was visual within two hours, while in potatoes it reached a plateau after eight hours only. The electroporated area of apple, which showed the fastest visual results was then compared to a retrospectively evaluated swine liver IRE dataset which had been obtained for similar conditions. The electroporated area of the apple and swine liver both showed a spherical geometry of comparable size. For all experiments, the standard protocol for human liver IRE was followed. To conclude, potato and apple were found to be suitable plant-based models for the visual evaluation of electroporated area after irreversible EP, with apple being the best choice for fast visual results. Given the comparable range, the size of the electroporated area of the apple may be promising as a quantitative predictor in animal tissue. Even if plant-based models cannot completely replace animal experiments, they can be used in the early stages of EP device development and testing, decreasing animal experiments to the necessary minimum.

Towards real time automated early gear failure detection

The ability to stop a gear fatigue test before catastrophic failure has many advantages. However, today, a widely accepted approach is not available. This case study applies a vibration-based condition monitoring methodology to detect early gear failures. The gear studied takes part in an all-wheel-drive drivetrain system. Vibration signals from four run-to-failure fatigue tests at two constant torque-speed combinations were used as input to time-synchronous averaging and autoregression model generation. The applied methodology shows promising results for early failure detection, and the process is feasible for implementation in an automated environment. Real time analysis is also possible since the autoregression model generates a healthy state TSA signal during the early testing stages. However, the time to failure detection varies with operating conditions, with low sensitivity at high-speed and low-torque conditions.