Early Seedling Stage Research Articles

Effects of ultrasonic waves on seedling growth, biochemical constituents, genetic stability of fenugreek (Trigonella foenum-graecum) under salinity stress

Fenugreek is a globally important legume that is widely cultivated for its therapeutic benefits in most parts of the world. Seeds on the other hand have a poor germination and growth rate when exposed to salinity. The effect of ultrasonic exposure period on germination and early seedling behaviors of fenugreek seeds under salt stress was investigated in a laboratory experiment. During germination and early seedling stages, all tests were conducted at 40 kHz in a water bath ultrasonic device with two durations (10 and 20 min) under salinity stress using different concentrations of NaCl (0, 1000, 3000, and 5000 mg/l). The results revealed a substantial decrease in germination percentage, all growth criteria, with increasing NaCl concentration and a significant increase in biomass produced by the Fenugreek (total soluble protein, total soluble carbohydrate, and proline), all of which are thought to be mechanisms for salinity resistance. Ultrasonication of fenugreek seeds for 10 and 20 min has a significant impact on seed germination, early seedling development and biochemical constituents under normal and stress conditions. The genetic stability of fenugreek DNA content was affected by these different treatments. This variation was estimated by RAPD-PCR molecular marker, and resulted in a total polymorphism percentage of 49.72% from all the primers. All these different treatments caused variation in the physiological responses and DNA content. This variation enhanced with more ultrasonic and salt treatments. Hence, these stresses can be used for enhancing the variable metabolic processes in fenugreek plant and stimulate its medicinal properties.

Assessment of seedling traits of rice landraces under different saline conditions

Salinity is one of the major abiotic stresses affecting rice growth and yield worldwide. In rice, the most critical stages which affect salinity at a greater level are germination, vegetative and reproductive stages.It is very important to know the genotypic variation among landraces under saline conditions at the seed germination stage to reduce the harmful effect of salinity. The present study conducted on Petri plate was mainly for assessing germination, relative water content and seedling parameters of eleven rice landraces with check landrace Pokali under three different salt concentrations (75mM, 125mM and 150mM). Two-way ANOVA gave the variations among the genotypes, treatments and their interactions. The present study showed that Mundan, Odiyan, Muttadan, Kallimadiyan and Vellimuthu had less percentage reduction in growth parameters at the germination stage. Odiyan and Mundan showed less percentage reduction in fresh weight (36.09%) and shoot length (25.61%) respectively, in relative water content (10.70% and 16.07%, respectively) at higher concentrations of salinity (150mM) compared to control. Pokali, Chembakam and Odiyan showed good germination parameters under three different saline treatments compared to other genotypes. Biplot analysis showed 65.4% variation between the treatments, whereas the variation between the genotypes was around 13.3%. Screening of landraces for salinity tolerance at the seed germination stage is the most reliable method to identify the salt tolerant line at the early seedling stage. The present study can be used for further screening programme at the vegetative stage for the identification of potential salt tolerant lines to improve breeding and gene introgression studies.

Sole and combined effect of micronutrients on germination and seedling growth attributes of sweet corn (Zea mays L. saccharata)

Micronutrient deficiency is a major constraint to crop productivity in most regions. Agronomic interventions such as micronutrient seed soaking could significantly improve seedling establishment in micronutrient deficient soils also. Iron (Fe), Manganese (Mn), and Zinc (Zn) are essential to plant metabolism and are needed in relatively small but critical amounts by plants. Little attention has been paid to studying the impact of different micronutrients application on the early seedling stage. Laboratory studies were carried out to determine the effect of different micronutrient treatments on germination, and early growth as well as biochemical analysis of seedlings. After sterilization, seeds were soaked in different micronutrient solutions for 24 hrs. The cigar roll method was used for seed germination under well-defined conditions. After seven days of germination, various seedling traits and biochemical parameters were recorded. Effects of seed soaking with different combinations of Iron, Zinc, and Manganese on growth and biochemical attributes of maize (sweet corn) were studied. The single or combined effects of the experimental treatments significantly affected the growth parameters of the seedling. There was better seedling establishment along with higher endogenous sugar, starch content as well as alpha-amylase activity at the early stages of plant development. A positive effect of seed soaking was observed in all traits through all treatments. Overall results predicted that the combination of Ferrous sulfate (0.2%) along with Zinc sulfate (0.5%) shows the best results followed by Ferrous sulfate (0.2%) along with Zinc sulfate (0.2%).

Germination and Early Seedling Growth Attributes of Rice (Oryza sativa L.) Cultivars under Different Salinity (NaCl) Stresses

The impact of different salinity stresses [0, 6.4, 12.8, 19.2, and 25.6 dS/m (NaCl)] on the final germination percentage, speed of germination, germination capacity, shoot and root length, seedling vigor index, and total plant dry weight of ten rice cultivars at germination and early seedling stages was investigated in this study. Salinity stress significantly decreased the final germination percentage, speed of germination, germination capacity, shoot and root length, and seedling dry weight in all cultivars, with the most severe effects from level 19.2 and 25.6 dS/m NaCl. Rice cultivars were classified as tolerant, moderately tolerant, moderately susceptible, and susceptible based on the reduction of dry matter, which is considered as the most limiting factor at high NaCl concentrations. The cultivars NJ9108, 3Y9H, 6Y9H, XD22, 5W01, and HLYH3 showed greater efficiency in salt tolerance during germination at 19.4 dS/m salinity. While 20H025, 6Y9H, and XD22 at the seedling stage performed better based on their dry weight reduction. The study found that NJ9108 and XD22 may be used to investigate the effects of salinity on the development processes and physiological consequences at the advanced stage of growth, as salt tolerance during germination and the early seedling stage may be varied from the developed stages. The results suggest that tolerant rice cultivars can be used to study the effects of salinity on the development and other physiological impacts at vegetative and reproductive stages. These findings will support salt-tolerant rice cultivars that may be developed for better production in terms of coping with salinity stresses in the germination and early growth stages.

Phytotoxicity level and accumulation ability of pot marigold (Calendula officinalis L.) to zinc

The study aims to reveal the effects of different Zn levels on pot marigold. We determined some germination and young seedling properties in the first experiment, and morphological, stomatal, and physiological parameters besides uptake profiles of both Zn and other plant nutrients in the second one. We supplied the water requirement of the seeds with Zn solutions (0, 250, 500, 750, and 1,000 mg l−1) in the first experiment, and We added the same zinc doses as mg kg−1 to the soil of the pots where the plants would grow in the second one. As a result, the inhibitory effects were more prominent in the early seedling stage (especially at 1,000 mg l−1 Zn) than in the germination one. We determined plant growth retardation, decreases in leaf water contents, and increases in membrane damages with higher Zn (≥500 mg kg−1) in the experiment conducted by potting soil. We detected decreases in chlorophyll parameters parallel with the increases in Zn. The alterations in plant zinc contents revealed the accumulation capacity of pot marigold in potting conditions. That TF value >1 in Zn treatments up to 500 mg kg−1 points to the efficiency limit of pot marigold as a Zn-accumulator plant.

Modified screening method of middle american dry bean genotypes reveals new genomic regions on Pv10 associated with anthracnose resistance.

Anthracnose, caused by the fungal pathogen Colletotrichum lindemuthianum (Sacc. & Magnus) Lams.-Scrib., is one of the most devastating diseases in dry bean (Phaseolus vulgaris L.) with seed yield losses up to 100%. Most anthracnose resistance genes thus far identified behave in a dominant manner and were identified by seedling screening. The Middle American Diversity Panel (MDP; n=266) was screened with a modified greenhouse screening method to evaluate the response to anthracnose race 73. Thirty MDP genotypes exhibited resistance to the race of which 16 genotypes were not known to contain anthracnose resistance genes to race 73. GWAS with ~93,000 SNP markers identified four genomic regions, two each on Pv01 and Pv10, associated race 73 resistance. A likelihood-ratio-based R2 analysis indicated the peak four SNP markers are responsible for 26% of the observed phenotypic variation, where one SNP, S10_072250, explains 23% of the total variation. SNP S10_072250 is associated with a new region of anthracnose resistance and is in an intron of a ZPR1-like gene. Further greenhouse testing of the 16 resistant lines without previously known resistance to race 73 revealed various levels of resistance under various levels of disease pressure. Disease resistance was further characterized in the field using four representative genotypes. GTS-900 and Remington exhibited field resistance while Merlot and Maverick were susceptible. Field testing with two different fungicide regimes revealed the resistant genotypes had no significant disease differences. The results suggest resistance to anthracnose may differ at various growth stages and that breeders have been selecting for major genes at early seedling stages while ignoring the effect of alternative genes that may be active at later stages. The newly identified resistant lines may be related to Age Related Resistance (ARR) and could be exploited as parental sources of anthracnose resistance in addition to already known major genes. The physical localization of the multiple regions of resistance confirms the presence of two clusters of disease resistance genes on Pv01 and identifies two new regions of anthracnose resistance on Pv10 possibly associated with ARR. Future research should look at the mode of inheritance of this resistance and its effect when combined with other anthracnose resistance loci.

Preliminary screening of sorghum (Sorghum bicolor L.) germplasm for salinity stress tolerance at the early seedling stage

Preliminary screening of sorghum (Sorghum bicolor L.) germplasm for salinity stress tolerance at the early seedling stage

Morpho-physiological Responses of Finger Millet Genotypes to PEG-Induced Osmotic Stress at an Early Seedling Stage

Morpho-physiological Responses of Finger Millet Genotypes to PEG-Induced Osmotic Stress at an Early Seedling Stage

Fluoride induces morphological change in Fenugreek (Trigonella foenum-graecum L) during germination and early seedling

The goal of this study was to assess the toxicological effects of exposing popular variety of fenugreek [Trigonella foenum-graecum L.] to fluoride during the germination and early seedling stages. In January 2022, the experiment was carried out at Arunachal University of Studies, Namsai. Surface sterilized seeds of uniform size were set for germination in petri-plates over cotton beds, treated with fluoride solutions of varied strengths (0.0, 2.0, 4.0 and 6.0 mM), made from a stock solution of sodium fluoride (50.0mM). The results showed that fluoride might cause considerable phytotoxicity during germination and seedling growth. Fluoride administration resulted 13 to 43.10% suppression of germination from 2.0mM to 6.0mM. Similar results were also found in germination index, germination energy, relative germination rate and seedling vigor index which were significantly reduced by 43.1, 43.1, 34.0, and 84.0% in 150.0 mM, respectively. Fluoride also had significant detrimental impact on seedling length (71.78%) in the intact seven days with increasing doses of fluoride.

Fluoride induces morphological change in Fenugreek (Trigonella foenum-graecum L) during germination and early seedling

The goal of this study was to assess the toxicological effects of exposing popular variety of fenugreek [Trigonella foenum-graecum L.] to fluoride during the germination and early seedling stages. In January 2022, the experiment was carried out at Arunachal University of Studies, Namsai. Surface sterilized seeds of uniform size were set for germination in petri-plates over cotton beds, treated with fluoride solutions of varied strengths (0.0, 2.0, 4.0 and 6.0 mM), made from a stock solution of sodium fluoride (50.0mM). The results showed that fluoride might cause considerable phytotoxicity during germination and seedling growth. Fluoride administration resulted 13 to 43.10% suppression of germination from 2.0mM to 6.0mM. Similar results were also found in germination index, germination energy, relative germination rate and seedling vigor index which were significantly reduced by 43.1, 43.1, 34.0, and 84.0% in 150.0 mM, respectively. Fluoride also had significant detrimental impact on seedling length (71.78%) in the intact seven days with increasing doses of fluoride.

IDENTIFICATION OF DROUGHT-TOLERANT KABULI CHICKPEA (CICER ARIETINUM L.) GENOTYPES AT THE EARLY SEEDLING STAGE

Chickpea (Cicer arietinum L.) is negatively affected by drought stress at all the growth stages, including germination and seedling emergence. The recent study aimed to investigate the drought-tolerant Kabuli chickpea genotypes at the seedling stage under osmotic stress conditions. An in vitro screening technique evaluated the 120 different genotypes of Kabuli chickpea for drought tolerance by using different concentrations of polyethylene glycol (8000) solution, viz., T0: Control, T1: -0.19 MPa, and T2: -0.47 MPa, arranged in a completely randomized design. Data were recorded on germination percentage, root length, shoot length, seedling length, root/shoot ratio, dry matter, seedling vigor index, and proline content. The results of a pooled analysis of variance revealed significant variability among genotypes, different polyethylene glycol treatments, as well as, between genotypes and treatments. The increase of polyethylene glycol levels negatively affected most of the parameters under study, except for proline content, which increased with an increase in polyethylene glycol concentration. The study results indicated that the advanced lines, 15KCC-106, 13KCC-114, 6KCC-103, GP-37, FS-10, and 12KCC-106, performed better under different osmotic stress conditions and gained selection as drought-tolerant advanced lines at early seedling stage. These advanced lines suitably combine with their desirable traits to cope with the drought condition and can serve as a baseline for the improvement of Kabuli chickpea breeding material for drought tolerance.

Screening of Finger Millet (<em>Eleusine coracana</em>) Germplasm for the Blast Disease-Resistance in Sri Lanka

Finger millet is the third important cereal crop in Sri Lanka, mostly cultivated in rainfed uplands in dry and intermediate zones. Blast disease (leaf, finger, and neck blast) caused by Magnaporthe grisea is economically devastating to rice and finger millet worldwide. Host resistance is the most viable option for managing the disease as it is mainly grown in low-input systems. One hundred thirty-nine finger millet accessions collected from the Plant Genetic Resources Center in Sri Lanka were field screened for leaf blast disease in three seasons under artificial inoculation at the Field Crops Research and Development Institute at Mahailluppallama, Sri Lanka. The results revealed that almost all the tested accessions were susceptible to leaf blast at the early seedling stage. However, 5–6 weeks after sowing, the severity of leaf blast disease decreased drastically, and all the germplasm were resistant or moderately resistant. The finger blast and neck blast severity, scored under natural infection, was low under both high and low-density planting. Seven and 87 finger millet accessions consistently showed immune reactions for finger blast and neck blast, respectively. A negative correlation was observed between the days to flowering and blast disease. The early flowering accessions, namely, Ac12968 and Ac2384 from Jaffna, TVFM, 13-1, TVFM-04, and TVFM-02 from Killinochchi, showed a higher severity score for leaf, finger, and neck blast compared to other accessions. A significant positive correlation (P<0.05) was found between finger blast and neck blast, however, these two diseases did not show a significant correlation (P>0.05) with leaf blast.

Genetic factors underlying anaerobic germination in rice: Genome-wide association study and transcriptomic analysis.

The success of rice (Oryza sativa L.) germination and survival under submerged conditions is mainly determined by the rapid growth of the coleoptile to reach the water surface. Previous reports have shown the presence of genetic variability within rice accessions in the levels of flooding tolerance during germination or anaerobic germination (AG). Although many studies have focused on the physiological mechanisms of oxygen stress, few studies have explored the breadth of natural variation in AG tolerance-related traits in rice. In this study, we evaluated the coleoptile lengths of a geographically diverse rice panel of 241 accessions, including global accessions along with elite breeding lines and released cultivars from the United States, under the normal and flooded conditions in laboratory and greenhouse environments. A genome-wide association study (GWAS) was performed using a 7K single-nucleotide polymorphism (SNP) array and the phenotypic data of normal coleoptile length, flooded coleoptile length, flooding tolerance index, and survival at 14 d after seeding (DAS). Out of the 30 significant GWAS quantitative trait loci (QTL) regions identified, 14 colocalized with previously identified candidate genes of AG tolerance, whereas 16 were potentially novel. Two rice accessions showing contrasting phenotypic responses to AG stress were selected for the transcriptomics study. The combined approach of GWAS and transcriptomics analysis identified 77 potential candidate genes related to AG tolerance. The findings of our study may assist rice improvement programs in developing rice cultivars with robust tolerance under flooding stress during germination and the early seedling stage.

Fungal endophytes from salt-adapted plants confer salt tolerance and promote growth in wheat (Triticum aestivum L.) at early seedling stage.

With increasing human global population, increased yield under saline conditions is a desirable trait for major food crops. Use of endophytes, isolated from halophytic hosts, seems to be an exciting approach for conferring salt tolerance to a salt-sensitive crop. Therefore, in the current study, fungal endophytes were isolated from halophytic plants' roots and their ability to withstand in vitro salt stress was evaluated. The fungal endophytes could withstand up to 1M NaCl concentrations and this tolerance was independent of their host or tissue source. When inoculated on salt-sensitive wheat seeds/seedlings, several of the endophytes showed a positive impact on germination and biomass-related parameters upon salt stress, both in vitro and under glasshouse conditions. One of the isolates from dicot plants (identified as Microsphaeropsis arundinis) could successfully colonize wheat and promote its growth under salt and no-salt conditions. Amongst the fungal isolates that are known to be natural endophytes of wheat, Chaetomium globosum was the best performing isolate and has previously been reported to be an effective biocontrol agent. Based on the results of our preliminary study, we suggest that these fungal endophytes could prove beneficial for enhancing the salt stress tolerance of wheat crop.

Combined transcriptome and metabolome reveal glutathione metabolism plays a critical role in resistance to salinity in rice landraces HD961.

Rice (Oryza sativa) is one of the most important food crops around the world, which is sensitive to salt stress, especially in the seedling and booting stage. HD961 is a salt-tolerant rice landrace that grows along coastal beaches and has disease and insect pest resistance, salt tolerance, and vigorous growth characteristics. We performed a combined transcriptome and metabolome analysis to clarify salinity resistance mechanisms in cultivar HD961, which has adapted to salinity soil at the early seedling stage. The results showed that the growth and antioxidant capacity of HD961 were stronger than 9311 under salt stress (SS). Transcriptomic analysis showed that a total of 6,145, 3,309, 1,819, and 1,296 differentially expressed genes (DEGs) were identified in the groups of TH60 (control group vs. 60 mM group of HD961 for transcriptome), TH120 (control group vs. 120 mM group of HD961 for transcriptome), T60 (control group vs. 60 mM group of 9311 for transcriptome), and T120 (control group vs. 120 mM group of 9311 for transcriptome), respectively. Starch and sucrose metabolism and phenylpropanoid biosynthesis were shared in the four treatment groups based on a KEGG enrichment analysis of DEGs. In addition, alpha-linolenic acid metabolism, plant hormone signal transduction, plant-pathogen interaction, and fatty acid elongation were specific and significantly different in HD961. A total of 92, 158, 151, and 179 significantly regulated metabolites (SRMs) responded to SS in MH60 (control group vs. 60 mM group of HD961 for metabolome), MH120 (control group vs. 120 mM group of HD961 for metabolome), M60 (control group vs. 60 mM group of 9311 for metabolome), and M120 (control group vs. 120 mM group of 9311 for metabolome), respectively. The KEGG analysis showed that eight common metabolic pathways were identified in the four treatment groups, of which biosynthesis of amino acids was the most significant. Three specific metabolic pathways were identified in the HD961, including glutathione metabolism, ascorbate and aldarate metabolism, and pantothenate and CoA biosynthesis. Integrative analysis between the transcriptome and metabolome showed that glutathione metabolism was specific and significantly affected under SS in HD961. A total of seven SRMs and 48 DEGs and four SRMs and 15 DEGs were identified in the glutathione metabolism pathway in HD961 and 9311, respectively. The Pearson correlation analysis showed a significant correlation between reduced glutathione and 16 genes (12 upregulated and four downregulated genes), suggesting these genes could be candidates as salt-tolerance regulation genes. Collectively, our data show that glutathione metabolism plays a critical role in response to SS in rice. Moreover, the stronger regulative ability of related common genes and metabolites might contribute to salt resistance in HD961.

Improvement of Salinity Tolerance in Rice Seedlings by Exogenous Magnesium Sulfate Application

This study was conducted to develop the salt tolerance of rice by exogenous application of magnesium sulfate supplement (MgSO4). The salinization was carried out on 7-day-old rice seedlings including BC15 (salinity tolerant) and DT84DB (salinity susceptible) varieties with 0.5 mM MgSO4. The exogenous application of MgSO4 significantly improves the growth of seedlings of both varieties. In addition, antioxidant activities increase in line with the raise of total phenolic and total flavonoid contents. Remarkably, the contents of momilactone B (MB) and phenolic compounds including tricin, ρ-coumaric, salicylic, cinnamic, benzoic, and ferulic acids simultaneously rise in both varieties treated by salinity and 0.5 mM MgSO4. Interestingly, MB was not found in the salt-treated samples but presents with considerable contents in the salt and MgSO4-treated cultivars. The findings imply that MgSO4 may significantly improve the salt tolerance of rice seedlings through the enhancement of secondary metabolic synthesis pathways, of which phenolic acids and momilactone B may play a crucial role in the response of rice to salt stress. In contrast, momilactone A (MA) did not show any contribution in salinity tolerance of examined rice cultivars at the early seedling stage. Further investigations on the effect of MgSO4 exogenous application in improving salinity tolerance of various rice varieties at other growing stages should be carried out.

An optimized index for cold tolerance assessment in rice during germination and early seedling stage

An optimized index for cold tolerance assessment in rice during germination and early seedling stage

Morpho-physiological response of groundnut genotypes to salinity stress at early seedling stage

An experiment was conducted at the Department of Crop Botany, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh during the period from January, 2020 to June, 2020 to evaluate the morpho-physiological response of groundnut genotypes under salinity stress at early seedling stage. The four groundnut genotypes were, namely, V1= BARI Chinabadam-8, V2= Maijchar badam (Dhaka-1), V3= Binachinabadam-6 and V4= Binachinabadam-8. Two salinity levels were, namely, S1= 0 dS/m NaCl and S2= 12 dS/m NaCl used for hydroponic experiment. The treatments were designed in a Completely Randomized Design (CRD) with four replications in a factorial arrangement. The parameters of the experiment measured were root length, shoot length, number of leaves, number of leaflets, leaf area, Soil Plant Analysis Development (SPAD) value, root fresh weight, leaf fresh weight, shoot fresh weight, root dry weight, leaf dry weight, and shoot dry weight. The result indicated that there were significant differences between the genotypes and salinity stress in all of the studied parameters. Maximum root length (33.20 cm), number of leaves (7.25), number of leaflet (24.50), leaf area (128.07 cm2), SPAD value (41.67), leaf fresh weight (3.43 g) and shoot dry weight (0.11 g) were recorded in the genotype Binachinabadam-6 in a combination of control condition. On the contrary the minimum shoot length (12.17 cm), root length (16.72 cm), number of leaves (2.75), number of leaflet (15.50), leaf area (21.90 cm2), SPAD value (31.42), leaf fresh weight (0.72 g), shoot fresh weight (0.92 g), leaf dry weight (0.11 g) and shoot dry weight (0.09 g) were recorded from the genotype Binachinabadam-8 in stress condition. So, the higher susceptible genotype Binachinabadam-8 to saline conditions and the lower was Binachinabadam-6. Therefore, it may be concluded that the variety Binachinabadam-6 will be more suitable for the saline prone areas of Bangladesh.

Salicylic acid: To assuage salinity stress at the early seedling stage of rice

Being sessile, plants rely on internal modulation of growth and development to cope up with the changing environmental condition. Among the endogenous regulators, salicylic acid has a major role in restoration of plant growth and providing tolerance towards diverse environmental stresses including salinity stress. Seven genotypes FL 478, ADT 45, ADT 46, ADT 50, CR 1009, KKL(R)1, IW Ponni ) were selected  and treated with 100 ppm SA and sown under saline condition(150 mM NaCl). In this experiment the growth parameters like root length, shoot length, root to shoot ratio and biochemical parameters like total chlorophyll and carotenoids were estimated. When rice seeds treated with 100 ppm of SA, the inhibitory effect of salinity stress on growth parameters were alleviated and significant increase in root to shoot ratio were noticed, which is a very important physiological trait for abiotic stress tolerance. Moreover, there is an increase in both chlorophyll and carotenoid content in seedling stage. This shows that, the increased growth parameters might have attributed to the increase in photosynthetic pigments by exogenous application of salicylic acid under salinity stress in rice.

Mitigation of the salinity stress in rapeseed (Brassica napus L.) productivity by exogenous applications of bio-selenium nanoparticles during the early seedling stage

In recent years, much attention has been directed toward using nanoparticles (NPs) as one of the most effective strategies to improve plant growth, especially under salt stress conditions. Further research has been conducted to develop NPs using various chemical ways; accordingly, knowledge about the beneficial effect of bioSeNPs in rapeseed is obscure. Selenium (Se) is a vital micronutrient with a series of physiological and antioxidative properties. Seed priming is emerging as a low-cost, efficient, and environment-friendly seed treatment in nanotechnology. The current study was carried out to examine the promising effects of nanopriming via bioSeNPs on the expression level of aquaporin genes, seed microstructure, seed germination, growth traits, physiochemical attributes, and minerals uptake of two rapeseed cultivars under salinity stress conditions. Our investigation monitored the positive effects of bioSeNPs on the expression level of aquaporin genes (BnPIP1-1 and BnPIP2-1) and water uptake during the seed imbibition (4 and 8 h of priming), which indicated higher imbibition potential and germination promotion with bioSeNPs application (most effective at 150 μmol/L). The total performance index was significantly enhanced with nano-treatments in rapeseed seedlings. Collectively, nano-application improved seed microstructure, seed germination, and photosynthetic efficiency directly correlated with higher seedlings biomass, especially with a higher concentration of bioSeNPs. The enhancement in α-amylase and free amino acid contents in nanoprimed seeds resulted in rapid seed germination. Moreover, bioSeNPs increased the osmotic adjustment and enhanced the efficiency of the plant's defense system by improving the activity of enzymatic and non-enzymatic antioxidants, thus enhancing ROS scavenging under salt stress. The obtained results may indicate the strengthening of seed vigor, improving seedling growth and physiochemical attributes via bioSeNPs. Our findings displayed that bioSeNPs modulated the Na+ and K+ uptake, which improved the rapeseed growth and showed a close relationship with the low contents of toxic Na+ ion; thus, it prevented oxidative damage due to salt stress. This comprehensive data can add more knowledge to understand the mechanisms behind plant-bioSeNPs interaction and provide physiological evidence for the beneficial roles of nanopriming using bioSeNPs on rapeseed germination and seedling development under salinity stress conditions. Such studies can be used to develop simple prepackaged nano primer products, which can be used before sowing to boost seed germination and crop productivity under stress conditions.