Early Root Research Articles

SCIENCE, RELIGION, AND CULTURE

SCIENCE, RELIGION, AND CULTURE

Paul J. Polgar. Standard-Bearers of Equality: America’s First Abolition Movement.

Standard-Bearers of Equality: America’s First Abolition Movement challenges successfully historical scholarship that places the antebellum-era abolitionist movement as the critical and only significant period of early antislavery activism. Paul J. Polgar invites readers to evaluate the “first movement of abolitionism” of Middle Atlantic states—namely, Pennsylvania and New York—in order to review the multicultural roots of activism and gradual abolitionist leadership spanning the postrevolutionary and early national periods. His book references the movement’s early roots built upon the Germantown Protest of 1688 and later individual work of many New Jersey and Pennsylvania Quakers, including John Woolman, Anthony Benezet, Benjamin Lay, and David Cooper (4, 44–52). Yet, when quoting Quaker mystic and visionary Woolman, Polgar might have referenced Andrew White’s article, “A ‘Consuming’ Oppression: Sugar, Cannibalism and John Woolman’s 1770 Slave Dream,” (2007) on consumer boycotts advocated by Woolman to boycott sugar and other goods made by slaves that were more radical in nature than those held by the majority of abolitionists of his day and the first movement period (2, 10, 17–18, 23, 24). Historian Julie L. Holcomb dedicated her book, Moral Commerce: Quakers and the Transatlantic Boycott of the Slave Labor Economy (2016), to evaluating the power of a consumer boycott and within it described Woolman’s boycott strategy to combat “evil commerce” as it related to capitalism and the slave industry (31).

Phylogeographic history of mitochondrial haplogroup J in Scandinavia.

Mitochondrial DNA haplogroup J is the third most frequent haplogroup in modern-day Scandinavia, although it did not originate there. To infer the genetic history of haplogroup J in Scandinavia, we examined worldwide mitogenome sequences using a maximum-likelihood phylogenetic approach. Haplogroup J mitogenome sequences were gathered from GenBank (n= 2245) and aligned against the ancestral Reconstructed Sapiens Reference Sequence. We also analyzed haplogroup J Viking Age sequences from the European Nucleotide Archive (n= 54). Genetic distances were estimated from these data and projected onto a maximum likelihood rooted phylogenetic tree to analyze clustering and branching dates. Haplogroup J originated approximately 42.6 kya (95% CI: 30.0-64.7), with several of its earliest branches being found within the Arabian Peninsula and Northern Africa. J1b was found most frequently in the Near East and Arabian Peninsula, while J1c occurred most frequently in Europe. Based on phylogenetic dating, subhaplogroup J1c has its early roots in the Mediterranean and Western Balkans. Otherwise, the majority of the branches found in Scandinavia are younger than those seen elsewhere, indicating that haplogroup J dispersed relatively recently into Northern Europe, most plausibly with Neolithic farmers. Haplogroup J appeared when Scandinavia was transitioning to agriculture over 6 kya, with J1c being the most common lineage there today. Changes in the distribution of haplogroup J mtDNAs were likely driven by the expansion of farming from West Asia into Southern Europe, followed by a later expansion into Scandinavia, with other J subhaplogroups appearing among Scandinavian groups as early as the Viking Age.

Technology and More: What the Internet Owes the UK

Abstract Richard Sharpe, from the Archives of IT, explores the internet’s history and finds that, though being a very international entity today, the global network of networks has its early roots planted firmly in UK soil.

Spatiotemporal and Transcriptional Characterization on Tanshinone Initial Synthesis in Salvia miltiorrhiza Roots.

Tanshinones are the bioactive constituents of Danshen (Salvia miltiorrhiza Bunge), which is used in Traditional Chinese Medicine to treat cardiovascular and other diseases, and they synthesize and accumulate in the root periderm of S. miltiorrhiza. However, there is no relevant report on the initial stage of tanshinone synthesis, as well as the root structure and gene expression characteristics. The present study aims to provide new insights into how these bioactive principles begin to synthesize by characterizing possible differences in their biosynthesis and accumulation during early root development from both spatial and temporal aspects. The morphological characteristics and the content of tanshinones in roots of S. miltiorrhiza were investigated in detail by monitoring the seedlings within 65 days after germination (DAGs). The ONT transcriptome sequencing was applied to investigate gene expression patterns. The periderm of the S. miltiorrhiza storage taproot initially synthesized tanshinone on about 30 DAGs. Three critical stages of tanshinone synthesis were preliminarily determined: preparation, the initial synthesis, and the continuous rapid synthesis. The difference of taproots in the first two stages was the smallest, and the differentially expressed genes (DEGs) were mainly enriched in terpene synthesis. Most genes involved in tanshinone synthesis were up regulated during the gradual formation of the red taproot. Plant hormone signal transduction and ABC transport pathways were widely involved in S. miltiorrhiza taproot development. Five candidate genes that may participate in or regulate tanshinone synthesis were screened according to the co-expression pattern. Moreover, photosynthetic ferredoxin (FD), cytochrome P450 reductase (CPR), and CCAAT binding transcription factor (CBF) were predicted to interact with the known downstream essential enzyme genes directly. The above results provide a necessary basis for analyzing the initial synthesis and regulation mechanism of Tanshinones.

Moulding democratic citizens: democracy and education in modern European history – an introduction

ABSTRACT Politicians and intellectuals across Europe largely agree that democracies require educated citizens. However, current conflicts surrounding disinformation on social media, right-wing populism and religious fundamentalism seem to cast doubts on the democratic maturity of Western societies. At the same time, controversies exist around the thorny issue of what kind of education befits democracy and whether and how governments should intervene in the political education of their citizens. These controversies have a long and complicated history, which stands at the centre of this special issue. By both exploring how states, political parties and social movements tried to shape political identities and assessing the resulting successes, failures and contradictions of these attempts, the articles provide a unique window into the conflicted history of West European democracy. Educational programmes attempted not only to empower individuals, but also to manage, control and frame political and civic engagement. While these schemes also always entailed the promise of equality, in practice, they often identified certain social groups – such as women, the working classes or immigrants – who ostensibly lacked the moral or cognitive preconditions for democratic citizenship and therefore needed special educational attention. The individuals to be educated did not passively give in to the competing programmes of governments and political movements. Rather, they often circumvented or even openly resisted educational schemes from above that sought to change their lifestyles and political consciousness. In order to place the articles in this special issue in a wider historiographical context, this introduction illuminates the nineteenth and early twentieth-century roots of the tensions that haunted the endeavours to educate post-1945 democratic citizens.

Cell-specific pathways recruited for symbiotic nodulation in the Medicago truncatula legume

Medicago truncatula is a model legume species that has been studied for decades to understand the symbiotic relationship between legumes and soil bacteria collectively named rhizobia. This symbiosis called nodulation is initiated in roots with the infection of root hair cells by the bacteria, as well as the initiation of nodule primordia from root cortical, endodermal, and pericycle cells, leading to the development of a new root organ, the nodule, where bacteria fix and assimilate the atmospheric dinitrogen for the benefit of the plant. Here, we report the isolation and use of the nuclei from mock and rhizobia-inoculated roots for the single nuclei RNA-seq (sNucRNA-seq) profiling to gain a deeper understanding of early responses to rhizobial infection in Medicago roots. A gene expression map of the Medicago root was generated, comprising 25 clusters, which were annotated as specific cell types using 119 Medicago marker genes and orthologs to Arabidopsis cell-type marker genes. A focus on root hair, cortex, endodermis, and pericycle cell types, showing the strongest differential regulation in response to a short-term (48 h) rhizobium inoculation, revealed not only known genes and functional pathways, validating the sNucRNA-seq approach, but also numerous novel genes and pathways, allowing a comprehensive analysis of early root symbiotic responses at a cell type-specific level.

QTL mapping for early root and shoot vigor of upland rice (Oryza sativa L.) under P deficient field conditions in Japan and Madagascar.

Upland rice production is limited by the low phosphorus (P) availability of many highly weathered tropical soils and P deficiency is likely to become increasingly limiting in future drier climates because P mobility decreases sharply with soil moisture. Good seedling root development will be crucial to cope with the combined effects of low P and water availability. Upland rice genebank accession DJ123 was used as a donor for P efficiency and root vigor traits in a cross with inefficient local variety Nerica4 and a set of backcross lines were used to characterize the seedling stage response of upland rice to low P availability and to identify associated QTL in field trials in Japan and Madagascar. Ten QTL were detected for crown root number, root, shoot and total dry weight per plant in a highly P deficient field in Japan using the BC1F3 generation. Of these, qPef9 on chromosome 9 affected multiple traits, increasing root number, root weight and total biomass, whereas a neighboring QTL on chromosome 9 (qPef9-2) increased shoot biomass. Field trials with derived BC1F5 lines in a low-P field in Madagascar confirmed a highly influential region on chromosome 9. However, qPef9-2 appeared more influential than qPef9, as the shoot and root biomass contrast between lines carrying DJ123 or Nerica4 alleles at qPef9-2 was +23.8% and +13.5% compared to +19.2% and +14.4% at qPef9. This advantage increased further during the growing season, leading to 46% higher shoot biomass at the late vegetative stage. Results suggest an introgression between 8.0 and 12.9 Mb on chromosome 9 from P efficient donor DJ123 can improve plant performance under P-limited conditions. The QTL identified here have practical relevance because they were confirmed in the target genetic background of the local variety Nerica4 and can therefore be applied directly to improve its performance.

Medical Records: A Historical Narrative.

The history of medical records is thousand-year-long, with earlier roots in ancient civilizations. Until the 19th century, medical records mainly served educational purposes, later assuming other roles such as in insurance or legal procedures. This article comprehensively describes and reviews the development of medical records from ancient to modern times in Europe and North America, reflecting alterations and adaptations compliant with the mental and technological capabilities of a given period. We searched PubMed and Google Scholar databases to collect pertinent articles. English articles or those having English abstracts were considered. The search terms included “Medical Records,” “Health Records,” “History of Medicine,” and “eHealth” and covered the last hundred years. References were also picked out from the identified articles. Overall, 600 articles were identified, 158 of which were judged thematically relevant. The general conclusion is that medical records undergo a revolutionary change from paper-based to electronic format, which reflects the development of eHealth systems. The migration process to eHealth records involves the use of artificial intelligence (AI) algorithms that streamline medical services by using faster and simpler working methods. AI benefits both patients and providers as it improves patient management and communication among medical centers, spares resources, identifies contamination or infections, and limits health costs. These advantages have become pointedly apparent during the recent COVID-19 scourge.

The Relationship of Art Therapy to Spirituality

Art therapy is based on the idea that creative artistic processes facilitate repair and healing and is a form of psychotherapy where image creation and object usage are the primary forms of expression and communication. Although art therapy’s emergence as a profession is relatively recent, the roots of using art for healing are as old as the symbolic rituals ancient cultures used in religious ceremonies. Art therapy also spread universally, from mummy’s ornaments in Egypt to Tibetan monks’ sand mandalas. The ancient intertwined history of the relationship between art and spirituality as well as between spirituality and healing has aroused curiosity about the nature of the relationship between art therapy and spirituality. Based on this curiosity, the article will address within its scope the definition of art therapy and its early roots, followed by discussion on the relationship between art therapy and spirituality with regard to six propositions. By considering the close old friendship art has with therapy and spirituality in the context of art therapy, the study can contribute to the currently relevant literature on both art therapy and spirituality, as well as to the theoretical framework of spiritually oriented practices in art therapy.

Reflections, research, and implications of decades of activism by educators to create a movement to address sexual harassment in K‐12 schools in the United States

AbstractAn organized response to sexual harassment (SH) in K‐12 schools in the US traces its development as a social movement to the larger women's rights movement in the late 1970s. It was an outgrowth of the social movement of feminist activists who protested and filed lawsuits to draw attention to SH in the workplace to gain recognition for the problem as one of equity for working women. The focus on SH in K‐12 schools did not begin as an academic pursuit or with an emphasis on research – rather shares its origins as an activist movement to rectify injustices. Authors document the unwritten history of this social movement, by examining the early roots to address and prevent SH in K‐12 schools. The authors review survey and intervention research from the initial movement through the past couple of decades, noting limitations and challenges for future efforts to prevent and eliminate SH in K‐12 schools. The role of youth activism in advancing the movement against SH in schools represents hope of a renewal of activism with a robust gendered perspective. Authors provide next steps for research and action.

Inbreeding depression changes with stress response over time in flooded Mimulus guttatus

Inbreeding usually reduces offspring fitness (‘inbreeding depression’, ID), and may affect the plasticity of functional traits involved in the response to stress. ID is often found to increase under stress, but there are also reports of no effects or even a reduction of ID under stress. One reason for this variation that has received little attention may be related to different concepts of stress. In particular, the magnitude of ID may be unrelated to the effect of an environment on fitness (evolutionary stress concept), but increase particularly during the ‘alarm phase’ after a stress has been initiated (physiological stress concept). We clonally replicated inbred and outbred Mimulus guttatus plants, for which ID was known to increase under flooding. We exposed the clonal replicates to control and flooding conditions and harvested replicates of each genotype after two, six and 11 weeks of growth. As functional traits related to stress response we measured chlorophyll fluorescence, root mass and the production of stolons and adventitious roots. As fitness estimates we measured biomass and flower number, and we pollinated a subset of plants and grew a second generation of plants under control and flooding conditions to calculate multiplicative fitness. Overall, M. guttatus proved to be very flooding-tolerant. Chlorophyll fluorescence (Fv/Fm) was not influenced by flooding, but decreased with leaf age and increased after fertilization. At the end of the experiment, biomass and flower number (F1 generation) as well as multiplicative fitness (including performance in the F2 generation) were even higher under flooding than under control conditions. Flooding reduced the root mass in the pots, but increased the production of stolons and floating roots. Plasticity in these traits can be regarded as beneficial, although selection gradient analysis failed to identify plasticity in stolon number as adaptive. Only two functional traits were influenced by an interaction between flooding and inbreeding, early stolon length (suggesting a reduced flooding escape response of inbred plants) and root tissue density of floating adventitious roots (suggesting a reduced aeration of the roots of inbred offspring). ID in fitness-related traits was higher under flooding, but its magnitude changed strongly over the course of the experiment. ID under flooding was particularly high after two weeks (δ = 0.42 vs. 0.05 in the control), suggesting sensitivity of inbred plants to the initiation of flooding (‘alarm phase’ of stress response). This effect had disappeared after 6 weeks when plants had acclimated to ongoing flooding. However, under flooding ID increased again after 11 weeks, this time because outbred plants grew much better under flooded than control conditions, and the same pattern was found for the multiplicative fitness function (δ = 0.68 under flooding vs. 0.36 in the control). Our results suggest that ID was higher under flooding, but not because this environment was generally more stressful. Instead, at an early stage ID increased because inbred offspring was more sensitive to the physiological stress after the initiation of flooding (‘alarm phase’), whereas at a late stage ID increased because outbred offspring was more capable of exploiting the favourable flooding conditions. In general, our results show that phenotypic plasticity may often be robust against the effects of inbreeding. Moreover, ID may increase in particular under conditions of physiological stress, during which many stress-specific genes are expressed, whereas ID may not necessarily increase under constant poor conditions that reduce fitness, even if the stress is very strong in the evolutionary sense.

Integrating multi-locus genome-wide association studies with transcriptomic data to identify genetic loci underlying adult root trait responses to drought stress in flax (Linum usitatissimum L.)

Water stress is a major constraint on crop production and, root systems are one of the key features of plants determining drought tolerance and yield. In this study, five root morphological traits and their stability indices were evaluated at the reproductive stage on an association panel consisting of 120 diverse Linum usitatissimum accessions grown under well-watered and water-limiting conditions. To identify quantitative trait loci (QTL) and candidate genes positively influencing root traits under drought, combined multi-locus genome-wide association studies (ML-GWAS) and transcriptomic data analysis were carried out. A total of 112 QTL were identified accounting for 0.45–24.28 % of the phenotypic variation for root-related traits. Fifty QTL were detected by at least two ML-GWAS models, and 26 were co-located with previously reported QTL associated with yield-related traits under drought and early root and shoot development. Phenotypic differences between two contrasting subsets of accessions for the traits assessed were attributable to the accumulation of positive QTL alleles (PQTL), where the subset with the largest number of PQTL registered 154 % and 69 % greater total root length and yield under drought stress, respectively, than the subset with the fewest PQTL. Transcriptomic data analysis identified 766 differentially expressed genes (DEGs) responsive to drought stress in 108 QTL. Annotation of the 766 DEGs yielded functional genes involved in root development, stomatal closure, ROS (reactive oxygen species) scavenging, and hormone responses, among others. Taken together, the combined use of GWAS and transcriptomic data analyses provide the foundation for our enhanced understanding of root trait morphological changes in response to drought stress, and to achieve superior yield resilience/stability under water shortages in flax through enhanced molecular breeding. All data supporting the findings of this study are available within the paper and within its Supplementary data • Large genetic variation observed for adult root traits under drought and irrigated conditions. • Root traits explained 25–32 % of yield variation under drought stress. • A total of 112 QTL were identified for root traits and their stability indices in flax. • Twenty-six QTL co-located with previously reported QTL for yield traits under drought and root and shoot development. • Transcriptomic data analysis identified 766 drought responsive differentially expressed genes within the LD blocks of 108 significant QTL.

GA signaling expands: The plant UBX domain-containing protein 1 is a binding partner for the GA receptor.

The plant Ubiquitin Regulatory X (UBX) domain-containing protein 1 (PUX1) functions as a negative regulator of gibberellin (GA) signaling. GAs are plant hormones that stimulate seed germination, the transition to flowering, and cell elongation and division. Loss of Arabidopsis (Arabidopsis thaliana) PUX1 resulted in a "GA-overdose" phenotype including early flowering, increased stem and root elongation, and partial resistance to the GA-biosynthesis inhibitor paclobutrazol during seed germination and root elongation. Furthermore, GA application failed to stimulate further stem elongation or flowering onset suggesting that elongation and flowering response to GA had reached its maximum. GA hormone partially repressed PUX1 protein accumulation, and PUX1 showed a GA-independent interaction with the GA receptor GA-INSENSITIVE DWARF-1 (GID1). This suggests that PUX1 is GA regulated and/or regulates elements of the GA signaling pathway. Consistent with PUX1 function as a negative regulator of GA signaling, the pux1 mutant caused increased GID1 expression and decreased accumulation of the DELLA REPRESSOR OF GA1-3, RGA. PUX1 is a negative regulator of the hexameric AAA+ ATPase CDC48, a protein that functions in diverse cellular processes including unfolding proteins in preparation for proteasomal degradation, cell division, and expansion. PUX1 binding to GID1 required the UBX domain, a binding motif necessary for CDC48 interaction. Moreover, PUX1 overexpression in cell culture not only stimulated the disassembly of CDC48 hexamer but also resulted in co-fractionation of GID1, PUX1, and CDC48 subunits in velocity sedimentation assays. Based on our results, we propose that PUX1 and CDC48 are additional factors that need to be incorporated into our understanding of GA signaling.

Impact of elevated temperature on root traits and microbial interaction in cotton (Gossypium hirsutum L.) genotypes

Climate change mainly alters the plant phyllosphere and rhizosphere resource allocations. Compared with shoot parameters, there is less information about how roots, especially root system architecture (RSA) and their interactions with others, may respond to elevated temperature changes. These responses could greatly influence different species acquisition of resources and their competition with their neighbours. The main aim of this experiment was to evaluate the effects of ambient temperature (T1) and elevated temperature (+4oC) (T2) in Open-top chamber (OTC) on root traits and microbial interaction changes in cotton (Gossypium hirsutum L.). A pot experiment was conducted at the Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, during 2020-2021 to investigate the root traits and microbial interactions. Cotton varieties, namely KC3, SVPR6, TSH325, TSH357 and TSH375 were screened at the seedling level for cellular thermo tolerance and further, at the root level, these selected varieties were studied against the elevated temperature condition for 10 days in OTC during the stage of flowering to boll development period along with control temperature condition. Root interactions' intensity and direction may fluctuate as a result of variations in RSA responses between species. Negative root interactions could become more intense under high temperature circumstances and species with bigger roots and greater early root growth had stronger competitive advantages. The present findings showed that elevated temperatures promote various microbial growths in the geothermal regions, enhancing the root angle and root length of cotton species. Among the genotypes, KC3 and SVPR6 performed better under elevated temperatures.

The Physiological and Biochemical Response of Field Bean (Vicia faba L. (partim)) to Electromagnetic Field Exposure Is Influenced by Seed Age, Light Conditions, and Growth Media

Research interest into the exposure of plants to magnetic fields (MF), including electromagnetic fields (EMF), has increased recently but results often vary depending on factors such as plant species and treatment dose. In this study, we exposed young (one year) and old (four years) field bean (Vicia faba L. (partim)) seeds to EMF (50 Hz, 7 mT) and observed seed germination and seedling growth under different conditions (growth media and light). The results indicated a stimulation by EMF of germination and early root growth of Petri dish-sown old seeds in continuous darkness and inhibition of germination of the pot-sown young seeds under long-day conditions. Root growth of two-week-old seedlings from pot-sown young seeds was stimulated by EMF treatment while their stem growth was inhibited. Some selected biochemical traits were examined, showing specific changes in membrane integrity, amylase activity, H2O2 levels, photosynthetic pigments, and content of the main groups of phytohormones, depending on seed age. The results indicate that priming of field bean seeds with EMF (50 Hz, 7 mT) could be a eustress factor that influences germination, early growth, and cellular activities and could positively influence the ability of field bean plants to grow and develop in more stressful conditions at later stages.

Outcomes after ROSS procedure with pulmonary autograft reinforcement using reimplantation technique

Pulmonary autograft reinforcement to prevent dilatation and subsequent neo-aortic valve regurgitation was reported. However, the late functional outcome of the native pulmonary valve inside a rigid Dacron conduit requires to be documented. We aimed to assess the results of modified Ross procedure associated with autograft reinforcement reimplantation technique. Outcomes of 61 consecutive patients who underwent Ross procedure with reinforcement between 2009 and 2021 were analyzed. Majority of cases has presented with mono or bicuspid aortic valve ( n = 52; 85%), predominant aortic valve regurgitation ( n = 47; 77%) and dilatation (> 30 mm) of the ascending aorta ( n = 33; 54%). Forty-seven patients (77%) had prior aortic valve procedure including 38 surgical repair (62%) and 9 balloon dilatation (15%). Pulmonary autograft was reimplanted in a Dacron conduit of a median diameter = 25.6 mm (ranges: 20–30) using Tirone David's valve sparing aortic root replacement technique. No death occurred. Median age at procedure was 16.8 years (range: 6–38). Two patients (3%) required early neo-Aortic root revision, one other was reoperated later on resulting in neo-aortic valve replacement in 3 (4.9% IC 95% [0.34%; 12.7%]) because of respectively infective endocarditis, left ventricular false aneurysm and leaflet perforation. Six patients required right ventricular outflow conduit replacement including one percutaneous replacement. At mean 66 ± 50.5 months postoperatively, the survival rate with freedom from reintervention was 83% [71.9; 93.5] and the deterioration of the initial neo-Aortic valve function (regurgitation or stenosis) was not observed ( Fig. 1 ). Autograft reinforcement by means of reimplantation technique allowed to extend the indications for Ross procedure to all patients requiring Aortic valve replacement and prevented neo-Aortic root dilatation. Failures were early and rare, and late controls confirmed the stability of the neo-aortic valve function in follow-up.

Inflammatory Status of Excavated Pulp Tissue and Internal Root Resorption in Pulpotomized Primary Molars.

Internal root resorption after pulpotomy is a pathological phenomenon and can lead to early root resorption and subsequent loss of the tooth. To assess the relationship between initial inflammatory coronal pulp status in decayed primary molars treated by pulpotomy and internal root resorption after one-year follow-up. In this clinical in vivo and in vitro experiment, vital pulpotomies were performed on 50 primary molars from 50 patients aged 5 to 10 years. Coronal pulp was carefully removed followed by hemostasis and placement of a reinforced zinc oxide eugenol over the vital radicular pulp. Enzyme-linked immunosorbent assay (ELISA) assay was done on coronal pulp samples and the level of tumor necrosis factor-alpha (TNF- α) and interlukin-6 (IL-6) was measured. After a 12-month follow-up, periapical radiographs were taken from pulpotomized teeth. Kolmogorov-Smirnov, Chi-square, Kruskal-Wallis, and Mann-Whitney tests were implemented. 11 treated teeth (22%) showed an internal root resorption as diagnosed on X-rays. No significant association was found between TNF- α, IL-6 levels, and pathological root resorption respectively (p= 0.953) and (p= 0.944). A significant association between age and pathological root resorption was observed (p= 0.031). No significant association between remaining dentin thickness and pathological root resorption was established (p= 0.346). There was no association between pro-inflammatory cytokines levels/ TNF-α, IL-6 and internal root resorption following pulpotomy in pediatric patients.

The Legal Ethics in Financial Technology: How is it Regulated?

The FinTech sector in ASEAN has grown dramatically. Southeast Asian FinTechs like GoJek and Grab grew from early roots to become digital economy behemoths. New players enter the fray on a regular basis, drawn in by their success and the region's market potential for the people. The purpose of this research is to find out the regulations and ethics of financial law that are used for the welfare of the people. The approach method used was a normative juridical approach, the results of the research result state that: Regulations or laws provide a level of trust, security, and comfort for the community and are something that the state must do without exception as a state of law. One form of protection is the establishment of laws or regulations relating to lending activities, specifically to protect users' rights. To ensure that this process is successful, the state intervenes in the contractual relationship between consumers and business actors, aiming to create a balanced relationship between users and business actors. Funder protection is concerned with protection. Law provided in an effort to obtain goods and services from potential losses due to their use.

Nutrition and Health in Human Evolution-Past to Present.

Anyone who wants to understand the biological nature of humans and their special characteristics must look far back into evolutionary history. Today’s way of life is drastically different from that of our ancestors. For almost 99% of human history, gathering and hunting have been the basis of nutrition. It was not until about 12,000 years ago that humans began domesticating plants and animals. Bioarchaeologically and biochemically, this can be traced back to our earliest roots. Modern living conditions and the quality of human life are better today than ever before. However, neither physically nor psychosocially have we made this adjustment and we are paying a high health price for it. The studies presented allow us to reconstruct food supply, lifestyles, and dietary habits: from the earliest primates, through hunter-gatherers of the Paleolithic, farming communities since the beginning of the Anthropocene, to the Industrial Age and the present. The comprehensive data pool allows extraction of all findings of medical relevance. Our recent lifestyle and diet are essentially determined by our culture rather than by our millions of years of ancestry. Culture is permanently in a dominant position compared to natural evolution. Thereby culture does not form a contrast to nature but represents its result. There is no doubt that we are biologically adapted to culture, but it is questionable how much culture humans can cope with.