Background After quickly spreading since March 2009 in Mexico, influenza pandemic H1N1 has affected a large part of the world’s population. Countries have made great efforts to contain the pandemic. An important key in containing community transmission and reducing the impacts of the pandemic influenza is to have local people educated to have good knowledge, attitude and practice toward influenza pandemic (H1N1). The aim of this study was to assess knowledge, attitute and practice of local people toward influenza pandemic (H1N1) prevention after launching education programs since early pandemic period (June 2009).