Early Nutritional Conditions Research Articles

Premature Brain Aging in Baboons Resulting from Moderate Fetal Undernutrition.

Contrary to the known benefits from a moderate dietary reduction during adulthood on life span and health, maternal nutrient reduction during pregnancy is supposed to affect the developing brain, probably resulting in impaired brain structure and function throughout life. Decreased fetal nutrition delivery is widespread in both developing and developed countries, caused by poverty and natural disasters, but also due to maternal dieting, teenage pregnancy, pregnancy in women over 35 years of age, placental insufficiency, or multiples. Compromised development of fetal cerebral structures was already shown in our baboon model of moderate maternal nutrient reduction. The present study was designed to follow-up and evaluate the effects of moderate maternal nutrient reduction on individual brain aging in the baboon during young adulthood (4–7 years; human equivalent 14–24 years), applying a novel, non-invasive neuroimaging aging biomarker. The study reveals premature brain aging of +2.7 years (p < 0.01) in the female baboon exposed to fetal undernutrition. The effects of moderate maternal nutrient reduction on individual brain aging occurred in the absence of fetal growth restriction or marked maternal weight reduction at birth, which stresses the significance of early nutritional conditions in life-long developmental programming. This non-invasive MRI biomarker allows further longitudinal in vivo tracking of individual brain aging trajectories to assess the life-long effects of developmental and environmental influences in programming paradigms, aiding preventive and curative treatments on cerebral atrophy in experimental animal models and humans.

Interacting effects of early dietary conditions and reproductive effort on the oxidative costs of reproduction.

The hypothesis that oxidative damage accumulation can mediate the trade-off between reproduction and lifespan has recently been questioned. However, in captive conditions, studies reporting no evidence in support of this hypothesis have usually provided easy access to food which may have mitigated the cost of reproduction. Here, I test the hypothesis that greater investment in reproduction should lead to oxidative damage accumulation and telomere loss in domestic zebra finches Taeniopygia guttata. Moreover, since the change or fluctuation in diet composition between early and late postnatal period can impair the ability to produce antioxidant defences in zebra finches, I also tested if early nutritional conditions (constant vs fluctuating early diet) influenced the magnitude of any subsequent costs of reproduction (e.g., oxidative damage and/or telomere shortening). In comparison to pairs with reduced broods, the birds that had to feed enlarged broods showed a higher level of oxidative DNA damage (8-OHdG), but brood size had no effect on telomeres. Fluctuating early diet composition reduced the capacity to maintain the activity of endogenous antioxidants (GPx), particularly when reproductive costs were increased (enlarged brood). The decline in GPx in birds feeding enlarged broods was accompanied by a change in bill colouration. This suggests that birds with lower endogenous antioxidant defences might have strategically increased the mobilization of antioxidants previously stored in other tissues (i.e., bill and liver) and thus, preventing an excessive accumulation of damage during reproduction.

Malnutrition increases NO production and induces changes in inflammatory and oxidative status in the distal colon of lactating rats.

Epidemiological studies have indicated the lack of breast feeding as a risk factor associated with later development of inflammatory bowel disease. Nevertheless, the repercussion of little feeding during suckling on large intestine inflammatory response and anti-oxidant resources has not yet been completely understood. This study hypothesized that unfavorable lactation is able to induce oxidative stress and release of inflammatory mediators modifying the integrity of the colon epithelium in weanling rats. Wistar rats were reared under different early nutritional conditions according to litter size in two groups: N6 (6 pups/dam) and N15 (15 pups/dam) until the 25th postnatal day. The distal colon was removed and processed for biochemical, morphometric, and immunohistochemical analyzes. Lipoperoxidation, nitric oxide (NO), reduced (GSH) and oxidized (GSSG) glutathione, tumor necrosis factor-alpha (TNF-α), interleukins-1β, 4 and 10 (IL-1β; IL-4; IL-10) levels, and total superoxide dismutase (tSOD), and catalase (CAT) activities were assessed. Morphometric analysis was carried out using paraffin sections and wholemount myenteric plexus preparations. Increased lipoperoxidation, NO, TNF-α and IL-1b levels, reduced tSOD and increased CAT activities were found in the N15 compared to N6 group. No intergroup difference was detected for IL-10, while lower levels of IL-4, GSH and GSSG and lower neuronal size and density were induced by undernutrition. Reduced feeding during suckling changed the inflammatory response and oxidative status in the colon of weanling rats. These data suggest potential mechanisms by which malnutrition early in life may increase the vulnerability of the large intestine to insults.

Long-term consequences of early nutritional conditions on the behaviour and growth of fire salamanders

Early developmental conditions can have a strong influence on the life history. Fire salamanders represent an interesting system due to their biphasic life cycle consisting of an aquatic larval stage and a subsequent terrestrial stage. The environment experienced as larvae affects growth, age at metamorphosis and behaviour. In particular, diet restrictions can cause developmental stress. However, the impact of such developmental stress on the growth and behaviour in a long-lived amphibian are not yet well studied. We manipulated the early diet of sibling pairs of fire salamander larvae. One sibling was raised under food-restriction and the other was raised under conditions of abundant food. We report long-term effects on growth and exploratory behaviour; exploratory behaviour was tested in two sessions, first as young juveniles and later as sub-adults. We found that food-restricted conditions during early development affected exploratory behaviour. Early deficits in body mass and body size were compensated later in life, by approximately 18 months of age. When morphological differences were compensated, the initial differences in exploratory behaviour also disappeared. Thus, compensation not only led to an alignment of body parameters but was also accompanied by an adjustment in exploratory behaviour. No cost of compensation was detected, but future research will need to verify whether the potential costs of compensation are negligible in slow-growing salamanders or whether they are shifted to later life stages. Our study provides novel insights into the life history of fire salamanders and reveals that early larval conditions have effects on individuals long after metamorphosis.

Effects of resource variation during early life and adult social environment on contest outcomes in burying beetles: a context-dependent silver spoon strategy?

Good early nutritional conditions may confer a lasting fitness advantage over individuals suffering poor early conditions (a ‘silver spoon’ effect). Alternatively, if early conditions predict the likely adult environment, adaptive plastic responses might maximize individual performance when developmental and adult conditions match (environmental-matching effect). Here, we test for silver spoon and environmental-matching effects by manipulating the early nutritional environment of Nicrophorus vespilloides burying beetles. We manipulated nutrition during two specific early developmental windows: the larval environment and the post-eclosion environment. We then tested contest success in relation to variation in adult social environmental quality experienced (defined according to whether contest opponents were smaller (good environment) or larger (poor environment) than the focal individual). Variation in the larval environment influenced adult body size but not contest success per se for a given adult social environment experienced (an ‘indirect’ silver spoon effect). Variation in post-eclosion environment affected contest success dependent on the quality of the adult environment experienced (a context-dependent ‘direct’ silver spoon effect). By contrast, there was no evidence for environmental-matching. The results demonstrate the importance of social environmental context in determining how variation in nutrition in early life affects success as an adult.

Lifelong consequences of early nutritional conditions on learning performance in zebra finches (Taeniopygia guttata)

Long-term effects of early developmental conditions on physiological and behavioural traits are common in animals. Yet, such lifelong effects of early life conditions on learning skills received relatively less attention, even though they are expected to have strong fitness effects. To test the lifelong impact of the early environment on associative and reversal learning performance, we tested zebra finches (Taeniopygia guttata) in a reversal learning task about five years after they were raised either under low or high quality food treatments in their first month of life. The early nutritional treatment and its respective growth patterns significantly influenced learning performance: Zebra finches who received a high-quality nutrition early in life gained more weight during the treatment period but needed more trials to associate a cue with a reward. The early growth rate during the treatment phase was linked to how fast the birds detected the food at the onset of training in our learning task as well as to their associative learning performance. However, in the reversal learning step of the task testing for behavioural flexibility, no differences with respect to early nutritional treatments or related growth rates were apparent. We show that early life conditions directly affect the approach to our task and learning abilities over an entire lifetime, emphasizing how crucial the early environment is for understanding adult behaviour throughout life.

Poor Nutritional Conditions During the Early Larval Stage Reduce Risk-Taking Activities of Fire Salamander Larvae (Salamandra salamandra)

Environmental conditions experienced early in life have been shown to significantly affect growth trajectories at later stages in many vertebrate species. Amphibians typically have a biphasic life history, with an aquatic larval phase during early development and a subsequent terrestrial adult phase after completed metamorphosis. Thus, the early conditions have an especially strong impact on the future survival and fitness of amphibians. We studied whether early nutritional conditions affect the behavioural reaction of fire salamander larvae (Salamandra salamandra) before completion of metamorphosis. Fire salamander larvae reared under rich nutritional conditions were heavier and larger, displayed better body condition overall throughout the first three month of life and metamorphosed earlier compared with larvae raised under poor nutritional conditions. Specifically, we tested whether larvae reared under these different conditions differed with respect to their risk-taking behaviour and activity. We found no differences in the activity of larvae with respect to their experienced early food conditions. However, larvae reared under poor nutritional conditions hid significantly more often in a risk-taking test than larvae reared under rich food conditions. This increase in shelter-seeking behaviour might be an adaptation to reduce the risk of larval drift or an adaptation to compensate for physiological deficits in part by appropriate behavioural reactions. Our results indicate that environmental conditions, such as food availability, may lead to different behavioural strategies.

Early Fasting Is Long Lasting: Differences in Early Nutritional Conditions Reappear under Stressful Conditions in Adult Female Zebra Finches

Conditions experienced during early life can have profound effects on individual development and condition in adulthood. Differences in nutritional provisioning in birds during the first month of life can lead to differences in growth, reproductive success and survival. Yet, under natural conditions shorter periods of nutritional stress will be more prevalent. Individuals may respond differently, depending on the period of development during which nutritional stress was experienced. Such differences may surface specifically when poor environmental conditions challenge individuals again as adults. Here, we investigated long term consequences of differences in nutritional conditions experienced during different periods of early development by female zebra finches (Taeniopygia guttata) on measures of management and acquisition of body reserves. As nestlings or fledglings, subjects were raised under different nutritional conditions, a low or high quality diet. After subjects reached sexual maturity, we measured their sensitivity to periods of food restriction, their exploration and foraging behaviour as well as adult resting metabolic rate (RMR). During a short period of food restriction, subjects from the poor nutritional conditions had a higher body mass loss than those raised under qualitatively superior nutritional conditions. Moreover, subjects that were raised under poor nutritional conditions were faster to engage in exploratory and foraging behaviour. But RMR did not differ among treatments. These results reveal that early nutritional conditions affect adult exploratory behaviour, a representative personality trait, foraging and adult's physiological condition. As early nutritional conditions are reflected in adult phenotypic plasticity specifically when stressful situations reappear, the results suggest that costs for poor developmental conditions are paid when environmental conditions deteriorate.

Early nutritional conditions, growth trajectories and mate choice: does compensatory growth lead to a reduction in adult sexual attractiveness?

It is well documented that poor early nutrition can have profound negative effects on adult life-history traits. However, it has also been demonstrated that organisms can undergo compensatory resource allocation strategies (such as an accelerated growth rate) if food availability improves, so as to mitigate the effects of the poor early conditions. Previous research has indicated that elevated growth rates can incur costs in the longer term, such as an increased rate of senescence and shorter lifespan. We tested whether a phase of compensatory growth after a period of reduced food availability earlier in life affected the sexual attractiveness of adult male green swordtails Xiphophorus helleri, a species in which it has previously been documented that females prefer larger-bodied and longer-tailed males. The experiment compared the attractiveness of size-matched brothers that had experienced contrasting growth trajectories as juveniles; the experiments were initially conducted in the middle of a male’s sexually reproductive life and were then repeated towards the very end of life. At both ages, males that had undergone compensatory growth were equally as attractive as their brothers that had grown normally. These results suggest that the growth compensation benefits males through an increase in their attractiveness over that which they would have had if they had remained on their original growth trajectory. The lack of change in relative attractiveness with age indicates that the compensation does not cause greater deterioration in secondary sexual characters at older ages than in continuously well-fed males.

Long-Run Longevity Effects of a Nutritional Shock Early in Life: The Dutch Potato Famine of 1846-1847

Background: Nutrition in utero and infancy may causally affect health and mortality at old ages. Until now, very few studies have demonstrated long-run effects on survival of early life nutrition, mainly because of data limitations and confounding issues. Methods: This paper investigates whether exposure to nutritional shocks in early life negatively affects longevity at older ages, using unique individual data and exploiting the exogenous variation implied by natural experiments. In particular, early nutritional conditions are instrumented by exposure to the potato famine of unprecedented severity that the Dutch faced in 1846-47. The individual data are from the Historical Sample of the Netherlands and are augmented by food price data and macro-economic data. The sample used in the study covers lifetimes of 398 individuals exposed and 1,342 individuals not exposed to severe famine during gestation and/or till age three. We compare non-parametrically the total and residual lifetimes of treated and controls per gender. We also estimate survival models in which we control for other individual characteristics and additional (early life) determinants of mortality. Results: Men exposed to severe famine during pregnancy (at least four months) and directly after birth have a significant lower residual life expectancy at age 50 than others, but not at earlier ages. We could not demonstrate any long-run effects for men exposed at ages 0-2 and for women. Conclusion: To our knowledge, this is the first evidence suggesting long-run effects of early nutritional stresses on mortality at old ages for men.

Effects of changes in nutritional conditions on timing of puberty: clinical evidence from adopted children and experimental studies in the male rat.

Among 32 patients with idiopathic central precocious puberty seen during a 3-year period, 1/4 were adopted children from developing countries who showed early sexual maturation during the catch-up process following their arrival in Belgium. To study the possible mechanism accounting for such clinical observations, we used the male rat as a model, and evaluated the effect of variations in early nutritional conditions, by manipulating litter size, on hypothalamic and testicular maturation. We had shown previously that, in the male rat, onset of puberty was preceded, between 15 and 25 days of age, by a transiently increased activation of N-methyl-D-aspartate receptors involved in a facilitatory control of pulsatile secretion of gonadotropin-releasing hormone. We also showed that the proportion of elongated spermatids in testicular cell homogenates increased between 25 and 45 days of age. When compared to pups of a small litter (6/dam), those of a large litter (14/dam) showed a reduced growth rate (1.9 vs. 3.5 g/day) before weaning (21 days), whereas they grew at a similar rate (5.6 vs. 4.7 g/day) after weaning. At 35 days of age, the animals raised in the large litter showed evidence of delayed hypothalamic and testicular maturation when compared to animals from the small litter. Reduction of litter size at 17 days allowed food-restricted pups of a large litter to resume a normal growth rate before weaning.(ABSTRACT TRUNCATED AT 250 WORDS)