Cultivars and growing media are important components of a successful greenhouse tomato (Solanum lycopersicum) operation. Two studies were conducted simultaneously and independently in two 30 × 96-ft greenhouses in Spring 2006 and 2007 (January–July) to assist producers in selecting appropriate cultivars and reducing production cost. The first study was conducted to evaluate yield, fruit weight, fruit quality, and shelf life of ‘Geronimo’, ‘Quest’, and ‘Trust’ tomatoes planted in perlite and pruned to three or four fruit per cluster. The second study was conducted to determine the initial cost of perlite, pine bark, and rockwool growing media and their effect on yield of ‘Quest’ pruned to three or four fruit per cluster. ‘Geronimo’ produced the highest total marketable yield and ‘Trust’ produced the lowest. ‘Trust’ produced more cull yield and lower fruit weight than ‘Geronimo’ or ‘Quest’. Pruning clusters to three fruit increased total marketable yield and fruit weight, and reduced cull yield of all cultivars. Only ‘Geronimo’ produced higher early marketable yield at four fruit per cluster. All cultivars produced higher early marketable yield in 2007 than in 2006. Tested cultivars had similar fruit content of potassium and sodium and similar concentration of soluble solids. ‘Trust’ fruit had a higher pH than the other two cultivars. About 92% of ‘Quest’ tomatoes remained marketable after storage at 67 °F for 1 week. ‘Geronimo’ and ‘Trust’ had only 83% and 78% marketable fruit, respectively, under the same conditions. Initial costs to grow greenhouse tomatoes in perlite were higher than in rockwool, and were the lowest in pine bark. Plants grown in perlite produced higher total marketable yield than plants grown in either of the other media. They produced lower cull yield than plants grown in rockwool, but produced similar cull yield to plants grown in pine bark. Pruning clusters to three fruit increased total marketable yield and fruit weight in both studies. Pruning clusters to four fruit increased cull yield in both studies regardless of planting year.
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