Early Life Stages Research Articles

Impacts of river flow and thermal regimes on fish growth dynamics during early life history

Growth during early life stages is critical to fish population persistence. Few studies explore how river discharge and thermal regimes impact growth dynamics of young-of-the-year (YOY) fishes. We used otolith biochronologies and generalised additive mixed models to partition the effects of age, individual, river, temperature, discharge, and variance in discharge on daily growth rates of YOY fishes with an opportunistic life-history strategy: Galaxias vulgaris and Gobiomorphus breviceps. Growth of both species increased with temperature. Galaxias growth decreased with discharge, while Gobiomorphus growth increased with lowered discharge but only to a point (ca. 1/3 of median discharge), after which growth plateaued. Floods had a strong negative effect on YOY growth. Our study systems and species had different characteristics to those that formed the basis of the Riverscape Recruitment Synthesis Model (RRSM) and may explain why our results were only partially consistent with flow predictions for opportunistic species of the RRSM. Further, Galaxias growth was a negative function of high-frequency, daily variation in flow, and we present testable hypotheses to explain this result.

Dynamics of metal/metalloid bioaccumulation and sensitivity in post-larvae shrimp (Macrobrachium rosenbergii) exposed to settleable atmospheric particulate matter from an industrial source.

The metallurgy industry is a potent global source of particulate matter (PM) atmospheric emissions. A portion of this PM may settle in aquatic (SePM) carrying metal/metalloid particles and metallic nanoparticles. Surprisingly, this form of contamination has not received due attention from most environmental monitoring agencies. We analyzed the effect of exposure to SePM on shrimp post-larvae, a critical stage for the viability of shrimp populations and for the trophic chain. After acclimation, shrimp were exposed to contaminants using a randomized experimental design-a 4 × 4 factorial arrangement with 2 factors: exposure time (24, 48, 72, and 96 h) and SePM concentration (0.00, 0.01, 0.10, and 1.00 g L-1). The bioaccumulation of metals, contamination rates, mortality, and ROS-related biomarkers (lipid peroxidation - LPO; DNA strand breakage DNA SB and metallothionein content - MTs;) were evaluated. After contamination, the water contained 27 different metals/metalloids. Post-larvae accumulated metals, such as Cd, Pb, Al, As, Se, Sr, Zr, Ba, La, Ce, W, and Hg. However, the rise in SePM did not result in a proportional bioaccumulation rise, indicating that effective biological barriers may work for some metals. Although the different levels of SePM changed mortality dynamics, they resulted in a similar final lethality (60-80 %). SePM caused significant damage to lipids (increased LPO), genetic material (DNA SB), and increased Mts. Such effects may reflect a particularly deleterious ecological problem as it is present at such an early stage of life. These results identified a clear environmental risk since the lower level of exposure used was 102 times lower than that measured in the habitats affected by local industry. Consequently, our results emphasize the need for clear protocols for monitoring the effects of SePM in aquatic environments.

External morphology and growth patterns of larvae and juveniles of Bryconops gracilis (Characiformes, Iguanodectidae) from Amazon basin

During early development, fishes undergo significant changes that influence external morphology and the functioning of internal organs and systems. This often results in gradual variation of the morphological traits of individuals across developmental stages. The investigation of larval and juvenile fish development and growth patterns has pertinent implications for the systematic and ecological elucidation of species. Bryconops gracilis is a medium-sized fish, omnivorous that inhabits lotic and lentic environments with acidic and transparent waters in the Amazon basin. In this study, the early development of B. gracilis is described, until recently a practically unknown species. In terms of development, we used morphological, meristic, and morphometric data to characterize the larvae and juveniles. The individuals were collected in the Curuá-Una River, Amazon basin, Brazil. Fifty-four specimens were examined. Samples include individuals with 3.39 to 21.79mm SL. Yolk-sac larvae have two attachment organs on the dorsal surface of head and body. The larvae of B. gracilis are considered altricial, with a fusiform body, and the intestine reaches the median region of the body. Initially, the mouth is subterminal and becomes isognathic from the postflexion stage on. During the postflexion stage, the most relevant morphological changes occur (e.g., presence of all fins, mouth position similar to adults, increased body pigmentation), making individuals more specialized to explore new habitats and diets and maximize their chances of survival. Furthermore, vertebrae and myomeres are compared and assist with differentiating some Bryconops species at early life stages that occur in sympatry in the Amazon basin. Our results contribute to knowledge about the external morphology of neotropical freshwater fishes, enabling the identification of larvae and juveniles through traditional taxonomy and broadening the perspective on the ontogenetic study of the adipose fin in Characoidei.

Make it a standard? The creation and variability assessment of a consensus standard protocol for Tenebrio molitor larvae feeding trials

Abstract Interest in the nutrition of the yellow mealworm (Tenebrio molitor L.) larvae is on the rise, leading to an increase in publications on this topic. The absence of a standard protocol and resulting differences in experimental designs reduces comparability among studies and impedes research on mealworm nutrition. To address this, a consensus standardised protocol was developed specifically for the evaluation of mealworm larval growth and performance in feeding trials. The efficacy of this protocol was evaluated through an international ring test involving seven partners using two wheat brans as dry feed (a standard bran and a local bran) at 27 °C and 60% relative humidity. As experimental units, plastic crates filled with 2.1 kg of bran and 10,000 4-week-old larvae were used with six replicates. Agar gel was provided as wet feed ad libitum. The mean individual larval weight and the number of larvae per crate were determined weekly until either three or more replicates ran out of feed or pupation exceeded 10%. At harvest, the total larval fresh biomass and amount of frass was determined. Larval samples were taken for chemical analysis. To assess the protocol, the within (repeatability) and between (reproducibility) laboratory variability was calculated for each parameter. The repeatability was good (limit at 12% (standard) and 14% (local)). The reproducibility was poorer with a limit 2.7 times higher for the standard feed (36%) and 3.8 times higher for the local feed (55%). For both feeds, the total larval fresh harvest, amount of frass and the larval protein concentration were the most consistent both within and among laboratories. The highest variability was observed at the early life stages and for the larvae ash content. The detailed consensus standard protocol and repeatability/reproducibility estimates can be used as basis for future mealworm feeding trials, comparing results and future improvements.

Influence of Spawning and Nursery Ground Environmental Changes on Walleye Pollock Catches Along the Eastern Coasts of Korea and Japan After the Late-1980s Climate Regime Shift

The eastern coasts of Korea (ECK) and Japan (ECJ) are located at the southernmost limit of walleye pollock distribution in the Northwest Pacific. Following the climate regime shift (CRS) in the late 1980s, pollock catches in these regions have declined sharply, with different trends emerging. This study examined the relationship between environmental factors, such as sea surface temperature (SST) and habitat suitability, and changes in pollock catches from the late 1980s to 2022. From the late 1980s to the late 1990s, El Niño and positive Pacific decadal oscillation (PDO) phases dominated, increasing SST in the ECK and ECJ habitats and rapidly decreasing catches. Although spawning grounds (SGs) have maintained high habitat suitability, nursery ground (NG) suitability has declined. From the late 1990s to 2022, La Niña and negative PDO phases prevailed, with SST continuing to rise along the ECK, further reducing catches. SG suitability remained high, but NG suitability declined. Along the ECJ, SST decreased after the late-1990s CRS, stabilizing catches. After the mid-2010s, the SST increased along the ECJ, reducing pollock catches, although SG suitability remained high. This study elucidates SST changes during early life stages and their effects on pollock catch, habitat, and resources in future marine environments.

X‐Ray Absorption and X‐Ray Emission Spectroscopy Study of a Molybdenum Disulfide Anode in a Sodium‐Ion Half‐Cell

The development of efficient anodes for sodium‐ion batteries requires a deep understanding of the electrochemical processes involved. Herein, X‐ray spectroscopy is used to study changes in the electronic structure of MoS2 nanosheets coupled with few‐layered graphene at early stages of battery life. Working electrode is combined with a sodium plate and a solution of NaClO4 in ethyl carbonate/dimethyl carbonate in a homemade cell having an X‐ray transparent window. X‐ray absorption spectra at the S K‐edge and Mo L3‐edge are recorded using a synchrotron source, X‐ray emission S Kα and Mo Lα spectra are obtained on a laboratory spectrometer for initial powder and electrode material at different potentials of the cell. Analysis of the spectra shows the occupation of the initially empty orbitals of MoS2 by sodium electrons as a result of the interaction of the components of the sodium‐ion half‐cell. Ex situ X‐ray photoelectron spectroscopy measurements of a fully discharged electrode material reveal partial transformation of hexagonal MoS2 to disordered tetragonal MoS2 coated with a surface electrolyte interphase layer. The obtained results indicate that coupling MoS2 to graphene prevents the breaking of Mo–S bonds when Na+ ions are first introduced, which may promote stable battery performance.

Association between bisphenol A exposure and cardiometabolic outcomes: a longitudinal approach

Abstract Background Increased cardiometabolic risk is associated with abnormalities in blood biomarkers profile and adiposity measurements. Some substances found in the food matrix or the environment, called endocrine-disrupting chemicals, may impair cardiometabolic health in the yearly and later stages of life. Bisphenol A (BPA) is a food contaminant that migrates from food contact materials and may act as an endocrine disruptor, negatively affecting human health. The present work aims to assess the longitudinal association between BPA exposure and cardiometabolic outcomes. Methods Data from the four waves of the Portuguese population-based birth cohort Generation XXI were used (n = 3138). Dietary data were collected by 3-d food diaries, using the FoodEx2 classification system to describe the reported foods. BPA daily exposure was estimated using a random forest model, combining dietary information with urinary BPA measured in 24-h urine from a subsample, used to predict the remaining sample’s BPA exposure. The generalised least squares model was used to test the associations between BPA and cardiometabolic outcomes. Results Blood insulin and HOMA-IR presented a significant longitudinal association with BPA daily exposure after adjustment for important variables and energy (0.06 SD(0.03, 0.09); 0.05 SD(0.02, 0.08) respectively). The same findings were observed for fat mass and waist circumference (0.03 SD(0.01, 0.06); 0.06 SD(0.04, 0.08), respectively). For z-BMI, a significant cross-sectional and longitudinal association was found (0.03 SD(0.01, 0.04); 0.02 SD(0.00, 0.04), respectively). Conclusions This was the first study assessing the association between BPA exposure and health outcomes from childhood to adolescence. We found an association between BPA exposure and an increased blood insulin level, insulin resistance, fat mass percentage, waist circumference and z-score of body mass index. Our results point to the need to reduce exposure to BPA in the early stages of life. Key messages • BPA exposure was estimated using direct and indirect methods. • BPA can impair cardiometabolic outcomes from the early stages of life, such as higher blood insulin level, insulin resistance, fat mass, waist circumference, and body mass index.

Nutrition of children up to one year of age what public health topic

Abstract Background Nutrition is one of the most important factors for a person that affects the quality and length of life. Nutrition has a fundamental influence on the maintenance of health or the development of disease and participates in the correct growth and development of an individual. Nutrition is especially important in the early stages of life, when a child’s organism develops dramatically. Mothers have the greatest influence on a child’s nutrition, and therefore it is advisable to educate them appropriately and also educate them early, from child birth or before. And early nutrition is provided through breastfeeding. Objectives To improve the knowledge of healthcare workers in the field of breastfeeding and nutrition of children up to one year of age. To strengthen the breastfeeding skills of mothers. To motivate healthcare workers to educate mothers in maternity wards and GP offices. Results Using a questionnaire, we analyzed the knowledge of 716 mothers of children under two years of age. 91 % of female respondents knew that the child should be breastfed according to his/her needs. Only 66 % of women were educated and informed about the importance and technique of breastfeeding. Educational material was prepared and distributed for midwives, nurses and doctors on breastfeeding and feeding children up to one year of age. Conclusions The level of awareness of health professionals about breastfeeding and nutrition is still insufficient. There is a need to continue to educate and support healthcare workers in this area and to motivate them in providing information to mothers. Key messages • Breastfeeding and feeding children up to one year of age are important for a child’s health. • The advisory activity of healthcare workers is key to improving public health. Health workers should help mothers not only with advice but also with practical help with breastfeeding.

Overcoming barriers in implementing healthy school nutrition policies: insights from Ukraine

Abstract The general mortality rate from NCDs in Ukraine exceeds that in European Union countries, (Yurochko et al., 2023) exacerbated by the ongoing war. Healthy nutrition plays a pivotal role in enhancing the overall quality of life and curbing the prevalence of non-communicable diseases (NCDs). Despite its significance, ensuring healthy eating habits, particularly during the early stages of life at the period of setting lifelong habits (Jackson et al., 2020; Riley et al., 2018), remains a challenge. Besides, school-based settings represent an ideal environment for effective intervention because they offer a shared common physical, academic, and social space (Tapia-Fonllem et al., 2020). Through a series of semi-structured interviews with key stakeholders, this study identifies barriers and facilitators to policy implementation, with a focus on educational institutions. Qualitative analysis reveals barriers such as inadequate human resources, funding constraints, resistance from the food industry, and ineffective communication strategies at the family level. Moreover, there exists a disjointed understanding of nutrition standards. However, collaborative efforts with civil society, empowerment of nursing roles, strategic utilization of grants and fundraising, engagement with the food industry, and the adoption of a family-oriented approach emerge as crucial enablers for successful policy implementation. These findings offer theoretical foundations for fostering healthier nutrition environments within schools. This research serves as a roadmap for policymakers and educators striving to create sustainable and effective strategies for promoting healthy eating among early school-age children in Ukraine and beyond. Study finds human resource shortages, funding constraints, and food industry resistance as major barriers to school nutrition policy. Recommendations include collaborative approaches, nurse involvement, and aligned standards for effective school nutrition policy. Key messages • Healthy nutrition plays a pivotal role in enhancing the overall quality of life and curbing the prevalence of non-communicable diseases. • Through a series of semi-structured interviews with key stakeholders, this study identifies barriers and facilitators to policy implementation, with a focus on educational institutions.

Simultaneous degradation of cyanobacteria and cyanotoxins by catalytic wet peroxide oxidation: Impact of morphology and growth stage

The widespread proliferation of potentially toxic cyanobacteria in source waters for drinking water treatment plants (DWTPs) presents a pressing contemporary challenge. Although catalytic wet peroxide oxidation (CWPO) has been recognized as a highly effective and environmentally friendly technology for removing cyanotoxins from water, the simultaneous degradation of cyanobacteria and cyanotoxins has been scarcely investigated. Considering their great morphological diversity, in this work, the impact of the presence of pure strains of several species of toxic and non-toxic cyanobacteria on the degradation of relevant cyanotoxins has been evaluated considering also the main stages of their life cycle. In each studied scenario, achieving a full eradication of the cyanobacteria also led to the complete elimination of the cyanotoxin, including both intra and extracellular fractions. Morphological factors, including filament and cell sizes, play a crucial role in the efficiency of the process. Small cells and long filaments entail a higher presence of particulate organic matter, which consumes hydroxyl radicals considerably in competence with the cyanotoxin degradation. Moreover, the growth stage emerges as a pivotal factor in understanding the CWPO process performance, with cyanobacteria in their early life stages, exhibiting more pronounced interference compared to later stages, probably due to their higher cell viability and accelerated metabolism. All these understandings are essential for the successful implementation of the technology in treating surface waters affected by toxic cyanobacterial blooms.

Defining clavicle growth in infancy using chest radiographs

BackgroundDespite the critical role of the clavicle in understanding growth and development in early childhood, there remains a notable paucity of comprehensive studies investigating clavicle growth patterns during this crucial period. This hinders our ability to establish normative growth parameters during these early life stages. Our study sought to measure clavicle dimensions and subsequently construct growth curves spanning from preterm infants to toddlers up to the age of 6 years by measuring routine chest radiographs. Differences between both sides of the body and between the sexes should be analysed. This aimed to provide a nuanced understanding of clavicle growth dynamics and offering a foundation for the establishment of normative values in this understudied context. In this retrospective study, children aged 23 weeks of gestation to 6 years who underwent a chest radiography between January 2010 and June 2020 were enrolled. A total of 5.311 potential radiographs was screened. Clavicle length and width were measured in all radiographs using the Centricity™ Universal Viewer. Statistical analysis was performed using SPSS®.Results1.340 images met the quality criteria to be included in our study. The growth curves of clavicle lengths and widths showed a steadily increasing trend with age. Inclusion of premature infants in the age group of one month resulted in a decrease in this age group. Significant differences between both sides of the body and between the sexes could be shown. Measurements of clavicle length and width, derived from routine chest radiographs, are highly accurate. This accuracy underscores the potential for utilizing thoracic radiographs as a reliable tool for assessing clavicle growth in clinical settings or even forensic analysts. The establishment of reference values derived from our measurements provides a basis for normative growth parameters.

Class and Ethno-Gender Differences in Education and Labour Market Position—An Intersectional Analysis of Ethnic Integration in the UK

This paper analyses the socio-economic disadvantages of women from different ethnic minority heritages in the UK. Using data from the Labour Force Survey (2014–2023), which contains detailed information on parental class and respondents’ socio-economic conditions, we examine four domains of life chances which are crucial for ethnic integration: educational attainment at the degree level, risks of unemployment, access to professional-managerial (salariat) position and earning power. We proceeded with the gross differences and then examined the differences by ethno-gender status and parental class combinations, controlling for many confounding factors. We also examined the net ethno-gender differences over the life course and the trends of social fluidity over the period covered and across the ethno-gender groups. We found that women from all ethnic origins were doing well in education but faced multiple disadvantages in the labour market, especially in access to the salariat and in earning power. Women of Pakistani/Bangladeshi heritages faced pronounced unemployment risks, especially at the earlier life stages. There is a significant increase in fluidity over the period covered, but this is marked by considerable ethnic and class differences, with Black Caribbean, Black African, Pakistani and Bangladeshi women from more advantaged class origins being unable to secure advantaged class positions and those from working-class families unable to make long-range upward mobility as effectively as White men. Overall, Bangladeshi, Pakistani, Black African and Black Caribbean women are found to be considerably disadvantaged, but there are also signs of social progress.

ANKS6 Variants Underlie Polycystic Kidneys in Prenatal and Neonatal Cases

Background: Nephronophthisis (NPHP) is an autosomal recessive genetic disorder that can cause early-onset kidney failure. ANKS6 plays an important role in early kidney development and encodes a protein that interacts with other proteins within the primary cilium. ANKS6 mutations are known to cause nephronophthisis 16 (NPHP-16). Little is known regarding fetal ultrasound imaging and the antenatal diagnosis of fetuses with ANKS6-associated kidney disease. Here, we report the detection of ANKS6 variants in consanguineous families with polycystic kidney antenatally and in the early stages of life. Methods: Three unrelated Saudi Arabian patients (two prenatal patients and one neonate) were investigated. These cases were referred to the hospital due to the presence of echogenic kidneys on antenatal scanning. After clinical and phenotypic evaluation, whole-exome sequencing (WES) was performed on the cord and peripheral blood to identify the molecular genetic causes associated with the echogenic kidney phenotypes. Results: Two homozygous sequence variants were detected in ANKS6. The homozygous missense novel variant ANKS6: c.1159A>C was detected in Families 1 and 2. In the third family, the known homozygous loss-of-function variant ANKS6: c.907+2T>A was detected. Conclusions: We identified homozygous ANKS6 variants in three families presenting with antenatal polycystic kidney disease. The findings provide an expanded clinical presentation of ANKS6 and emphasize the utility of WES in the diagnosis of echogenic kidneys in prenatal settings.

Toxicity of crude oil: a systematic review of the acute impacts on Cnidaria

Cnidarians are ecosystem engineers that play a fundamental role in food webs. However, they are increasingly threatened by anthropogenic activities, such as oil spills, which can cause acute sublethal effects and in some cases, mortality. The literature on this theme is limited and fragmented, with diverse objectives and methodologies, making it challenging to identify consistent patterns and knowledge gaps. This article aims to provide a systematic review of the acute impacts of crude oil and petroleum hydrocarbons on members of the Phylum Cnidaria, to synthesize and integrate the available data. The research followed the PRISMA 2020 guidelines, and employed the search terms: “crude oil” AND “acute” AND (Cnidaria OR coral), along with the names of each Cnidarian class. The search was restricted to articles published in English from 2013 to 2023, excluding studies that did not directly address the core topic. The number of records collected was relatively low, with only 30 articles identified from 20 studies. The highest number of publications occurred in 2022, and the USA was the most represented country. The Class Anthozoa, particularly corals (Order Scleractinia) was the most studied. Research focused on the effects of crude oil on gametes, embryos, larvae and adults, with most studies examining adult organisms. The majority (56.6%), utilized mixtures of crude oil hydrocarbons, while a smaller proportion (36.6%) focused on pure aromatic hydrocarbons. Most experiments employed Water Accommodated Fractions (36.6%) and continuous flow system (20%). The most common exposure durations (30%) were 96 and 48h, with exposure times ranging from 18h to several days. There was significant variation in methodological approaches, underscoring the need for standardized protocols. Research on adult cnidarians primarily assessed health and morphology, lethality, genetic alterations, and symbiont efficiency. In contrast, studies on early life stages focused on lethality, larval metamorphosis and settlement. Overall, the results indicate that responses vary considerably, particularly concerning exposure time and oil concentration, with sublethal effects at different levels. Further studies incorporating a broader range of species, novel approaches, and the combined effects of elevated temperature and UV radiation are crucial to advancing our understanding of oil impacts on cnidarians.

Fostering a “Culture of Conflict” among Children

This article aims to explore the constructive resolution of interpersonal conflicts, with a particular focus on childhood. It highlights the necessity for “conflict education” and the cultivation of a “culture of conflict” from early stages of life, emphasizing the importance of developing appropriate competencies during childhood for harmoniously resolving both personal and interpersonal conflicts. These are especially emotional-social and cognitive skills as well as problem-solving abilities. Well-designed programs for fostering constructive conflict resolution among children, along with specific methods and heuristic techniques, facilitate the development of a range of these competencies in children.

Assessing climatic conditions and biotic interactions shaping the success of Cystoseira foeniculacea early-life stages.

Early-life stages of canopy-forming macroalgae are critical for the maintenance of natural populations and the success of restoration actions. Unfortunately, the abiotic conditions and biotic interactions shaping the success of these stages have received less attention than the interactions shaping the success of adults. Here, we combined field and mesocosm experiments to explore the effects of temperature, herbivory, and canopy presence on the development of early-life stages of the brown seaweed Cystoseira foeniculacea. We assessed these effects by examining changes in recruit density and size. After recruiting zygotes under laboratory conditions, we conducted one laboratory and three field experiments. In the first field experiment, the density of recruits decreased over time in all rockpools and was negatively affected by rising temperatures and turf cover. Additionally, a marine heatwave (MHW; 11 days >25°C) was recorded in the donor pools, producing strong decay in the density of transplanted recruits and a significant reduction of the mature canopy. The second field experiment tested the survival of recruits based on their positioning within the canopy. We observed a higher density of recruits when placed at the edge or outside the canopy compared to recruits placed under the canopy. In the third field experiment, an herbivory-exclusion experiment, we show how density of recruits decreased in less than 48 h in noncaged treatments. In the laboratory, we conducted a thermotolerance experiment under controlled conditions, exposing the recruits to 19, 22, 25, 28, and 31°C for 7 weeks to assess thermal impacts on their survival and growth. Temperatures above the 25°C threshold reduced the density and size of the recruits. This study sheds light on the performance of the early-life stages of a Cystoseira spp. in Macaronesia, showing a low survival ratio against the current pressures even in the context of the potential refuge provided by the intertidal rockpools.

Thraustochytrids and Algae as Sustainable Sources of Long-Chain Omega-3 Fatty Acids for Aquafeeds

There is limited ability to biosynthesize long-chain omega-3 fatty acids such as EPA and DHA in food webs leading to humans. Seafood is the key source of ω3 LC-PUFA, with aquaculture expected to meet rising global demand; however, marine fish have a high dietary requirement for EPA and DHA themselves. This was traditionally met using unsustainable dietary fish oil and fish meal, but limited supply and environmental concerns have dictated research on replacements. Among the industrial sources of EPA and DHA, microalgae and especially thraustochytrids stand out as resources with high concentrations. Although unicellular, thraustochytrids are not microalgae as they are not photosynthetic but instead are microheterotrophs. This removes the light requirement and facilitates high yields of monoculture for the production of single-cell oils. The availability, in high concentrations, of usually one or the other essential fatty acid permits a calibration of the EPA and DHA dose, which is especially useful as their effects have mainly been considered together in medical and aquaculture research. EPA and DHA have different effects on cell function and are precursors of different bioactive compounds. Using thraustochytrids, microalgae, and heterotrophic dinoflagellates, the importance of DHA has been investigated. DHA was essential for optimizing the growth of the early life stages of scallops and finfish and was preferentially incorporated into fish membrane phospholipids. The production of microalgae and microheterotrophs can contribute to the treatment of wastewater and waste gas, further enhancing their sustainability and reducing the environmental costs of aquaculture.

A transcriptomics-based analysis of mechanisms involved in the neurobehavioral effects of 6PPD-quinone on early life stages of zebrafish

As an emerging pollutant frequently detected in aquatic ecosystems, the toxicity of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine-quinone (6PPD-quinone) on fish has been confirmed, but insight into the mechanisms underlying those adverse effects is still limited. Thus, we exposed zebrafish embryos to 6PPD-quinone at 0, 0.25, 2.5, and 25 μg/L until 120 h post-fertilization (hpf), and investigated the variations in their development, behavior, monoamine neurotransmitter levels, and transcriptional profile. Exposure to 6PPD-quinone notably elevated the heart rate of zebrafish at 48 hpf (at 2.5 and 25 μg/L) and 72 hpf (at 0.25, 2.5, and 25 μg/L). In the dark-light transition test, the locomotor activity of zebrafish larvae exposed to 6PPD-quinone significantly increased, especially in the dark periods. Exposure to 6PPD-quinone also altered the dopamine level and its turnover in zebrafish, which exhibited significant correlations to their locomotor activity. RNA sequencing identified 394 differentially expressed genes (DEGs), most of which have the molecular function of binding and catalytic activity. Five DEGs were predicted as the key driver genes in the protein-protein interaction networks associated with circadian rhythm (i.e., npas2), protein processing in endoplasmic reticulum (i.e., hsp90b1 and pdia4), and estrogen signaling pathway (i.e., hsp90aa1.1 and hsp90aa1.2). Our findings provide more insights into mechanisms underlying the toxicity of 6PPD-quinone to teleosts and highlight the necessity to assess its potential risks to aquatic ecosystems.

Providing Insight for Pediatric Ear Surgery: Analysis of Middle Ear Development via HRCT.

Providing insight for pediatric ear surgery via investigations on the development patterns of ossicles, mastoid, and external auditory canal (EAC). This retrospective study analyzed high-resolution computed tomography (HRCT) scans of 191 healthy temporal bones ranging from infants to adults. Subjects were grouped by 1-year intervals for developmental regression models and 3-year intervals for stage comparisons using t-tests or Mann-Whitney U tests. The size of auditory ossicles and tympanic cavity (TC) remained stable during development, while the minimum diameter of the tympanic sinus (TS) entrance was reduced. Regarding mastoid pneumatization, the air cells can be observed at birth, became pronounced at 2 years old, and were fully developed around the age of 5, with subsequent growth primarily involving radial expansion. Furthermore, the EAC demonstrated significant growth with age: the width of EAC increased linearly ( = 0.12x + 4.01, R2 = 0.85), while the length of EAC followed a polynomial growth pattern ( = -0.03x2 + 1.15x + 6.25, R2 = 0.96). Ossicles and TC remain stable during development. Furthermore, mastoid air cells may have developed in the early stages of life, while their diameter increases synchronously with EAC. All in all, ossicular chain reconstruction surgery and endoscopic ear surgery can be performed in babies. NA Laryngoscope, 2024.

Epilepsy in Angelman syndrome and the most common electroencephalographic findings

Angelman syndrome is a genetic disorder characterised by severe mental retardation, subtle dysmorphic facial features, a characteristic behavioural phenotype, seizures and abnormalities in video electroencephalograms (video EEG). Angelman syndrome may be associated with genetic mechanisms involving the region of chromosome 15q11-13. Up to 90% of cases have epileptic seizures, usually in the early years of life. Videoelectroencephalography patterns with some typical characteristics associated with Angelman syndrome have been reported, although these are not specific to it, and as such it is also useful for early diagnosis, especially in the first months or years of life. To characterise the videoelectroencephalography findings of 17 patients diagnosed with Angelman syndrome, and compare them with previously published studies. We conducted a retrospective observational study of 34 video EEGs performed on 17 patients diagnosed with Angelman syndrome at the clinical neurophysiology service of the Puerta de Hierro University Hospital in Madrid between 2019 and 2022. The primary objective was to characterise the videoelectroencephalographic findings and compare them with previously published studies. As secondary objectives, we analysed the patterns proposed by Dan and Boyd, and other demographic, genetic and clinical data. Video EEG supported the clinical suspicion in our study, as baseline brain activity was altered in all the patients. We identified a pattern similar to those defined by Dan and Boyd in 88% of the cases, and the type III pattern was the most common in our series. These findings confirm that video EEG is highly sensitive for the diagnosis of Angelman syndrome, and very useful as a diagnostic biomarker in the early stages of life.