Early Inflorescence Development Research Articles

Exploration and Validation of the Potential Downstream Genes Underlying ipa1-2D Locus for Rice Panicle Branching

In recent years, some super hybrid rice varieties were bred with strong culms and large panicles, which are mainly contributed by the <i>ipa1-2D</i> locus. A gain-of-function allele of <i>OsSPL14</i> is the <i>ipa1-2D</i> and it can greatly increase the panicle primary branch number. However, the key downstream genes mediating this trait variation are not fully explored. In this study, we developed high-quality near-isogenic lines (NILs) with a difference of only 30 kb chromosomal segment covering the <i>ipa1-2D</i> locus. Using the NILs, we explored the impact of <i>ipa1-2D</i> on five sequential stages of early inflorescence development, and found that the locus can greatly enhance the initiation of primary branch meristems. A transcriptomic analysis was performed to unveil the downstream molecular network of <i>ipa1-2D</i>, and 87 genes were found differentially expressed, many of which are involved in metabolism and catalysis processes. In addition, transgenic lines of overexpression and RNA interference were generated to shape different levels of <i>OsSPL14</i>. They were also used to validate the expression variation explored by transcriptome. Based on the gene annotation, twelve potential downstream targets of <i>ipa1-2D</i> were selected, and their expression variation was confirmed by qRT-PCR analysis both in NILs and transgenic lines. This research expands the molecular network underlying <i>ipa1-2D</i> and provides novel gene information which might be involved in the control of panicle branching. We discussed the potential function of identified genes and highlighted their values for future function exploration and breeding application.

Stepwise increases in FT1 expression regulate seasonal progression of flowering in wheat (Triticum aestivum).

Flowering is regulated by genes that respond to changing daylengths and temperature, which have been well studied using controlled conditions; however, the molecular processes underpinning flowering in nature remain poorly understood. Here, we investigate the genetic pathways that coordinate flowering and inflorescence development of wheat (Triticum aestivum) as daylengths extend naturally in the field, using lines that contain variant alleles for the key photoperiod gene, Photoperiod-1 (Ppd-1). We found flowering involves a stepwise increase in the expression of FLOWERING LOCUS T1 (FT1), which initiates under day-neutral conditions of early spring. The incremental rise in FT1 expression is overridden in plants that contain a photoperiod-insensitive allele of Ppd-1, which hastens the completion of spikelet development and accelerates flowering time. The accelerated inflorescence development of photoperiod-insensitive lines is promoted by advanced seasonal expression of floral meristem identity genes. The completion of spikelet formation is promoted by FLOWERING LOCUS T2, which regulates spikelet number and is activated by Ppd-1. In wheat, flowering under natural photoperiods is regulated by stepwise increases in the expression of FT1, which responds dynamically to extending daylengths to promote early inflorescence development. This research provides a strong foundation to improve yield potential by fine-tuning the photoperiod-dependent control of inflorescence development.

Inflorescence Development and Floral Organogenesis in Taraxacum kok-saghyz.

Rubber dandelion (Taraxacum kok-saghyz Rodin; TK) has received attention for its natural rubber content as a strategic biomaterial, and a promising, sustainable, and renewable alternative to synthetic rubber from fossil carbon sources. Extensive research on the domestication and rubber content of TK has demonstrated TK’s potential in industrial applications as a relevant natural rubber and latex-producing alternative crop. However, many aspects of its biology have been neglected in published studies. For example, floral development is still poorly characterized. TK inflorescences were studied by scanning electron microscopy. Nine stages of early inflorescence development are proposed, and floral micromorphology is detailed. Individual flower primordia development starts at the periphery and proceeds centripetally in the newly-formed inflorescence meristem. Floral organogenesis begins in the outermost flowers of the capitulum, with corolla ring and androecium formation. Following, pappus primordium—forming a ring around the base of the corolla tube—and gynoecium are observed. The transition from vegetative to inflorescence meristem was observed 21 days after germination. This description of inflorescence and flower development in TK sheds light on the complex process of flowering, pollination, and reproduction. This study will be useful for genetics, breeding, systematics, and development of agronomical practices for this new rubber-producing crop.

Feeling the heat: developmental and molecular responses of wheat and barley to high ambient temperatures.

The increasing demand for global food security in the face of a warming climate is leading researchers to investigate the physiological and molecular responses of cereals to rising ambient temperatures. Wheat and barley are temperate cereals whose yields are adversely affected by high ambient temperatures, with each 1 °C increase above optimum temperatures reducing productivity by 5–6%. Reproductive development is vulnerable to high-temperature stress, which reduces yields by decreasing grain number and/or size and weight. In recent years, analysis of early inflorescence development and genetic pathways that control the vegetative to floral transition have elucidated molecular processes that respond to rising temperatures, including those involved in the vernalization- and photoperiod-dependent control of flowering. In comparison, our understanding of genes that underpin thermal responses during later developmental stages remains poor, thus highlighting a key area for future research. This review outlines the responses of developmental genes to warmer conditions and summarizes our knowledge of the reproductive traits of wheat and barley influenced by high temperatures. We explore ways in which recent advances in wheat and barley research capabilities could help identify genes that underpin responses to rising temperatures, and how improved knowledge of the genetic regulation of reproduction and plant architecture could be used to develop thermally resilient cultivars.

The anti-ethylene growth regulator silver thiosulfate (STS) increases flower production and longevity in cassava (Manihot esculenta Crantz)

Cassava, which produces edible starchy roots, is an important staple food for hundreds of millions of people in the tropics. Breeding of cassava is hampered by its poor flower production, flower abortion, and lack of reproductive prolificacy. The current work determined that ethylene signalling affects floral development in cassava and that the anti-ethylene plant growth regulator silver thiosulfate (STS) mitigates the effects of ethylene on flower development. STS did not affect the timing of flower initiation, but improved early inflorescence and flower development as well as flower longevity such that flower numbers were increased. STS did not affect shoot and storage root growth. Studies of silver accumulation and treatment localization support the hypothesis that the beneficial effects of STS are confined to tissues of the shoot apex. The most effective timing of application was before inflorescence appearance extending to post-flower appearance. Based on this work a recommended protocol for STS use was developed. This work has the potential to improve methods for enhancing cassava flower development in breeding nurseries and thereby synchronize flowering of desired parents and enable the production of abundant progeny of desired crosses.

Genome-wide transcript analysis of inflorescence development in wheat.

The process of inflorescence development is directly related to yield components that determine the final grain yield in most cereal crops. Here, microarray analysis was conducted for four different developmental stages of inflorescence to identify genes expressed specifically during inflorescence development. To select inflorescence-specific expressed genes, we conducted meta-analysis using 1245 Affymetrix GeneChip array sets obtained from various development stages, organs, and tissues of members of Poaceae. The early stage of inflorescence development was accompanied by a significant upregulation of a large number of cell differentiation genes, such as those associated with the cell cycle, cell division, DNA repair, and DNA synthesis. Moreover, key regulatory genes, including the MADS-box gene, KNOTTED-1-like homeobox genes, GROWTH-REGULATING FACTOR 1 gene, and the histone methyltransferase gene, were highly expressed in the early inflorescence development stage. In contrast, fewer genes were expressed in the later stage of inflorescence development, and played roles in hormone biosynthesis and meiosis-associated genes. Our work provides novel information regarding the gene regulatory network of cell division, key genes involved in the differentiation of inflorescence in wheat, and regulation mechanism of inflorescence development that are crucial stages for determining final grain number per spike and the yield potential of wheat.

BELL1-like homeobox genes regulate inflorescence architecture and meristem maintenance in rice.

Inflorescence architecture is diverse in angiosperms, and is mainly determined by the arrangement of the branches and flowers, known as phyllotaxy. In rice (Oryza sativa), the main inflorescence axis, called the rachis, generates primary branches in a spiral phyllotaxy, and flowers (spikelets) are formed on these branches. Here, we have studied a classical mutant, named verticillate rachis (ri), which produces branches in a partially whorled phyllotaxy. Gene isolation revealed that RI encodes a BELL1-type homeodomain transcription factor, similar to Arabidopsis PENNYWISE/BELLRINGER/REPLUMLESS, and is expressed in the specific regions within the inflorescence and branch meristems where their descendant meristems would soon initiate. Genetic combination of an ri homozygote and a mutant allele of RI-LIKE1 (RIL1) (designated ri ril1/+ plant), a close paralog of RI, enhanced the ri inflorescence phenotype, including the abnormalities in branch phyllotaxy and rachis internode patterning. During early inflorescence development, the timing and arrangement of primary branch meristem (pBM) initiation were disturbed in both ri and ri ril1/+ plants. These findings suggest that RI and RIL1 were involved in regulating the phyllotactic pattern of the pBMs to form normal inflorescences. In addition, both RI and RIL1 seem to be involved in meristem maintenance, because the ri ril1 double-mutant failed to establish or maintain the shoot apical meristem during embryogenesis.

A Dynamic Co-expression Map of Early Inflorescence Development in Setaria viridis Provides a Resource for Gene Discovery and Comparative Genomics.

The morphological and functional diversity of plant form is governed by dynamic gene regulatory networks. In cereal crops, grain and/or pollen-bearing inflorescences exhibit vast architectural diversity and developmental complexity, yet the underlying genetic framework is only partly known. Setaria viridis is a small, rapidly growing grass species in the subfamily Panicoideae, a group that includes economically important cereal crops such as maize and sorghum. The S. viridis inflorescence displays complex branching patterns, but its early development is similar to that of other panicoid grasses, and thus is an ideal model for studying inflorescence architecture. Here we report a detailed transcriptional resource that captures dynamic transitions across six sequential stages of S. viridis inflorescence development, from reproductive onset to floral organ differentiation. Co-expression analyses identified stage-specific signatures of development, which include homologs of previously known developmental genes from maize and rice, suites of transcription factors and gene family members, and genes of unknown function. This spatiotemporal co-expression map and associated analyses provide a foundation for gene discovery in S. viridis inflorescence development, and a comparative model for exploring related architectural features in agronomically important cereals.

When is the best time to apply postharvest nitrogen fertiliser?

The effect of application timing of nitrogen to mango trees was investigated over 3 years to determine whether pre-harvest applications of N affected fruit quality, canopy growth, flowering and tree yield in 8-year-old 'Kensington Pride' (KP) and 'R2E2' mango trees growing in Far North Queensland. The experiment consisted of six treatments, where a total of 156 g N tree-1 was applied as 340 g urea at different application times and proportions. The treatments were applied as: 1) 100% postharvest at 2 weeks postharvest (control), 2) 50% 2 weeks pre-harvest plus 50% 2 weeks postharvest, 3) 35% 2 weeks pre-harvest plus 65% 2 weeks postharvest, 4) 65% 2 weeks pre-harvest plus 35% 2 weeks postharvest, 5) 35% 4 weeks pre-harvest plus 65% 2 weeks postharvest, and 6) 65% 4 weeks pre-harvest plus 35% 2 weeks postharvest. The results indicated that the pre-harvest applications of N did not significantly affect tree or orchard yield, fruit weight, size or number, nor did they negatively influence background skin colour or disease incidence at eating ripe in either cultivar, when compared with the control (100% N added postharvest). In the 'R2E2' trees, treatments 4 and 6 had an increased total panicle count. For the KP trees, pre-harvest applications of N in treatments 5, 6, 4 and 2 had increased stages of floral development when compared with the control trees, at the early stages of flowering. These results indicate that applications of N fertiliser prior to harvest can positively influence seasonal vegetative growth and early inflorescence development, but responses are cultivar specific. Therefore, N fertiliser applications can be added prior to harvest to encourage rapid floral and vegetative development after pruning without negative effects on fruit quality, particularly if N is applied as split applications at the recommended rate for tree size and cultivar.

Study on early inflorescence development in bread wheat (T. aestivum L.) lines with non-standard SCR-morphotype

Features of wheat (Triticum aestivum L.) inflorescence development define its architecture and have an impact on yield potential. Wheat lines and forms with altered inflorescence morphology are important genetic resources for the study on the genetic mechanisms underlying plant developmental programs and inflorescence architecture; they are also important for practical use to increase productivity. Normally, wheat spikelets are arranged in two parallel rows along the spike axis. The SCR (screwed spike rachis) lines represent a non-standard morphotype, which is characterized by a spiral arrangement of spikelets along the spiked rachis. The study of the early stages of the inflorescence development in SCR-lines using light and scanning electron microscopy revealed that the spiral arrangement of spikelets were not related to changes at the early stage of inflorescence development, and resulted from spiral growth of spike rachis cells at later stages of spike growth. Thus, the spiral arrangement of spikelets in cereal inflorescence may have resulted not only from peculiarities of the mutual arrangement of spikelet meristems (phyllotaxis), but also from cell growth features at later stages of inflorescence growth. It was shown that SCR is inherited as a dominant monogenic trait; its expression can be modified by genotypic background. The SCR-lines characterized using light and scanning electron microscopy represent an important genetic resource for further study of the molecular-genetic mechanisms determining plant architecture. Furthermore, they can be used to develop wheat lines and cultivars with new inflorescence phenotypes.

Overexpression of two PsnAP1 genes from Populus simonii × P. nigra causes early flowering in transgenic tobacco and Arabidopsis.

In Arabidopsis, AP1 is a floral meristem identity gene and plays an important role in floral organ development. In this study, PsnAP1-1 and PsnAP1-2 were isolated from the male reproductive buds of poplar (Populus simonii × P. nigra), which are the orthologs of AP1 in Arabidopsis, by sequence analysis. Northern blot and qRT-PCR analysis showed that PsnAP1-1 and PsnAP1-2 exhibited high expression level in early inflorescence development of poplar. Subcellular localization showed the PsnAP1-1 and PsnAP1-2 proteins are localized in the nucleus. Overexpression of PsnAP1-1 and PsnAP1-2 in tobacco under the control of a CaMV 35S promoter significantly enhanced early flowering. These transgenic plants also showed much earlier stem initiation and higher rates of photosynthesis than did wild-type tobacco. qRT-PCR analysis further indicated that overexpression of PsnAP1-1 and PsnAP1-2 resulted in up-regulation of genes related to flowering, such as NtMADS4, NtMADS5 and NtMADS11. Overexpression of PsnAP1-1 and PsnAP1-2 in Arabidopsis also induced early flowering, but did not complement the ap1-10 floral morphology to any noticeable extent. This study indicates that PsnAP1-1 and PsnAP1-2 play a role in floral transition of poplar.

Meristem identity and phyllotaxis in inflorescence development.

Inflorescence morphology is incredibly diverse. This diversity of form has been a fruitful source of inquiry for plant morphologists for more than a century. Work in the grasses (Poaceae), the tomato family (Solanaceae), and Arabidopsis thaliana (Brassicaceae) has led to a richer understanding of the molecular genetics underlying this diversity. The character of individual meristems, a combination of the number (determinacy) and nature (identity) of the products a meristem produces, is key in the development of plant form. A framework that describes inflorescence development in terms of shifting meristem identities has emerged and garnered empirical support in a number of model systems. We discuss this framework and highlight one important aspect of meristem identity that is often considered in isolation, phyllotaxis. Phyllotaxis refers to the arrangement of lateral organs around a central axis. The development and evolution of phyllotaxis in the inflorescence remains underexplored, but recent work analyzing early inflorescence development in the grasses identified an evolutionary shift in primary branch phyllotaxis in the Pooideae. We discuss the evidence for an intimate connection between meristem identity and phyllotaxis in both the inflorescence and vegetative shoot, and touch on what is known about the establishment of phyllotactic patterns in the meristem. Localized auxin maxima are instrumental in determining the position of lateral primordia. Upstream factors that regulate the position of these maxima remain unclear, and how phyllotactic patterns change over the course of a plant's lifetime and evolutionary time, is largely unknown. A more complete understanding of the molecular underpinnings of phyllotaxis and architectural diversity in inflorescences will require capitalizing on the extensive resources available in existing genetic systems, and developing new model systems that more fully represent the diversity of plant morphology.

A somaclonal line SE7 of finger millet (Eleusine coracana) exhibits modified cytokinin homeostasis and increased grain yield

The SE7 somaclonal line of finger millet (Eleusine coracana) achieved increased grain yield in field trials that apparently resulted from a higher number of inflorescences and seeds per plant, compared with the wild type. Levels of endogenous cytokinins, especially those of highly physiologically active iso-pentenyl adenine, were increased during early inflorescence development in SE7 plants. Transcript levels of cytokinin-degrading enzymes but not of a cytokinin-synthesizing enzyme were also decreased in young leaves, seedlings, and initiating inflorescences of SE7. These data suggest that attenuated degradation of cytokinins in SE7 inflorescences leads to higher cytokinin levels that stimulate meristem activity and result in production of more inflorescences. Gene expression was compared between SE7 and wild-type young inflorescences using the barley 12K cDNA array. The largest fraction of up-regulated genes in SE7 was related to transcription, translation, and cell proliferation, cell wall assembly/biosynthesis, and to growth regulation of young and meristematic tissues including floral formation. Other up-regulated genes were associated with protein and lipid degradation and mitochondrial energy production. Down-regulated genes were related to pathogen defence and stress response, primary metabolism, glycolysis, and the C:N balance. The results indicate a prolonged proliferation phase in SE7 young inflorescences characterized by up-regulated protein synthesis, cytokinesis, floral formation, and energy production. In contrast, wild-type inflorescences are similar to a more differentiated status characterized by regulated protein degradation, cell elongation, and defence/stress responses. It is concluded that attenuated degradation of cytokinins in SE7 inflorescences leads to higher cytokinin levels, which stimulate meristem activity, inflorescence formation, and seed set.

Wheat F-box protein recruits proteins and regulates their abundance during wheat spike development

F-box proteins, components of the Skp1-Cullin1-F-box (SCF) protein E3 ubiquitin ligase complex, serve as the variable component responsible for substrate recognition and recruitment in SCF-mediated proteolysis. F-box proteins interact with Skp1 through the F-box motif and with ubiquitination substrates through C-terminal protein interaction domains. F-box proteins regulate plant development, various hormonal signal transduction processes, circadian rhythm, and cell cycle control. We isolated an F-box protein gene from wheat spikes at the onset of flowering. The Triticum aestivum cyclin F-box domain (TaCFBD) gene showed elevated expression levels during early inflorescence development and under cold stress treatment. TaCFBD green fluorescent protein signals were localized in the cytoplasm and plasma membrane. We used yeast two-hybrid screening to identify proteins that potentially interact with TaCFBD. Fructose bisphosphate aldolase, aspartic protease, VHS, glycine-rich RNA-binding protein, and the 26S proteasome non-ATPase regulatory subunit were positive candidate proteins. The bimolecular fluorescence complementation assay revealed the interaction of TaCFBD with partner proteins in the plasma membranes of tobacco cells. Our results suggest that the TaCFBD protein acts as an adaptor between target substrates and the SCF complex and provides substrate specificity to the SCF of ubiquitin ligase complexes.

Characterization of Arabidopsis MYB transcription factor gene AtMYB17 and its possible regulation by LEAFY and AGL15

MYB transcription factors compose one of the largest transcription factor families in Arabidopsis, which play important roles in various developmental processes as well as defense responses against environmental stresses. In this study, we report the characterization of AtMYB17 gene, a putative R2R3 type MYB gene family member in Arabidopsis. AtMYB17 was found exclusively localized in nuclear, with an activation domain at its C-terminus. AtMYB17 was highly expressed in inflorescences and siliques, especially at early flower developmental stages. The level of AtMYB17 transcripts was also found to increase after imbibition during seed germination and gradually concentrate to the shoot apex. Bioinformatics analysis identified several binding sites of LEAFY (LFY) and AGL15 in the promoter region of AtMYB17. Promoter-GUS fusion analysis showed that the LFY binding sites were important in fine-tuning regulation of the spatio-temporal expression of AtMYB17 in transgenic plants. Moreover, AtMYB17 was up-regulated in 35S::AGL15 plants. Taken together, our data suggest that LFY may be involved in the regulation of AtMYB17, possibly together with AGL15, and thereafter in early inflorescence development and seed germination.

Conservation and divergence of APETALA1/FRUITFULL‐like gene function in grasses: evidence from gene expression analyses

Duplicated APETALA1/FRUITFULL (AP1/FUL) genes show distinct but overlapping patterns of expression within rice (Oryza sativa) and within ryegrass (Lolium temulentum), suggesting discrete functional roles in the transition to flowering, specification of spikelet meristem identity, and specification of floral organ identity. In this study, we analyzed the expression of the AP1/FUL paralogues FUL1 and FUL2 across phylogenetically disparate grasses to test hypotheses of gene function. In combination with other studies, our data support similar roles for both genes in spikelet meristem identity, a general role for FUL1 in floral organ identity, and a more specific role for FUL2 in outer floral whorl identity. In contrast to Arabidopsis AP1/FUL genes, expression of FUL1 and FUL2 is consistent with an early role in the transition to flowering. In general, FUL1 has a wider expression pattern in all spikelet organs than FUL2, but both genes are expressed in all spikelet organs in some cereals. FUL1 and FUL2 appear to have multiple redundant functions in early inflorescence development. We hypothesize that sub-functionalization of FUL2 and interaction of FUL2 with LHS1 could specify lemma and palea identity in the grass floret.

Comparison of Early Inflorescence Development between Japanese Pear (Pyrus pyrifolia Nakai) and Quince (Cydonia oblonga Mill.)

Japanese pear (Pyrus pyrifolia) and quince (Cydonia oblonga) form different inflorescence architectures, the former forms raceme inflorescence with about eight flowers and the latter forms solitary-flowered inflorescence. We observed the floral differentiation of Japanese pear and quince to clarify the differences in their early inflorescence development. Floral differentiation of Japanese pear occurred in late June. After apical meristem turned to a dome-like structure, inflorescence developed by forming lateral flower meristems in axils of bracts. The apex of inflorescence became the terminal flower meristem, two or three meristems were initiated in axils of outer bracts and leaf primordia, and finally approximately eight flower meristems were formed in the inflorescence. Floral differentiation of quince was initiated from late October to November after eight leaf primordia had been initiated. Apical meristem transformed to a dome-like structure and initiated sepal primordia. Axillary meristems of bracts or leaf primordia in Japanese pear differentiate to flower meristem while those in quince remained undifferentiated to form axillary buds in the following growing season, resulting in an inflorescence architecture difference in Japanese pear and quince.

Herbage production and animal performance on perennial ryegrass/white clover dairy pastures under alternative spring grazing managements

Spring grazing managements which allowed some early inflorescence development, followed by hard grazing in order to remove the reproductive stems (designated ‘late control’), were compared with a conventional close grazing strategy (‘early control’) in three paddock-scale complete randomized block studies with dairy cows grazing perennial ryegrass/white clover pastures during consecutive years (1990–1993) in Palmerston North, New Zealand. Experiment 1 was designed to evaluate the timing of late control, Expt 2 to investigate the most suitable intensity of control, and Expt 3 tested different practical approaches to carrying out the late control management.Late control generated an average herbage production increase of 24% (750 kg DM/ha) in October and November (spring) and 22% (950 kg DM/ha) from January to April (summer–autumn) compared with the conventional management. The increase in herbage accumulation during spring was a consequence of greater perennial ryegrass reproductive development. Increased summer–autumn herbage accumulation after late control management was attributed to enhanced tillering activity and accumulation of perennial ryegrass. Effects on white clover production were inconsistent. Experiment 3 compared the effects of two practical late control strategies, involving either a short period of rapid rotational grazing or a simulation of increased stocking rate normally associated with forage conservation, on the milk production and forage intake of groups of cows in self-contained farmlet systems. During the control period, the reproductive state of the sward caused a small decrease in herbage digestibility. However, the extra herbage produced subsequently did not differ in digestibility from that produced on the conventionally managed sward and resulted in an increase of around 11·5% in total milk solids yield (fat, protein and lactose) per cow, particularly towards the end of the season (March and April).The late control spring grazing management strategy is a viable option for dairy farmers. It should coincide with the time of anthesis of ryegrass plants (late November–early December), and is more effectively achieved when forage conservation practices are used to augment grazing pressure in the grazed area of the farm.

Inflorescence development in a new teosinte:Zea nicaraguensis(Poaceae)

Inflorescence development in a newly discovered teosinte, Zea nicaraguensis (Poaceae), from Nicaragua has been investigated using scanning electron microscopy (SEM). The SEM examination revealed that the pattern of both male and female inflorescence development was similar to previously described inflorescence in other Zea taxa. Branch primordia were initiated acropetally in a distichous pattern along the rachis of male and female inflorescences. Spikelet pair primordia bifurcated into pedicellate and sessile spikelet primordia. Predictably, pedicellate spikelet development was arrested and aborted in the female teosinte inflorescence. Organogenesis of functional spikelets and florets was similar to that previously described in maize and teosintes. The results were consistent with our hypothesis that both femininity and masculinity share a common mechanism of inflorescence development in Zea and Tripsacum and are in accord with a putative common mechanism of sex determination in the Andropogoneae. Interestingly, this population of teosinte, unique in its ability to grow in water-logged soils, showed a stable pattern of early inflorescence development. Our results also revealed the uncharacteristic presence of inflorescence polystichy in this population of Zea nicaraguensis. We propose this novel phenotypic variation raises the possibility that a domestic evolution of polystichy in maize was enabled by an occasional polystichous phenotypic in teosinte.

The Morphology of Staminate Flower Differentiation in Pecan

Abstract Inflorescence and staminate flower development in pecan [Carya illinoinensis (Wang.) K. Koch] were examined using scanning electron microscopy. Organogenesis was described from inception to pollen dehiscence. The order of organ initiation was: a single bract, rounded floral apex, 2 lateral bracteoles, and 3-7 stamens. The initiation and time when stages of floral differentiation occur were determined for 1 protandrous and 2 protogynous cultivars. The time of early inflorescence development and the initiation of floral primordia and bracts were similar in both cultivar types, occurring about 12 months prior to staminate maturity. However, the initiation time of later floral development stages was divergent. The floral stages in the protandrous cultivar up to anther lobing occurred during the previous growing season. In the protogynous cultivars, initiation of bracteoles, the floral apex, stamen primordia and anther lobing took place in the spring of their anthesis.