Objective To assess the value of joint detection of urinary neutrophil gelatinase-associated lipocalin(uNGAL), urinary N-Acetyl-β-D-glucosaminidase (uNAG), urinary kidney injury molecule 1(uKIM-1) for early diagnosis of acute kidney injury(AKI) in elderly patients. Methods A total of 184 elderly patients who stayed Taishan sanitarium between June 2016 and June 2018 were enrolled.According to acute kidney injury net(AKIN) work, the patients were divided into non-AKI group(68 cases)and AKI group(116 cases including 55 cases for stage 1, 39 cases for stage 2 and 24 cases for stage 3). The concentrations of urine NGAL, NAG, KIM-1 were measured.The diagnosis value of three biomarkers for AKl were analyzed with the receiver operating characteristic(ROC)curve and the area under curve(AUC). Results ①The levels of uNGAL, uNAG, uKIM-1 were higher in patients with AKI than the patients with no AKI(P<0.05). State 3 were higher than state 2 and state 1, state 2 were higher than state 1(P<0.05). ②The area under curves of uNGAL, uNAG, uKIM-1 were 0.734, 0.804 and 0.705 respectively.③The sensitivity and specificity of joint tests of three biomarkers were 84.9% and 90.7% respectively, higher than single test. Conclusions The urine NGAL, NAG and KIM-1 are sensitive indexes for the early diagnosis of elderly AKI.Joint detection has high value for early diagnosis of AKI. Key words: Acute Kidney Injury; Aged; Neutrophil gelatinase-associated lipocalin; Membrane Glycoproteins; Kidney injury molecule 1