Early Migration Research Articles

Ovarian Neuroglial Choristoma in a Bitch

Simple SummaryChoristomas are rare malformations consisting of normal mature tissue in an abnormal location. Neuroglial choristomas consist of heterotopic mature neural tissue and, in human medicine, they are predominantly reported in the head and in the neck, except for one recent case reported in a foot of a child. In domestic animals, neuroglial choristomas are exceedingly rare, reported only in the retina of a dog, in the pharynx and in the skin of two kittens, and within the oropharynx of a harbor seal. In this report we describe the first ovarian case of neuroglial choristoma in canine species. The lesion was present in a three-year-old intact female Jack Russell Terrier that, seven years after ovariectomy, is still clinically normal. Together with a recent case described in the foot of a child, this case confirms that neuroglial choristoma may also be found far from the skull or spine, supporting the hypothesis that they may arise from an early embryological migration defect.Neuroglial choristomas are rare malformations consisting of heterotopic mature neural tissue at a site isolated from the brain or spinal cord. In human medicine, neuroglial choristomas are predominantly reported in the head and in the neck, except for one recent case reported in a foot of a child. In domestic animals, neuroglial choristomas are exceedingly rare, reported only in the retina of a dog, in the pharynx and in the skin of two kittens, and within the oropharynx of a harbor seal. A three-year-old intact female Jack Russell Terrier presented for elective ovariectomy exhibited a cystic lesion 2 cm in diameter expanding in the right ovary. Histological examination of the lesion revealed a mass composed of well-organized neuroglial tissue. Immunohistochemistry with primary antibodies against GFAP, NSE, and IBA-1 confirmed the neuroglial origin of the mass. At the time of this writing, 7 years after ovariectomy, the dog was clinically normal. Together with a recent case described in the foot of a child, this case confirms that neuroglial choristoma may also be found far from the skull or spine, supporting the hypothesis that they may arise from an early embryological migration defect.

Short- and long-term outcomes of early initiation of peritoneal dialysis following catheter insertion.

Early-start peritoneal dialysis (PD) (use of PD catheter within 48 hours of insertion) is an innovative approach for prompt initiation of PD. This study was conducted to analyze the outcomes of early-start PD. A total of 100 patients on PD were retrospectively analyzed. Patients were grouped according to the "break-in period": < 48 hours (PD1) and ≥ 14 days (PD2). PD was initiated with low dwell volumes (500 mL) in a recumbent position within 48 hours of surgery. PD prescription was gradually incremented over 10 days to minimize any complications. In our study, there were 48 patients in the PD1 group and 52 in the PD2 group. The most common cause of end-stage kidney disease (ESKD) was diabetes mellitus in both groups. Incidence of early mechanical complications (within 30 days of catheter insertion), such as catheter obstruction, early catheter leakage, catheter malposition, tip migration, and infectious complications, were not found to be higher in the PD1 group. 1- and 4-year catheter patency rates were 97.0% and 96.2% in the PD1 group, respectively. These rates were comparable with those in the PD2 group. Early-start PD was not associated with an increased incidence of catheter failure (HR = 1.0, 95% CI 0.28 - 3.47). An early break-in period of < 48 hours is a feasible option for ESKD patients without any significantly increased risk of mechanical or infectious complications. It offers a safe and efficacious option for renal replacement therapy.

A Long-Term View of Refugee Flows from Ukraine: War, Insecurities, and Migration

This article examines the exodus of migrants from Ukraine in the context of the Russian invasion, making use of the conflict model of migration. We argue that Ukraine has long been characterized by insecurities which have already fueled large waves of emigration from the country. Indeed, the most recent phase of Ukrainian emigration should be seen as a continuation of the ongoing tendency of people residing in the country to seek escape from the overarching conditions of insecurity there. Earlier migrations from Ukraine have also established a culture of migration which has mediated the outflow of Ukrainians during the current crisis. Ukrainians who had already harboured inclinations to leave the country and who were possessed of the necessary capabilities (i.e. social capital, financial capital, human capital and physical ability) became mobile at the onset of the invasion in February, while many others were left behind. At the same time, the welcoming attitude of many European states has been key to the fast and safe exodus of Ukrainian migrants thus far.

Baltic Migrants in the Middle Dnipro Region: A Comparative Study of the Late Viking Age Archaeological Complex of Ostriv, Ukraine

THE LATE VIKING-AGE CEMETERY OF OSTRIV, located approximately 80 km south of Kyiv in the region along the Ros’ River, was discovered by the Institute of Archaeology of the National Academy of Sciences of Ukraine team in 2017. By 2020, 67 inhumation graves had been excavated in an area of 1400 sq m. Most of the artefacts from Ostriv are uncommon in Ukraine, but frequently found in the East Baltic region. This suggests a complex multi-ethnic population, presumably consisting of Baltic region migrants and local Slavs, and represents the easternmost example of its kind. Ostriv is a vivid example of how research on complex, multi-ethnic ancient populations benefits from multidisciplinary international collaborations. This article provides the first synthesis of the archaeological artefacts and burial rites from Ostriv, informed by the results of 14C-dating, stable isotopes, aDNA, physical anthropology and nondestructive metal analyses. It represents an important contribution to the renewed interest in early medieval migration, ethnic complexity and cultural encounters.

Factors that affect migratory Western Atlantic red knots (Calidris canutus rufa) and their prey during spring stagingon Virginia's barrier islands.

Understanding factors that influence a species' distribution and abundance across the annual cycle is required for range-wide conservation. Thousands of imperiled red knots (Calidris cantus rufa) stop on Virginia's barrier islands each year to replenish fat during spring migration. We investigated the variation in red knot presence and flock size, the effects of prey on this variation, and factors influencing prey abundance on Virginia's barrier islands. We counted red knots and collected potential prey samples at randomly selected sites from 2007-2018 during a two-week period during early and peak migration. Core samples contained crustaceans (Orders Amphipoda and Calanoida), blue mussels (Mytilus edulis), coquina clams (Donax variabilis), and miscellaneous prey (horseshoe crab eggs (Limulus polyphemus), angel wing clams (Cyrtopleura costata), and other organisms (e.g., insect larvae, snails, worms)). Estimated red knot peak counts in Virginia during 21-27 May were highest in 2012 (11,959) and lowest in 2014 (2,857; 12-year peak migration [Formula: see text] = 7,175, SD = 2,869). Red knot and prey numbers varied across sampling periods and substrates (i.e., peat and sand). Red knots generally used sites with more prey. Miscellaneous prey ([Formula: see text] = 2401.00/m2, SE = 169.16) influenced red knot presence at a site early in migration, when we only sampled on peat banks. Coquina clams ([Formula: see text] = 1383.54/m2, SE = 125.32) and blue mussels ([Formula: see text] = 777.91/m2, SE = 259.31) affected red knot presence at a site during peak migration, when we sampled both substrates. Few relationships between prey and red knot flock size existed, suggesting that other unmeasured factors determined red knot numbers at occupied sites. Tide and mean daily water temperature affected prey abundance. Maximizing the diversity, availability, and abundance of prey for red knots on barrier islands requires management that encourages the presence of both sand and peat bank intertidal habitats.

Agriculture and the Integration of British Colonial Migrants in Early Modern Ireland

Abstract This article offers a critical re-examination of Early Modern migrations to Ireland and their effect on farming practices, c.1580–1660. During and after the English conquest of Ireland, tens and eventually hundreds of thousands of settlers arrived from Britain. Focusing on Munster and to a lesser extent Ulster, I argue they were not greeted with an agricultural tabula rasa ripe for ‘improvement’. In contrast to what Tudor writers claimed, and what some scholars today have assumed, cereal cultivation and field enclosure already formed important elements in the agricultural landscape. Changes clearly took place, but English, Welsh and Scots settlers also made some remarkable adaptations by accepting local breeds of livestock and relying economically on forms of semi-mobile pastoralism that earlier writers had decried. Looking outside Ireland helps to evaluate their actions, since livestock mobility was widespread in contemporary European pastoralism, and if anything contributed to, rather than conflicted with, the commercialisation of farming.

Is it worth the risk? Clinical and radiographic outcomes 24 months after reverse shoulder arthroplasty in an advanced geriatric population

BackgroundThe application of reverse shoulder arthroplasty (RSA) has risen in the past decades especially due to its excellent long-term outcomes. With this positive trend, the indications for RSA have gradually extended to a broader age spectrum. The objective of this study was to identify the benefits of primary RSA in an advanced geriatric population with considerable comorbidity burden and higher perioperative risk.MethodsFor this observational study using data collected from our local RSA register, we identified 73 patients (77% female) with a minimum age of 85 years (range: 85-93 years) at the time of surgery and a complete 24-month postoperative follow-up. Clinical evaluations of pain, Subjective Shoulder Value, Constant score, Shoulder Pain and Disability Index, quality of life (European Quality-of-Life 5-Dimension 5-Level utility), and patient satisfaction were made. Radiographic evaluation followed an international consensus core set. Adverse events were documented according to a core event set.ResultsPreexisting medical conditions categorized following the American Society of Anesthesiologists physical status classification system indicated only 22% of patients with mild comorbidities (American Society of Anesthesiologists I-II), whereas severe (American Society of Anesthesiologists III-IV) comorbidities were common (78%). Indications for surgery were rotator cuff deficiency (72%), post-traumatic conditions (18%), and primary arthrosis (10%). There was significant improvement in all clinical evaluations up to 24 months post RSA: mean pain levels decreased from 6.2 to 1.6 points, where 0 indicates no pain (P < .001) and Subjective Shoulder Value, Constant score, Shoulder Pain and Disability Index, and European Quality of Life 5 Dimensions 5 Level increased from 36% to 76%, 26 to 61 points, 29 to 74 points, and 0.58 to 0.79, respectively (P < .001). Most patients (88%) opted in favor of undergoing the same surgery again based on their personal outcome. There were no signs of early loosening, migration or dislocation at 24 months postsurgery. However, 6 periprosthetic fractures were identified, 5 of which were treated conservatively. Adverse events were reported for 39% of patients, yet rarely led to the need for revision surgery (1.8%) or hospital readmission (3.6%).ConclusionDespite an advanced age over 85 years and numerous associated comorbidities, our geriatric population showed a distinct clinical improvement in their daily activities with high rates of patient satisfaction. Radiographic analysis at 24 months after surgery identified adequate implant stability. RSA is a safe procedure, even in these elderly patients, with an acceptable risk of unfavorable medical and surgical events.

Prenatal hypoxia alters the early ontogeny of dopamine neurons

Dopaminergic (DA) dysfunction is a significant feature in the pathophysiology of schizophrenia. Established developmental risk factors for schizophrenia such as maternal immune activation (MIA) or developmental vitamin D (DVD) deficiency, when modelled in animals, reveal the differentiation of early DA neurons in foetal brains is delayed suggesting this may be a convergent aetiological pathway. Here we have assessed the effects of prenatal hypoxia, another well-known developmental risk factor for schizophrenia, on developing DA systems. Pregnant mice were exposed to a hypoxic environment of 10% oxygen for 48 h from embryonic day 10 (E10) to E12. Embryonic brains were collected and the positioning of mesencephalic cells, expression of DA specification and maturation factors were examined along with the expression of factors that may govern the migration of these neurons. We show that prenatal hypoxia results in a decrease in dopaminergic progenitors retards early DA neuron lateral migration and reduces expression of the receptors known to govern this process. A second time-point, postnatal day 10 (P10) was also examined in order to assess whether prenatal hypoxia alters early presynaptic architecture in the developing striatum. We show reduced expression of tyrosine hydroxylase (TH) in the postnatal striatum along with increases in the density of high-probability DA release sites within TH varicosities. These findings add to the emerging literature showing that multiple epidemiologically validated environmental risk factors for schizophrenia may induce early alterations to develop DA systems. This may represent a possible convergent mechanism in the onset of presynaptic DA dysfunction in patients.

Electrochemically applied hydroxyapatite on the cementless porous surface of Bi-Metric stems reduces early migration and has a lasting effect : an efficacy trial of a randomized five-year follow-up radiostereometric study.

BoneMaster is a thin electrochemically applied hydroxyapatite (HA) coating for orthopaedic implants that is quickly resorbed during osseointegration. Early stabilization is a surrogacy marker of good survival of femoral stems. The hypothesis of this study was that a BoneMaster coating yields a fast early and lasting fixation of stems. A total of 53 patients were randomized to be treated using Bi-Metric cementless femoral stems with either only a porous titanium plasma-sprayed coating (P group) or a porous titanium plasma-sprayed coating with an additional BoneMaster coating (PBM group). The patients were examined with radiostereometry until five years after surgery. At three months, the mean total translation (TT) was 0.95 mm (95% confidence interval (CI) 0.68 to 1.22) in the P group and 0.57 mm (95% CI 0.31 to 0.83) in the PBM group (p = 0.047). From two to five years, the TT increased by a mean of 0.14 mm (95% CI 0.03 to 0.25) more in the P group than in the PBM group (p = 0.021). In osteopenic patients (n = 20), the mean TT after three months was 1.61 mm (95% CI 1.03 to 2.20) in the P group and 0.73 mm (95% CI 0.25 to 1.21) in the PBM group (p = 0.023). After 60 months, the mean TT in osteopenic patients was 1.87 mm (95% CI 1.24 to 2.50) in the P group and 0.82 mm (95% CI 0.30 to 1.33) in the PBM group (p = 0.011). There was less early and midterm migration of cementless stems with BoneMaster coating compared with those with only a porous titanium plasma-sprayed coating. Although a BoneMaster coating seems to be important for stem fixation, especially in osteopenic patients, further research is warranted. Cite this article: Bone Joint J2022;104-B(6):647-656.

Preceramic riverside hunter-gatherers and the arrival of Neolithic farmers in northern Luzon

The most westerly Pacific island chain, running from Taiwan southwards through the Philippines, has long been central in debates about the origins and early migrations of Austronesian-speaking peoples from the Asian mainland into the islands of Southeast Asia and Oceania. Focusing on the Cagayan Valley of northern Luzon in the Philippines, the authors combine new and published radiocarbon dates to underpin a revised culture-historical synthesis. The results speak to the initial contacts and long-term relationships between Indigenous hunter-gatherers and immigrant Neolithic farmers, and the question of how the early speakers of Malayo-Polynesian languages spread into and through the Philippines.

Impact of a new conceptualized anti-reflux Trumpet stent on the quality of life of patients with advanced carcinoma of the cardio-oesophageal junction.

To evaluate a newly developed, self-expandable anti-reflux Trumpet (ART) stent customized for cardio oesophageal junctional (COJ) cancer on the feasibility of deployment, stent migration, quality of life, and symptom relief. Prospective case series, Proof of concept pilot study. Tertiary Health Care Center, Hospital Tuanku Jaafar, Seremban, Malaysia. Department of Surgery. A total of 17 patients diagnosed with advanced COJ tumour and who had never undergone any surgical, endoscopic, or chemoradiotherapy and indicated for stenting were recruited. The study period was over nine months, and follow-up was one-month post-stenting. Endpoint measures were feasibility of deployment of the new design, symptoms relief, early stent migration, early complication, GERD Q score, and (QOL)assessment. The ART stent was inserted successfully in all cases (17/17, 100%). There were two stent migrations due to the flexibility of the stent at the neck. There were no early or post-stenting one-month complications associated with the procedure. A good flow of contrast was seen in all the stents deployed. GERD Q score was low in all patients pre and post-stenting. Post-stenting there was a relief of dysphagia, weight gain, and a 60% improvement in QOL score. ART stent is feasible and technically successful in COJ tumours. It provides good symptom relief, improves the QOL, and has minimal early complications. None declared.

The origins of multi-cropping agriculture in Southwestern China: Archaeobotanical insights from third to first millennium B.C. Yunnan

Yunnan’s location at the crossroad of temperate China, Northeast India and tropical mainland Southeast Asia makes it a pivotal area for the understanding of early cultural contacts and agricultural spread between these ecologically diverse regions. This paper evaluates current evidence relating to the emergence of the first agricultural systems in Yunnan. It also reviews previous theories on agricultural dispersal to Yunnan, including whether Austroasiatic speakers were responsible for the spread of rice from Yunnan to mainland Southeast Asia, and builds a new framework that allows to tie agricultural development in the region into broader patterns of early migration and exchange networks. Archaeobotanical remains attest to an initial spread of rice and millet from Central China into Yunnan in the third millennium B.C. and the establishment of a mixed-crop economy; the introduction of wheat and barley in the second millennium B.C. allowed for increased diversification of the agricultural system, with a two-season intensification trend in the late first millennium B.C. Differences in early rice cultivation ecologies between Yunnan and mainland Southeast Asia suggest that Yunnan rice farmers may not have had a primary role in the southern dispersal of rice, however, more data is needed to fully clarify the source and development of dryland cultivation of rice in mainland Southeast Asia.

Early root migration after a mandibular third molar coronectomy

PurposeThis prospective cohort study aimed to assess early root migration after a coronectomy of the mandibular third molar at 2 and 6 months after surgery.MethodsWe included all patients treated with a coronectomy of an impacted mandibular third molar. The primary outcome measure was the extent of postoperative root migration after 2 and 6 months. Migration was measured as the distance between the root complex and a fixed point on the inferior alveolar canal. The secondary aim was to identify factors (age, impaction pattern, and patient sex) that affected the extent of root migration.ResultsOne hundred and sixty-five coronectomies were performed in 141 patients (96 females and 45 males; mean age 33.1 years, SD 16.0). The 2-month checkup was completed by 121 patients that received 141 coronectomies. The 6-month check-up was completed by 73 patients that received 80 coronectomies. The mean root migrations were 3.30 mm (SD 2.53 mm) at 2 months and 5.27 mm (SD 3.14 mm) at 6 months. In the 2–6-month interval, the mean root migration was 2.58 mm (SD 2.07 mm). The extents of migration were similar during the 0–2-month interval and the 2–6-month interval (p = 0.529). Younger age was associated with greater root migration, and females experienced significantly greater migrations than males (p = 0.002).ConclusionRoots migrated more rapidly in the first two postoperative months, compared to the 2–6-month interval. Age was negatively correlated with the extent of root migration, and females showed significantly greater migrations than males.

Prolonged early migration of dust from the inner Solar System to the comet-forming region

The most abundant group of meteorites currently falling to Earth, ordinary chondrites, originate from S-type (Si-rich) asteroids and are thought to have originated in the inner Solar System. These asteroids typically underwent only minor aqueous alteration but experienced varying degrees of thermal metamorphism that altered their primary compositions and textures. However, some rare members remain unaltered and retain the pristine compositions they obtained in the protoplanetary disk prior to accretion of their parent asteroids. In contrast, comets formed in the icy reaches of the outer Solar System. Here we report on silicate minerals in pristine ordinary chondrites that are compositionally distinct from those in all other known chondrites but show similarities to those found in comet samples returned from Comet Wild 2 by NASA's Stardust mission and those sourced from an unknown number of comets represented by interplanetary dusty particles. The identification of this material suggests that comets may have formed from diverse far-flung Solar System materials, including grains that migrated from the inner Solar System to the comet-forming region between ∼1 Myr and potentially ⪆3 Myr after the first Solar System solids formed. This finding suggests that migration from the inner to the outer Solar System lasted for millions of years and that comets are composed of residual materials from the entire early Solar System.

Birth of the Mexican Problem: Oil in Mexico, U.S. Social Sciences, and Transnational Labor, 1917–1920

Abstract Birth of the Mexican Problem is a historian’s version of a postcolonial analysis. I use the narrative history and research materials of one of the earliest think tanks in U.S. history to show how and why Mexico was made legible to U.S. audiences. Not just a response to early twentieth-century Mexican migration, the so-called Mexican problem was also deeply tied to U.S. capitalists’ economic interests in Mexico. I argue for the centrality of ties between big business, academics, and government in constructing ideas about Mexico and Mexican transnational labor. The story begins with an oilman named Edward Doheny.

Modelling past migrations to determine efficient management rules favouring silver eel escapement from a large regulated Floodplain Lake

As human activities caused a dramatic decline of European eel population since the 1970 s, the European Union has set targets to ensure a 40% escapement to the sea of the silver eel biomass by considerably reducing anthropogenic impact. Thus, human obstacles to fish migration like dams and hydropower plants should enable efficient management measures ensuring safe passage for eels during the migration. In order to provide a quick and efficient assessment of eel conservation measure applied to the sluice gates of a large floodplain lake, we implemented a novel evaluation method using predictions on past migration seasons when no management measure were applied. For this purpose, we collected acoustic telemetry monitoring data over three migration seasons and fitted a predictive model based on Boosted Regression Trees (BRTs) to describe the influence of environmental parameters on migration. The water level difference over two days proved to be decisive, along with early migration occasions in the season, as an increase of at least 10 cm water level was associated with a sharp increase of migration probability. We then used the BRT model to predict migration occasions at the dam over 8 past seasons and forecast the impact on escapement of management measures if they had been applied. Thanks to this original prospective assessment of dam management measures, we identified an enhanced opening decision rule, capable of increasing the eel escapement for each year of the study. The management measure is particularly efficient during the years with poor hydrological conditions (i.e. droughts), that is the periods with the lowest initial escapement rates. Finally, efficient management measures to increase silver eel escapement were based on increasing the number of gate opening days by only a few days per year (c.a. 15%). However, the management measure only focused on silver eel migration, so that further investigations should consider the impacts of other threats occurring during the life cycle. This study also provides a highly operational approach for fast evaluation of conservation measures, avoiding lengthy and expensive monitoring campaigns of classical ex-post assessments.

Single Cell transcriptomes highlight the temporal hierarchy of fate decisions of the developmental tree from Neural Border Stages to Migration of NC Cells

Gene regulatory networks govern the steps of embryonic development and control cell transcriptional states. The neural crest cells are an archetypal example of cellular diversification from a multipotent progenitor population. However, the full sequence of molecular choices governing the emergence of neural crest heterogeneity from the ectoderm state remains elusive. Here we combine ultra‐dense single cell transcriptomes with machine‐learning strategies and experimental validation to provide a comprehensive gene regulatory network driving vertebrate neural crest fate diversification from induction to early migration stages. Transcription factor connectome and bifurcation analyses discover that several early neural crest fates emerge at the neural plate stage, alongside an unbiased multipotent neural crest lineage persisting until after epithelial‐mesenchymal transition. We also define a new and transient neural border zone state, preceding choice between neural crest and placodes during gastrulation. Our comprehensive experimental approach combined to several ML strategies broadly applicable to single cell transcriptomics deciphers the circuits driving cranial and vagal neural crest formation and provides a general model for investigating vertebrate GRNs in development, evolution and disease

Comparison of early migration patterns between a ceramic and polyethylene liner in uncemented Trabecular Titanium cups: a 2-year randomized controlled trial of 52 hips using radiostereometric analysis.

Background and purposeCeramic liners may reduce early stability of uncemented acetabular components due to higher stiffness. However, the bone ingrowth capacities of porous trabecular titanium might compensate for this effect. This prospective randomized trial quantifies migration patterns of the Delta-TT cup, and compares polyethylene and ceramic liners.Patients and methodsPatients undergoing primary uncemented total hip arthroplasty with the Delta-TT cup and femoral stem with ceramic head were randomized to a polyethylene (n = 25) or ceramic (n = 28) liner. Radiostereometric analysis (RSA) radiographs, patient-reported hip function (HOOS-PS, OHS), and quality of life (EQ5D) were collected at baseline and 1.5, 3, 6, 12, and 24 months postoperatively. Model-based RSA was used to calculate 3D cup translation and rotation, and mixed models were used to compare effects over time between groups.ResultsAt 2 years follow-up, Delta-TT cups showed similar mean proximal translation of 0.56 mm (95% CI 0.38– 0.75) in the ceramic (CE) group and 0.54 mm (0.30–0.77) in the polyethylene (PE) group, with a between group effect of 0.02 mm (–0.20–0.23). Most cup migration occurred in the first 1.5 to 3 months, stabilizing within 6 months. Any between-group effects were ≤ 0.30 mm for translation and ≤ 0.45° for rotation. Improvements in patient-reported hip function and quality of life were similar in both groups.InterpretationRegardless of liner type, Delta-TT cups showed some initial migration and stabilized within 6 months, which seems promising for longterm fixation in both cup-liner constructs.

Naturally Acquired Antibodies to Influenza A Virus in Fall-Migrating North American Mallards.

Although waterfowl are the primary reservoir for multiple subtypes of influenza A virus (IAV), our understanding of population immunity in naturally infected waterfowl is poorly understood. Population immunity may be an important driver of seasonal subtype predominance in waterfowl populations and may affect the potential for establishment of introduced IAV such as the Eurasian-like A/Goose/Guangdong/1/1996 lineage in these populations. Here, we examine the prevalence of naturally acquired antibodies to nucleoprotein (NP), hemagglutinin (H3, H4, H5), and neuraminidase (N1, N2, N6, N8) in early migrating mallards (Anas platyrhynchos) sampled in Northwest Minnesota during staging and early fall migration in September 2014, 2015, 2017, and 2018. Serologic results were compared to historic and contemporary virus isolation results from these same study sites. The prevalence of antibodies to NP ranged from 60.8–76.1% in hatch-year (HY) birds and from 86.0–92.7% in after-hatch-year (AHY, >1-year-old) mallards indicating a high level of previous infection with IAV early in the fall migration season. Neutralizing antibodies were detected against H3, H4, and H5 in all years as were antibodies to N1, N2, N6, and N8. A high proportion of NP seropositive ducks tested positive for antibodies to multiple HA and NA subtypes, and this was more common in the AHY age class. Antibody prevalence to the HA and NA subtypes included in this study were consistent with the predominance of H4N6 in these populations during all years and reflected a broadening of the antibody response with age. Additional work is needed to document the longevity of these immune responses, if and how they correlate with protection against IAV transmission, infection, and disease, and if, as detected in this study, they adequately describe the true extent of exposure to IAV or specific HA or NA subtypes.

Feasibility of Conversion of a New Bariatric Fully Endoscopic Bypass Procedure to Bariatric Surgery: a Porcine Pilot Study.

Bariatric endoscopic procedures are emerging as alternatives to bariatric surgical procedures. This study aimed to assess if a natural orifice transluminal endoscopic surgery (NOTES) bariatric procedure could be converted to a surgical duodenal-jejunal bypass (DJB) or sleeve gastrectomy (SG). This 12-week prospective study compared 4 test pigs to 3 control (no procedures) pigs aged 3months at baseline. The test pigs received a fully endoscopic NOTES-based bypass including measurement of the bypassed limb and creation of a gastrojejunal anastomosis (GJA) using gastrojejunal lumen-apposing metal stents (GJ-LAMS) at Week 0, placement of a duodenal exclusion device (DED) at Week 2, and randomization to DJB or SG surgery at Week 8 with subsequent 4-week follow-up. At Week 12, the pigs were sacrificed and necropsy was performed. Endoscopic procedures were technically successful. One pig did not receive a DED due to early GJ-LAMS migration leading to premature closure of the GJA. At Week 8, all 4 pigs were doing well, and the remaining 3 GJ-LAMS and 3 DEDs were uneventfully endoscopically removed. Two one-anastomosis DJB were performed, and 2 SG were performed, closing in one case the site of the previous GJA. The surgical procedures were technically feasible and uneventful during follow-up. Necropsy assessments showed no local or peritoneal inflammation or abscess and no leakage or fistula. An endoscopic bariatric bypass can be transitioned to a one-anastomosis duodenal-jejunal bypass or sleeve gastrectomy, without complications.