EAC Cells Research Articles

Reaction of Sheep Serum γ, β- and α-Globulins with Third Stage Larvae of Haemonchus contortus

Rabbit and guinea-pig antisera produced against Haemonchus contortus third stage larvae treated with scrum from ‘parasite-free’ sheep or parasitised sheep, contained antibodies that precipitated, in Immunoelectrophoresis (IEP), sheep slow andfast γ-globulin, Igm and up to 4 β-globulin and 3 α-globulin proteins. Serologically these antisera reacted with human erythrocytes carrying sheep antibody (anti-globulin testing) as well as sheep erythrocyte-rabbit amboceptor complexes treated with foetal lamb serum (EAC cells) as a source of complement (C). Evidence is presented that indicates that the β-and α-globulins, most of which are present in foetal lamb serum, may be components of the sheep C system.

Antineoplastic Activity of Bis-tyrosinediaqua Ni(II) Against Ehrlich Ascites Carcinoma

In order to find out new compounds having antineoplastic activity, bis-tyrosine diaqua nickel (II) complex was synthesized and characterized. The antineoplastic activity of the compound was judged by measuring inhibition of tumour growth and enhancement of survival time of EAC cell bearing mice. In addition its effects on haematological parameters and serum alkaline phosphatase activity of both normal and EAC bearing mice were also studied. The test compound significantly inhibited the tumour growth and increased life span of tumour bearing mice at dose 10 mg/kg i.p. The treatment also recovered the perturbed haematological parameters as well as the serum alkaline phosphatase activity towards normal. The host toxicity of the test compound was found to be negligible. The compound can be considered as an effective antineoplastic agent. Key words: Antineoplastic activity, Bis-tyrosinediaqua nickel (II), EAC, ALP activity  DOI = 10.3329/dujps.v7i1.1215 Dhaka Univ. J. Pharm. Sci. 7(1): 33-37, 2008 (June)

Effects of Ehrlich ascites tumor cells on the grouping behavior of dissociated normal cells

This study is concerned with contact and intercellular relationships of dissociated embryonic chick cells comingled with Ehrlich ascites tumor cells and aggregated by the rotation procedure. The capacity of the anaplastic malignant cells to become included in aggregates with embryonic cells was restricted to a variant fraction of this particular ascites tumor, comprising approx. 40 to 60 per cent of the respective tumor cell populations employed. The ability of these variant cells to adhere slightly to embryonic cells accounted for the effects on aggregation and histogenetic regrouping of embryonic cells in suspensions with tumor cells. The special properties of this tumor cell fraction were also evidenced by their capacity to form multicellular clusters in the absence of embryonic cells; however, these were seen macroscopically, only after an adaption period of at least 3 to 4 days in the rotating flask cultures. In the composite aggregates there was an impairment of the sorting out process and of the formative grouping of embryonic cells. This was not only due to the activity of the tumor cells, but also to the properties of the embryonic cells, particularly, to their adhesiveness relative to the tumor cells. This also varied with the type, activity and state of preservation of the embryonic cells. The tumor cells established preferential adhesions with the mesenchymal cells, particularly with the young, connective tissue elements. The tumor cells tended to form groupings within the stromal tissue components in the various types of composite aggregates that were studied. The frequency of inclusion of the tumor cells within reconstructed epithelial tissue was low; when it occurred, it probably represented changes in the surface properties of the epithelial cells due to the decline in viability or experimental treatment. The rate of processes involved in histogenetic regrouping and the effectiveness of histodifferentiation varied with the concentration ratio of EAC and embryonic cells in the original suspension mixture and the combinations of the cell populations. Histogenetic regrouping of reaggregating embryonic cells depended on critical, proportionate concentrations of the various embryonic cells and the tumor cells in the early phases of the process. The reconstructed tissue provided a microenvironment in which small numbers or even single tumor cells became established and could proliferate.

Virus absorption by tumor cells.

SummaryFresh preparations of the WS strain of influenza A virus were poorly absorbed by Ehrlich tumor cells but heated WS virus formed a loose union dissociable by treatment with RDE. The neurotropic variant of this virus (NWS), which is capable of multiplying in transplanted Ehrlich ascites tumor, readily combined with EAC cells and did not elute spontaneously. RDE and sodium periodate destroyed the tumor cell receptors of NWTS virus. However, NVVS virus hemagglutinin could not be liberated by RDE after interaction with intact EAC cells at 37°C for 105 minutes. This “fixation” of NWS virus was not demonstrable after shorter periods of incubation, at temperatures of 23°C and 1°C, or if the tumor cells were previously heated to 56°C.