To fulfill the demand of teaching and learning quality in Higher Education, different means of evaluating, assessing, and accrediting academic programs have evolved. The need arises on finding scientific tools to measure and assess quality at different stages of educational processes. In Higher Education, course portfolio is considered one of essential quality assurance tools used. It is used to monitor and develop activities, to help students construct knowledge, and to improve the academic activities. This paper tackles the effectiveness of such tool for improving learning and teaching processes College of Health and Sport Sciences, University of Bahrain. The results of this study showed that the college faculty have a positive perceptions towards the use of course portfolio. They also, positively perceive the usefulness of audit results of the course portfolio and show good intention towards using electronic course portfolio; however, they need more training and support to use it effectively. In this study, the benefits of course portfolio as an independent variable was found to be a significant predictor of e-portfolio acceptance. College of Health and Sport Sciences need to improve the implementation of e-portfolio system through continuous faculty feedback and improvement plans.