This study aims to explore the factors influencing the adoption of e-learning platforms in biology education and examine the impact of online learning on students’ performance. This study investigates the relationships between perceived usefulness, perceived ease of use, attitude toward e-learning, flexibility, content quality, and students’ behavioral intention to adopt e-learning activities. A mixed-methods approach was employed consisting of two phases: a questionnaire survey with structural equation modeling (SEM) to analyze data and an experiment with an independent sample t-test to assess the impact of online learning on student performance. Findings disclosed that perceived usefulness, perceived ease of use, attitude toward e-learning, flexibility, and content quality positively impacted students’ behavioral intention to adopt e-learning and their performance. This study contributes to the theoretical understanding of the factors influencing e-learning adoption in biology education. Practical recommendations are provided for educators, instructional designers, and policymakers to facilitate the implementation of e-learning platforms in biology education. These recommendations include promoting the perceived usefulness and ease of use of e-learning platforms, fostering a positive attitude toward e-learning, enhancing flexibility, ensuring high-quality content, providing training and support for educators, and considering the needs of students with disabilities. Doi: 10.28991/ESJ-2023-07-06-014 Full Text: PDF
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