Dynamic Turnover Research Articles

Patient safety in a rural sub-Saharan Africa hospital: A 7-year experience at the AIC Kijabe Hospital, Kenya

The development of a safety culture is challenging, primarily because it often disrupts institutional attitudes, norms and values. In the healthcare industry, most of the data on the results of unsafe care come from High-Income Countries. The Hospital Survey on Patient Safety Culture (HSOPS) is a tool for assessing, building, sustaining and comparing institutional safety cultures within healthcare organizations. We used the HSOPS over a 7-year period, and herein report our experience. The authors report their experience using the HSOPS tool in Kijabe Hospital, an institution with 650 employees, over a 7-year period. The HSOPS tool, with no local modifications, was distributed to all employees during each survey. The institutional HSOPS percent positive dimension scores for 2015, 2017 and 2019 were compared with baseline data from the 2013 survey. The average response rate during the study period was 84.5% (range 65.1% to 93.6%). In general, the mean percentage positive dimension scores of most domains improved in the 2019 survey (p<0.05), including reduced staff turnover and, improved hospital support for patient safety (p<0.0001), amongst other domains. The overall patient safety grade (excellent/very good), was 50% (range 43–64%). Although the dynamics of high staff turnover and hospital leadership change presented challenges in developing and measuring institutional patient safety culture, this study demonstrates that patient safety ideals can be developed and embraced in sub-Saharan Africa. Patient safety champions, a generative institutional leadership that is supportive of patient safety, are important for the development of an institutional safety culture. Creating an institutional just culture creates a patient safety culture.

A triadic model of job retention and turnover dynamics in the hospitality industry

Purpose This study aims to examine key factors influencing hospitality employee turnover in a post-pandemic context, challenging conventional assumptions about the role of demographics and work-related factors in retention decisions. Design/methodology/approach The research adopted a comprehensive framework using the capability approach and geospatial data analysis, integrating social vulnerability indexes with survey responses from 797 hospitality employees. This study analyzed turnover intentions across demographics, job roles and regions, focusing on Florida’s I-4 corridor. Findings Individual conversion factors like age and marital status were the strongest predictors of turnover, with older and married employees less likely to leave their jobs. In contrast, traditional variables like income, education and job type did not significantly influence turnover intentions. The pandemic blurred distinctions between job roles, highlighting personal circumstances, health risks and economic pressures as critical factors. Contrary to expectations, financial assistance did not significantly reduce turnover intentions. In addition, employees’ life circumstances, such as social vulnerability, influence labor relations and turnover more than work-related conditions. Research limitations/implications This study enriches turnover literature by confirming that personal life circumstances, like age and marital status, are pivotal in understanding employee retention. It challenges conventional demographic and work-related predictors and urges future research to explore the interaction between personal and professional factors in the hospitality industry. The study’s agent-based framework deepens the understanding of how various factors shape employee decisions to stay or leave. Practical implications The findings suggest that hospitality employers must develop more localized, employee-centric retention strategies, especially for younger employees. Tailored approaches considering regional and demographic differences, such as providing career development opportunities and flexible work conditions, could foster long-term loyalty. In rural areas, retention efforts should focus on improving job satisfaction and community support, while in urban areas, strategies prioritize career progression and flexibility. Social implications The study’s findings have significant social implications, particularly in reshaping how employee retention strategies are developed in the hospitality industry. By emphasizing life circumstances over traditional work-related factors, the research highlights the importance of supporting employees’ resilience, especially for those facing social vulnerability. Employers may need to create more flexible and inclusive policies that account for personal, economic and health-related challenges. In addition, the findings suggest that financial aid alone is insufficient in reducing turnover, calling for a more holistic approach that integrates emotional and social support to foster a more stable and loyal workforce. Originality/value This study challenges traditional turnover models by shifting focus from work-related factors to life circumstances, particularly the resilience of older and married employees. It integrates three dimensions – personal (sociodemographics), social (support) and environmental (job type, pandemic challenges) – to examine their influence on employee agency. This triadic framework offers insights into how individual, social and contextual factors shape turnover decisions.

Economic development and foreign trade: a сomparative analysis of the United States and China

The article presents a comparative analysis of socio-economic and foreign trade relationships between the United States and the People's Republic of China, two leading countries of the world economy, which exert maximum influence on global processes in economics and politicsas well as in world trade, investment activities and scientific and technical sphere. Today, the rivalry between the two powers has also intensified in the monetary and financial aspect due to the growing importance and share of the yuan in international settlements and government reserves of many countries. This situation continues to worsen smoothly, often having a negative impact on other countries, including the Russian Federation. The purpose of the article is to explore the current state of the demographic situation, the dynamics of GDP and trade turnover of the two countries, to characterize the current situation and identify trends in its development in the medium term, which will make it possible to form the positions and policies of other subjects of the world economy to ensure their stability and balance their actions. The article concludes that both countries are currently experiencing a period of socio-economic and foreign trade turbulence, and its range has not yet shown a tendency to decrease. It is often the United States that acts as the source of this turbulence, trying to overcome the negative processes in its own economy. Therefore, it is important for third countries, for example, Russia, to take consistent measures to ensure national, including foreign economic, security. The methodological basis of the research is statistical, comparative, graphical analysis, the study of reports and archives, calculations based on data from open international statistical databases and official websites of international economic organizations, the main purpose of which is to study the economic indicators of the United States and the People's Republic of China. The study period covers 2012–2023.

TRPC3-mediated NFATc1 calcium signaling promotes triple negative breast cancer migration through regulating glypican-6 and focal adhesion.

Canonical transient receptor potential isoform 3 (TRPC3), a calcium-permeable non-selective cation channel, has been reported to be upregulated in breast cancers and a modulator of cell migration. Calcium-sensitive transcription factor NFATc1, which is important for cell migration, was shown to be frequently activated in triple negative breast cancer (TNBC) biopsy tissues. However, whether TRPC3-mediated calcium influx would activate NFATc1 and affect the migration of TNBC cells, and, if yes, the underlying mechanisms involved, remain to be investigated. By immunostaining followed by confocal microscopy, TNBC lines MDA-MB-231 and BT-549 were both found to express TRPC3 on their plasma membrane while ER+ line MCF-7 and HER2+ line SK-BR3 do not. Blockade of TRPC3 by pharmacological inhibitor Pyr3 or stable knockdown of TRPC3 by lentiviral vector both inhibited cell migration as measured by wound healing assay. Importantly, blocking TRPC3 by Pyr3 or knockdown of TRPC3 both caused the translocation of NFATc1 from the nucleus to the cytosol as revealed by confocal microscopy. Interestingly, NFATc1 was found to bind to the promoter of glypican 6 (GPC6) as determined by chromatin immunoprecipitation assay. Consistently, knockdown of TRPC3 decreased the expression of GPC6 as revealed by western blotting. Moreover, long-term knockdown of GPC6 by lentiviral vector also consistently decreased the migration of TNBC cells. Intriguingly, GPC6 proteins physically interact with vinculin in MDA-MB-231 as determined by co-immunoprecipitation. Blockade of TRPC3, knockdown of TRPC3 or knockdown of GPC6 all induced larger, stabilized actin-bound peripheral focal adhesion (FA) formations in TNBC cells as determined by co-staining of actin and vinculin followed by confocal microscopy. These large, stabilized actin-bound peripheral FAs indicated a defective FA turnover, and were reported to be responsible for impairing directed cell migration. Our results suggest that, in TNBC cells, calcium influx through TRPC3 channel positively regulates NFATc1 nuclear translocation and GPC6 expression, which maintains the dynamics of FA turnover and optimal cell migration. Our study reveals a novel TRPC3-NFATc1-GPC6-vinculin signaling cascade in maintaining the migration of TNBC cells.

Modulation of fatty acid profiles and turnover dynamics in jellyfish polyps through copepod diets: Insights into trophic interactions and nutrient flux.

Fatty acids (FAs) are vital biomolecules crucial for determining food quality for higher trophic levels. To investigate FA transfer and turnover time in predators, we conducted a diet switch experiment using jellyfish polyps. These polyps were fed food sources including Artemia sinica nauplii and FA-manipulated copepod Pseudodiaptomus annandalei, maintained on distinct algal diets with varied FA compositions. Our findings reveal that copepods may have a strong potential to synthesize long-chain polyunsaturated FA to maintain biochemical homeostasis when consuming low-quality food. Consequently, the species-specific fatty acid composition within plankton, combined with effects of seasonal environmental fluctuations and climate change, leads to changes in the FA composition of foundational food web components. These alterations create a complex "nutrient black box" effect as they propagate up trophic levels. Our study shows that jellyfish polyps fail to accumulate EPA and DHA but display high levels of ARA compared to their zooplankton and phytoplankton food sources, suggesting a potential association with dietary EPA and DHA through an unidentified pathway. Certain FA components indicate variations in the turnover time when polyps undergo a dietary shift. Understanding the trajectory of FA metabolism across the "phytoplankton-zooplankton" interface, along with its turnover time, provides crucial insights for modeling diet estimation of components within food webs.

Аспекты торгово-экономического сотрудничества России и Таиланда в конце XX – начале XXI вв.

The work examines the modern trade-economic relations between Russia and Thailand in the context of the history of bilateral relations. The period of the 1990–2010s is shown as the next stage of Russian-Thai trade and economic cooperation, which largely maintain continuity, but in the new historical conditions have complete by newfeatures. The author reviews commodity nomenclature and the dynamics of trade turnover over the past 30 years,investigates the factors influencing the volume of Russian-Thai trade, analyzes the level of investment ties and theprofile of Russian and Thai investors.

Effects of Leydig cell elimination on testicular interstitial cell populations: characterization by scRNA-seq and immunocytochemical techniques.

The mammalian testicular interstitial cells are not well-defined. The present study characterized the interstitial cell types and their turnover dynamics in adult rats. Additionally, the heterogeneity of the mesenchymal population and the effects of Leydig cell elimination on interstitial homeostasis were further analyzed by scRNA-seq datasets and immunocytochemical techniques. Interstitial cells were defined at the transcriptomic level by scRNA-seq and then confirmed and quantified with protein markers. The dividing activity of the major cell types was determined by continuous EdU labeling of the animals for one week. Some of the rats were also treated with a dose of ethylenedimethylsulfonate (EDS) to examine how the loss of Leydig cells (LCs) could affect interstitial homeostasis for three weeks. Seven interstitial cell types were identified, including cell types (percentage of the whole interstitial population) as follows: Leydig (44.6%), macrophage and dendritic (19.1%), lymphoid (6.2%), vascular endothelial (7.9%), smooth muscle (10.7%), and mesenchymal (11.5%) cells. The EdU experiment indicated that most cell types were dividing at relatively low levels (<9%) except for the mesenchymal cells (MCs, 17.1%). Further analysis of the transcriptome of MCs revealed 4 subgroups with distinct functions, including 1) glutathione metabolism and xenobiotic detoxification, 2) ROS response and AP-1 signaling, 3) extracellular matrix synthesis and binding, and 4) immune response and regulation. Stem LCs (SLCs) are primarily associated with subgroup 3, expressing ARG1 and GAP43. EDS treatment not only eliminated LCs but also increased subgroup 3 and decreased subgroups 1 and 2 of the mesenchymal population. Moreover, EDS treatment increased the division of immune cells by more than tenfold in one week. Seven interstitial cell types were identified and quantified for rat testis. Many may play more diversified roles than previously realized. The elimination of LCs led to significant changes in MCs and immune cells, indicating the importance of LCs in maintaining testicular interstitial homeostasis.

Isotopic turnover dynamics in larval pacú (Piaractus mesopotamicus): bridging the gap between maternal transmission and trophic ecology

Isotopic turnover dynamics in larval pacú (Piaractus mesopotamicus): bridging the gap between maternal transmission and trophic ecology

Association of type III collagen turnover with cardiovascular outcomes and impact with canagliflozin in the CANVAS Program: A post hoc analysis.

To investigate type III collagen (COL III) turnover in participants from the CANVAS Program biomarker substudy. Biomarkers of COL III formation (PRO-C3) and COL III degradation fragments (C3M and CTX-III) were assessed in baseline and year 3 plasma from patients enrolled in CANVAS, investigating the effect of canagliflozin in participants with type 2 diabetes. The clinical outcomes investigated in this study were hospitalization for heart failure, cardiovascular death and all-cause mortality. Higher levels of PRO-C3 and C3M at baseline were associated with an increased incidence of all investigated outcomes, whereas levels of CTX-III at baseline were not associated with any of the investigated outcomes. Levels of PRO-C3 decreased and levels of CTX-III increased following canagliflozin treatment. An increase from baseline to year 3 in PRO-C3 in the placebo arm was associated with an increased incidence of cardiovascular outcomes, and in all participants was associated with an increased risk of all-cause mortality. The changes in PRO-C3 and CTX-III reflect a shift in the dynamics of COL3 turnover following treatment with canagliflozin. These biomarkers are promising pharmacodynamic tools that can be used to monitor the impact of canagliflozin treatment and possibly other sodium-glucose co-transporter-2 inhibitors on tissue remodelling in future interventional trials.

The Initial Upper Paleolithic from Tolbor-16, Mongolia: Characteristics of the Lithic Assemblage and Their Evolutionary Implications

According to fossil evidence, the Initial Upper Paleolithic (IUP) refers to archaeological assemblages associated with the early dispersal of Homo sapiens across Eurasia. These assemblages are often described as exhibiting a combination of Middle and Upper Paleolithic typo-technological features or, more broadly, as a technology in progression from the former to the latter. Genetic evidence indicates that as members of our species moved across Europe and Asia, they encountered Neanderthal, Denisovan, and possibly other local populations. At the Eurasian scale, the IUP shows considerable variation, but to what extent this corresponds to a relatively unified cultural package strictly associated with our species, or a suite of unrelated technologies reflecting the complex dynamics of a global population turnover, remains unclear. Like most archaeological assemblages, however, the IUP illustrates a combination of adaptive and normative behaviors, along with some stochastic variation. Here, we investigate what drives typo-technological variation within an IUP assemblage to identify traits relevant for larger scale inter-regional comparisons. Specifically, we describe a lithic assemblage dated to ca. 45 ka cal BP from the site of Tolbor-16 in northern Mongolia. We identify three defining aspects of the IUP at the site, namely variation in core shape and size, patterns of blade core reduction, and the presence of Middle Paleolithic typo-technological features. Our goal is to clarify the influence of dynamic reduction processes on assemblage composition and, within the variation observed, to identify typo-technological features that are relevant for inter-regional comparisons and scenarios of population dispersal.

Dual-market quantitative trading: The dynamics of liquidity and turnover in financial markets

Financial market liquidity is a popular research topic. Investor-driven research uses the turnover rate to measure liquidity and generally finds that the higher the stock turnover rate, the lower the returns. However, the traditional financial liquidity theory has been impacted by new machine-driven quantitative trading models. To explore high machine-driven liquidity and the impact of high turnover rates on returns, this study establishes a dual-market quantitative trading system, introduces a variational modal decomposition (VMD)-bidirectional gated recurrent unit (BiGRU) model for data prediction, and uses the back-end Hong Kong foreign exchange market to develop a quantitative trading strategy using the same rotating funds in the U.S. and Chinese stock markets. The experimental results show that given a principal amount of 210,000.00 CNY, the final predicted net return is 226,538.30 CNY, a net return of 107.86%, which is 40.6% higher than the net return of a single Chinese market. We conclude that, under machine-driven trading, increasing liquidity and turnover increase returns. This study provides a new perspective on liquidity theory that is useful for future financial market research and quantitative trading practices.

Understanding stratification and turnover dynamics of a tropical lake using extensive field observations and 3D hydrodynamic simulations

The stratification and turnover dynamics of a tropical lake were evaluated using field observations and 3D hydrodynamic simulations. Located in the Philippines, Sampaloc Lake is a 104-ha and 27-m deep volcanic crater lake with enclosed watershed, which is at risk of the impacts of intensive aquaculture, rapid urbanization and climate change. Temperature, dissolved oxygen (DO) and chlorophyll-a (Chl-a) were measured at seven sampling stations using a multiprobe. Kruskal-Wallis test revealed that the three parameters are not significantly different among stations, indicating that one sampling station can represent the water quality of the whole lake. Schmidt's Stability Index (SSI) and thermocline strength, together with DO and Chl-a gradients decreased from October 2022 (stratified) to January 2023 (turnover). After successfully verifying the 3D numerical model, sensitivity analyses of water temperature to varying weather, together with particle tracking simulations, were implemented to determine the timing of isothermal state, upwelling, partial mixing, and full turnover. Compared to air temperature, variations in wind speed have more pronounced effects on the delay or progression of isothermal conditions in the lake based on SSI, Lake Number and Wedderburn Number. Isothermal conditions do not necessarily coincide with the timing of full turnover, with the latter being delayed by two days than the former, on average. Results revealed that full turnover can occur several weeks earlier with the decrease in AT and increase in WS. This study can advance the understanding of thermal and turnover dynamics of stratified tropical lakes, leading to better management of the water quality of these water bodies.

Multi-genome comparisons reveal gain-and-loss evolution of anti-Mullerian hormone receptor type 2 as a candidate master sex-determining gene in Percidae

BackgroundThe Percidae family comprises many fish species of major importance for aquaculture and fisheries. Based on three new chromosome-scale assemblies in Perca fluviatilis, Perca schrenkii, and Sander vitreus along with additional percid fish reference genomes, we provide an evolutionary and comparative genomic analysis of their sex-determination systems.ResultsWe explored the fate of a duplicated anti-Mullerian hormone receptor type-2 gene (amhr2bY), previously suggested to be the master sex-determining (MSD) gene in P. flavescens. Phylogenetically related and structurally similar amhr2 duplicates (amhr2b) were found in P. schrenkii and Sander lucioperca, potentially dating this duplication event to their last common ancestor around 19–27 Mya. In P. fluviatilis and S. vitreus, this amhr2b duplicate has been likely lost while it was subject to amplification in S. lucioperca. Analyses of the amhr2b locus in P. schrenkii suggest that this duplication could be also male-specific as it is in P. flavescens. In P. fluviatilis, a relatively small (100 kb) non-recombinant sex-determining region (SDR) was characterized on chromosome 18 using population-genomics approaches. This SDR is characterized by many male-specific single-nucleotide variations (SNVs) and no large duplication/insertion event, suggesting that P. fluviatilis has a male heterogametic sex-determination system (XX/XY), generated by allelic diversification. This SDR contains six annotated genes, including three (c18h1orf198, hsdl1, tbc1d32) with higher expression in the testis than in the ovary.ConclusionsTogether, our results provide a new example of the highly dynamic sex chromosome turnover in teleosts and provide new genomic resources for Percidae, including sex-genotyping tools for all three known Perca species.

Phosphate overplus response in Chlamydomonas reinhardtii: Polyphosphate dynamics to monitor phosphate uptake and turnover

There is widespread interest in using microalgae to sustainably control and recover nutrients from wastewater to meet environmental discharge consents and recycle nutrients into agriculture or other applications. Like bacteria and yeasts, microalgae exhibit a phosphate (Pi) overplus response when Pi-deprived cells are resupplied with this nutrient. Quantitative and qualitative methods were used to follow the dynamics of polyP synthesis and turnover in four strains of Chlamydomonas reinhardtii during Pi deprivation followed by nutrient resupply (either as Pi or complete media). The lowest level of in-cell polyP during Pi deprivation, which correlates with the cessation of growth, is the key parameter for timing Pi resupply to maximise the Pi overplus response. Pi deprivation beyond this point leads to a reduced Pi uptake and reduced overplus response. Additional nutrients do not affect the magnitude of the overplus response but are important for continued growth and maximal Pi removal from the media. One strain tested shows enhanced Pi uptake and increased polyP and total in-cell P, suggesting that strain selection is also important. Although polyP levels are maintained after Pi resupply, the polymer is dynamically remodelled. Inositol hexakisphosphate (IP6) increases during this time but does not precede polyP synthesis as predicted by a model where inositol phosphates switch on polyP synthesis. Tracking polyP allows the correct time for nutrient resupply to be determined and therefore a reproducible Pi overplus response to be achieved. Depending on whether maximum cellular P content or maximum Pi removal is desired different strategies may be required. Pi deprivation until growth cessation then resupplying complete nutrients gives the best trade-off between high in-cell P accumulation, high Pi removal and algal biomass growth. This work provides robust measurements of quantitative physiological parameters, which allows reproducibility in laboratory studies and provides design parameters for algal-based nutrient recovery systems from waste waters.

Radiocarbon evidence of organic carbon turnover response to grassland grazing: A soil aggregate fraction perspective

Grassland grazing, driven human activities, represents a prevalent form of land use conversion. While numerous studies have examined the impact of such conversions on soil carbon cycling, they primarily focus on the content and storage of soil organic carbon (SOC). However, research on the turnover dynamics of SOC and the underlying mechanisms triggered by land use conversions remains relatively scarce. In this study, radiocarbon (14C) tracing technology was applied to investigate the effects of grassland grazing on SOC turnover in the Saihanba area of Hebei province, China. The results revealed that the turnover time of SOC in pasture grassland was shortened by approximately 250 years compared to meadow grassland, suggesting that grazing diminishes the ability of topsoil to stabilize SOC. Furthermore, our findings indicate that grazing leads to a decrease in soil CO2 flux by 0.50 g C m−2 y−1 under aggregates larger than 250 μm and those between 63 and 250 μm. Conversely, the CO2 flux under aggregates, specifically those between 2 and 63 μm and less than 2 μm, increased by 0.96 g C m−2 y−1. This shift suggests a significant increase in the contribution of older SOC pools to the overall soil CO2 flux. Our study provides novel insights into SOC cycling in the context of grassland grazing, highlighting the importance of understanding SOC turnover dynamics for effective land management.

Lytic transglycosylase Slt of Pseudomonas aeruginosa as a periplasmic hub protein.

Peptidoglycan is a major constituent of the bacterial cell wall. Its integrity as a polymeric edifice is critical for bacterial survival and, as such, it is a preeminent target for antibiotics. The peptidoglycan is a dynamic crosslinked polymer that undergoes constant biosynthesis and turnover. The soluble lytic transglycosylase (Slt) of Pseudomonas aeruginosa is a periplasmic enzyme involved in this dynamic turnover. Using amber-codon-suppression methodology in live bacteria, we incorporated a fluorescent chromophore into the structure of Slt. Fluorescent microscopy shows that Slt populates the length of the periplasmic space and concentrates at the sites of septation in daughter cells. This concentration persists after separation of the cells. Amber-codon-suppression methodology was also used to incorporate a photoaffinity amino acid for the capture of partner proteins. Mass-spectrometry-based proteomics identified 12 partners for Slt in vivo. These proteomics experiments were complemented with in vitro pulldown analyses. Twenty additional partners were identified. We cloned the genes and purified to homogeneity 22 identified partners. Biophysical characterization confirmed all as bona fide Slt binders. The identities of the protein partners of Slt span disparate periplasmic protein families, inclusive of several proteins known to be present in the divisome. Notable periplasmic partners (KD < 0.5 μM) include PBPs (PBP1a, KD = 0.07 μM; PBP5 = 0.4 μM); other lytic transglycosylases (SltB2, KD = 0.09 μM; RlpA, KD = 0.4 μM); a type VI secretion system effector (Tse5, KD = 0.3 μM); and a regulatory protease for alginate biosynthesis (AlgO, KD < 0.4 μM). In light of the functional breadth of its interactome, Slt is conceptualized as a hub protein within the periplasm.

Isoform and pathway-specific regulation of post-transcriptional RNA processing in human cells.

Steady-state levels of RNA transcripts are controlled by their rates of synthesis and degradation. Here we used nascent RNA Bru-seq and BruChase-seq to profile RNA dynamics across 16 human cell lines as part of ENCODE4 Deeply Profiled Cell Lines collection. We show that RNA turnover dynamics differ widely between transcripts of different genes and between different classes of RNA. Gene set enrichment analysis (GSEA) revealed that transcripts encoding proteins belonging to the same pathway often show similar turnover dynamics. Furthermore, transcript isoforms show distinct dynamics suggesting that RNA turnover is important in regulating mRNA isoform choice. Finally, splicing across newly made transcripts appears to be cooperative with either all or none type splicing. These data sets generated as part of ENCODE4 illustrate the intricate and coordinated regulation of RNA dynamics in controlling gene expression to allow for the precise coordination of cellular functions.

Dynamin-dependent entry of Chlamydia trachomatis is sequentially regulated by the effectors TarP and TmeA

Chlamydia invasion of epithelial cells is a pathogen-driven process involving two functionally distinct effectors – TarP and TmeA. They collaborate to promote robust actin dynamics at sites of entry. Here, we extend studies on the molecular mechanism of invasion by implicating the host GTPase dynamin 2 (Dyn2) in the completion of pathogen uptake. Importantly, Dyn2 function is modulated by TarP and TmeA at the levels of recruitment and activation through oligomerization, respectively. TarP-dependent recruitment requires phosphatidylinositol 3-kinase and the small GTPase Rac1, while TmeA has a post-recruitment role related to Dyn2 oligomerization. This is based on the rescue of invasion duration and efficiency in the absence of TmeA by the Dyn2 oligomer-stabilizing small molecule activator Ryngo 1-23. Notably, Dyn2 also regulated turnover of TarP- and TmeA-associated actin networks, with disrupted Dyn2 function resulting in aberrant turnover dynamics, thus establishing the interdependent functional relationship between Dyn2 and the effectors TarP and TmeA.

Aging decreases osteocyte peri-lacunar-canalicular system turnover in female C57BL/6JN mice

Osteocytes engage in bone resorption and mineralization surrounding their expansive lacunar-canalicular system (LCS) through peri-LCS turnover. However, fundamental questions persist about where, when, and how often osteocytes engage in peri-LCS turnover and how these processes change with aging. Furthermore, whether peri-LCS turnover is associated with natural variation in cortical tissue strain remains unexplored. To address these questions, we utilized confocal scanning microscopy, immunohistochemistry, and scanning electron microscopy to characterize osteocyte peri-LCS turnover in the cortical (mid-diaphysis) and cancellous (metaphysis) regions of femurs from young adult (5 mo) and early-old-age (22 mo) female C57BL/6JN mice. LCS bone mineralization was measured by the presence of perilacunar fluorochrome labels. LCS bone resorption was measured by immunohistochemical marker of bone resorption. The dynamics of peri-LCS turnover were estimated from serial fluorochrome labeling, where each mouse was administered two labels between 2 and 16 days before euthanasia. Osteocyte participation in mineralizing their surroundings is highly abundant in both cortical and cancellous bone of young adult mice but significantly decreases with aging. LCS bone resorption also decreases with aging. Aging has a greater impact on peri-LCS turnover dynamics in cancellous bone than in cortical bone. Lacunae with recent peri-LCS turnover are larger in both age groups. While peri-LCS turnover is associated with variation in tissue strain between cortical quadrants and intracortical location for 22 mo mice, these associations were not seen for 5 mo mice. The impact of aging on decreasing peri-LCS turnover may have significant implications for bone quality and mechanosensation.

ФІНАНСОВИЙ АНАЛІЗ ІННОВАЦІЙНО ОРІЄНТОВАНИХ ПІДПРИЄМСТВ АГРОПРОДОВОЛЬЧИХ СОЦІАЛЬНО-ЕКОНОМІЧНИХ СИСТЕМ В УМОВАХ РИНКОВОГО ЦІНОУТВОРЕННЯ

The following trends characterize the economic development of innovatively oriented enterprises of agri-food socio-economic systems in the conditions of market pricing: there is an increase in the overall production of food products, beverages and tobacco products, in particular pork,poultry and canned goods from them, vegetables, sunflower oil, liquid milk, cereals, non-carbonated mineral water, cigarettes; growth per person. In Ukraine, the production of the following food products and beverages increased: pork and poultry meat, canned vegetables, sunflower oil, liquid milk, butter, and non-carbonated mineral water. At the same time, there was a reduction in the production of sausage products, certain fruit products, margarine, cheese, certain dairy products, flour, bread, flour products, chocolate, confectionery, and beverages. There is a decrease per capita in the production of such food products and beverages in Ukraine as sausages, juices, some other fruit products, margarine, cheese, certain dairy products, bread and bakery products, chocolate, sugar confectionery, carbonated mineral water, beverages non-alcoholic; growth of wholesale turnover of food products and the share of sales of domestic food products with a simultaneous reduction in the specific weight of food in the structure of wholesale turnover. There was a decrease in the volume of retail sales of certain food product groups, in particular, such as: meat and meat products, butter, vegetable oils, cheese, eggs, confectionery, tea, flour, bakery and pasta products, potatoes, vegetables, fruits , berries, grapes; positive dynamics of retail turnover of food products, which is obviously related to the dynamics of solvent demand of the population. We also observe an increase in the specific share of products of vegetable and animal origin, live animals, fats and oil in the structure of the state's exports, while the share of ready-made food products decreases. In general, the volume of food exports increased; the specific weight of ready-made food products, products of plant origin, fats and oils in the structure of national imports increased, while the share of live animals and products of animal origin decreased at the same time