Dynamics Of Changes In Concentration Research Articles

The severity and dynamics of changes in the serum concentration of biochemical markers of muscle tissue damage in an experienced ultramarathon runner after overcoming a distance of 165 km against the background of continuing physical activity

Purpose: to determine the severity and dynamics of changes in the concentration of biochemical markers of muscle tissue damage in the blood serum of an experienced ultramarathon runner after overcoming a distance of 165 kilometers.Materials and methods: measurement of serum concentrations of markers of muscle tissue damage (aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), creatine kinase MB (CK-MB), myoglobin) in serum before and 16 and 144 hours after ultramarathon in an experienced runner (age 36, height 186 cm, weight 76 kg, body mass index 21.96).Results: 16 hours after the end of the race, extremely high levels of AST, ALT, CK, CK-MB and myoglobin were observed, the combination of which indicates severe rhabdomyolysis. After 144 hours, a decrease in the level of all markers of muscle tissue damage, including myoglobin, was noted, however, they continued to remain significantly higher than the reference values. At the same time, the athlete did not have any significant pathological symptoms, and he continued to train in the recovery mode.Conclusion: ultramarathon races lead to significant muscle damage, which can lead to rhabdomyolysis. Its severity may not correlate with any significant clinical manifestations.

Impact of road transport on groundwater quality

Road transport is one of the sources of groundwater pollution. The aim of the article is to find out whether the largest roads in Poland have a negative impact on groundwater quality and to assess the extent of their influence. The water chemistry in the area of roads with heavy traffic in north-west and central Poland in 2007–2020 was analyzed. The groundwater quality status and the dynamics of changes in concentrations of selected chemical constituents were assessed. Data was taken from the national chemical monitoring network managed by the Polish Geological Institute - National Research Institute. The results of monitoring tests at 42 selected points were subject to detailed statistical analysis. The mentioned points were located within 100 m from the roads. A statistical analysis of selected chemical parameters and a correlation matrix for selected components were performed. The distribution of electrolytic conductivity in the analyzed 42 points in 2019 is presented in the form of a contour map against the background of the main roads in the analyzed area. Based on archival data, a 7-year prediction of changes in concentrations of selected chemical components, using the autoregressive method, was made. The predicted distribution of concentrations of individual chemical components in groundwater indicates that the vicinity (up to 50 m) of roads may negatively affect the groundwater quality. The increased concentration of chloride and sodium ions was found in most sites. An upward trend was also observed for specific electrolytic conductivity.

Blood Plasma Proteins Associated With Heart Rate Variability in Cosmonauts Who Have Completed Long-Duration Space Missions.

The study presents the results of evaluating the changes in the concentrations of blood plasma proteins associated with heart rate variability (HRV) in cosmonauts who have completed space missions lasting about 6months. The concentrations of 125 proteins were quantified in biological samples of the cosmonauts’ blood plasma. The subgroups of proteins associated with the physiological processes of the HRV autonomic regulation were identified using bioinformatic resources (Immunoglobulin heavy constant mu, Complement C1q subcomponent subunit C, Plasma serine protease inhibitor, Protein-72kDa type IV collagenase, Fibulin-1, Immunoglobulin lambda constant 3). The concentration of these proteins in the blood plasma before the flight, and the dynamics of concentration changes on the 1st and 7th days of the post-flight rehabilitation period differed in the groups of cosmonauts with a predominance of sympathetic or parasympathetic modulating autonomous influences. The dynamics of changes in the concentrations of the identified set of proteins reveal that in cosmonauts with a predominance of sympathetic modulating influences, the mechanisms of autonomic regulation are exposed to significant stress in the recovery period immediately after the completion of the space mission, compared with the cosmonauts with a predominance of parasympathetic modulating influences.

Assessment of the Effect of A-PRF Application during the Surgical Extraction of Third Molars on Healing and the Concentration of C-Reactive Protein.

Extraction procedures for mandibular third molars are performed all over the world every day. Local inflammation resulting from surgery, and the pain that patients experience, often make it impossible to take up daily life activities, such as work or sports. Growth and anti-inflammatory factors, located in the fibrin network, have a positive effect on tissue-healing processes and should also reduce local inflammation. Advanced platelet-rich fibrin (A-PRF) applied locally influences such processes as: angiogenesis, osteogenesis and collagenogenesis. It also affects mesenchymal cell lines and anti- and pro-inflammatory mediators. Due to the autologous origin of the material, their use in guide bone regeneration (GBR) is more and more widespread in dentistry. The results of previous studies indicate that the use of A-PRF in the treatment area significantly reduces postoperative pain, while the formation of edema is not affected. C-reactive protein (CRP), which is an acute phase protein, appears in the blood as a consequence of inflammation. Due to the dynamics of changes in concentration of CRP, it is a protein that is sufficiently sensitive and is used in studies to monitor the tissue healing process. The effect of A-PRF application on CRP concentrations, before and after surgery, has not been investigated yet. The study was conducted on 60 generally healthy patients. A faster decrease of CRP levels was shown in patients who used A-PRF after the procedure. Additionally, it accelerated healing and reduced the occurrence of a dry socket close to 0.

Dynamics of changes in concentrations of heavy metal group pollutants in surface waters of the Ertis transboundary river on the territory of the Republic of Kazakhstan

Dynamics of changes in concentrations of heavy metal group pollutants in surface waters of the Ertis transboundary river on the territory of the Republic of Kazakhstan

Changes of vitamin E level in adolescent girls with uterine hypoplasia against the background of hypomenstrual syndrome during follow-up study

Objective — to assess changes of vitamin E level in adolescent girls with uterine hypoplasia against the background of hypomenstrual syndrome (HMS) during follow-up study.Materials and methods. We examined 40 adolescent girls, aged 14–18 years, with HMS against the background of uterine hypoplasia, particularly those with oligomenorrhea (OM) and secondary amenorrhea (SA), vitamin E level in whose blood serum we had determined before and after the 2 weeks of treatment. Patients were subjected to background therapy and ultraphonophoresis (UP) of vitamin E.Results and discussion. In the process of follow-up study we identified a correction of tocopherol level in blood serum of patients with HMS: increase in frequency of normal indices and decrease in that of low ones. Moreover, during background therapy this is observed mostly in girls with OM, while during treatment with UP of vitamin E it is evident mostly in those with SA. With background therapy positive dynamics of change in tocopherol level are more frequently registered in girls with ineffective treatment, while in case of using UP of vitamin E they are more often registered in patients with effective treatment.Conclusions. Using non-hormonal methods in treatment of adolescent girls with HMS against the background of uterine hypoplasia leads to positive dynamics of change in concentration of vitamin E, which in turn contributes to emergence of menstruations in every third girl in case of background therapy and in 61% of them in case of UP of vitamin E.

Dynamics of Changes in Concentration of Inflammatory and Destructive Biomarkers at Various Stages of Atherosclerotic Plaque Development

Dynamics of Changes in Concentration of Inflammatory and Destructive Biomarkers at Various Stages of Atherosclerotic Plaque Development

Оцінка клінічного перебігу запальних захворювань тканин пародонта за вмістом середньомолекулярних пептидів у біологічних рідинах в працівників агропромислових виробництв, які працюють в умовах відкритого та закритого ґрунту при впливі пестицидів

The article adduces the dynamics of concentration changes of medium molecular peptides in oral liquid and blood serum in persons with inflammatory diseases of periodontal tissue who work in different conditions of agriculture manufacture at pesticides influence. It was proved that in this contingent the increase of the marker of endogenous intoxication in biological liquids with enlargement of intensification of inflammatory processes in paradontium occurs, moreover, in people who work on closed greenhouse grounds at pesticides influence this tendency has more expressed character than in almost healthy persons of control group in patients with inflammatory diseases of periodontal tissue who don’t suffer from negative influence of harmful factors of agriculture enterprises.

Sustained decrease in plasma sphingosine-1-phosphate concentration and its accumulation in blood cells in acute myocardial infarction

Sphingosine-1-phosphate (S1P) is a cardioprotective sphingolipid present at high concentration in plasma and blood cells. However, effect of the myocardial infarction on S1P metabolism in blood is poorly recognized. Therefore, we aimed to examine the dynamics of changes in concentration of sphingolipids in blood of patients with acute ST-segment elevation myocardial infarction (STEMI). The study was performed on two groups of subjects: healthy controls (n=32) and patients with STEMI (n=32). In the latter group blood was taken upon admission to intensive heart care unit, and then on the second, fifth and thirtieth day, and approximately two years after admission. STEMI patients showed decreased plasma S1P concentration and accumulation of free sphingoid bases and their 1-phosphates in erythrocytes. This effect was already present upon admission, and was maintained for at least thirty days after the infarction. Interestingly, two years post-infarction plasma S1P level recovered only partially, whereas the content of erythrocyte sphingolipids decreased to the values observed in the control subjects. The most likely reason for the observed reduction in plasma S1P level was its decreased release or increased degradation by vascular endothelial cells, as we did not find any evidence for downregulation of S1P synthesis or release by blood cells. We conclude that patients with STEMI are characterized by marked alterations in sphingolipid metabolism in blood which could be a consequence of the infarction itself, the antiplatelet treatment given or both. Our data suggest that cardioprotective action of S1P may be diminished in patients with acute myocardial infarction.

Comparative Study of Selected Blood Biochemical Components in Milk or Milk-Replacer Fed Calves during the Second Week of Life

The experiment was carried out on 13 male Polish Black and White dairy calves of 75% share of the Holstein-Friesian (HF) breed during the second week of life. The animals were divided into two groups. One group (n=7) was fed mother's milk and the second (n=6) milk replacer. The dynamics of changes in concentration of selected blood biochemical components connected with nitrogen metabolism (plasma total protein, albumin, urea, endogenous creatinine) and with mineral metabolism (sodium, chloride, calcium, magnesium, zinc, copper and plasma osmotic pressure) were analyzed in both groups. The results show that the type of ingested food influences the concentration of indicators reflecting nitrogen metabolism. Changes of these parameters in calves fed milk replacer are possibly connected with advantageous catabolic changes. Stable concentrations of main extracellular fluid electrolytes and blood plasma osmotic pressure were found in both groups of calves. Constant blood plasma calcium, magnesium, zinc and copper concentrations observed during this study might also indicate the relative maturity of mechanisms maintaining water and electrolyte balance. Nevertheless, it seemsjustifiable to monitor the copper concentrations in plasma of young calves.