The control performance of a passive dynamic vibration absorber (DVA) is known to be sensitive to its frequency deviation. This study upgrades the conventional device and proposes an adaptive-passive variable frequency gas-spring DVA (APVF-GSDVA) for controlling the structural seismic response, while an adaptive control algorithm is developed that requires only a single input signal. The DVA’s vibration frequency is precisely modulated by adjusting the gas pressure in the gas-spring, aligning it with the inherent frequency of the multi-degree-of-freedom (MDOF) structure. The working principle and control algorithm of the APVF system is introduced and derived in detail, the corresponding gas-spring DVA and implementation are designed, and the configuration of the entire control system is realized. An adaptive variable frequency test scheme is designed and the frequency retuning function of the prototype APVF-GSDVA device installed on an MDOF structure is experimentally validated. The seismic response control effectiveness of the retuned DVA is demonstrated by comparing the detuned DVA, highlighting the necessity of implementing adaptive frequency adjustment of the gas-spring DVA. Experimental findings demonstrate that APVF-GSDVA accurately identifies the inherent frequency of the MDOF structure in ambient excitation, it then adjusts the pressure of the gas-spring according to the frequency-pressure relationship, optimally retuning the DVA. A retuned GSDVA can effectively suppress the structural seismic responses, compared to a detuned DVA, its control effectiveness on peak and RMS responses can be improved by a maximum of nearly 13.5% and 53.3% at a frequency deviation of 10%. Furthermore, results from two “detuning-retuning” test paths with distinct seismic excitations indicate that the retuned DVA exhibits superior control effectiveness, underscoring the significance of the adaptive frequency adjustment function in passive DVAs.