The main function of the surface of a blood cell is to receive information from the environment. Experiments have indicated that the cell membrane plays a vital role in the life, development, and regulation of cells. There is, however, no existing method to quantify the observable changes in membrane shape that occur in locomotion. The main goal of this research is to develop an image interpretation system capable of analyzing the structural changes in the morphology of cells from a sequence of pictures, using automatic techniques of image processing. A model for a general dynamic scene analysis system is described. It consists of three basic entities: dynamic data, static data, and a collection of analysis processes. Based on this model, a rule-based image interpretation system for moving cells has been implemented. The system consists of different cooperating computational processes, which interact with two common memories, a short term memory (STM) and a long term memory (LTM), The STM contains a dynamic record of the instantaneous cell motion, shape, and structural changes, as well as the current global description of the cell behavior. The LTM data arc static, and are implemented as rules. These describe the general model of the morphology of the cells under analysis, as well as control information pertinent to the computational processes. The latter are activated by the control rules throughout the three hierarchical analysis stages: static, incremental, and global. They interact through the STM using the information stored in the LTM, until a complete description of the dynamic cell motion and morphology is obtained. The system has been successfully employed to analyze and study the pseudopod kinetics of white blood cells.
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