Seismic isolation technology plays a crucial role in enhancing earthquake resistance and mitigating disasters for building structures. In this study, the ETABS analysis software V21.0.1 is utilized to establish a numerical model of a six-story steel reinforced concrete frame structure. Both the time-history analysis method and response spectrum method are employed to calculate the seismic response of the model under earthquake actions. The placement of an isolation layer on the foundation and from the first to fifth floor is considered, with separate calculations conducted for each scenario. Subsequently, a comprehensive comparison and analysis of the dynamic response characteristics among different design schemes are performed. The results demonstrate that the most favorable isolation effect is achieved when the isolation layer is implemented on the foundation or first floor. Compared to non-isolated structures, the natural period of the structure can be extended by 2.2 times and 2 times under the base isolation and first-floor top isolation schemes, respectively. The damping coefficients can reach 0.35 and 0.36, respectively, while the inter-story drift angles can be reduced by 66% and 67%, respectively.