During unilateral static and rhythmic handgrip exercise, middle cerebral artery blood velocity (MCAv) increases in the contralateral side to the exercising limb. However, whether this neurovascular coupling-mediated increase in contralateral MCAv is apparent against a background of fluctuating perfusion pressure produced by dynamic resistance exercise (RE) is unclear. We examined the cerebral haemodynamic response to unilateral dynamic RE in 30 healthy individuals (female=16, mean±SD: age, 26±6 years; height, 175±10cm; weight, 74±15kg; body mass index, 24±5kgm-2). Participants completed four sets of 10 paced repetitions (15 repetitionsmin-1) of unilateral bicep curl exercise at 60% of the predicted one-repetition maximum (7±3kg). Beat-to-beat blood pressure, bilateral MCAv and end-tidal carbon dioxide were measured throughout. One-way ANOVA was used to analyse cardiovascular variables and two-way ANOVA to analyse dependent cerebrovascular variables (side×sets, 2×5). A linear mixed model analysis was also performed to investigate the effects of end-tidal carbon dioxide and mean arterial blood pressure on MCAv. In comparison to baseline, within-exercise mean arterial blood pressure increased (P<0.001) across the sets, whereas bilateral MCAv decreased (P<0.001). However, no significant interaction effect was observed for any dependent variables (all P>0.787). The linear mixed model revealed that end-tidal carbon dioxide had the greatest effect on MCAv (estimate=1.019, t=8.490, P<0.001). No differences were seen in contralateral and ipsilateral MCAv during dynamic RE, suggesting that neurovascular coupling contributions during dynamic RE might be masked by other regulators, such as blood pressure.