Buildings are among the primary contributors to global energy consumption, and reducing their demand is one of the challenges that need to be considered for a sustainable future. Zero Energy Buildings (ZEB) represent one of the most promising strategies in this endeavor, and in this sense, the UniZEB project addresses this issue with an innovative approach. It is a Zero Energy Building Laboratory of the University of Padova, built up from a collaboration between local companies, students, and researchers, featuring high performance HVAC and envelope technologies integrated with renewable energy sources. The present work offers an overview of the project, showing some of the already-faced challenges, as well as future opportunities of research and study. The purpose of the laboratory is indeed to offer the students the possibility to put in practice the knowledge they gain through university, as well as exploring new subjects through recent research topics, e.g., the development of a sensor network for the monitoring of the building, the dynamic model calibration, able to compare expected and current energy demand. The paper aims also to demonstrate the potential of a project like UniZEB, proving how research, innovation, and collaboration can shape the future of sustainable construction.