The dynamic deflection measurement is an important component to the bridge loading test of high-speed railway bridge. Using inclinometers to test the Dynamic deflection is a good method for High-Speed Railway bridge deflection detection. In this paper, we develop a new inclinometer and a new theory to fast calculate deflection. It has been use to detect the dynamic deflection of Baishui River bridge on FuZhou-WenZhou High-Speed Railway when high speed train runs across at kinds of speed. Results indicate: comparing with the measurement results of photoelectric deflection detection system, all dynamic deflection measurement results of inclinometers are very accuracy. Their deflection curves are also similar. All results also show: Using our inclinometers to test High-Speed Railway bridge dynamic deflection can get precise results and can satisfy the dynamic deflection measurement request of high-speed railway bridge. Comparing with other methods of dynamic deflection measurement, this method is very easy to operate., do not need stop the traffic, and not affected by weather, and not need a static reference point, can measure the dynamic deflection of any beam bridge.