Dynamic Atomic Force Microscopy Research Articles

Ionic liquid coating for charge mitigation of solar modules in space: Electron microscopy on insulating nanosphere lithography patterned surfaces

Surfaces of satellites and spacecrafts are exposed to high energy charged particles from the solar wind, especially during space weather events. This can lead to differential charging, which is a common reason for hardware degradation and sensor errors. Solutions like coatings are required to avoid excessive cost and weight. Mimicking the electron part of space-like environments in ultra-high vacuum (UHV) chambers can be achieved by using electrons emitted by a scanning electron microscope (SEM). As a performance test for the discharge capabilities we use the quality of electron microscopy images on otherwise insulating substrates such as glass, structured by nanosphere lithography and coated with an ionic liquid (IL). Additionally, the surface potential was measured by Kelvin Probe Force Spectroscopy. The IL film (BMP DCA) was applied ex situ and a thickness of 12.8 ($\pm$0.8)\,nm was determined by reflectometry and confirmed by dynamic atomic force microscopy. Such a film of ionic liquid would lead to additional mass of below 20\,mg and negligible additional material costs. The light absorption and influence of ionic liquid coatings on the current output of an actual solar cell were investigated. The results indicate, that these coatings are promising candidates for surface charge mitigation with a high potential for application

Control of quality factor of atomic force microscopy cantilever by cavity optomechanical effect

The effective quality factor of the cantilever plays a fundamental role in dynamic mode atomic force microscopy. Here we present a technique to modify the quality factor of an atomic force microscopy cantilever within a Fabry–Perot optical interferometer. The experimental setup uses two separate laser sources to detect and excite the oscillation of the cantilever. While the intensity modulation of the excitation laser drives the oscillation of the cantilever, the average intensity can be used to modify the quality factor via optomechanical force without changing the fiber-cantilever cavity length. The technique enables users to optimize the quality factor for different types of measurements without influencing the deflection measurement sensitivity. An unexpected frequency shift was observed and modelled as temperature dependence of the cantilever’s Young’s modulus, which was validated using finite element simulation. The model was used to compensate for the thermal frequency shift. The simulation provided relations between optical power, temperature, and frequency shift.

Enhanced feedback performance in off-resonance AFM modes through pulse train sampling.

Dynamic atomic force microscopy (AFM) modes that operate at frequencies far away from the resonance frequency of the cantilever (off-resonance tapping (ORT) modes) can provide high-resolution imaging of a wide range of sample types, including biological samples, soft polymers, and hard materials. These modes offer precise and stable control of vertical force, as well as reduced lateral force. Simultaneously, they enable mechanical property mapping of the sample. However, ORT modes have an intrinsic drawback: a low scan speed due to the limited ORT rate, generally in the low-kilohertz range. Here, we analyze how the conventional ORT control method limits the topography tracking quality and hence the imaging speed. The closed-loop controller in conventional ORT restricts the sampling rate to the ORT rate and introduces a large closed-loop delay. We present an alternative ORT control method in which the closed-loop controller samples and tracks the vertical force changes during a defined time window of the tip-sample interaction. Through this, we use multiple samples in the proximity of the maximum force for the feedback loop, rather than only one sample at the maximum force instant. This method leads to improved topography tracking at a given ORT rate and therefore enables higher scan rates while refining the mechanical property mapping.

Focused Helium Ion and Electron Beam-Induced Deposition of Organometallic Tips for Dynamic Atomic Force Microscopy of Biomolecules in Liquid.

We demonstrate the fabrication of sharp nanopillars of high aspect ratio onto specialized atomic force microscopy (AFM) microcantilevers and their use for high-speed AFM of DNA and nucleoproteins in liquid. The fabrication technique uses localized charged-particle-induced deposition with either a focused beam of helium ions or electrons in a helium ion microscope (HIM) or scanning electron microscope (SEM). This approach enables customized growth onto delicate substrates with nanometer-scale placement precision and in situ imaging of the final tip structures using the HIM or SEM. Tip radii of <10 nm are obtained and the underlying microcantilever remains intact. Instead of the more commonly used organic precursors employed for bio-AFM applications, we use an organometallic precursor (tungsten hexacarbonyl) resulting in tungsten-containing tips. Transmission electron microscopy reveals a thin layer of carbon on the tips. The interaction of the new tips with biological specimens is therefore likely very similar to that of standard carbonaceous tips, with the added benefit of robustness. A further advantage of the organometallic tips is that compared to carbonaceous tips they better withstand UV-ozone cleaning treatments to remove residual organic contaminants between experiments, which are inevitable during the scanning of soft biomolecules in liquid. Our tips can also be grown onto the blunted tips of previously used cantilevers, thus providing a means to recycle specialized cantilevers and restore their performance to the original manufacturer specifications. Finally, a focused helium ion beam milling technique to reduce the tip radii and thus further improve lateral spatial resolution in the AFM scans is demonstrated.

Impact of long-range attraction on desorption kinetics.

Desorption of molecules from surfaces is widespread both in nature and technology. Despite its omnipresence and conceptual simplicity, fundamental details can be surprisingly complex and are often poorly understood. In many cases, first-order kinetics is assumed, which implies that the adsorbates do not interact with each other and desorption is the rate-limiting process. While this might be a good approximation in some cases, it is far from reality in the case of adsorbates that form ordered structures. Here, we study the desorption of a submonolayer film of 3-nitrophenol from the natural cleavage plane of calcite kept in ultrahigh vacuum. Interestingly, two distinctly different desorption regimes are observed during isothermal desorption monitored by dynamic atomic force microscopy. Initially, at high coverages, the coverage decreases almost linearly in time, indicating a constant desorption rate. Beyond this linear regime, at low coverages, a drastic increase in desorption rate is observed until the surface is completely empty. The transition between these two regimes is associated with a critical island width. We propose an existence of a long-range attractive interaction between the molecules as a possible explanation for the sudden increase in the desorption rate when a critical island width is reached. The herein observed phenomenon of two different desorption regimes is expected to be of general nature when interactions beyond next-neighbour attraction are present.

Analysis of dynamic characteristics of cantilevers excited at the fixed end in dynamic atomic force microscopy in liquid environments

Analysis of dynamic characteristics of cantilevers excited at the fixed end in dynamic atomic force microscopy in liquid environments

Self‐Healing Polybutadienes Formed through Sigmatropic Rearrangement and Stratified H‐Bonding for Use in Polymer‐Bonded Explosives

AbstractThe accomplishment of self‐healing polybutadienes (PB) is decisive for multitude of aerospace applications. We here present the first‐time report on a self‐healing PB derived from a blend of allyloxy end functionalized PB elicited through intramolecular thermally activated sigmatropic Claisen rearrangement coupled with stratified hydrogen bonding for use in polymer bonded explosives (PBX). Heteronuclear Single Quantum Correlation (HSQC), Heteronuclear Multiple Bond Coherence (HMBC), Total Correlation Spectroscopy (TOCSY) and Fourier Transform Infrared spectroscopy (FTIR) Spectroscopy were utilized to confirm the mechanistic aspects of the rearrangement for the proposed application. The mechanism of self‐healing is further elucidated using density functional theory (DFT) computations and confirmed through Raman spectroscopy. The biphasic polymer network architecture is disclosed through two glass transitions at −54 °C and 15–25 °C, as confirmed by Dynamic Mechanical Analysis (DMA) and Atomic Force Microscopy (AFM). The polymer exhibited healing efficiency of 85 % suitable for adhesive or coating applications. Self‐healing in bonding formulations of insensitive polymer bonded explosives was also expedited which exhibited a healing efficiency of 49–54 %.

Rapid method for detection of Vibrio cholerae from drinking water with nanomaterials enhancing electrochemical biosensor

Cholera is a potentially fatal diarrheal disease caused by Vibrio cholerae and is spread to humans from contaminated food and water. In order to prevent spread of epidemic chlorea, the development of novel sensitive, selective, user-friendly, cost-effective and rapid detection systems to detect of V. cholerae are necessary. Therefore, in this study, it was aimed to develop a specific, electrochemical immunoassay with high selectivitiy and sensitivity for detection of V. cholerae from drinking water using in house synthesized Gold Nanoparticles (AuNPs). The synthesized AuNPs were characterized by UV/Vis spectroscopy, Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM) and electrochemical techniques were applied to confirm the succesful fabrication of the immunosensor. Also, this study focuses on the development of an antibody sensor for V. cholerae detection using a standard immunoassay without using nanoparticle. To accomplish that, in house spherical synthesized AuNPs at various sizes were synthesized, conjugated with secondary antibody-horseradish peroxidase enzyme (HRP) complex and their possible effect on the lowest detection limit of V. cholerae was investigated in comparison to commercially available AuNPs. The AuNPs-immunosensor on the results enabled the quantification of V. cholerae in a wide concentration range with a high sensitivity limit of detection (1 Colony-Forming Units/Milliliter) and specificity. Although the effect of 33 and 54 nm AuNPs on the process is close to each other, it has been observed that there is a 34% loss of efficiency when the size of the nanoparticle increases. With this study, a novel V. cholerae specific immunosensor was developed and the effects of in house synthesized AuNPs with various diameter on this developed biosensor were investigated in detail.

Chemo-mechanical forces modulate the topology dynamics of mesoscale DNA assemblies

The intrinsic complexity of many mesoscale (10–100 nm) cellular machineries makes it challenging to elucidate their topological arrangement and transition dynamics. Here, we exploit DNA origami nanospring as a model system to demonstrate that tens of piconewton linear force can modulate higher-order conformation dynamics of mesoscale molecular assemblies. By switching between two chemical structures (i.e., duplex and tetraplex DNA) in the junctions of adjacent origami modules, the corresponding stretching or compressing chemo-mechanical stress reversibly flips the backbone orientations of the DNA nanosprings. Both coarse-grained molecular dynamics simulations and atomic force microscopy measurements reveal that such a backbone conformational switch does not alter the right-handed chirality of the nanospring helix. This result suggests that mesoscale helical handedness may be governed by the torque, rather than the achiral orientation, of nanospring backbones. It offers a topology-based caging/uncaging concept to present chemicals in response to environmental cues in solution.

Poly(glycidyl methacrylate) modified cellulose nanocrystals and their PBAT-based nanocomposites

Melt processing of cellulose nanocrystals (CNCs) reinforced nanocomposites is still a serious challenge due to the hydrophilic nature of CNCs and their severe agglomeration tendency within the polymer melt. In this study, chemical modification of CNC through grafting poly(glycidyl methacrylate) (PGMA) with various degrees was implemented. Wettability of the modified CNCs (mCNCs) were controlled and their structure was characterized through Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), optical microscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The nanocomposites of polybutylene adipate terephthalate (PBAT) with 3 wt% CNC and mCNC were prepared using an internal melt mixer. To differentiate the effects of CNC and PGMA molecules on the final properties of nanocomposites, PBAT/PGMA compounds were separately prepared. To confirm the chain characterization and molecular weight of the synthesized PGMAs, 1H NMR and gel permeation chromatography (GPC) analysis were conducted. Melt rheological analysis, dynamic mechanical analysis (DMA), DSC, and atomic force microscopy (AFM) were used to monitor the mCNC dispersion quality and the effect of PGMA modification in PBAT compounds. The results revealed that grafting CNC with longer PGMA considerably improved the CNCs' dispersion quality within PBAT. Such dispersion enhancement of long-chain mCNCs and interfacial interaction of PGMA and PBAT resulted in a noticeable increase in storage modulus and complex viscosity of the final nanocomposites.

Microstructural characteristics evolution of asphalt mastics under cyclic environmental temperature variations

Environmental temperature-effect is the primary factor responsible for asphalt pavement failure. Asphalt pavement exposed to the atmosphere is constantly subjected to the thermal cycles induced by environmental temperature fluctuations, and the resulting thermal cracks significantly reduce the durability of asphalt pavement. Nevertheless, the influence of cyclic temperature variations on the performance degradation of asphalt binder materials is often overlooked. To address this, accelerated thermal cycling test was conducted on two asphalt mastics to simulate repeated environmental temperature variations. Dynamic shear rheological test (DSR) and atomic force microscopy test (AFM) were performed to characterize the evolution of the macroscopic rheological properties and microstructural characteristics under five cyclic temperature ranges. Pearson correlation analysis was utilized to explore the correlation between macro and micro properties. Following thermal cycling, it was observed that the two asphalt mastics experienced a decline in their viscous properties and became more elastic, resulting in a decrease in their stress relaxation capacity and asphalt-aggregate interaction on a macroscopic level. The microstructure transitioned from a multiphase to a homogeneous structure, while the expansion of the valley structure indicated the accumulation of microscopic defects. The larger the temperature variations and the higher the cyclic temperatures are, the more severe the deterioration in the viscoelasticity and microstructures of asphalt mastics is; the degradation of the micromechanical characteristics directly affects the macroscopic rheological properties. The bee structure ratio, the adhesion, and relaxation properties are sensitive to cyclic temperature variations and recommended to characterize the thermal cycling resistance. In comparison to 90# asphalt mastic, the SBS modified asphalt mastic exhibits great resistance to thermal deterioration. This study deepens the understanding of the causes of asphalt pavement performance deterioration in regions with frequent environmental temperature fluctuations.

Dielectric constant measurement sensitivity in electrostatic force and force gradient microscopy-based modes

Understanding the nanoscale electrostatic interaction between a conductive atomic force microscopy (AFM) probe and a dielectric film is central to the operation of various nanoscale dielectric microscopies and determination of dielectric properties of the film. There is no simple analytical description of the electrostatic interaction generated in the confined probe-sample geometry for neither static nor dynamic AFM modes used in dielectric measurements. An accurate description of the involved physics is obtained only by means of a finite element analysis modeling of the system. However, the alternative to using the numerical analysis is not very popular due to being slower and requiring relatively high computation resources. In this work, we revised the contributions from different parts of the AFM probe to the probe-sample capacitance by both analytical and numerical methods. We tried to reconciliate the two approaches and observed the differences as a function of geometry and material parameters. Under various noise levels, the efficiency of an analytical model was tested against a finite element analysis that captures in detail the electrostatic interaction in AFM-based dielectric measurements. The investigation was performed in both spectroscopic force-distance curves and constant height scans with measurements for deflection and frequency of the AFM probe. The obtained measurement sensitivities are relevant in selecting the optimal scanning mode and its operational parameters for a given film thicknesses and dielectric constants but also show the critical role of the numerical analysis to the correct interpretation of the measurements.

Direct-current output of silicon–organic monolayer–platinum Schottky TENGs: Elusive friction-output relationship

Triboelectric nanogenerators (TENGs) are an emerging energy harvesting technology able to convert ubiquitous mechanical energy into electricity. Friction, static charging and flexoelectricity are all involved in the mechanism underpinning TENG operation, but their relative contribution has remained elusive. Here we used dynamic and static conductive atomic force microscopy (C-AFM) measurements on monolayer-modified silicon crystals to detect evidence of a relationship between friction and zero-bias current, and between pressure and the direction of the putative flexovoltage. We demonstrate that a static electricity-related tribovoltage is probably responsible for a friction excess, and that surprisingly this friction excess is found to be dependent on the doping level and type of the silicon substrate. Such friction excess is however no longer measurable once current is allowed to flow across the junction. This observation points to an electrostatic origin of friction in silicon-based Schottky TENGs, and suggests that the zero external bias DC current is at least in part an electronic flow to neutralize static charges. Further, the sign of the zero-bias current, but not its magnitude, is independent of the semiconductor doping type, which is again suggestive of surface statics being a main contributor to the zero-bias output rather than exclusively a space-charge effect. We also reveal the presence of a junction flexovoltage under pressures common in AFM experiments (GPa), even for negligible lateral friction. In a static Pt–monolayer–n-type Si junction the flexovoltage carries the same sign as the tribovoltage, and can reach such magnitude to overwrite external voltages as high as 2 V. The immediate implication is that the flexovoltage is likely to have i) a strong contribution to the zero-bias output of a n-Si Schottky TENG, ii) a negative effect on the output of a p-Si TENG, and iii) its detection can be straightforward, as we discovered that flexoelectricity manifests as an “inverted diode”: a n-type Si–platinum diode with negligible current even when the n-type material is negatively biased as long as the “static” diode remains under a large normal pressure.

Molecular Dynamics of Drying-Induced Structural Transformations in a Single Nanocellulose.

Nanocellulose is attracting attention in the field of materials science as a sustainable building block. Nanocellulose-based materials, such as films, membranes, and foams, are fabricated by drying colloidal dispersions. However, little is known about how the structure of a single nanocellulose changes during the complex drying process. Here, all-atom molecular dynamics simulations and atomic force microscopy is used to investigate the structural dynamics of single nanocellulose during drying. It is found that the twist morphology of the nanocellulose became localized along the fibril axis during the final stage of the drying process. Moreover, it is shown that conformational changes at C6 hydroxymethyl groups and glycoside bond is accompanied by the twist localization, indicating that the increase in the crystallinity occurred in the process. It is expected that the results will provide molecular insights into nanocellulose structures in material processing, which is helpful for the design of materials with advanced functionalities.

Computational and Experimental Determination of the Properties, Structure, and Stability of Peptoid Nanosheets and Nanotubes.

Peptoids (N-substituted glycines) are a group of highly controllable peptidomimetic polymers. Amphiphilic diblock peptoids have been engineered to assemble crystalline nanospheres, nanofibrils, nanosheets, and nanotubes with biochemical, biomedical, and bioengineering applications. The mechanical properties of peptoid nanoaggregates and their relationship to the emergent self-assembled morphologies have been relatively unexplored and are critical for the rational design of peptoid nanomaterials. In this work, we consider a family of amphiphilic diblock peptoids consisting of a prototypical tube-former (Nbrpm6Nc6, a NH2-capped hydrophobic block of six N-((4-bromophenyl)methyl)glycine residues conjugated to a polar NH3(CH2)5CO tail), a prototypical sheet-former (Nbrpe6Nc6, where the hydrophobic block comprises six N-((4-bromophenyl)ethyl)glycine residues), and an intermediate sequence that forms mixed structures ((NbrpeNbrpm)3Nc6). We combine all-atom molecular dynamics simulations and atomic force microscopy to determine the mechanical properties of the self-assembled 2D crystalline nanosheets and relate these properties to the observed self-assembled morphologies. We find good agreement between our computational predictions and experimental measurements of Young's modulus of crystalline nanosheets. A computational analysis of the bending modulus along the two axes of the planar crystalline nanosheets reveals bending to be more favorable along the axis in which the peptoids stack by interdigitation of the side chains compared to that in which they form columnar crystals with π-stacked side chains. We construct molecular models of nanotubes of the Nbrpm6Nc6 tube-forming peptoid and predict a stability optimum in good agreement with experimental measurements. A theoretical model of nanotube stability suggests that this optimum is a free energy minimum corresponding to a "Goldilocks" tube radius at which capillary wave fluctuations in the tube wall are minimized.

Well-Defined Shell-Sheddable Core-Crosslinked Micelles with pH and Oxidation Dual-Response for On-Demand Drug Delivery.

Micellar-nanocarrier-based drug delivery systems possessing characteristics such as an excellent circulation stability, inhibited premature release and on-demand site-specific release are urgently needed for enhanced therapeutic efficacy. Therefore, a novel kind of shell-sheddable core-crosslinked polymeric micelles with pH and oxidation dual-triggered on-demand drug release behavior was facilely constructed. The multifunctional micelles were self-assembled from a carefully designed amphiphilic triblock PEGylated polyurethane (PEG-acetal-PUBr-acetal-PEG) employing an acid-labile acetal linker at the hydrophilic-hydrophobic interface and pendant reactive bromo-containing polyurethane (PU) as the hydrophobic block, followed by a post-crosslinking via oxidation-cleavable diselenide linkages. These well-defined micelles exhibited an enhanced structural stability against dilution, achieved through the incorporation of diselenide crosslinkers. As expected, they were found to possess dual pH- and oxidation-responsive dissociation behaviors when exposure to acid pH (~5.0) and 50 mM H2O2 conditions, as evidenced using dynamic light-scattering (DLS) and atomic force microscopy (AFM) analyses. An in vitro drug release investigation showed that the drug indomethacin (IND) could be efficiently encapsulated in the micelles, which demonstrated an inhibited premature release compared to the non-crosslinked ones. It is noteworthy that the resulting micelles could efficiently release entrapped drugs at a fast rate in response to either pH or oxidation stimuli. Moreover, the release could be significantly accelerated in the presence of both acid pH and oxidation conditions, relative to a single stimulus, owing to the synergetic degradation of micelles through pH-induced dePEGylation and oxidation-triggered decrosslinking processes. The proposed shell-sheddable core-crosslinked micelles with a pH and oxidation dual-response could be potential candidates as drug carriers for on-demand drug delivery.

Revealing the static and dynamic nanomechanical properties of diatom frustules—Nature's glass lace

Diatoms are single cell microalgae enclosed in silica exoskeletons (frustules) that provide inspiration for advanced hybrid nanostructure designs mimicking multi-scale porosity to achieve outstanding mechanical and optical properties. Interrogating the structure and properties of diatoms down to nanometer scale leads to breakthrough advances reported here in the nanomechanical characterization of Coscinodiscus oculus-iridis diatom pure silica frustules, as well as of air-dried and wet cells with organic content. Static and dynamic mode Atomic Force Microscopy (AFM) and in-SEM nanoindentation revealed the peculiarities of diatom response with separate contributions from material nanoscale behavior and membrane deformation of the entire valve. Significant differences in the nanomechanical properties of the different frustule layers were observed. Furthermore, the deformation response depends strongly on silica hydration and on the support from the internal organic content. The cyclic loading revealed that the average compliance of the silica frustule is 0.019 m/N and increases with increasing number of cycles. The structure–mechanical properties relationship has a direct impact on the vibrational properties of the frustule as a complex micrometer-sized mechanical system. Lessons from Nature’s nanostructuring of diatoms open up pathways to new generations of nano- and microdevices for electronic, electromechanical, photonic, liquid, energy storage, and other applications.

Biosynthesis, Characterization and Antioxidant Properties of ZnO Nanoparticles Using Punica Granatum Peel Extract as Reducing Agent

The green synthesis method of nanoparticles using plant extracts attracts great attention as a reliable, low-cost, sustainable, environmentally friendly protocol that prevents or minimizes waste generation. In this study, Punica granatum peel extract was used as the reducing plant material and zinc acetate dihydrate (Zn (CH3COO)2. 2H2O) solution was used as the starting metal. In the synthesis, optimum conditions were determined by UV visible spectroscopy using different metal ion concentrations, plant extract amount, temperature, and pH parameters. For characterization of ZnONPs synthesized at optimum conditions, Scanning Electron Microscopy (SEM), Energy Dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), Dynamic Light Scattering (DLS), Zeta potential and Atomic Force Microscope (AFM) analyzes were made. It has been determined that the synthesized ZnONPs are spherical, have good stability, high purity, and nanoscale. The free radical scavenging capacity of biosynthesized ZnONPs was evaluated by DPPH analysis with different concentrations. The IC50 value was determined as 250µg ml-1.

Simulations of Subnanometer Scale Image Contrast in Atomic Force Microscopy of Self-Assembled Monolayers in Water.

Achieving high-resolution images using dynamic atomic force microscopy (AFM) requires understanding how chemical and structural features of the surface affect image contrast. This understanding is particularly challenging when imaging samples in water. An initial step is to determine how well-characterized surface features interact with the AFM tip in wet environments. Here, we use molecular dynamics simulations of a model AFM tip apex oscillating in water above self-assembled monolayers (SAMs) with different chain lengths and functional groups. The amplitude response of the tip is characterized across a range of vertical distances and amplitude set points. Then relative image contrast is quantified as the difference of the amplitude response of the tip when it is positioned directly above a SAM functional group vs positioned between two functional groups. Differences in contrast between SAMs with different lengths and functional groups are explained in terms of the vertical deflection of the SAMs due to interactions with the tip and water during dynamic imaging. The knowledge gained from simulations of these simple model systems may ultimately be used to guide selection of imaging parameters for more complex surfaces.

Co-Assembly of Carbon Nanotube Porins into Biomimetic Peptoid Membranes.

Robust and cost-effective membrane-based separations are essential to solving many global crises, such as the lack of clean water. Even though the current polymer-based membranes are widely used for separations, their performance and precision can be enhanced by using a biomimetic membrane architecture that consists of highly permeable and selective channels embedded in a universal membrane matrix. Researchers have shown that artificial water and ion channels, such as carbon nanotube porins (CNTPs), embedded in lipid membranes can deliver strong separation performance. However, their applications are limited by the relative fragility and low stability of the lipid matrix. In this work,wedemonstrate that CNTPs can co-assemble into twodimension(2D) peptoid membrane nanosheets, opening up a way to produce highly programmable synthetic membranes with superior crystallinity and robustness. A combination of molecular dynamics (MD) simulations, Ramanspectroscopy, X-ray diffraction(XRD), and atomicforcemicroscopy(AFM) measurements to verify the co-assembly of CNTP and peptoids are used and show that it does not disrupt peptoid monomer packing within the membrane. These results provide a new option for designing affordable artificial membranes and highly robust nanoporous solids.