Dynamic Acylhydrazone Research Articles

Anti-inflammatory dressing based on hyaluronic acid and hydroxyethyl starch for wound healing

Eliminating persistent inflammation and choosing dressings that provide the best healing environment is key to promoting wound healing. Dynamic and reversible hydrogels have attracted much attention because of their capacity to adapt to irregular wound surfaces. Herein, oxidized hydroxyethyl starch (OHES) and hyaluronic acid (HA-ADH) were crosslinked via the dynamic acylhydrazone bond to form an anti-inflammatory function hydrogel (HA-ADH/OHES@XT) that could release xanthatin (XT) slowly. The HA-ADH/OHES hydrogels showed an appropriate gelation time, notable water-retaining capacity, self-healing, suitable biodegradability, and good biocompatibility for wound healing applications. In vivo experiments demonstrated that HA-ADH/OHES@XT hydrogels promoted tissue regeneration and wound healing at a rate of approximately 89.1 % on day 20 by reducing inflammation, increasing collagen deposition, and promoting re-epithelialization, indicating their great potential as a wound dressing.

A hyaluronic acid hydrogel as a mild photothermal antibacterial, antioxidant, and nitric oxide release platform for diabetic wound healing

Hyaluronic acid (HA)-based biopolymer hydrogels are promising therapeutic dressings for various wounds but still underperform in treating diabetic wounds. These wounds are extremely difficult to heal and undergo a prolonged and severe inflammatory process due to bacterial infection, overexpression of reactive oxygen species (ROS), and insufficient synthesis of NO. In this study, a dynamic crosslinked hyaluronic acid (HA) hydrogel dressing (Gel-HAB) loaded with allomelanin (AMNP)-N, N′-dis-sec-butyl-N, N′-dinitroso-1, 4-phenylenediamine (BNN6) nanoparticles (AMNP-BNN6) was developed for healing diabetic wounds. The dynamic acylhydrazone bond formed between hydrazide-modified HA (HA-ADH) and oxidized HA (OHA) makes the hydrogel injectable, self-healing, and biocompatible. The hydrogel, loaded with AMNP-BNN6 nanoparticles, exhibits promising ROS scavenging ability and on-demand release of nitric oxide (NO) under near-infrared (NIR) laser irradiation to achieve mild photothermal antibacterial therapy (PTAT) (∼ 48 °C). Notably, the Gel-HAB hydrogel effectively reduced the oxidative stress level, controlled infections, accelerated vascular regeneration, and promoted angiogenesis, thereby achieving rapid healing of diabetic wounds. The injectable self-healing nanocomposite hydrogel could serve as a mild photothermal-enhanced antibacterial, antioxidant, and nitric oxide release platform for the treatment of diabetic wounds.

Preparation and properties of self-healing SBS modified bitumen with dynamic acylhydrazone bonds

Styrene-butadiene-styrene triblock copolymer (SBS) modified bitumen (SMB) with excellent self-healing performance not only saves resources, but is also important for the sustainable development of pavement and construction industry. In this paper, a self-healing agent (SHA) with acylhydrazone bond was synthesized, and the dynamic acylhydrazone bond was introduced into the SBS molecular chains to prepare the self-healing SMB (SHMB). The structure of SHA was characterized, and the physical properties, chemical structure, microstructure and self-healing performance of the SHMB were investigated. Fourier transform infrared (FTIR) spectra and nuclear magnetic resonance spectra demonstrated that SHA was successfully synthesized. The physical performances of SMB were significantly improved by SHA and its optimal dosage was 0.6 %. FTIR indicated that the dynamic acylhydrazone bond was introduced into the SBS molecular chains, and formed hydrogen bonds in SHMB. The fluorescence microscope showed that SHMB had a denser network structure than SMB. The crack healing observation indicated that the crack healing speed of SHMB was much higher than that of SMB. The break tensile performance recovery showed that the recovery rate of elongation at break (Rε) of SMB and SHMB were 14.38 % and 58.27 % after self-healing at 25℃ for 32 h, and the Rε of SMB and SHMB was elevated to 50.12 % and 92.58 % after self-healing at 40℃ for 4 h, which indicated that the introduction of dynamic acylhydrazone bonds greatly enhanced the self-healing ability of SHMB.

Multifunctional On-Demand Removability Hydrogel Dressing Based on in Situ Formed AgNPs, Silk Microfibers and Hydrazide Hyaluronic Acid for Burn Wound Healing.

Elevated temperatures can deactivate tissues in the burn wound area, allowing pathogenic bacteria to multiply on the wound surface, ultimately leading to local or systemic infection. An ideal burn dressing should provide antibacterial properties and facilitate painless dressing changes. Silk microfibers coated with poly (2, 3, 4-trihydroxybenzaldehyde)(referred to as mSF@PTHB) to in situ reduce AgNO3 to silver nanoparticles (AgNPs) in a hydrazide hyaluronic acid-based hydrogel are utilized. The findings indicate a more homogeneous distribution of the silver elements compared to directly doped AgNPs, which also conferred antioxidant and antibacterial properties to the hydrogel. Moreover, hydrogels containing pH-responsive dynamic acylhydrazone bonds can undergo a gel-sol transition in a weak acid environment, leading to the painless removal of adhesive hydrogel dressings. Notably, the on-demand replaceable self-healing antioxidant hydrogel dressing exhibits antibacterial effects and cytocompatibility in vitro, and the wound-healing performance of the hydrogel is validated by treating a burn mouse model with full-thickness skin defects. It is demonstrated that hydrogel dressings offer a viable therapeutic approach to prevent infection and facilitate the healing of burn wounds.

Micro-environment triple-responsive hyaluronic acid hydrogel dressings to promote antibacterial activity, collagen deposition, and angiogenesis for diabetic wound healing.

The clinical treatment of chronic diabetic wounds is a long-standing thorny issue. Strategies targeting the diabetic micro-environment have been developed to promote wound healing. However, it remains challenging to reverse the adverse conditions and re-activate tissue regeneration and angiogenesis. In this work, we develop injectable hydrogels that are responsive to acidic conditions, reactive oxygen species (ROS), and high glucose levels in a diabetic wound micro-environment to sustainably deliver tannic acid (TA) to augment antibacterial, anti-inflammatory, and anti-oxidative activities. This triple-responsive mechanism is designed by introducing dynamic acylhydrazone and phenylboronic ester bonds to crosslink modified hyaluronic acid (HA) chains. At a diabetic wound, the acylhydrazone bonds may be hydrolyzed at low pH. Meanwhile, glucose may compete with TA, and ROS may oxidize the C-B bond to release TA. Thus, sustained release of TA is triggered by the diabetic micro-environment. The released TA effectively scavenges ROS and kills bacteria. In vivo experiments on diabetic mice demonstrate that the hydrogel dressing highly promotes angiogenesis and extracellular matrix (ECM) deposition, leading to eventual full healing of diabetic skin wounds. This micro-environment-triggered triple-responsive drug release provides a promising method for chronic diabetic wound healing.

Dynamic covalent hydrogel fabricated in porous media through dynamic acylhydrazone bond from reactive precursors for deep profile control

In this work, we proposed a novel hydrogel system for in-depth conformance control through the dynamic covalent bond. Diffluent ketone-bearing and acyl hydrazide-bearing reactive precursors with low molecular weight are injected into the formation simultaneously, then gels in situ through dynamic hydrazone bonds to divert the post-injected fluid to un-swept oil zones. Ketone-bearing and acyl hydrazide-bearing reactive precursors with different reactive group ratios were synthesized by simple radical polymerization and hydrazinolysis. Rheological measurement results proved the two reactive precursors formed dynamic covalent hydrogel (DCG) through dynamic hydrazone bonds in deionized water and mineralized water. The viscoelasticity, flow behavior, and profile control behavior in the porous media of the DCG were evaluated. When the flow behavior and profile control experiments were conducted with a 9 m core, the injection pressure of DCG-20 % of which the molar ratio of the reactive group was 20 % was low (0–0.22 MPa) throughout the injection process, and the resistance factor was 4.40. After fully gelation, the post-water injection pressure significantly increased (0.22–7.54 MPa). The residual resistance factor was 150.80, and the plugging efficiency was 99.34 %. This work proved that the DCG consisting of ketone-bearing and acyl hydrazide-bearing reactive precursors not only had a strong injection capacity but also had a good profile control effect in porous media which meant the DCG fully met the need for deep profile control.

High‐performance vitrimer films containing acylhydrazone dynamic covalent bonds

AbstractVitrimer films containing acylhydrazone bonds are synthesized in two steps. Bio‐based vanillin and adipate dihydrazide are used to prepare diols containing acylhydrazone bonds in one step, which are introduced into the reaction system of vitrimer to obtain the final films. The process is concise and easy to operate. By utilizing the rigid structure of vitrimer films and introducing dynamic acylhydrazone bonds, the obtained vitrimer films exhibit strong mechanical strength (maximum tensile strength of 27.10 MPa and maintaining up to 185.52% of the original breaking strength), excellent self‐healing property (tensile strength of 26.26 MPa after 12 h of primary self‐healing and 5.24 MPa after 12 h of secondary self‐healing), and rapid shape memory property (recovery time of about 2 min), strong acid and alkali resistance (not dissolved within 24 h), good thermal stability (no decomposition occurs below 160 °C), and high storage modulus (reaches 1087 MPa). These comprehensive benefits yield a certain reference for the preparation of vitrimer materials with prime properties.

Dynamic acylhydrazone bonds cross-linked chromotropic photonic-ionic skin with self-healing and high resilience for interactive human-machine interface

Advanced skin systems realize perception and interaction with the environment through synergistic electrical and color signals generated by transmitting ions and manipulating photonic structures. Here, inspired by chameleon skins, a novel self-healing and resilient photonic-ionic skin (PI-skin) with electro-optical dual response is ingeniously constructed integrating the ordered micro-nano array and the dynamically covalently cross-linked ionogel. The elaborately designed polycation network with weak inter-chain interactions can promote the uniform distribution of high-content low-viscosity ionic liquid (IL), effectively eliminating energy dissipation and resulting in high resilience. Dynamic acylhydrazone bonds are served as reversible chemical crosslinking to realize high-efficiency self-healing and further enhance resilience. Notably, the artificially manipulated transition of the photonic array from dense packing to non-dense packing endows sensitive chromotropic properties synchronized with electrical response under stretched (Δλ > 130 nm) and compressed (Δλ > 160 nm). Furthermore, by harnessing the correspondence between electrical and optical signals, the control of optical signals over electrical signals is achieved for inputting complex coded information and constructing interactive human–machine interfaces. This work not only inspires the design and construction of high-level artificial skins but also stimulates the development of visual interactive devices and smart electronic/ionic products.

Highly stretchable, fast self-healing nanocellulose hydrogel combining borate ester bonds and acylhydrazone bonds

Self-healing hydrogels have received considerable attention as a promising material for flexible electronic devices given their mechanical durability and structurally tunable properties. In this study, a highly stretchable self-healing hydrogel with dual cross-linking network was developed via borate ester bonds generated by polyvinyl alcohol and borax, and acylhydrazone bonds formed by aldehyde nanocellulose with adipic acid dihydrazide-modified alginate. Compared with the single network hydrogel composed of polyvinyl alcohol and borax, the introduction of dynamic acylhydrazone bonds greatly increases the flexibility of the hydrogel. The elongation rate increased from 480 % to approximately 1440 %, and the self-healing efficiency increased from 84.6 % to 92.7 % after healing for 60 min at ambient temperature without any stimulus. Moreover, the longer the self-healing time, the more evident the self-healing effect of the acylhydrazone bonds. In addition, electrical measurements confirmed a wide working strain range (ca.1000 %), durability, and reliability. Once assembled as a strain sensor, the hydrogel is able to monitor both large and subtle human motions. Besides, this hydrogel exhibited desirable biocompatibility, as demonstrated by in vitro cytotoxicity towards NIH 3T3 cells. These integrated properties make this nanocomposite hydrogel a promising candidate for future applications as green, flexible, and smart sensors.

Dual-responsive bioconjugates bearing a bifunctional adaptor for robust cytosolic peptide delivery.

Peptide drugs have been successfully used for the treatment of various diseases. However, it is still challenging to develop therapeutic peptides working on intracellular targets due to their poor membrane permeability. Here, we proposed a type of dual-responsive bioconjugates bearing a heterobifunctional adaptor containing both aldehyde and catechol moieties for efficient cytosolic peptide delivery. Hydrazine-terminated cargo peptides were tagged to a boronated dendrimer with the help of the adaptor via dynamic acylhydrazone and catechol‑boronate linkages. The bioconjugates efficiently delivered peptides with distinct physicochemical properties into various cells, and could release the cargo peptides triggered by intracellular reactive oxygen species and endolysosomal acidity, restoring the biofunctions of delivered peptides. In addition, the designed complexes efficiently delivered a pro-apoptotic peptide into osteosarcoma cancer cells and successfully inhibited the tumor growth both in vitro and in vivo. This study provides a universal and efficient platform for cytosolic therapeutic peptide delivery to intracellular targets for treating various diseases.

Fabrication of thermo and pH-dual sensitive hydrogels with optimized physiochemical properties via host-guest interactions and acylhydrazone dynamic bonding

Stimuli-responsive polymeric hydrogels based on host-guest interactions have demonstrated potential as smart drug delivery platforms. However, host-guest interactions alone have been repeatedly highlighted to provide insufficient mechanical strength for the formed hydrogels likely due to the generally low grafting degrees of functional groups and steric hindrance of polymer backbone and grafted functions. Herein, a combination strategy of reversible addition-fragmentation chain-transfer polymerization (RAFT) and dual crosslinking was employed to fabricate thermo- and pH-dual responsive hydrogels with enhanced mechanical properties. Specifically, two kinds of poly (N-isopropyl acrylamide) (PNIPAM)-based well-defined thermo-sensitive polymers, denoted as PH and PG, consisting of β-cyclodextrin (β-CD) and adamantane (Ad) groups were synthesized via RAFT copolymerization. A facile mixing of PH and PG with commercially available adipic dihydrazide led to the construction of hydrogels via host-guest interactions and dynamic acylhydrazone bonding. The resulting hydrogels not only exhibited pH-switchable sol-gel transitions and temperature-dependent swelling behaviors, but also possessed good stretchability (fracture stress up to 39.97 kPa) and self-healing property (self-healing efficiency above 90%). Notably, the resulting hydrogels showed pH-responsive controlled spatiotemporal release administration of rhodamine B due to the presence of dynamic acylhydrazone links. Together with good cytocompatibility, the hydrogels developed herein represent promising candidates for potential transdermal drug delivery.

A biodegradable injectable fluorescent polyurethane-oxidized dextran hydrogel for non-invasive monitoring.

Hydrogels have been extensively used in the field of biomedical engineering. In order to achieve non-invasive and real-time visualization of the in vivo status of hydrogels, we designed a fluorescent polyurethane-oxidized dextran (PU-OD) hydrogel with good injectability and self-healing properties, which was cross-linked from a tetraphenyl ethylene (TPE)-containing fluorescent polyurethane emulsion with oxidized dextran by dynamic acylhydrazone bonds. The hydrogel can be used as a visual platform for drug delivery as well as monitoring its own degradation. The network structure of the hydrogel gave it drug-loading capability, and the acylhydrazone bond enabled its pH-responsive drug release. Meanwhile, the PU-OD hydrogel could undergo fluorescence resonance transfer with doxorubicin hydrochloride, showing its potential application in monitoring drug release. In addition, fluorometric and weighing methods were performed to monitor the degradation behavior of the hydrogels in vivo and in vitro, respectively, showing that the non-invasive fluorometric method can be consistent with the invasive weighing method. This work highlights that the introduction of aggregation-induced emission molecules into polyurethanes provides a visual platform that allows for non-invasive monitoring of the material without affecting its own function, which is convenient and less damaging to the body or animals. Consequently, it possesses excellent and promising potential in biomedical materials technologies.

Dynamic covalent polymer engineering for stable and self-healing perovskite solar cells.

Perovskite films are susceptible to degradation during their service period due to their weak mechanical properties. Acylhydrazone-bonded waterborne polyurethane (Ab-WPU) was employed as dynamic covalent polymer engineering to develop self-healing perovskite solar cells (SHPSCs). Ab-WPU enhances the crystallinity of the perovskite film, passivates the defects of the perovskite film through functional groups, and demonstrates promising flexibility and mild temperature self-healing properties of SHPSCs. The champion efficiency of SHPSCs on rigid and flexible substrates reaches 24.2% and 21.27% respectively. The moisture and heat stability of devices were improved. After 1000 bending cycles, the Ab-WPU-modified flexible device can be restored to an efficiency of over 95% of its original efficiency by heating to 60 °C. This is because the dynamic acylhydrazone bond can be activated to repair perovskite film defects at a mild temperature of 60 °C as evidenced by in situ AFM studies. This strategy provides an effective pathway for dynamic self-healing materials in PSCs under operational conditions.

Honokiol@PF127 crosslinked hyaluronate-based hydrogel for promoting wound healing by regulating macrophage polarization

Bacterial infection, oxidative stress and inflammation are the main obstacles in wound healing. Hydrogels with moist and inherent properties are beneficial to wound healing. Here, we fabricated a honokiol-laden micelle-crosslinked hyaluronate-based hydrogel by simply mixing honokiol-laden PF127-CHO micelles, 3,3′-dithiobis(propionohydrazide) grafted hyaluronic acid and silver ions. PF127 could not only effectively load hydrophobic small molecules but also be macromolecular crosslinker for preparing hydrogels. Hyaluronic acid plays an essential role in wound healing processes including regulating macrophage polarization towards M2 phenotype. The chemical dynamic acylhydrazone crosslinking and physical crosslinking among PF127-CHO micelles constructed hydrogel's networks, which endowed hydrogel with excellent self-healing properties. PF-HA-3 hydrogel also exhibited outstanding antioxidant and antibacterial capabilities. In a full-thickness skin defect model, this degradable and biocompatible hydrogel could promote wound healing by remodeling wound tissues, regulating M2 polarization and angiogenesis. In summary, this inherent multifunctional hydrogel will provide a promising strategy for designing bioactive compounds-based wound dressings.

Dual-crosslinked hyaluronic acid hydrogel with self-healing capacity and enhanced mechanical properties

Self-healing hydrogels can repair their cracks, and restore their original properties. However, self-healing hydrogels usually face low mechanical strength and poor stability. By the dual crosslinking strategy, a self-healing hyaluronic acid-based hydrogel with enhanced strength was fabricated by dynamic acylhydrazone linkages between aldehyde-modified maleic sodium hyaluronate and 3,3′-dithiobis (propionylhydrazide) and subsequent photopolymerization among maleic groups in the hydrogel network. The hydrogels exhibit fast gelation and excellent self-healing capability due to the dynamic and reversible characteristics of acylhydrazone and disulfide linkages. Furthermore, the dual crosslinking increase the mechanical strength of the hydrogels and prolong their stabilization time. Swelling behaviors, morphology, and mechanical properties could be adjusted by altering the molar ratio of –NH–NH2/–CHO. Besides, the hydrogels displayed interesting pH-responsiveness and cytocompatibility. The hydrogels have potential applications in cell culture, drug delivery, and 3D bioprinting.

“In Water” Fabricated and Recycled Covalent Adaptable Acylhydrazone Thermosets

AbstractThermosetting resins play an increasingly important role in daily life due to their good mechanical properties. However, they can hardly be recycled and reused, leading to severe environmental pollution. A very promising solution to this dilemma is to develop sustainable thermosets from biomass that can be recycled and reprocessed on demand under environmentally friendly conditions. In this study, sustainable thermosets based on dynamic acylhydrazone bonds made from biomass acid derivatives is reported. The sustainability of the presented acylhydrazone biomass covalent adaptable networks (CANs) thermosetting resins is summarized as follows: 1) The raw materials are renewable resources; 2) the resins can be recycled to maintain their mechanical properties, which can considerably extend their service life; and 3) the material preparation and recycling can be conducted in the mixed solvent of water/ dimethyl sulfoxide (7/3), which excellent reduced the use of organic solvents. In addition, these acylhydrazone CANs thermosets have excellent mechanical performance and outstanding heat resistance. The acylhydrazone bonds in the thermoset networks can enhance the hydrophobicity and water resistance of the acylhydrazone CANs thermosets. Taken together, these acylhydrazone CANs thermosets which are fabricated and recycled in the mixed solvent provide a solution to developing sustainable materials.

Self-Healing Hyaluronic Acid Nanocomposite Hydrogels with Platelet-Rich Plasma Impregnated for Skin Regeneration.

The development of natural hydrogels with sufficient strength and self-healing capacity to accelerate skin wound healing is still challenging. Herein, a hyaluronic acid nanocomposite hydrogel was developed based on aldehyde-modified sodium hyaluronate (AHA), hydrazide-modified sodium hyaluronate (ADA), and aldehyde-modified cellulose nanocrystals (oxi-CNC). This hydrogel was formed in situ using dynamic acylhydrazone bonds via a double-barreled syringe. This hydrogel exhibited improved strength and excellent self-healing ability. Furthermore, platelet-rich plasma (PRP) can be loaded in the hyaluronic acid nanocomposite hydrogels (ADAC) via imine bonds formed between amino groups on PRP (e.g., fibrinogen) and aldehyde groups on AHA or oxi-CNC to promote skin wound healing synergistically. As expected, ADAC hydrogel could protect and release PRP sustainably. In animal experiments, ADAC@PRP hydrogel significantly promoted full-thickness skin wound healing through enhancing the formation of granulation tissue, facilitating collagen deposition, and accelerating re-epithelialization and neovascularization. This self-healing nanocomposite hydrogel with PRP loading appears to be a promising candidate for wound therapy.

Self-Healable and Super-Tough Double-Network Hydrogel Fibers from Dynamic Acylhydrazone Bonding and Supramolecular Interactions.

Macroscopic hydrogel fibers are highly desirable for smart textiles, but the fabrication of self-healable and super-tough covalent/physical double-network hydrogels is rarely reported. Herein, copolymers containing ketone groups were synthesized and prepared into a dynamic covalent hydrogel via acylhydrazone chemistry. Double-network hydrogels were constructed via the dynamic covalent crosslinking of copolymers and the supramolecular interactions of iota-carrageenan. Tensile tests on double-network and parental hydrogels revealed the successful construction of strong and tough hydrogels. The double-network hydrogel precursor was wet spun to obtain macroscopic fibers with controlled drawing ratios. The resultant fibers reached a high strength of 1.35 MPa or a large toughness of 1.22 MJ/m3. Highly efficient self-healing performances were observed in hydrogel fibers and their bulk specimens. Through the simultaneous healing of covalent and supramolecular networks under acidic and heated conditions, fibers achieved rapid and near-complete healing with 96% efficiency. Such self-healable and super-tough hydrogel fibers were applied as shape memory fibers for repetitive actuating in response to water, indicating their potential in intelligent fabrics.

Injectable self-healing cellulose hydrogel based on host-guest interactions and acylhydrazone bonds for sustained cancer therapy

Tumor local chemotherapy employing injectable hydrogel reservoirs is a promising platform to achieve precise drug administration. However, balanced injectability, pH-responsiveness and long-term hydrolysis resistance of self-healing hydrogels remain appealing challenges. Herein, a modular preassembly strategy combining host-guest interactions with dynamic acylhydrazone bonds, was exploited to fabricate injectable cellulose-based hydrogels (CAAs) dressed with self-healing properties, pH-responsiveness and hydrolytic degradation resistance. Attributed to the host-guest interaction between β-cyclodextrin (CD) and 1-adamantane (AD), the hydrogels exhibited injectability, self-healing properties (healing efficiency of 97.5%) and rapid recovery (< 10 min) without external stimuli in physiological environment. Moreover, the hydrogels equipped with dynamic acylhydrazone linkages underwent slow hydrolytic degradation (> 30 days) and pH-responsive behavior, endowing the hydrogels with precise spatiotemporal drug release administration. The in vivo application of CAA as a carrier was studied using doxorubicin (DOX) model drug, and the results shows that using CAA as DOX carrier not only greatly enhances the anti-tumor efficacy of DOX, but also reduced the side effects of DOX. Statement of significanceWith the preassemble approach combining host-guest interactions with dynamic acylhydrazone bonds, this work demonstrated a multi-functional self-healing hydrogel as drug carrier developed by using natural polysaccharides, which offers a new avenue for the high-value utilization of biomass. The strategy demonstrated in the present work may also supply a pathway for the preparation and regulation of hydrogels as intelligent biomedicine materials.

Antibacterial Hydrogel with Self-Healing Property for Wound-Healing Applications.

Hydrogels with inherent antibacterial ability are a focus in soft tissue repair. Herein, a series of antibacterial hydrogels were fabricated by quaternized N-[3-(dimethylamino)propyl] methacrylamide (quaternized P(DMAPMA-DMA-DAA)) bearing copolymers with dithiodipropionic acid dihydrazide (DTDPH) as cross-linker. The hydrogels presented efficient self-healing capability as well as a pH and redox-triggered gel-sol-gel transition property that is based on the dynamic acylhydrazone bond and disulfide linkage. Furthermore, the hydrogels showed good antibacterial activity, biocompatibility, degradability, and sustained release ability. More importantly, the in vivo experiments demonstrated that the hydrogels loaded with mouse epidermal growth factor (mEGF) significantly accelerated wound closure by preventing bacterial infection and promoting cutaneous regeneration in the wound model. The antibacterial hydrogels with self-healing behavior hold great potential in wound treatment.