Production Of Dyes Research Articles

Potential of New Plant Sources as Raw Materials for Obtaining Natural Pigments/Dyes

Natural dyes can be extracted from fruits, flowers, leaves, and roots. Exploring new sources of natural dyes, especially from underutilized plants, emerges as a promising strategy. The main advantages of exploiting unconventional plants include local availability, specialty food production, cultural significance, sustainable production, technological feasibility, and new fundamental insights. Finding and exploiting such underutilized plants is significant as unfavorable climatic and human conditions put natural vegetation at risk worldwide. Thus, this study aims to review plants with potential applications as natural dyes and pigments, highlighting their potential applications, benefits, and prospects. An integrative review was conducted by searching Web of Science, ScienceDirect, and SpringerLink for all studies published up to December 2024. For this review, a total of 133 references that presented the information and data of interest to the authors were selected. This review highlighted their potential applications in food, cosmetic, pharmaceutical, and textile industries. Despite the growing interest in natural dyes, challenges related to their stability, seasonality, and extraction efficiency continue to limit their commercial use. However, advancements in extraction technologies have improved the applicability of these compounds. Additionally, utilizing underexplored plant sources presents a strategic opportunity to diversify dye production, reduce reliance on traditional sources, and promote more sustainable practices.

Enhanced production of alizarin type anthraquinone dye achieved through biotransformation of Oldenlandia umbellata L. using different strains of Agrobacterium rhizogenes

Enhanced production of alizarin type anthraquinone dye achieved through biotransformation of Oldenlandia umbellata L. using different strains of Agrobacterium rhizogenes

3-methyl-4-nitrophenol disturbs the maternal-to-zygotic transition of early embryos by damaging mitochondrial function and histone modification.

3-methyl-4-nitrophenol (PNMC), a chemical prevalent in various industries for drug, dye, and leather production, also serves as a primary byproduct of organophosphate insecticides. Despite its global recognition as an endocrine disruptor with documented reproductive toxicity, its detrimental impact on preimplantation embryonic development has yet to be thoroughly investigated. In this study, through the in vitro culture of mice embryos, it was initially observed that even low concentrations of PNMC exposure led to a significant reduction in blastocyst formation and a sharp decline in the ratio of inner cell mass within the blastocysts. SMART-seq2 transcriptome sequencing further confirmed that PNMC treatment disrupted global gene expression in 2-cell embryos, with differentially expressed genes enriched in multiple signaling pathways, including those related to autophagy, apoptosis, fertilization, embryonic development, transcription, and mRNA processing. Integration of transcriptome data with open databases revealed that both zygotic genome activation genes and maternal factors experienced significant transcript-level disruptions. Moreover, the study demonstrated that these gene expression changes were closely associated with mitochondrial dysfunction, evidenced by diminished mitochondrial membrane potential, reduced ATP production, aberrant expression of mitochondria-related genes, increased ROS accumulation, and heightened DNA damage in PNMC-treated embryos. Additionally, PNMC exposure induced defects in histone modification, as shown by altered levels of H3K9me3 and H3K27me3, H3K9ac and H3K27ac. Lastly, the findings indicated that PNMC triggered apoptosis in embryos, validated by elevated BAX and CASPASE3 expression, alongside positive TUNEL staining. In summary, PNMC exposure impairs the maternal-to-zygotic transition, likely through mitochondrial dysfunction and histone modification, culminating in developmental arrest and apoptosis in mouse preimplantation embryos.

Analysis of the production of indigo dyeing products

Analysis of the production of indigo dyeing products

Facile green treatment of mixed cellulose ester membranes by deep eutectic solvent to enhance dye removal and determination.

Synthetic dye production and the consequent generation of dye-rich wastewater are major concerns of water quality in many countries. We developed a sustainable approach with deep eutectic solvent (DES) treatment to enhance the efficiency of mixed cellulose ester (MCE) membrane-based dye removal material. The DES composition and treatment conditions were optimized, and the treated membranes were comprehensively characterized. DES-treated membranes exhibited improved morphology, surface properties, and superior dye adsorption capabilities. Our study revealed that the adsorption process was chemically controlled and driven by electrostatic and hydrogen bond interactions. Thermodynamic analysis confirmed the endothermic and spontaneous nature of the adsorption process. Moreover, the treated membranes exhibited good separation performance for dye/salt mixtures. Additionally, we demonstrated selective adsorption of cationic dyes over anionic dyes using these treated membranes. This selectivity enabled the development of a membrane solid-phase extraction (MSPE) method for quantification of trace amount of dyes. Compared with other methods, DES-treated MCE membranes present a promising solution for efficient dye quantification and removal, offering a green and effective strategy to address water pollution stemming from synthetic dyes. Additionally, this study provides a novel strategy for green chemistry modification of cellulose-based materials.

Clean production and dyeing of fluorescent yellow dyes based on naphthalic anhydride in supercritical carbon dioxide

Supercritical carbon dioxide (scCO2) was selected as the medium for the synthesis and dyeing of four naphthalimide dyes (Y-1, Y-2, Y-3, and Y-4). They were synthesized through the reaction of 4-bromo-1,8-naphthalene dianhydride with various amines. These dyes demonstrated good photophysical properties and thermal stability, aligning with the prerequisites for scCO2 dyeing. Consequently, the scCO2 dyeing of polyester fabrics using these four dyes was investigated, and the optimal dyeing conditions were established in the range of 1820 MPa, 120130℃, and 8090 min. Surface analysis confirmed the uniform distribution of the dyes on the fabrics, with no significant damage to the fabric structure under the dyeing conditions. Additionally, the dyed fabrics exhibited good color fastness to soap washing and friction with level 4 or above. Overall, the clean preparation and dyeing of naphthalimide dyes hold promise for reducing environmental pollution.

Ixora coccinea (jungle geranium): A dual-functioning agent for crystal violet removal from wastewater and dye production

ABSTRACT This study explores the red Ixora coccinea flower, also known as jungle geranium, as an effective adsorbent for removing crystal violet (CV) from synthetic and industrial effluents. Characterization techniques such as FESEM, BET surface area, FTIR, and pHZPC reveal the particle structure and characteristics. The adsorption performance is evaluated using various physicochemical parameters, including temperature, concentration, pH, and contact time. The results indicate rapid adsorption, with 93.73% removal within 15 min and 97.63% at equilibrium after 30 min at neutral pH. The maximum adsorption capacity is 129.23 mg/g. The pseudo-second-order kinetics model (R2 = 0.999) confirms the chemisorption process, while the Langmuir model (R2 = 0.999) describes the adsorption pathway. Thermodynamic analysis indicates an exothermic and spontaneous process, with ∆H values between −59.43 and −46.51 kJ/mol and ∆G values from −8.63 to −5.67 kJ/mol. The spent adsorbent can be regenerated in up to 75.26% of cases using a 1:1 MeOH/H2O solution. Practical applications demonstrate effective CV removal in industrial wastewater (54.21%). This work highlights the potential of Ixora coccinea flower extract for both wastewater treatment and as a source of natural dye.

Synthesis and Characterization of Schiff Base Compounds Containing Mono and Disulfonic Groups

Aromatic mono- or diamine compounds containing sulfonic groups are extensively utilized in the production of textiles, dyes, and polymers. Schiff base compounds are synthesized through the condensation reactions of these types of compounds with aromatic aldehydes or ketones. Due to their multifunctional nature, these Schiff base compounds serve as precursors in the synthesis of poly(ester)s, poly(ether)s, poly(urethane)s, and poly(azomethine)s. The Schiff base compounds were 2,5-bis((E)-(2-hydroxybenzylidene)amino)benzene sulfonic acid (SCH1) and 4,4'-bis((E)-(2-hydroxybenzylidene)amino)-[1,1'-biphenyl]-2,2'-disulfonic acid (SCH2). The yields of SCH1 and SCH2 were calculated to be 85% and 80%, respectively. Structural confirmation was achieved using FT-IR, UV-Vis, 1H NMR and 13C NMR measurements. Further characterization through fluorescence spectroscopy (PL), thermogravimetric analysis (TGA) and cyclic voltammetry (CV) revealed high thermal stability of the compounds, attributed to the phenyl groups in their structures. TGA measurements indicated the initial decomposition temperatures for SCH1 and SCH2 at 298°C and 347 °C, respectively, with the residual masses of 34.64% and 41.24% at 1000 °C.

IoT real-time monitoring system implemented to observe sunlight-influenced methylene blue degradation by AC/HAp.

Activated carbon (AC) and hydroxyapatite (HAp) composite were successfully prepared via mechanical mixing and exhibited a crushed snack-like morphology compared to the well-defined pore structure of pure AC. The purpose of this research was to explore the potential of AC/HAp for the degradation of methylene blue (MB) dye and hydrogen production. Despite the morphological changes, AC and AC/HAp achieved a high MB degradation rate (92.75 and 82.05) % under sunlight, at a temperature of 26.24°C and pH of 4.18, and showed optimum hydrogen production (3579mol/g.h). These performances are suspected from the decrease in crystallite size and narrowed and confirmed by image processing results with RBG enhancement showing more light transmitted by the solution. Increasing in indicates the potential for improved light interaction. IoT-enabled real-time monitoring system demonstrates its efficacy in facilitating wastewater treatment and hydrogen production, offering a promising solution for aquatic ecosystem restoration.

The application of electroflocculation (EC) technology in printing and dyeing wastewater

Abstract. Industrial wastewater contains a large number of toxic pollutants, and the total consumption of dyes in the global textile printing and dyeing industry exceeds 587,000 tons per year. Duo to the process of dye production is more complex, the application of processes and materials are more, so many production links will produce a variety of waste, garbage, and the relevant waste dye into the river, lake and sea is up to millions of kilograms. It can be seen that textile printing and dyeing wastewater is the main source of water environmental pollution. If not treated in time, it will not only affect the production efficiency, but also harm the surrounding ecological environment and pose a serious threat to human survival. Scientific and effective intervention of textile industry wastewater and reasonable selection of textile industry wastewater intervention mode are related to the sustainable development of the industry. In recent years, electroflocculation (EC) technology has gradually become a research hotspot due to its advantages of high removal rate, simple operation, small dosage of chemicals, and small impact on the environment, including the intervention of wastewater. This paper will describe the main mechanism of EC and the application status of EC technology is discussed in detail, the limitations and future development trend of this technology are also proposed.

Fusarium verticillioides pigment: production, response surface optimization, gamma irradiation and encapsulation studies

BackgroundNatural pigments are becoming more significant because of the rising cost of raw materials, pollution, and the complexity of synthetic pigments. Compared to synthetic pigments, natural pigments exhibit antimicrobial properties and is less allergic. Pigments from microbial sources could easily be obtained in an inexpensive culture media, produced in high yields, and microbes are capable of producing different colored pigments. Searching for new sources for natural pigments to replace synthetic ones in food applications has become an urgent necessity, but the instability of these compounds is sometimes considered one of the obstacles that reduce their application. Encapsulation provides an ideal solution for natural dye protection through a controlled release strategy. Thus, this study aims at isolation of several soil fungi and subsequent screening their pigment production ability. The chosen pigment-producing fungal strain underwent full identification. The produced pigment was extracted with ethyl acetate and estimated spectrophotometrically. As there is a necessity to obtain a high pigment yield for efficient industrial application, the best production medium was tested, optimum conditions for maximum dye production were also investigated through the response surface methodology, and gamma irradiation was also employed to enhance the fungal productivity. Encapsulation of the produced pigment into chitosan microsphere was tested. The pigment release under different pH conditions was also investigated.ResultsA new strain, Fusarium verticillioides AUMC 15934 was chosen and identified for a violet pigment production process. Out of four different media studied, the tested strain grew well on potato dextrose broth medium. Optimum conditions are initial medium pH 8, 25 °C-incubation temperature, and for 15-day incubation period under shaking state. Moreover, a 400 Gy irradiation dose enhanced the pigment production. Chitosan microsphere loaded by the pigment was successfully prepared and characterized by infrared spectroscopy and scanning electron microscopy.ConclusionThis irradiated Fusarium strain provides a more economically favorable source for production of a natural violet dye with an optimum productivity, enhanced yield, and improved properties (such as, enhanced stability, controlled release, and bioaccessibility) by encapsulation with chitosan for efficient application in food industry.

Modelling and optimizing secondary metabolites production in Spirodela polyrhiza using machine learning

Spirodela. polyrhiza as one of the potential feedstocks for industrial natural dye and pharmaceutical products, efficient process to produce from it were scarce. Here, Artificial Neural Network (ANN) and Genetic Algorithm (GA) were employed for modelling and optimization respectively. Current study focus on establishing useable models and anticipating the optimal concentration of nitrate (NO3−), phosphate (PO43−) and ammonium (NH4+) to give highest relative growth rate (RGR) and produce maximum amount of anthocyanins, chlorophylls, phenolic compounds and antioxidants. In short, 12 and 11 neurons in hidden layer were found to be most suitable neuron numbers used for the modelling of growth and secondary metabolites respectively. All the models exhibited a high degree of robustness and performance with a high r2 value (higher than 0.75) and a low mean squared error (lower than 0.05). GA also anticipated the best concentration of macronutrients to achieve the maximum growth and metabolites production with significant low errors (below 7 %). The employment of machine learning was useful for such applications.

Adsorption of Carotenoids, Chloride, and Sulfate from Annatto Dye Agro-Industrial Effluent

<p>Natural dyes, such as annatto, are widely used in the food and pharmaceutical industries. However, the effluents from annatto dye production pose environmental challenges due to the presence of residual dye components, organic matter, and dissolved inorganic species (chloride and sulfate). This study, the first attempt in the literature to deal with real annatto dye effluent, aimed to evaluate the use of activated carbon for the removal of carotenoids (norbixin), chloride, and sulfate. A 2³ full-factorial design was employed to optimize the adsorption process parameters (temperature, pH, and adsorbent mass). The results showed that up to 90% of carotenoids and chloride could be removed under optimized conditions (low temperature and pH). Sulfate removal was more strongly influenced by the pH of the medium, with a maximum removal of 53%. The adsorption process was well-described by empirical models, though traditional isotherm models did not adequately represent the experimental data. Hypotheses that support isotherm models such as pore homogeneity, monolayer formation, constant internal diffusion were not satisfied. This study demonstrates the potential of activated carbon adsorption as a feasible method for treating food industry effluents containing high loads of carotenoids and inorganic salts.</p>

Utilization of Aspergillus niger for the fermentative production of azaphilone dye in YEPB medium.

The current research focuses on the production and optimization of a natural yellowish-brown Azaphilone dye using Aspergillus niger. A variety of culture media were tested to ascertain the best conditions for dye synthesis. The formation of the yellowish-brown dye was confirmed by a color shift in the reaction mixture, and UV-Vis spectroscopy detected the dye at 450nm. Static conditions were found to be more favorable than shaking for higher dye yields, and fed-batch fermentation was more effective than batch fermentation. Maximum dye production was achieved after 28days of incubation. Factors such as temperature, pH, and inoculum percentage were shown to influence dye synthesis, with the highest production (2.5ml) occurring at 30°C, pH 7, and a 3% spore suspension in yeast extract peptone broth (YEPB) medium under static conditions. Gas chromatography-mass spectrometry (GC-MS) analysis validated the presence of Azaphilone dye in the culture filtrate. The dye was successfully applied to a pretreated cotton cloth. These findings advance our understanding of optimizing fungal dye production for sustainable and eco-friendly textile coloration applications. This study appears to be the first of its kind to report azaphilone dye production by A. niger in the YEPB medium.

EFFECT OF BORIC ACID ADDITION ON ANTIBACTERIAL DYE PRODUCTION

In this study, the use of interior dyes with antibacterial properties was examined. Boric acid (H3BO3), which is a cheap and easily obtainable boron compound from our country's resources, has an antibacterial effect. Within the scope of the study, firstly, boric acid mixtures were prepared in appropriate concentrations and antibacterial dye production studies were carried out by adding them to indoor dyes. The antibacterial properties of the obtained dye were investigated through the inhibition effect on E. coli bacteria and its antibacterial activity was tested, and then the brightness, density, film thickness, scorching, pencil hardness and drying time tests of the dyes and the suitability of their characteristics to TS 5808/2012 and the applicability of these dyes were evaluated. The results showed that the antibacterial effect of boric acid did not have a negative effect on the dye quality, but maintained the standard values of the dye.

Highly energy-efficient degradation of simulated dye wastewater by array type underwater bubble discharge plasma: Key factors identification and degradation pathways

The extensive use of dyes presents a significant safety concern for the reuse of water resources, highlighting the critical need for a rapid and efficient degradation method for dye mixtures in wastewater. This study introduces a degradation system based on an array type underwater bubble discharge plasma specifically designed to treat a dye mixture wastewater containing methylene blue (MB) and methyl violet 2B (MV2B). Analysis of the optical and electrical characteristics reveals that the device experiences minimal temperature increase, with the highest intensity of the band associated with N2 in the emission spectra, and discharges occurring predominantly during the rising and falling edges of a pulse cycle. Experimental results demonstrate that the most effective degradation efficiencies (MB = 94.83%, MV2B = 93.48%) are achieved at an initial dye concentration of 50 mg/L, a power supply frequency of 6 kHz, an air flow rate of 2.5 SLM and an initial electrical conductivity of 50 μS/cm. The degradation of dyes is primarily attributed to the demethylation process of O3(aq) and other species. Toxicity analysis indicates that the plasma degradation process significantly reduces the toxicity of the intermediate products of dyes. This study not only presents a novel approach for treating high concentration mixed dye wastewater, but also provides valuable insights for future research in this area.

Identification of Omitted Pollutants in Environmental Water via In Situ Thin-Film Microextraction.

Sample preparation is an inevitable step in the screening workflow for the identification of unknown pollutants in the aquatic environment. However, the possible loss of pollutants during sample preparation has aroused serious concern but remains not effectively resolved. This study shows that high-risk pollutants omitted in solid-phase extraction (SPE) can be identified via in situ thin-film microextraction (TFME) coupled with high-resolution mass spectrometry. It was observed that a total of 541 features showed higher mass spectrometry signal intensity by using in situ TFME in comparison with SPE. Subsequently, 28 compounds were identified from the features with higher intensity by comparing the recorded tandem mass spectra with the online database and validating with standards. Notably, six out of these compounds were completely omitted using SPE, including a dye, drug, and industrial product. It was confirmed that the lower extraction efficiencies of SPE were attributed to the limited sample volumes, the losses of compounds during sample transportation and storage, and the entrapment of compounds in SPE columns. This study demonstrates that in situ TFME should be used as a supplementary technique to SPE for extending the coverage of pollutants in the screening workflows.

Effect of chromophore type on efficiency of reactive dye removal using polyamidoamine dendrimer

AbstractIn this study, by selecting a sample of dyes from a wide variety of chromophores used in the production of reactive dyes (monoazo, bisazo, metal azo, phthalocyanine, anthraquinone, copper formazan and triphendioxazine), the colour removal performance of polyamidoamine (PAMAM) dendrimer in reactive dyes was statistically evaluated depending on the dye chromophore groups (and accordingly molecular weight and number of sulfo groups of the dye), dendrimer concentration and centrifugation time. When all the results were examined, it was observed that the colour removal efficiency was significantly lower in metalazo and copper formazan‐based reactive dyes, whereas colour removals ranging from 93% to 98% could be obtained in bisazo, triphendioxazine, monoazo, anthraquinone and phthalocyanine dyes. With the use of dendrimer, the best colour removal efficiencies occurred at pH 3. However, it was found that the centrifugation time of 15 min is sufficient for all dyes, and a longer centrifugation does not provide any additional benefit in terms of colour removal.

Harnessing the hidden environmental power of Bjerkandera adusta laccase: Sustainable production, green immobilization, and eco-friendly decolorization of mixed azo dyes

This research unveils the untapped potential of laccase, derived from the Bjerkandera genus, utilizing it in an immobilized system aimed at detoxifying harmful azo dye effluents from the textile industry, thus contributing to environmental protection. Marking a pioneering achievement, we recorded the highest laccase activity at 94.52 U/g cultivating B. adusta's mycelium on brewer's spent grain, enhanced with lignocellulosic waste, under meticulously optimized conditions −2.69 g of alkali-pretreated beech sawdust, 0.91 g of cypress cone, and 8-day incubation period. The harvested laccase was subjected to an immobilization process on alkali-pretreated beech sawdust, where, through optimization, we established the ideal conditions (30 mg of carrier, pH 7, and 3.5 h), achieving an immobilization efficiency of 72.24% with a residual activity of 57.64%. Remarkably, both free and immobilized forms of the B. adusta TMF1 laccase enzyme demonstrated formidable efficacy in decolorizing a mix of three distinct azo dyes (Orange G, Eriochrome Black T, and Congo Red), eliminating over 63% of the coloration within just 30 min. The immobilized laccase showed consistent performance across four decolorization cycles. Moreover, the breakdown products of the azo dye mix were analyzed using the HPLC method, complemented by evaluations of potential antimicrobial activity, phytotoxicity and cytotoxicity, revealing a non-toxic composition without cytotoxicity, highlighting the process's safety for environmental release. The significance of this research is reflected in the distinguished construction of a green biocatalyst acting as a stable and time-efficient in remediation of targeted azo dye pollution from the textile industry following the principles of circular economy.

Rational Design of Co(II)‐Pyridine‐Decorated Reduced Phosphomolybdate Photocatalysts for Efficient Aniline Oxidation under Mild Conditions

Comprehensive SummaryThe conversion of aniline to nitrosobenzene (NSB), a key step in the synthesis of important chemicals, demands the development of environmentally friendly photocatalysts that operate under mild conditions. This process is driven by the need for sustainable methods, given toxic nature of aniline and its central role in the production of dyes, pharmaceuticals, and agricultural chemicals. This study reports on the synthesis of three reduced phosphomolybdate compounds with Co(II) centers and polypyridine ligands, which show great potential as photocatalysts for the mild oxidation of aniline to NSB. The synthesis uses 30% H2O2 as the oxidant and the compounds as the sole catalysts, achieving high conversion rates and selectivity without additional additives or photosensitizers. The integration of multidentate ligands with {P4Mo6} clusters significantly reduces the band gap, facilitating visible‐light‐driven photocatalysis. The adsorption interaction between coordinated water molecules and Co ions and H2O2 is found to enhance the conversion rate of aniline, a finding supported by DFT analyses.