Green Dwarf Coconut Research Articles

Biodiesel synthesis from low cost biomass wastes and its cost assessment inducing process optimization

This study employed low-cost biomass wastes for the synthesis of biodiesel that is cost-effective and environmentally friendly. The major raw material (oil) was obtained by steam distillation (SD) from Croton heliotropiifolius Kunth leaf (CHKL) and was characterized for its aptness for biodiesel production. Dwarft green coconut husk ash (DGCHA) was used as a bio-adsorbent for acid value reduction of Croton heliotropiifolius Kunth leaves oil (CHKLO). A novel, highly potassium-based catalyst was derived from Karpuravalli banana peels (KBP), calcined, and characterized using TGA, ZETA, FTIR, SEM-EDX, XRF-FS, and BET analysis. Biodiesel was synthesized using a microwave-assisted method, characterized, and compared with the recommended standard. The catalytic strength of the calcined Karpuravalli banana peel powder (CKBPP) was tested using a reusability test, and the cost evaluation of production was estimated. Results showed that the CHKL was rich in oil (43% wt./wt.), and the oil is highly acidic (5.23 mg KOH/g oil). At high particle size, the dwarf green coconut husk ash (DGCHA) bagasse reduced the acid value to a minimum (1.4 mg KOH/g oil) at 3 days. The developed novel catalyst from CKBPP indicated high potassium-calcium contents for base transesterification. Process optimization indicated that the predicted response data of 95.285% (wt./wt.) at T1 = 90 min, T2 = 60 oC, T3 = 4.5% (wt.), and T4 = 9 (vol./vol.) was validated in triplicate, and the average data value of 95.10% (wt./wt.) was established. Dataset on the quality of biodiesel showed that the produced biodiesel properties were in line with recommended standards. Economic appraisal data showed that the cost of producing 20 L of CHKLOB (biodiesel) was $4.73 at 1,500 to $1. The study concluded that the production of biodiesel from waste can be cost-effective and environmentally friendly if wastes are harness.Graphical

Effect of Biochar on Dwarf Green Coconut Orchard Yield and Irrigation Water Productivity

ABSTRACT The high irrigation water demand of coconut trees in semiarid regions makes rational use crucial for crop sustainability. Additionally, it produces residues that could improve the soil water holding capacity. This research aimed to evaluate the application of coconut shell biochar to an irrigated dwarf green coconut palm orchard. The study was carried out from January 2019 to December 2021 at Campo Experimental do Curu, Paraipaba, Brazil, belonging to Embrapa Agroindústria Tropical. The experiment consisted of a completely randomized design with five treatments and four replications and included the application of 0, 5, 10, 20, or 40 g of biochar per kg of soil, equivalent to 0.0 kg, 0.5 kg, 1.0 kg, 2.0 kg or 4.0 kg of biochar per plant, respectively. The following biometric variables were evaluated: number of leaves per plant (NL), plant height (PH), number of leaflets per leaf (LFT), canopy diameter (CD) and fruit production: number of bunches per plant (NB), number of fruits per plant (NF), volume of coconut water per fruit (CWF) and total soluble solids (TSS) (°Brix), as well as the daily soil moisture average. The results showed beneficial effects on the number of leaves per plant, fruits per plant, soil moisture and irrigation water productivity in terms of liters per fruit, liters per coconut water and fruits per cubic meter (m3) applied with ideal biochar doses estimated to be 1.40 kg, 1.72 kg, 1.79 kg, 1.56 kg, 1.48 and 1.68 kg of biochar per plant, respectively.

Cost Reduction in the Production of Green Dwarf Coconut Palm Seedlings Biostimulated with Bacillus cereus.

The online version contains supplementary material available at 10.1007/s12088-023-01163-9.

Soil physical, chemical and biological properties in Conilon coffee intercropping systems

ABSTRACT Shaded coffee systems may offer a series of benefits, however, studies on shaded cultivation of Conilon coffee crops are still scarce in the literature. This study aimed to evaluate the soil physical, chemical, and biological properties of two Conilon coffee intercropping systems from southeastern Brazil. Two commercial coffee crops – one shaded by macadamia trees and the other by green dwarf coconut trees – were evaluated according to three treatments: coffee plants in the inter-row of the tree species; coffee plants in the same row of the trees; and unshaded coffee. The experimental design was a completely randomized with four replicates. Coffee plants intercropped with macadamia trees, both intra- and inter-row, resulted in less soil compaction than unshaded systems. As for intercropping with green dwarf coconut trees, the unshaded system presented lower soil resistance to penetration. Differences in physical properties between treatments allow no inferences about intercropping systems influence on green coconut trees. Intercropped coffee improves soil chemical properties, resulting in greater soil fertility than unshaded systems, and showed greater soil organisms. These findings indicate that Conilon coffee-macadamia intercropped with tree species represents a promising alternative for sustainable soil management.

Produtividade da água em coqueiro irrigado nas condições de clima tropical úmido na Amazônia Oriental

ABSTRACT: The growing demand for coconut water has led to the expansion of irrigated green dwarf coconut cultivation; however, the inefficient use of water has contributed to the degradation and waste of water resources, making fundamental studies aiming to optimize the use of water. The objective of this study was to determine the physical productivity and water productivity of green dwarf coconut palms under the climatic conditions of Santa Izabel do Pará, eastern Amazonia, Brazil. The experiment was carried out from 2020 to 2021, in a green dwarf coconut plantation spaced 7.5 m x 7.5 m in a triangle, at Fazenda Reunidas Sococo, with an area of approximately 7 ha. Water productivity (WP) was determined both in terms of fruit production (WPf) and coconut water production (WPca), through the relationship of fruit productivity (fruit biomass and water volume) with accumulated evapotranspiration. The dwarf coconut tree presented a total production of 105 and 186 fruits plant-1 and 37 and 62 L plant-1 of coconut water in the years 2020 and 2021. The largest volumes of water and the highest weights occurred during the wettest season. WPf was of 1.87 fruits m-3 (2020) and 2.94 fruits m-3 (2021) and WPca 0.66 L m-3 (2020) and 0.98 L m-3 (2021).

Net radiation partitioning, evapotranspiration, and crop coefficients of the green dwarf coconut in Santa Izabel do Pará, Brazilian Amazon

Net radiation partitioning, evapotranspiration, and crop coefficients of the green dwarf coconut in Santa Izabel do Pará, Brazilian Amazon

Condições meteorológicas afetam o desenvolvimento sazonal e a produção de frutos de coqueiro-anão-verde

ABSTRACT Understanding the green dwarf coconut palm development is essential to improve its management. This study aimed to characterize and understand the seasonality of the green dwarf coconut palm development and yield under rainfed and irrigated conditions in the northeastern region of the Pará state, Brazil. The longitudinal and transverse diameters, fruit mass and yield, in addition to the number of live leaves, live bunches, fruits of the bunches nº 11 and 14 and female fowers were monitored. The coconuts between the bunches nº 20 and 22 showed larger dimensions and biomass (fresh and dry). The partition of the production components varies according to the time of the year, with the liquid albumen mass, which is a component of greater commercial focus, being higher in the rainy season, with lower percentage losses in the irrigated cultivation. The meteorological seasonality plays a key role in fower dropping and fruit abortion, directly contributing to the amount of produced fruits.

Aplicações de gesso e calcário melhoram a fertilidade do solo sob cultivo de coco na Amazônia Oriental, Brasil

ABSTRACT Gypsum and lime application causes chemical modifications in the soil that influence nutrient content, root growth and development of plants. An experiment was carried out in the municipality of Santa Isabel, Pará state, Brazil, to evaluate the chemical changes in the soil and the responses of green dwarf coconut to gypsum and lime at 6 and 12 months after application. The experimental design was completely randomized blocks, with 4 blocks and 7 treatments: T1 = control; T2 = 2000 kg ha-1 lime; T3 = 500 kg ha-1 gypsum; T4 = 2000 kg ha-1 lime + 300 kg ha-1 gypsum; T5 = 2000 kg ha-1 lime + 500 kg ha-1 gypsum; T6 = 2000 kg ha-1 lime + 700 kg ha-1 gypsum; T7 = 2000 kg ha-1 lime and 1000 kg ha-1 gypsum. After 12 months of applying the treatments, the combination of gypsum and limestone was used to reduce aluminum saturation, with values of 0% at a depth of 0.0-10 cm of the soil, as well as increments in sulfur contents and base saturation of the soil, with values of 70% at a depth of 0.0-10 cm of the soil; with positive effects on macronutrients concentrations in leaf tissue and on root growth. Application of agricultural gypsum, associated with lime, is efficient to improve the chemical attributes of the soil and the development of green dwarf coconut plants.

Fertilização com zinco melhora o crescimento e desempenho fotossintético de mudas de coqueiro anão verde

ABSTRACT Zinc is an essential micronutrient for plant metabolism and has been studied in several crops of economic interest. However, there is no evidence of its use in coconut crops. The objective of this study was to evaluate the influence of foliar zinc fertilization with doses of zinc on dwarf green coconut seedlings based on their nutritional status, growth attributes, and gas exchange. The experimental design consisted of randomized blocks, and the treatments consisted of different zinc doses (0, 5, 10, 15, 20, and 25 g per plant) with five replicates. Seedlings were positively influenced by zinc dose, mainly in terms of growth variables and gas exchange. Zinc fertilization reduced iron and manganese content and increased zinc concentrations in the leaf tissue. Zinc fertilization promoted an increase in leaf area, height, shoot dry mass, and total dry mass. Furthermore, zinc fertilization improved photosynthesis, transpiration, stomatal conductance, internal CO2 concentration, instantaneous water use efficiency, and intrinsic water use efficiency. The improvement in these growth indices and gas exchange, in most variables, was up to an estimated dose of approximately 16 g of zinc per plant and decreased with increasing doses.

Integrated soil fertility management using cocoa bean shells improves soil chemical properties, coconut yield and mitigates environmental pollution

One approach to sustain and intensify coconut production is nutrient cycling through an integrated soil fertility management strategy. This strategy may include transferring crop by-products from one farm commodity to another. One such promising by-products identified is cocoa bean shells (CBS), which is in abundance and could serve as an environmental pollutant at Bamiankor, which is a forest zone and an important area for coconut and cocoa productions, located in the Western region of Ghana. Following this, a 42-month integrated soil fertility management field study was conducted to assess improvements in selected soil chemical properties, coupled with vegetative, and reproductive parameters in a Sri Lankan Green Dwarf (SGD) coconut genotype under various fertilizer treatments. The treatments included: no-fertilizer control (CK), nitrogen-phosphorus-potassium (NPK) fertilizer, cocoa bean shells (CBS), and NPK+CBS, which were arranged in a randomized complete block design (RBCD) in three replications. There were appreciable increases in values of soil pH, total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), and available phosphorus (AP). Values in carbon to nitrogen (C:N) ratio after treatment with CBS, however, were significantly reduced. It was observed that collar girth from palms treated with NPK+CBS were significantly higher compared to other treatments. Similarly, number of leaves, percentage of palm inflorescence, and number of nut-load palm−1 year−1 were all significantly higher under NPK+CBS treated palms compared to the other treatments. In conclusion, CBS in combination with NPK fertilizer is recommended as a good strategy to intensify coconut production. However, for a long-term benefit, circularity, cleaner, and sustainable production, addition of sole CBS is advocated. Adoption of this management strategy will also potentially mitigate the negative environmental consequences associated with accumulation of CBS in studied area.

Spatial patterns of the leaf crown borer Eupalamides cyparissias (Cramer, 1775) (Lepidoptera: Castiniidae) in coconut (Cocos nucifera L.) tropical region.

The leaf crown borer Eupalamides cyparissias (Cramer, 1775) is an important pest of coconut (Cocos nucifera L.) and other palms (Arecaceae) of economic importance, attacking the base of leaves, inflorescences, and infructescences, increasing fruit abortion. The objective of this study was to evaluate the spatial correlation of the infestation rate of E. cyparissias in coconut plantation blocks in the Brazilian Amazon, from January to December 2019, in the city of Santa Izabel, Pará, Brazil. The study area is a farm subdivided into 157 blocks of a commercial plantation of the green dwarf coconut. The Local Moran's Index was used to evaluate the existence of spatial autocorrelation of the E. cyparissias infestation rate in the 157 blocks with neighboring blocks. The infestation rate was calculated by the ratio between the number of plants attacked by the borer and the total number of plants in a block. There is a significant correlation of the symptomatology of the attack by E. cyparissias in the blocks of the experimental area, which indicates an aggregated pattern of distribution. There is no significant correlation between the attack by the borer and the age of the coconut tree; however there is a significant correlation between the attack by the borer and proximity to forest areas. These results indicate that forest regions are foci of infestation by the borer in coconut plantations.

Efeito da irrigação com déficit hídrico sustentado sobre a produtividade e qualidade da água dos frutos do coqueiro anão verde

ABSTRACT: This study evaluated the effects of sustained deficit irrigation (SDI) on fruit yield and fruit water quality of dwarf green coconut trees. The experiment was carried out in a commercial orchard located in Camocim, Ceará, Brazil. Four years old coconut trees were irrigated during 29 months, using micro-sprinklers, at irrigation depths equivalent to 55%, 77%, 100% and 131% of crop evapotranspiration (ETc). Green coconut fruits were harvested six months after flower aperture and evaluated for number of fruits per plant, volume of coconut water per fruit and total soluble solids of the coconut water. SDI reduced coconut fruit yield, fruit water volume and coconut water yield. Conversely, SDI increased total soluble solids of the coconut water and irrigation water productivity in terms of fruits and coconut water. Deficit irrigation showed no economic advantage over full irrigation due to the small reduction in irrigation costs compared to the substantial reduction in gross revenue.

Heterogeneity and Spatial Distribution of Inorganic Nitrogen as Nitrate (N-NO3) in Two Soils Dedicated to Green Dwarf Coconut in Guerrero, Mexico

Heterogeneity and Spatial Distribution of Inorganic Nitrogen as Nitrate (N-NO3) in Two Soils Dedicated to Green Dwarf Coconut in Guerrero, Mexico

Beneficial Effect of Biochar on Irrigated Dwarf-Green Coconut Tree

The coconut tree is considered one of the greatest consumers of irrigation water, ranging from 100 to 240 L day−1. The objective of the present study was to evaluate the effect of biochar application on decreased irrigation water needs in a 2-year irrigated dwarf coconut palm orchard field experiment. Biochar was characterized chemically and by electron microscope images. Biochar morphology presented several micropores indicating water retention potential. Amounts of biochar were tested (0, 5, 10, 20, 40 g of biochar per kg of soil), representing 0.0; 0.5; 1.0; 2.0; and 4.0 kg per plant. Micro sprinkler irrigation started following the planting of the 90-day old hybrid dwarf coconut seedlings. The impacts of the application of the biochar on the chemical attributes of the soil, biometry of the coconut plants, water storage in the 0–0.3 m soil layer, and the volume of irrigation water required by treatment were evaluated. After two years (2017 and 2018), the application of the biochar resulted in no statistically significant differences in the chemical attributes of the soil and biometric variables of plants between different treatments. The volume of annual irrigation water per plant versus biochar quantity demonstrated a decreasing effect, due to the increase of soil water storage. The dose of 40 g of biochar per kg of soil presented the highest two-year average soil water retention (0–0.3 m layer) among treatments (34, 36, 34, 38, and 45 mm, respectively), resulting in lower 2-year irrigation water demand (28, 36, 29, 28 and 20 L plant−1 day−1, respectively).

Bacillus cereus Improves Performance of Brazilian Green Dwarf Coconut Palms Seedlings With Reduced Chemical Fertilization.

Coconut production in the Amazon requires the knowledge and development of sustainable technologies to alleviate the detrimental effects of inorganic chemical fertilizers and intensive farming practices. In this study, we investigated the effects of plant growth-promoting rhizobacteria (PGPR) isolated from coconut seedlings on nutrient use efficiency (NUE) and physiological mechanisms related to biomass accumulation of seedlings grown with reduced inorganic fertilizer levels. Of the 96 PGPR isolates tested on rice plants, the isolate Bacillus cereus (UFRABC40) was selected, as it resulted in the most significant gain in growth variables. In a commercial coconut tree nursery, we subjected seedlings to two treatments, both with seven replications: control 100% NPK chemical fertilizer (CF) and B. cereus + 50% NPK CF. The results indicated that the inoculation increased phytohormone levels [190% indole acetic acid (IAA), 31% gibberellic acid GA3, and 17% gibberellic acid GA4] and leaf gas exchange [48% by assimilation of CO2 (A), 35% stomatal conductance to water vapor (gs), 33% transpiration, and 57% instantaneous carboxylation efficiency] in leaves. Furthermore, growth parameters (shoot, root, and total dry weight, height, and diameter) and macro- and micronutrient levels (95% N, 44% P, 92% K, 103 Ca, 46% Fe, 84% B) were improved. Our results show the potential ability of strain Bacillus cereus UFRABC40 to promote the growth performance of coconut seedlings under decreased application of inorganic fertilizers. The application of microbial-based products in coconut seedling production systems improves plants’ physiological performance and the efficiency of nutrient use.

Geostatistical modeling of “lethal coconut palm crown atrophy”, a new disease in the State of Pará

’Lethal Coconut Palm Crown Atrophy’ (LCCA) is a rapidly spreading disease in Brazil, capable of quickly killing coconut trees and threatening the commercial exploration of this plant. The objective of this work was to characterize the spatial and temporal distribution pattern of LCCA in green dwarf coconut commercial plantation areas, located the municipality of Santa Izabel, mesoregion of Northeastern Pará, Brazil. Surveys were carried out at monthly intervals between January 2014 and December 2018, checking for plants with LCCA-characteristic symptoms. Geostatistics was applied to perform spatial-temporal disease estimates based on semivariogram modeling and preparation of ordinary kriging maps. These spatial estimates are conducted through interpolations that characterize data variability in the area. The spherical model yielded the best fit to the spatial distribution of the disease, as it presented the best coefficient of determination (R²), with the range varying between 14m and 45m. The Spatial Dependence Index (SDI) was moderate in the evaluations carried out between 2014 and 2017 (in the 0.26-0.64 range), but not in 2018, when it was strong (0.23). The values of the clustering intensity of LCCA-symptomatic plants were estimated in non-sampled points. The spherical fit model of the data indicates an aggregated distribution pattern, shown by aggregation patches in the plantation, graded by values of dissemination intensity. The kriging maps allowed the observation that the disease expands between plants in the same line, suggesting the possibility of the presence of a short-range vector.

Harvest timing and potassium doses on post-harvest quality of dwarf-green coconut water

Coconut is an expressive culture in tropical climate regions and its yield and fruit quality is directly linked to several factors, such as climatic conditions, fertilizer management, harvesting fruits period and other factors. Aiming to evaluate post-harvest of irrigated green dwarf coconut water quality due to potassium doses and different harvesting time, an experiment was carried out at the Federal Rural University of the Semi-Arid - UFERSA from January to November 2015. The experimental design was split-plot randomized blocks. Plots were 5 potassium doses (0, 150, 300, 450 and 600 g plant-1) and the subplots 4 harvesting time of fruits (5, 6, 7 and 8 months after the opening of the inflorescence), 4 blocks and 2 plants for a total of 40 treatment plants. The experimental area consisted of coconut trees of green dwarf variety with four years old. Were evaluated the titratable acidity, soluble solids, ratio, electrical conductivity, pH, total sugar and levels of potassium, calcium, magnesium, sodium and chlorine from coconut water. Under the conditions studied, higher doses of potassium decreased qualitative characteristics of coconut water, the absence of potassium fertilization coconut trees had better results. The optimal harvest time is between 6 and 7 months old.

Seasonal shifts of arbuscular mycorrhizal fungi in Cocos nucifera roots in Yucatan, Mexico.

The diversity and community structure of arbuscular mycorrhizal fungi (AMF) associated with coconut (Cocos nucifera) roots was evaluated by next generation sequencing (NGS) using partial sequences of the 18S rDNA gene and by spore isolation and morphological identification from rhizosphere soil. Root samples from six different Green Dwarf coconut plantations and from one organic plantation surrounded by tropical dry forest along the coastal sand dunes in Yucatan, Mexico, were collected during the rainy and dry seasons. In total, 14 root samples were sequenced with the Illumina MiSeq platform. Additionally, soil samples from the dry season were collected to identify AMF glomerospores. Based on a 95-97% similarity, a total of 36 virtual taxa (VT) belonging to nine genera were identified including one new genus-like clade. Glomus was the most abundant genus, both in number of VT and sequences. The comparison of dry and rainy season samples revealed differences in the richness and composition of AMF communities colonizing coconut roots. Our study shows that the main AMF genera associated with coconut tree roots in all samples were Glomus, Sclerocystis, Rhizophagus, Redeckera, and Diversispora. Based on glomerospore morphology, 22 morphospecies were recorded among which 14 were identified to species. Sclerocystis sinuosa, Sclerocystis rubiformis, Glomus microaggregatum, and Acaulospora scrobiculata were dominant in field rhizosphere samples. This is the first assessment of the composition of AMF communities colonizing coconut roots in rainy and dry seasons. It is of importance for selection of AMF species to investigate for their potential application in sustainable agriculture of coconut.

Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrification

ABSTRACT: This study evaluated the effect of vitrification solutions and exposure time on the cryopreservation of Brazilian green dwarf coconut plumules (BGD) using the droplet vitrification technique. Explants were excised from BGD mature fruits from the Active Germplasm Bank of Embrapa Tabuleiros Costeiros, Sergipe, Brazil. Firstly, embryos were disinfected, and after excision, plumules were pre-cultivated for 72 hours in Y3 + 0.6 M sucrose + 2.2 g L-1 Gelrite® culture medium. Plumules were exposed to PVS2 and PVS3 solutions for 15 and 30 minutes and rapidly immersed in liquid nitrogen (-196 ºC). After cryopreservation, they were thawed in culture medium solution (Y3 + 1.2 M sucrose) and cultured in regeneration medium. The experimental design was completely randomized in a 2x2 factorial scheme (vitrification solutions per exposure times), with five replicates per treatment. Data were compared by the Tukey’s test at 5% probability. Significant differences were observed in the callogenesis percentage for the solutions x exposure time interaction for non-cryopreserved cultures (-NL) and for exposure time after cryopreservation (+NL). PVS2 and PVS3 combined with 15 minutes of exposure promoted the highest callus formation (70 and 100%, respectively) in control cultures. The exposure time of 30 min, regardless of vitrification solution, resulted in 30% embryogenic callus formation after cryopreservation. These results contributed to the long-term conservation of coconut palm.

Discovery of a novel CnAMADH2 allele associated with higher levels of 2-acetyl-1-pyrroline (2AP) in yellow dwarf coconut (Cocos nucifera L.)

Aromatic coconut is a special type of dwarf coconut containing a pleasant pandan-like aroma in its meat and water. 2-Acetyl-1-pyrroline (2AP) is a major constituent of the aroma in coconut and in other plants. A single nucleotide change in the coding sequence of CnAMADH2 has been associated with 2AP biosynthesis in aromatic ‘green’ dwarf coconut. However, this variant is not present in an aromatic ‘yellow’ dwarf coconut, suggesting that an alternative allele of amino aldehyde dehydrogenase 2 (CnAMADH2) is associated with the aroma in this variety. Here, we characterized CnAMADH2 in the aromatic yellow coconut to identify a novel aromatic allele. Compared with the aromatic green dwarf coconut and non-aromatic coconut, a single nucleotide change (G-to-A) was identified in exon 9 of CnAMADH2 in this yellow coconut causing an amino acid change (H299R). The H299R amino acid substitution resides at a highly conserved region of the ALDH cysteine (C) active site. According to the 3-D structural modeling results, this mutation could cause a reduction of aminoaldehyde dehydrogenase (AMADH) activity, thus increasing 2AP. This novel allele is likely associated with a higher level of 2AP. Functional markers specific to each of the two alleles of CnAMADH2 associated with the aroma trait in coconut were developed and successfully used to discriminate aromatic from non-aromatic coconut varieties. These markers would be useful for an aromatic coconut breeding program and for quality control of aromatic coconut production and cultivation.