You have accessJournal of UrologyStem Cell Research1 Apr 2016PD35-01 RENAL ARTERIAL INFUSION OF ADIPOSE TISSUE-DERIVED STROMAL VASCULAR FRACTION PROTECTS THE RENAL FUNCTION AGAINST ACUTE KIDNEY INJURY INDUCED BY ISCHEMIA-REPERFUSION INJURY Myoung Jin Jang, Bo Hyun Kim, Chunwoo Lee, Dalsan You, In Gab Jeong, and Chung-Soo Kim Myoung Jin JangMyoung Jin Jang More articles by this author , Bo Hyun KimBo Hyun Kim More articles by this author , Chunwoo LeeChunwoo Lee More articles by this author , Dalsan YouDalsan You More articles by this author , In Gab JeongIn Gab Jeong More articles by this author , and Chung-Soo KimChung-Soo Kim More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1073AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Acute kidney injury (AKI) induced by ischemia-reperfusion injury (IRI) is a major challenge in critical care medicine, with high rates of in-hospital morbidity and mortality. Preclinical studies suggested that administered cultured stem cells protected kidney function against AKI induced by IRI. Due to several concerns regarding the implantation of cultured stem cells into humans, however, recent clinical studies have focused on the use of stromal vascular fraction (SVF) in treating various diseases. The purpose of this study was to determine the therapeutic effects of adipose tissue-derived SVF and the optimal route for SVF delivery in a rat model of AKI induced by IRI. METHODS Fifty male Sprague-Dawley rats were randomly divided into 5 groups (10 animals per group): sham, nephrectomy control, IRI control, intra-renal arterial SVF infusion, and subcapsular SVF injection. To induce AKI induced by IRI, the left renal artery was clamped with a nontraumatic vascular clamp for 40 minutes, and the right kidney was removed. Serum creatinine and glomerular filtration rate were evaluated 1 day prior to IRI, and 2, 4, 7, 14 days after IRI. For histomorphometric studies, the kidney was removed 2 weeks after IRI. RESULTS Intra-renal arterial infusion of SVF significantly reduced the extent of elevation in serum creatinine compared with the IRI control group 4 days after IRI. The glomerular filtration rate of intra-renal arterial SVF infusion group was maintained at a level similar to those of the sham and nephrectomy control groups 14 days after IRI. Masson's trichrome stain for the degree of fibrosis showed that intra-renal arterial infusion of SVF was significantly less fibrosis than that observed in the IRI control group (P<0.001). Staining for alpha-smooth muscle actin showed that the number of small vessels was noticeably higher in the intra-renal arterial SVF infusion and subcapsular SVF injection groups than in the IRI control group (P<0.001). The number of terminal transferase-mediated dUTP nick-end labeling-positive cells increased after IRI, suggesting apoptosis or necrosis had occurred. Terminal transferase-mediated dUTP nick-end labeling assay showed significantly decreased apoptosis in both the intra-renal arterial SVF infusion and subcapsular SVF injection groups compared to the IRI control group (P<0.001). A greater increase in glutathione reductase and glutathione peroxidase was observed in both the intra-renal arterial SVF infusion and subcapsular SVF injection groups than in the IRI control group (P<0.001). These findings further suggest that anti-oxidative responses were elicited by IRI and SVF treatment contributed to further anti-inflammatory and anti-oxidative effects after IRI in this study. CONCLUSIONS Our study showed that renal function is effectively rescued from AKI induced by IRI through administration of SVF in a rat model and intra-renal arterial infusion of SVF was more effective than subcapsular injection. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e844 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Myoung Jin Jang More articles by this author Bo Hyun Kim More articles by this author Chunwoo Lee More articles by this author Dalsan You More articles by this author In Gab Jeong More articles by this author Chung-Soo Kim More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...