Background and aims Studies have shown an association between Neonatal hearing loss (NHL) and preterm birth and low birth weight, but the prevalence of NHL more specific within these categories is not known. Aim of this study is to present the risk of NHL by week of gestation and categories of birth weight in an nationwide cohort of neonates born Methods Results of the two-stage Automated Auditory Brainstem Response (AABR) Universal Newborn Hearing Screening Program in Dutch neonatal intensive care units and diagnostic examination were centrally registered between October 1998 and December 2012 and included in this study. NHL was defined as impaired when the neonates’ conventional Auditory Brainstem Response (ABR) level exceeded 35 dB in one (unilateral) or two (bilateral) ears at diagnostic examination. Birth weight was stratified into Results In total 18,564 neonates with a gestational age between 24.0 and 31.9 weeks were eligible for this study. Logistic regression analyses revealed significant associations between gestational age, birth weight and both unilateral and bilateral NHL (all p Conclusions Gestational age and birth weight are independently associated with NHL in a dose-response relationship. This information can be used to gain insight into health and related costs associated with very preterm births.