Pause Duration Research Articles

Single-Molecule Fluorescence Microscopy in Sensory Cilia of Living Caenorhabditis elegans.

Intracellular transport of organelles and biomolecules is vital for several cellular processes. Single-molecule fluorescence microscopy can illuminate molecular aspects of the dynamics of individual biomolecules that remain unresolved in ensemble experiments. For example, studying single-molecule trajectories of moving biomolecules can reveal motility properties such as velocity, diffusivity, location and duration of pauses, etc. We use single-molecule imaging to study the dynamics of microtubule-based motor proteins and their cargo in the primary cilia of living C. elegans. To this end, we employ standard fluorescent proteins, an epi-illuminated, widefield fluorescence microscope, and primarily open-source software. This chapter describes the setup we use, the preparation of samples, a protocol for single-molecule imaging in primary cilia of C. elegans, and data analysis.

Infectious complications in pediatric traumatology and orthopedics (analysis of clinical series)

BACKGROUND: Infectious complications in pediatric traumatology and orthopedics are relatively rare. Publications on this issue are less common than in the adult population. However, the resulting complications often lead to a persistent anatomical and functional defect, bearing the economic burden and legal consequences.
 AIM: To analyze a clinical series of pediatric patients with infectious complications of injuries and surgical interventions in the skeletal system.
 MATERIALS AND METHODS: A retrospective analysis of the clinical series of patients over 5 years was conducted. The study involved children aged 18 years. A sample of 34 children was included. The average age was 13.5 (minmax, 417 years). Patients with infectious and inflammatory complications after mechanical and/or surgical trauma, availability of a complete data archive (anamnesis, radiation therapy, and medical documentation), traced relief of inflammatory phenomena, and achievement of remission of the chronic process for more than 6 months were included.
 RESULTS: Infectious complications of injuries were noted in 26 (76%) patients, complications of orthopedic interventions in 8 (24%), and open injuries in 9 (34%). The injuries were isolated in 11 (42%) children, multiple trauma in 5 (19%), and combined in 10 (39%) patients. Defects of large skeletal segments were common: the thigh in 8 (24%) patients, humerus in 5 (15%), shin in 5 (15%), and spine in 4 (12%). Five (15%) had infection of the pelvic bones, and one patient had infectious complications in the lower jaw, collarbone, elbow joint, knee joint arthritis, and foot bones. Superficial infection of the surgical intervention area was noted in 3 cases (9%), deep infection in 27 (79%), and posttraumatic osteomyelitis in 4 (12%). The average duration of the diagnostic pause was 33 days, and the therapeutic pause was 36 days. Antibacterial prophylaxis before surgery for closed injuries and orthopedic operations was carried out in only 17 patients. Bacteriological verification was not performed in 8 (23.5%) patients with complications. In 9 (26%) patients, it was not possible to identify a microbial agent in the presence of a clinical picture. The structure of pathogens correlates with the literature data on the problem in the adult population. In 10 out of 25 positive bacteriological studies, polyresistant strains were isolated. Thirty-three children underwent surgery, and the basic principle is radical surgical rehabilitation. The average number of interventions performed was 3 (minmax, 112). Stable relief of the infectious and inflammatory process has been achieved in all cases. The average period of inpatient treatment was 39 days, recovery was achieved in 24 (71%) children, and persistent anatomical and functional defect and disability were noted in 10 (29%) children.
 CONCLUSIONS: Infectious complications in pediatric traumatology should be considered a complex multidisciplinary problem, and part of the solutions largely lies in the field of healthcare organization, specifically with an adequate choice of indications, compliance with the osteosynthesis technique, compliance with the principles of antibiotic prophylaxis, patient routine, and training of pediatric surgeons and orthopedic traumatologists on surgical infections, using modern principles and technologies for the treatment of these complications.

USING RHYTHM-FORMING EXERCISES TO ELIMINATE STUTTERING

The article discusses the issue of psychological andpedagogical support and assistance to children with stuttering, one of the most severe speech defects. The recommended exercises for eliminating stuttering consist in mastering and developing a rhythmic pattern, mastering the ratio of the duration of rhythmic work and pauses.

Does language matter in oral diadochokinesis (DDK) performances?

Oral diadochokinesis (DDK) typically refers to rapid repetitions of monosyllables (e.g., /pʌ/, /tʌ/, /kʌ/) or multisyllables (e.g., /pʌtʌkʌ/). DDK has been frequently included in studies of motor development and disorders and its use has increased over the past decade. One unanswered question in using DDK for investigation of speech motor control is whether the studied language serves as an important factor in DDK performances. Despite the general agreement that DDK performance (mostly DDK rates) is little affected by a speaker’s native language, few data exist on language- or dialect-specific effects on DDK, which may be explained by factors such as speaking rate and rhythmic pattern of the languages. In this study, we compare DDK performances in two languages, American English (AE) and Korean, which were chosen primarily because of the difference in their rhythmic patterns (stressed-timed versus syllable-timed). A total of 80 young adults (40 AE-, 40 Korean-speakers) performed both mono- and multi-syllable repetitions and analysis was limited to the first 5 seconds of each performance. We will present data on several aspects of DDK performance focusing on prosodic patterns (syllable and intersyllable pause durations, intensity, and F0 across syllables) in addition to average DDK rates.

Simulation of autonomous voltage inverters of power sources of single-phase asynchronous drives

Purpose. Development of original models of voltage inverters adapted to control a single-phase asynchronous motor with the subsequent prospect of creating automatic systems for improving the energy efficiency of household mechanisms, as well as developing educational complexes for studying the operation of frequency converters to form skills for their repair and tuning.Methodology. The provisions of the theory of electric machines, methods of synthesis of digital control systems, methods and methods of modeling in CAD electronic laboratory of the company NATIONAL INSTRUMENTS of the Multisim component, methods of circuit design were used for conducting research. Findings. Models of pulse distributors were developed and built for the cases of power supply of single-phase asynchronous motors with bridge inverters of separate and individual starting and working windings, half-bridge inverters with a bipolar power source and a three-phase inverter with control according to a 180-degree control diagram. Time diagrams of visual confirmation of the system's performance according to the declared principles have been obtained. Originality. A number of original switch diagrams of the power section of the models of impulse distributors of autonomous voltage inverters are proposed, taking into account the phase shift and the duration of the switching pauses of the switching arms of the power unit to avoid emergency modes, recommendations are given on the levels of generation frequencies that are fed to the input of the combinational circuit, the prospect of using this system as part of a single-phase asynchronous electric drive with the possibility of energy-efficient control is shown.Practical value. The proposed original model solutions for autonomous voltage inverters, which are adapted to work together with a single-phase IM, can be useful for developers of converter technology and become visual material for their use in the educational process for training specialists in specialty 141 "Electric power engineering, electrical engineering and electromechanics".

Design of Acoustic Signal for Positioning of Smart Devices.

This paper addresses the limitations of using smartphones in innovative localization systems based on audio signal processing, particularly in the frequency range of 18-22 kHz, due to the lack of technical specifications and noise characterization. We present a comprehensive study on signal design and performance analysis for acoustic communication in air ducts, focusing on signal propagation in indoor environments considering room acoustics and signal behavior. The research aims to determine optimal parameters, including the frequency band, signal types, signal length, pause duration, and sampling frequency, for the efficient transmission and reception of acoustic signals for commercial off-the-shelf (COST) devices. Factors like inter-symbol interference (ISI) and multiple access interference (MAI) that affect signal detection accuracy are considered. The measurements help define the frequency spectrum for common devices like smartphones, speakers, and sound cards. We propose a custom signal with specific properties and reasons for their selection, setting the signal length at 50 ms and a pause time of 5 ms to minimize overlap and interference between consecutive signals. The sampling rate is fixed at 48 kHz to maintain the required resolution for distinguishing individual signals in correlation-based signal processing.

Effect of clear speech on acoustic measures of dysprosody in Parkinson disease for different reading tasks

Purpose The purpose of the study was to determine the effect of clear speech instruction on acoustic measures of dysprosody between reading passages of differing linguistic content for speakers with and without Parkinson Disease (PD). Method Ten speakers with PD and 10 controls served as participants and read five simple and three standard reading stimuli twice. First, speakers read habitually and then following clear speech instruction. Acoustic measures of fundamental frequency variation (semitone standard deviation, STSD), articulation rate, and between-complex pause durations were calculated. Result Results indicated speakers with PD exhibited less fundamental frequency variation than controls across reading stimuli and instructions. All speakers exhibited lower STSD and longer between-complex pause durations for the standard compared to simple reading stimuli. For clear speech, all speakers reduced articulation rate and increased between-complex pause durations in both simple and standard reading stimuli. However, speakers with PD exhibited a significantly less robust reduction in articulation rate for clear speech than control speakers for all reading stimuli. Conclusion Linguistic content of reading stimuli contributes to differences in fundamental frequency variation and pause duration for all speakers. All speakers reduced articulation rate for clear speech compared to habitual instruction, but speakers with PD did so to a lesser extent than controls. Linguistic content of reading stimuli to examine dysprosody in PD should be considered for clinical application.

Slow Interstitial Fluid Flow Activates TGF-β Signaling and Drives Fibrotic Responses in Human Tenon Fibroblasts.

Fibrosis limits the success of filtering glaucoma surgery. We employed 2D and 3D in vitro models to assess the effects of fluid flow on human tenon fibroblasts (HTF). HTF were exposed to continuous or pulsatile fluid flow for 48 or 72 h, at rates expected at the transscleral outflow site after filtering surgery. In the 2D model, the F-actin cytoskeleton and fibronectin 1 (FN1) were visualized by confocal immunofluorescence microscopy. In the 3D model, mRNA and whole cell lysates were extracted to analyze the expression of fibrosis-associated genes by qPCR and Western blot. The effects of a small-molecule inhibitor of the TGF-β receptor ALK5 were studied. Slow, continuous fluid flow induced fibrotic responses in the 2D and 3D models. It elicited changes in cell shape, the F-actin cytoskeleton, the deposition of FN1 and activated the intracellular TGF-β signaling pathway to induce expression of fibrosis-related genes, such as CTGF, FN1 and COL1A1. ALK5-inhibition reduced this effect. Intermittent fluid flow also induced fibrotic changes, which decreased with increasing pause duration. Slow interstitial fluid flow is sufficient to induce fibrosis, could underlie the intractable nature of fibrosis following filtering glaucoma surgery and might be a target for antifibrotic therapy.

Global Acoustic Speech Temporal Characteristics for Mandarin Speakers With Parkinson's Disease During Syllable Repetition and Passage Reading.

Previous research has revealed considerable variation in speech rates among English speakers with Parkinson's disease (PD) with slower, faster, or similar rates than controls. The purpose of this study was to characterize speech rates of Mandarin speakers with PD and the corresponding articulation and pause characteristics explaining the speech rates to enhance rate control therapies. Eighteen Mandarin speakers with PD and 18 controls produced syllable repetitions and passage reading using their typical speech style. Speech rates, articulation rates, mean pause durations (≥ 10 ms), and the number of pauses with duration between 10 ms and 300 ms and greater than 300 ms were measured and compared between groups and tasks as well as across the initial, middle, and final periods of the passage. Two-way, mixed-model analyses of variance were employed for data analysis. Compared to controls, individuals with PD spoke with similar speech rates and faster articulation rates during passage reading, whereas during syllable repetitions, they produced slower speech rates and comparable articulation rates. The slower syllable repetitions produced by speakers with PD may be explained by longer pauses and more perceptual pauses, whereas faster articulation rates may explain the trend of faster speech rates during reading. Speech and articulation rates accelerated for both groups during passage reading. Speech rates of Mandarin speakers with PD were characterized by faster articulation, longer pauses, and more perceptual pauses for passage reading. A descriptive model of speech rate suggested that speakers with PD and dysarthria in this study would benefit from rate reduction therapy decreasing articulation rate. https://doi.org/10.23641/asha.23982282.

Pausing and the ‘Othello Error’

The idea of detecting deception from speech is very attractive from a law enforcement perspective, yet research considering the possibility has yielded conflicting results, due to the practical difficulties in investigating the topic. Scientific research is yet to provide forensic linguistics with a reliable means of discerning lies from truths. The present study explores the relationship between truthfulness and pausing behaviour. Various aspects of the acoustics of pausing behaviour were investigated for Standard Southern British English in 30 mock police interviews from the DyViS database (Nolan et al. 2009). A novel distinction was made between prescribed and unprescribed lies, to delineate a potential source of differences in the unscripted content of speakers’ untruthful responses. Among pause duration measures, statistically significant differences were found across all three response types (truth, prescribed lie, unprescribed lie) for response latency, between truths and lies for initial filled pauses, and between unprescribed lies and the other response types for silent pauses. For pause frequency measures, only internal filled pauses showed a statistically significant difference: truths differed from both types of lies, but prescribed lies did not differ from unprescribed lies. Theories of cognitive effort and attempted control are drawn on in accounting for these findings.

Modified subtotal laryngectomy with formation of a tracheopharyngeal shunt for locally advanced cancer of the larynx and hypopharynx

Aim. To study the effectiveness of modified subtotal laryngectomy with the formation of a tracheopharyngeal shunt in patients with locally advanced cancer of the larynx and laryngopharynx.Materials and methods. We conducted a cohort study on the use of combined treatment (surgical and radiation/ polychemotherapy) in 25 patients: 24 men, 1 woman; median age – 56 years. The subtotal laryngectomy technique that we developed and used included the preservation of one arytenoid cartilage, the posterior third of the vocal fold, and the recurrent nerve on the side not affected by the tumor. We studied postoperative complications, the length and diameter of the tracheopharyngeal shunt, the functioning of the arytenoid cartilage, the frequency of voice recovery and its quality, and overall and relapse-free survival.Results. In the postoperative period, 8 (32%) patients developed a pharyngocutaneous fistula; tracheal stenosis after tracheostomy was observed in 8 (32%) cases. The length of the tracheopharyngeal shunt varied from 16.4 to 25.6 mm, in all cases its course was tortuous, the diameter of the preserved part of the vocal fold varied from 1.1 to 1.5 mm. 24 (96%) patients demonstrated the functioning of the arytenoid cartilage and its work as a valve. Voice function recovered in 24 (96%) patients. Fundamental frequency was 155.3 ± 20.9 Hz, maximum phonation time of the vowel “A” – 3.5 ± 1.0 s, duration of pauses in phrase – 0.24 ± 0.03 s, speech rate – 99.8 ± 8.4 words/ min, number of words in phrase 6.5 ± 0.3. The average value of the Acoustic Voice Quality Index was 6.74, which indicates dysphonia (for the Russian language, the threshold value for dysphonia is 4.86), but this did not interfere with patients’ communication at home and on the phone. Overall and relapse-free survival at 1, 2, and 3 years were 100%, 89%, 78% and 86%, 78%, 72%, respectively.Conclusion. The method of operation proposed by us contributes to the restoration of voice function in the majority of patients with oncological outcomes comparable to total laryngectomy.

Speed of Information Processing and Verbal Working Memory in Children and Adolescents With Cochlear Implants.

Verbal working memory delays are found in many deaf children with cochlear implants compared with normal-hearing peers, but the factors contributing to these delays are not well understood. This study investigated differences between cochlear implant users and normal-hearing peers in memory scanning speed during a challenging verbal working memory task. To better understand variability in verbal working memory capacity within each sample, associations between memory scanning speed, speech recognition, and language were also investigated. Twenty-five prelingually deaf, early implanted children (age, 8-17 yr) with cochlear implants and 25 normal-hearing peers completed the Wechsler Intelligence Scale for Children, Fifth Edition, Letter-Number Sequencing (LNS) working memory task. Timing measures were made for response latency and average pause duration between letters/numbers recalled during the task. Participants also completed measures of speech recognition, vocabulary, and language comprehension. Children with cochlear implants had longer pause durations than normal-hearing peers during three-span LNS sequences, but the groups did not differ in response latencies or in pause durations during two-span LNS sequences. In the sample of cochlear implant users, poorer speech recognition was correlated with longer pause durations during two-span sequences, whereas poorer vocabulary and weaker language comprehension were correlated with longer response latencies during two-span sequences. Response latencies and pause durations were unrelated to language in the normal-hearing sample. Children with cochlear implants have slower verbal working memory scanning speed than children with normal hearing. More robust phonological-lexical representations of language in memory may facilitate faster memory scanning speed and better working memory in cochlear implant users.

Respiratory-Swallow Coordination in Individuals with Parkinson's Disease: A Systematic Review and Meta-Analysis.

Swallowing impairment, including altered physiology and aspiration, occur across the progression of Parkinson's disease (PD). The phase of respiration during which a swallow is initiated has been linked to swallowing impairment and aspiration in cohorts with dysphagia following stroke and head and neck cancer treatment, but has been understudied in PD. If similar findings are shown in individuals with PD, the implications for swallowing assessment and treatment are significant. The aim of this systematic review and meta-analysis of literature was to examine respiratory-swallow coordination measures and potential implications on swallowing physiology in individuals with PD. An extensive search of 7 databases (PubMed, EMBASE, Central, Web of Science, ProQuest Dissertations & Theses, Scopus, and CINAHL) with predetermined search terms was conducted. Inclusion criteria were individuals with PD and the use of objective evaluations of respiratory-swallow coordination. Of the 13,760 articles identified, 11 met the inclusion criteria. This review supports the presence of atypical respiratory swallow patterning, respiratory pause duration and lung volume at swallow initiation in individuals with PD. The meta-analysis estimated an occurrence of 60% of non-expiration-expiration and 40% of expiration-expiration respiratory phase patterns surrounding swallowing. Although this systematic review supports the occurrence of atypical respiratory-swallow coordination in individuals with PD, the evidence is limited by the variability in the methods of data acquisition, analysis, and reporting. Future research examining the impact of respiratory swallow coordination on swallowing impairment and airway protection using consistent, comparable, and reproducible methods and metrics in individuals with PD is warranted.

Using voice biomarkers for frailty classification.

Clinicians use the patient's voice intuitively to evaluate general health and frailty. Voice is an emerging health indicator but has been scarcely studied in the context of frailty. This study explored voice parameters as possible predictors of frailty in older adults. Fifty-three participants over 70years old were recruited from rehabilitation wards at a tertiary medical center. Participants' frailty was assessed using Rockwood frailty index and they were classified as most-frail (n = 33, 68%) or less-frail (n = 20, 32%). Participants were recorded counting from 1 to 10 and backwards using a smartphone recording application. The following voice biomarkers were derived: peak and average volume, peak/average volume ratio, pauses' total length, and pause length standard deviation. The most-frail group had a higher peak volume/average volume ratio (p = 0.03) and greater variance in lengths of pauses between speech segments (p = 0.002). These parameters indicate greater speech irregularity in the most-frail, compared to the less-frail. The most-frail group also had a longer total duration of pauses (p = 0.02). No statistically significant difference was found in peak and average volume (p = 0.75 and 0.39). Most-frail participants' speech had different characteristics, compared to participants in the less-frail group. This is a first step to developing an AI-based frailty assessment tool that can assist in identifying our most vulnerable patients.

Effects of speaking task and proficiency on the midclause pausing characteristics of L1 and L2 speech from the same speakers

AbstractThis study explored the effect of speaking task on midclause pausing characteristics in the L1 and L2 speech of the same speakers to gain further insights into the potential relationship between pause location and stages of speech production. Participants included English L1 learners of L2 French (n = 29) or Spanish (n = 27) from the publicly available, longitudinal LANGSNAP corpus. Participants completed two oral tasks in their L1 and L2: a picture-based narrative and a semistructured interview. The rate, duration, and proportion of midclause pauses were compared between tasks in the L1 as well as in the L2 before and during residence abroad. In the L1, results indicated more fluent performance in the narrative task except for rate. When speaking in their L2, participants showed improvement on each measure in the narrative task but ultimately remained less fluent in their L2 in comparison to their L1. In the interview task, the only measure of midclause pausing that consistently differentiated L1 from L2 speech was midclause pause rate. The findings call for a nuanced interpretation of connections between midclause pausing and formulation and suggest that midclause pause rate is least influenced by speaking task.

Reduced duration of stuttering-like disfluencies and consistent anticipatory slowing during an adaptation task.

The adaptation effect in stuttering, traditionally described as the reduction of stuttering moments over repeated readings, provides a context to investigate fluency facilitation as well as a relatively controlled means of comparing fluent speech in the immediate vicinity of words that were stuttered versus fluently produced. Acoustic studies have documented decreased duration of fluent speech during adaptation but rarely address changes in disfluencies or the speech preceding or following the disfluencies. This study addresses this gap in the research by documenting frequency and duration changes in both fluent and stuttered syllables. Fifteen people who stutter read passages aloud five times in succession. Frequency and duration of fluent syllables, pauses, stuttering-like disfluencies (SLDs) and other disfluencies (ODs) were compared across the five readings. In addition, durations for syllables before and after pauses and SLDs were compared to determine if there were anticipation or carryover effects of SLDs on surrounding syllables. Durations measured for more than 22000 fluent syllables, 1531 pauses, 128 ODs and 1752 SLDs. For most of the 15 participants, significant decreases in both frequency and duration of SLDs over the five readings were observed. In addition, lengthening of fluent syllables immediately preceding the disfluent syllables was observed: this pre-SLD lengthening did not change over the five readings. Decreased duration of SLDs across readings supports the motor practice hypothesis, which assumes that successive reading of the same text increases the efficiency of the speech motor plans resulting in less stuttering and decreased durations of the stuttering that persists. Pre-SLD lengthening merits further study, because it informs our knowledge of the time course of stuttered events and may be associated with conscious or unconscious anticipation of upcoming SLDs that does not decrease with motor practice. What is already known on this subject The frequency of stuttering-like disfluencies (SLDs) can be reduced using a variety of fluency-enhancing strategies. For example, the adaptation effect, in which a reduction of stuttered events occurs over repeated readings of the same material, has been widely studied. Previous studies have shown that durations of fluent syllables decrease during adaptation, supporting the hypothesis that repeated practice of the motor plan leads to increased fluency. However, temporal changes in disfluent syllables and syllables preceding and following SLDs have rarely been studied, so our understanding of the effect of motor practice on stuttering reduction is incomplete. What this study adds This study has two significant findings. First, stuttered disfluencies that persisted after the initial reading of the adaptation task tended to become shorter in duration. Fluently produced syllables and those that were stuttered, both of which are speech events related to motor control of articulators, were affected in a similar manner by the motor practice associated with adaptation. Second, lengthening of fluent syllables immediately preceding stuttered syllables was observed. This pre-stuttering lengthening, however, did not decrease in duration over the five readings: the mechanism that drives this anticipatory behaviour is not affected by repeated practice. What are the clinical implications of this work? People who stutter have neural differences that lead to speech motor planning and/or execution that is less efficient than that of typical speakers. The finding that stuttering is reduced and that persisting SLDs become shorter in duration over repeated readings provides evidence that motor practice can influence the manifestation of stuttering by temporarily making those specific motor plans more efficient. This may inform treatments for stuttering. The observation that fluent syllables immediately before SLDs were lengthened, and that this lengthening was not influenced by repeated practice, extends our understanding of the time course of stuttering events and may be useful in understanding anticipation and listener reactions to stuttering.

Relation between the phonation time in staccato samples and the method of performing selected language tasks in Polish: research conclusions

This article presents a discussion on the results of the research on the staccato phonation conducted as part of the pilot study intended to establish phonation time norms in Polish-speaking adults. The analysed data were excerpted from a selected group of 12 women. Statistical analyses served the purpose of identifying potential differences between the results of tasks concerning the staccato phonation of selected Polish sounds, the staccato phonation and the legato phonation, and determining the correlation between staccato and other language tasks. The interpretation of the results of the research as proposed in the text refers to the observations regarding the task performance method, e.g. differences in the duration of pauses in the language tasks compared to the staccato phonation tasks, diversities related to the sound realisation time and method in the language tasks and in the staccato phonation task. Keywords: Maximum Phonation Time (MPT) – staccato – legato – norm – speech-language pathology.

Vocal Emotional Expression in Parkinson’s Disease: Roles of Sex and Emotions

Introduction: Parkinson’s disease (PD) commonly causes speech impairments, including difficulties in expressing emotions through voice. Method: The objective of this study was to investigate gendered vocal expressions of fear, anger, sadness, and happiness for mild to moderate PD. Prosodic features (related to fundamental frequency (F0), intensity (I), speech rate, articulation rate, and number and duration of pauses) and acoustic correlates of voice quality (CPPS, jitter, shimmer, and HNR) were collected from 14 patients with PD (mean age = 69.93; SD = 7.12; 8 males, 6 females) and 13 healthy controls (HC) (mean age = 68.13; SD = 8.27; 5 males, 8 females) matched for age, sex, and years of education. The utterances were extracted from four emotional and one neutral text. The neutral utterance and the emotional utterances were compared. Intra-sex comparison (female with PD vs. female HC and male with PD vs. male HC) and inter-sex comparison (female vs. male both for patients with PD and for HC), were performed with the Mann–Whitney test. A Mann–Whitney test was also used to compare the different emotional conditions, considering sex and PD diagnosis as well. Results: No significant intra-sex differences were found for the neutral speech, but inter-sex differences emerged. Regarding emotional speech, females with PD featured lower MaxF0 than female HCs for happiness and higher intensity variability (SD I) for sadness. Utterances by females with PD had lower CPPS than utterances by HCs for anger and fear. Utterances by males with PD had lower minimum intensity (MinI) than utterances by male HCs when expressing fear. Conclusions: Emotional vocal expression in individuals with PD was found to be impaired and showed sex differences. These findings have the potential to significantly impact the quality of life of PD patients.

Pausing and fluency in speech of patients with relapsing-remitting multiple sclerosis

ABSTRACT Multiple Sclerosis (MS) causes a variety of symptoms in speech production, such as more frequent pauses and an increase in the duration of pauses in the speech. However, there is almost no data on whether the disease affects speech fluency in other ways, such as changes in the frequency of disfluencies in speech. The main question of this study is the following: if we examine speech fluency in speech tasks requiring different cognitive load, will there be a difference between patients and controls? Twenty people with relapsing-remitting MS (3 men and 17 women) and 20 age- and education-matched control speakers (4 men and 16 women) participated in the study. Speech samples were recorded with each participant in three speech tasks: 1) spontaneous narratives about their own lives, 2) narratives about their previous day, and 3) narrative recalls based on a heard text. In the speech samples, pauses and disfluencies were annotated and the duration of pauses was measured. Then, the frequency of pauses and disfluencies were calculated and the types of disfluencies were examined. The results show that there are differences in the frequency and duration of pauses between people with MS and controls. However, there were no significant differences in the frequency of disfluencies between the groups. The same types of disfluencies occurred in the same frequency in both groups. The results help to better understand the speech production processes in MS.

Speech timing and monosyllabic diadochokinesis measures in the assessment of amyotrophic lateral sclerosis in Canadian French

Purpose The primary objective of this study was to determine if speech and pause measures obtained using a passage reading task and timing measures from a monosyllabic diadochokinesis (DDK) task differ across speakers of Canadian French diagnosed with amyotrophic lateral sclerosis (ALS) presenting with and without bulbar symptoms, and healthy controls. The secondary objective was to determine if these measures can reflect the severity of bulbar symptoms. Method A total of 29 Canadian French speakers with ALS (classified as bulbar symptomatic [n = 14] or pre-symptomatic [n = 15]) and 17 age-matched healthy controls completed a passage reading task and a monosyllabic DDK task (/pa/ and /ta/), for up to three follow-up visits. Measures of speaking rate, total duration, speech duration, and pause events were extracted from the passage reading recordings using a semi-automated speech and pause analysis procedure. Manual analysis of DDK recordings provided measures of DDK rate and variability. Result Group comparisons revealed significant differences (p = < .05) between the symptomatic group and the pre-symptomatic and control groups for all passage measures and DDK rates. Only the DDK rate in /ta/ differentiated the pre-symptomatic and control groups. Repeated measures correlations revealed moderate correlations (rrm = > 0.40; p = < 0.05) between passage measures of total duration, speaking rate, speech duration, and number of pauses, and ALSFRS-R total and bulbar scores, as well as between DDK rate and ALSFRS-R total score. Conclusion Speech and pause measures in passage and timing measures in monosyllabic DDK tasks might be suitable for monitoring bulbar functional symptoms in French speakers with ALS, but more work is required to identify which measures are sensitive to the earliest stages of the disease.