Publisher Summary The early Drosophila embryo is a useful system for functional studies of the centrosome. This organelle plays essential and diverse roles in guiding nuclear movement and cytoskeletal reorganization during the initial 3 hours of development. Drosophila embryogenesis begins with 13 synchronous nuclear divisions that occur without accompanying cytokinesis. The divisions immediately following fertilization proceed as an expanding ball in the interior of the embryo. The centrosome-based activities coordinate nuclear and cytoskeletal dynamics during these divisions. Because Drosophila is amenable to molecular genetic, biochemical, and a variety of cellular approaches, the mechanisms underlying many activities of the centrosome in the early embryo are addressed. This chapter discusses the mechanisms that control centrosome movement and migration, the role of the centrosome in coordinating nuclear and cytoskeletal events of the cell cycle, and how the centrosome directs the dramatic microfilament and plasma membrane rearrangements at the cortex. The mechanism of centrosome duplication and its regulation and the issues of ultrastructure, composition, duplication, separation, fertilization, mitotic, and precortial division are described and provide insights into centrosome function.
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