Duplicate Diet Research Articles

Human Exposure through the Diet to Arsenic and Other Toxic Elements: A Literature Review of Scientific Studies Conducted in Catalonia, Spain, in the Current Century.

Human exposure to arsenic and other toxic elements such as cadmium, lead and mercury may lead to a wide range of adverse health effects. In relation to this, it is well established that the diet is the main route of exposure to both essential and toxic trace elements. In recent years, the levels of toxic elements in foodstuffs have been measured in numerous studies conducted all over the world. Scientific databases show that, in the current century, China and Spain have been the countries where the most surveys on this topic have been carried out. Regarding Spain, Catalonia is the region where most studies aimed at determining the concentrations of trace elements in food have been performed. The objective of this paper was to review the studies carried out in Catalonia on the concentrations of As and toxic metals (including Cd, Hg and Pb) in food, as well as their estimated dietary intakes (EDIs). The results of total diet studies (TDSs) and duplicate diet (DD) studies have been included. For most toxic elements, a continued reduction in the EDI has been observed. This reduction is associated with a decrease in their concentrations in food, and with certain changes in dietary habits. Fish and seafood is the food group showing the highest content of toxic elements. However, none of the adult groups exceeded-in general-the safety thresholds for As, Cd, Hg and Pb established by the European Food Safety Agency (EFSA).

Intake and excretion of cadmium of Japanese adult

ObjectiveIt is the aim of this study if the recent Cd intake and excretion is consistent with the regression equation proposed by Ikeda et al. (Environ. Health Prev. Med. 20 (2015) 455–459) which utilized intake/excretion data of 3 decades ago MethodMatched duplicate diet and spot urine samples were collected in 2017–2019 from 150 Japanese adults (85 females and 65 males, mean age being 44.9) for the estimation of daily Cd intake and urinary excretion. Cd concentration in diet and urine was measured by ICP mass spectrometry. ResultGeometric mean (geometric standard deviation) of Cd intake was 12.1 (1.67) μg/person/day or 0.206 (1.66) μg/kg body wt./day. Urinary Cd concentration was 0.54 (2.6) μg/g creatinine or 0.67 μg/L (2.3) (gravimetric correction). These were lower than those reported previously for Japanese populations. Urinary concentration was significantly higher in females than in males for both creatinine and gravimetric corrections. Dietary intake was higher in males than in females but this difference diminished after body weight correction. There was no significant correlation between Cd intake and urinary concentration on individual basis, which was expected because urinary Cd concentration reflects Cd body burden but not recent intake level. Meanwhile, the geometric mean intake and urine concentration was consistent with the regression equation proposed by Ikeda et al., which was based on intake and excretion surveys for 30 non-exposed Japanese female populations. ConclusionThis result supported that the regression equation of Ikeda et al. could be used for conversion of urinary Cd excretion level of a population to intake level.

Dietary intake of methylmercury by 0-5 years children using the duplicate diet method in Japan.

The developing brains are sensitive to methylmercury (MeHg). However, the exposure to MeHg in baby foods and toddler meals remains unknown. This study aimed to determine MeHg intake from baby food or toddler meals, and to investigate the relationship with child hair total mercury (THg). A total of 3 days of 24-hour dietary diet and hair samples were collected from 260 consenting children aged 0-5 years. We measured the concentrations of THg and MeHg in the diet and THg in the hair. The results of measuring THg were below both the method detection and method quantification limits or either of both in powdered milk (93.8%), 5-6 months (53.3%), and 7-8 months (39.5%). The median daily THg intake was 20.3 (95% confidence interval 0.72-232.5) ng/kgbw. MeHg was not detected in 213 samples with dietary THg concentrations below 1 ng/g. The MeHg concentration with THg concentrations of 1 ng/g or higher was 1.70 (0.87-6.21) ng/g, and MeHg percentage in THg was 90.0%. To estimate MeHg intake, we multiplied the THg concentration by 90.0%, resulting in an estimated MeHg intake of 18.3 (0.65-209.2) ng/kgbw/day. The THg in children's hair was 1.05 (0.31-3.96) ppm, and a weak positive correlation was observed between hair THg and dietary MeHg (r = 0.170). This study highlights the accurate estimation of MeHg intake in children using a duplicate method. Japanese children consume fish, the MeHg intakes exceeded the reference dose and/or provisional tolerable weekly intake in several children. Further discussion based on epidemiological data is required.

Dietary Exposure to Acrylamide in Spanish University Canteens by the Duplicate Diet Method.

During the university period, many students adopt new dietary patterns, sometimes including the excessive consumption of highly processed foods, which can expose them to process contaminants such as acrylamide. This research aimed to evaluate the dietary exposure to acrylamide of Spanish university students in their campus canteens using the duplicate diet method, and to estimate the associated health risks based on their food consumption habits. Apart from potato-based foods, the analysed food/meals contained non-detectable or low levels of acrylamide (<63 µg/kg). Two exposure scenarios were considered, depending on whether students selected salad (lowest exposure) or processed potatoes (highest exposure) as side dishes. The frequent consumption of processed potatoes could increase acrylamide intake from 6.5 to 26.4 µg/day. Due to their lower body weight, women had a higher risk of acrylamide exposure in both scenarios. The margin of exposure (MOE) values for neoplastic effects indicated high levels of health concern, even only considering the main meal of the day. Furthermore, the risk of acrylamide exposure could increase by more than four times depending on the side dish selected by the students. This research highlights the need to promote healthy dietary habits among young people, encouraging the selection of safer food options in terms of food processing contaminants.

A human health risk assessment of rare earth elements through daily diet consumption from Bayan Obo Mining Area, China

Rare earth elements (REEs) have been broad application in a range of industries, including the electronics industry, advanced materials, and medicine. However, health risks associated with REEs received increasing attention. 31 residents (16 males and 15 females) from Bayan Obo mining in Inner Mongolia, China, were enrolled in this study. In total, 677 food samples, the major human exposure matrices (drinking water and duplicate diets), and bio-samples (urine and blood) of 31 participants were obtained. The concentrations of REEs were measured to characterize their external and internal exposures, and the potential health risk of exposure to REE through the ingestion route was analyzed. The results revealed that the detection rate in blood samples (100%) is higher than in urine (32.86%), and only a few REEs were detected in water samples (8.06%), the urine concentrations were considerably lower than in blood. Exposure to REEs through drinking water was considered negligible compared to food intake. Lanthanum and cerium were the most concentrated REEs in food samples. Health risks were calculated based on a dose-response model, the total hazard quotients (THQ) values for all food groups were within normal levels, and the Monte Carlo simulation results show that the 5th, the 50th, and the 95th percentile values of HI were found as 1.45 × 10−2, 3.52 × 10−2, and 9.13 × 10−2, respectively, neither exceeds the threshold, indicating low health risks associated with food intake exposure for this area. The sensitivity results suggest that underweight people are at higher risk, cerium, lanthanum, and yttrium concentrations, and food intake contributes more to health risks. The use of probability distribution methods can improve the accuracy of the results. The cumulative health risk through food intake is negligible, and further attention should be paid to the health risk induced by other routes of exposure to REEs by the local residents.

Assessment of dietary acrylamide exposure in children attending Spanish school canteens using the duplicate diet method

Acrylamide is a carcinogenic chemical contaminant formed in heat-treated foods. In this study, a duplicate diet method was used to evaluate the acrylamide content of foods/meals served at breakfast and lunch in two Spanish school canteens. The dietary acrylamide intake in students was estimated within lower bound (LB) and upper bound (UB) scenarios. Biscuits exhibited the highest acrylamide values, exceeding the benchmark level established by the European Regulation 2017/2158 (350 μg/kg), followed by main courses such as stews, side dishes and bread. In the LB scenario, breakfasts accounted for the major contributors to the daily acrylamide intake (73.3%). However, lunches were the main responsible in the UB scenario (65.4%). Acrylamide exposure was estimated for three age ranges: 3-5y pre-school children (0.59 and 0.92 μg/kg body weight/day), 6-9y children (0.43 and 0.67 μg/kg body weight/day) and 10-12y early adolescents (0.28 and 0.44 μg/kg body weight/day). Margins of exposure for neoplastic effects ranged between 144 and 1026, which is below the reference of 10,000, indicating a health concern. The findings reveal that any diet, even one that is balanced, varied and contains foods low in acrylamide, involves an additive exposure to the contaminant that should be considered when conducting acrylamide exposure risk assessments.

Assessment of exposure to pesticides: residues in 24 h duplicate diets versus their metabolites in 24 h urine using suspect screening and target analysis

Human biomonitoring can add value to chemical risk assessment by reducing the assumptions regarding consumption rates, residue occurrence, and processing effects and by integrating exposures from different sources (diet, household use, environmental). However, the relationship between exposure and concentration in human matrices is unknown for most pesticides. Therefore, we conducted a pilot study to gain more insight into the qualitative and quantitative relationship between dietary intake of pesticides (external exposure) and urinary excretion (reflecting internal exposure). In this cross-sectional observational study, 35 healthy consumers aged 18–65 years from the region of Wageningen, Netherlands, collected an exact duplicate portion of their diets during 24 h. On the same day, they also collected all their urine. The duplicate diets were analyzed using target screening by GC- and LC-HRMS; each duplicate diet contained at least five, up to 21, pesticide residues. The 24 h urine samples were analyzed using LC-HRMS in a suspect screening workflow. Metabolites were tentatively detected in all 24 h urine samples, ranging from six metabolites corresponding to four pesticides up to 40 metabolites originating from 16 pesticides in a single urine sample. In total, 65 metabolites originating from 28 pesticides were tentatively detected. After prioritization and additional confirmation experiments, 28 metabolites originating from 10 pesticides were identified with confidence level 1 or 2b. Next, quantitative analysis was performed for a selection of pesticides in duplicate diets and their metabolites in 24 h urine to assess quantitative relationships. In the quantitative comparisons between duplicate diet and 24 h urine, it was found that some metabolites were already present in the duplicate diet, which may give an overestimation of exposure to the parent pesticide based on measurement of the metabolites in urine. Additionally, the quantitative comparisons suggest a background exposure through other exposure routes. We conclude that suspect screening of 24 h urine samples can disclose exposure to mixtures of pesticide on the same day in the general population. However, more research is needed to obtain quantitative relationships between dietary intake and exposure.Graphical

Dietary Exposure to Glutamates of 2- to 5-Year-Old Toddlers in China Using the Duplicate Diet Method.

A duplicate diet collection method was used to estimate dietary exposure to glutamates in children aged 2-5 years in selected provinces of China. Daily duplicate diet samples were collected from 86 healthy toddlers over three consecutive days. Glutamates were analyzed using ultra-high-pressure liquid chromatography-MS/MS (UHPLC-MS/MS). Results showed that the highest glutamates content was found in mixed meals, at 5.12 mg/kg, followed by powdered formula (3.89 mg/kg), and milk and dairy products (2.29 mg/kg). The total mean daily dietary exposure for subjects was 0.20 mg/kg BW, and P95 daily dietary exposure was 0.44 mg/kg BW, both below the acceptable daily intake (ADI) (120 mg/kg BW) recommended by the Joint (FAO/WHO) Expert Committee on Food Additives (JECFA) and the ADI (30 mg/kg BW) set by the European Food Safety Authority (EFSA). Hence it can be considered that glutamates exposure would cause low risk in this group.

Dietary exposure of radionuclides and heavy metals in adult residents in a high background natural radiation area using duplicate diet method

Intake of radionuclides and heavy metals through food consumption is one of the important pathways for long-term health considerations. In this paper, the dietary exposure to radionuclides (210Pb, 210Po, 226Ra, 228Ra, 40K, 137Cs and 129I) and heavy metals (As, Hg, Pb, Cd and U) of adult residents in the high background natural radiation area (HBNRA) in Yangjiang, China, was comprehensively assessed using duplicate diet method. The estimated effective dose received by the inhabitants in HBNRA from ingestion of radionuclides was 0.33 mSv/y, and the associated lifetime cancer risk was 1.1 × 10–3. Both the dose and cancer risk to humans were at the acceptable range, and showed no difference between the HBNRA and the control area. With respect to heavy metals, the estimated daily intake of heavy metals (DIM) values for As, Hg, Pb, Cd and U in HBNRA were 0.47, 0.03, 15.0, 0.26 and 0.04 μg/kg bw/d, respectively, and the corresponding target hazard quotient (THQ) were 1.58, 0.09, 3.7, 2.56, 0.18. The DIM and THQ of Cd and U in HBNRA were similar to the control area, but the DIM and THQ of Pb were much higher than the corresponding values of 0.39 and 0.03 in the control area. The hazard index (HI) value of heavy metals in HBNRA was almost twice that of the control area. This suggests that the inhabitants in the HBNRA may have a health risk associated with the heavy metals.

Comparison of Methods for Estimating Discretionary Salt Intake in Field Settings

ObjectivesAccurate and precise estimates of discretionary salt (DS) intake are critical for designing salt fortification interventions and counseling on salt intake reduction. This study compared four methods of estimating DS intake among non-pregnant women of reproductive age (NPWRA) in northern India to inform the design of a trial of multiply-fortified salt. MethodsParticipants were NPWRA (18–49 y) in Punjab, India. Weighed food records (WFR), same-day duplicate diet (DD) composites, and samples of household (HH) salt were collected simultaneously from 100 women and repeated on a subset of 40. Sodium (Na) and iodine contents of the DD composites were analyzed using Inductively Coupled Plasma (ICP)-Optical Emission Spectrometry and ICP-Mass Spectrometry. HH salt samples were also analyzed for iodine. Methods for estimating DS intake included: 1) WFR: DS consumed from recipes or added at time of consumption were weighed; 2) HH salt disappearance (HHSD): total DS used by HH on the observation day divided by number of HH members; 3) Sodium estimation (NaE): Na content of 40 replicate DD composites prepared without DS were subtracted from the Na content of the corresponding original DD and difference multiplied by the molar mass of NaCl; 4) Iodine method (IM): analyzed iodine content of milk and milk products and commercial snacks were subtracted from DD iodine content, and difference divided by the iodine content of the HH’s salt sample. The relations between methods were explored using Pearson correlation and Bland Altman analyses. ResultsMean ± SD intake of DS according to the WFR, HHSD, and NaE methods were 4.7 ± 1.8 g/d, 5.8 ± 3.3 g/d, and 4.1 ± 2.1 g/d, respectively. Results of IM are pending. Pearson correlation coefficients for DS intake estimates obtained from WFR vs. NaE and WFR vs. HHSD were 0.82 (p < 0.001) and 0.48 (p < 0.001), respectively. Mean ± SD bias (limits of agreement) were 0.68 ± 1.25 g/d (–1.77, 3.13) for WFR vs. NaE, and 1.8 ± 2.93 g/d (–4.56, 6.92) for HHSD vs. WFR methods. ConclusionsDiscretionary salt intake from WFR and NaE showed good agreement and are feasible to implement in field settings. Funding SourcesBill & Melinda Gates Foundation.

Quantifying Indirect Contribution from Precursors to Human Body Burden of Legacy PFASs Based on Paired Blood and One-Week Duplicate Diet.

The restriction on legacy perfluoroalkyl substances (PFASs) has led to increasing application and contamination of their precursors and novel alternatives. However, the indirect contribution from precursors has not been well characterized. In this study, 24 PFASs were measured in the paired human blood and urine from general volunteers (n = 20), as well as their corresponding exposure matrices (7 day duplicate diet, drinking water and dust). Perfluorooctanoic acid (PFOA) was predominant, followed by 6:2 chlorinated polyfluoroalkyl ether sulfonate (6:2 Cl-PFESA), contributing 21.6-47.0 and 6.6-20.0% of the total concentrations, respectively. Total oxidable precursor (TOP) assay and isomeric analysis coupled with a toxicokinetic model suggested that around 19% of perfluorooctane sulfonate (PFOS) in human was contributed by its precursors. The strong correlation between the estimated daily intake (EDI) and human blood concentration for 6:2 Cl-PFESA suggested that it was mainly contributed by direct exposure. The bioavailability of 6:2 Cl-PFESA in the food matrices was estimated as 18.6% by comparing the estimated and measured blood concentrations, implying that human exposure might be overestimated if the bioavailability of PFASs in food was not considered. Assuming that they had a similar bioavailability, it was estimated that ca. 20% of PFOS body burden was from indirect exposure to its precursors, which was supported by TOP assay.

Quantitative analysis of organophosphate pesticides and dialkylphosphates in duplicate diet samples to identify potential sources of measured urinary dialkylphosphates in Japanese women

Increased levels of dialkylphosphates (DAP) in maternal urine are associated with a variety of adverse developmental outcomes in children. Although urinary DAP levels are usually considered to be a marker of exposure to organophosphate (OP) pesticides, excretion of DAP may also increase by ingesting preformed DAP. To date, no study has quantitatively assessed the possible contribution of the dietary intake of preformed DAP and OP pesticides to urinary levels of DAP. Therefore, we aimed to estimate the levels of 6 DAPs and 84 OP pesticides in duplicate diet samples and urine samples collected from 73 women living in urban areas of Japan in 2018. DAP and OP pesticides were detected in 94% and 45% of diet samples, while DAP was detected in 100% of urinary samples, respectively. The average daily intake of preformed DAP was significantly higher than that of parent OP pesticides in our participants. Dimethylphosphate and diethylphosphate were predominant in the preformed DAP, and the estimated average daily intake of total amount of DAP was 78.3 nmol. Fruits and vegetables were the major dietary sources of DAP. Dietary intake of DAP was positively associated with urinary DAP levels, suggesting that a considerable amount of urinary DAP was derived from ingesting preformed DAP. Our results show that attributing urinary DAP levels exclusively to OP pesticide exposure would result in a substantial overestimation of the exposure level. Therefore, the urinary levels of DAP may not be suitable for evaluating OP pesticide exposure in the general urban population.

Assessment of Dietary Exposure to Ochratoxin A in Lebanese Students and Its Urinary Biomarker Analysis.

The present study investigated the dietary and urinary OTA occurrence among 44 Lebanese children. Relying on HPLC-FLD analysis, OTA was found in all the urine samples and in 46.5% and 25% of the 24 h duplicate diet and dinner samples, respectively. The means of OTA levels in positive samples were 0.32 ± 0.1 ng/g in 24 h diet, 0.32 ± 0.18 ng/g in dinner and 0.022 ± 0.012 ng/mL in urines. These values corresponded to margin of exposure (MOE) means of 7907 ± 5922 (neoplastic) and 2579 ± 1932 (non-neoplastic) calculated from positive 24 h diet, while 961 ± 599 (neoplastic) and 313 ± 195 (non-neoplastic) calculated from the urine. Since the MOE levels for the neoplastic effect were below the limit (10,000), a major health threat was detected and must be addressed as a health institutions’ priority. Besides, the wide difference between PDIs and MOEs calculated from food and urine suggests conducting further OTA’s toxicokinetics studies before using urine to measure OTA exposure.

Arsenic exposure analysis for children living in central China: From ingestion exposure to biomarkers

Emerging evidence indicates that chronic low-dose arsenic (As) exposure can pose adverse health effects to children. This study aimed to systematically study the exposure risk induced by As ingestion in children living in Hubei Province, central China. The feasibility of first morning spot urine instead of 24-h urine as an environmental exposure biomarker was also explored. A total of 120 children aged 2–17 years were recruited from an urban area for the collection of biomarker samples (first morning and 24-h urine samples), environmental exposure samples (duplicate diets, drinking water, and soil), and related child-specific exposure factors. The external exposure risk, internal exposure level, and source of exposure to As in children were analyzed. The results indicated that As concentration in duplicated diets, water, and soil were 29.2 μg kg−1, 1.3 μg L−1, and 9.3 mg kg−1, respectively; these were all below the corresponding maximum allowable levels in China (the threshold value of As in most food, drinking water and soil are 0.5 mg⸱kg−1, 0.01 mg L−1, and 20 mg⸱kg−1, respectively). Dietary intake was the predominant exposure route, accounting for 90% of the total daily dose. The combined oral non-carcinogenic and carcinogenic risks all exceeded the corresponding maximum acceptable risk level. Therefore, As bioavailability should be investigated and used in health risk assessment. Multiple linear regression analysis indicated that urinary As was positively associated with dietary As, with a one-unit increase in daily As intake from the diet associating with 4.82 and 5.21 μg g−1 increases in 24-h urine and first morning urine, respectively. Furthermore, significant correlations with 24-h urine and external exposure metrics suggested that creatine-adjusted As concentrations in first morning urine could be an appropriate substitute of 24-h urine as exposure biomarkers.

Dietary intake and urinary excretion of methylated arsenicals of Japanese adults consuming marine foods and rice

ABSTRACT Dietary intake and urinary excretion of monomethylarsonic acid (MMA), dimethylarsinic acid (DMA) and arsenobetaine (AsB) were measured in 150 adult Japanese subjects. Duplicate diet (DD) samples and first void of urine on the next morning of DD sampling day were collected and analysed for arsenic species with liquid chromatography-ICP mass spectrometry. Median (min-max) intakes of MMA, DMA and AsB were <2.3 (<2.3-<2.3), 4.57 (<2.3–24.3), and 13.6 (<2.4–231) μg As/day, respectively, and median urinary concentrations were 1.90 (<0.37–26.), 21.9 (<0.27–141) and 19.6 (<0.37–1063) ng As/mg creatinine, respectively. Interrelationships between intake levels and urinary concentrations were mostly significant with positive coefficients indicating mutual association due to co-exposure, metabolism and/or dietary habit. Urinary concentrations and intake levels of AsB were also positively correlated confirming the applicability of urine analysis as biomarker of exposure. The present descriptive results provide with essential piece of information in assessing health risk of methylated arsenicals for population who consume marine products and rice.

Vitamin B12 may play a preventive role in esophageal precancerous lesions: a case-control study based on markers in blood and 3-day duplicate diet samples.

It is hypothesized that vitamin B12 may prevent tumor initiation during the early stage of carcinogenesis such as esophageal precancerous lesions (EPL), whereas an excessive level may promote tumor progression during the later stages of carcinogenesis. This study aimed to determine the role of vitamin B12 in EPL by detecting vitamin B12-related markers in both blood and diet. This case-control study based on 3-day duplicated diet samples was conducted in a high-risk area of Huai'an, China. A 100 EPL cases and 100 healthy controls matched by gender, age (± 2years) and villages were included. Dietary intake of vitamin B12 and cobalt, plasma cobalt level, the serum levels of vitamin B12 and transcobalamin II (TC II) were quantitatively analyzed. Dietary vitamin B12 intake (p for trend = 0.384) and plasma cobalt level (p for trend = 0.253) were not associated with EPL risk, but high dietary cobalt intake (p for trend = 0.034), increased serum levels of vitamin B12 (p for trend = 0.036) and TC II (p for trend < 0.001) were significantly associated with the reduced EPL risk. However, the significant negative association between dietary cobalt intake, plasma cobalt level or serum vitamin B12 level and EPL was only found in female or male subjects. Excellent transport capability of bio-active vitamin B12 in vivo and adequate levels of vitamin B12 and cobalt may play preventive roles in EPL. Additionally, the association between vitamin B12, cobalt and the risk of EPL may vary in different genders.

Estimation of dietary intake and sources of organohalogenated contaminants among infants: 24-h duplicate diet survey in Fukuoka, Japan

The widespread occurrence of persistent organic pollutants (POPs) in the environment is a matter of concern. In this study, selected organohalogenated contaminants, including dichlorodiphenyltrichloroethane and its metabolites (DDTs) polychlorinated biphenyls (PCBs), chlordanes (CHLs), hexachlorobenzene (HCB), polybrominated diphenyl ethers (PBDEs), hexabromocyclododecanes (HBCDs), tetrabromobisphenol A (TBBPA), 2,4,6-tribromophenol (TBP), were measured in complete meal sets (24-h duplicate-diet) of Japanese infants to investigate the levels, profiles, and possible sources of contamination. In total, 46 whole-day meals of infants (7–24-months old) were collected during 2017 from Fukuoka, Japan. To the best of our knowledge, this is the first report based on the duplicate-diet method for infants. The median intakes among the POP groups were highest for ΣDDTs (18 ng/day, maximum 251 ng/day), followed by ΣPCBs (17 ng/day, maximum 198 ng/day), ΣCHLs (14 ng/day, maximum 105 ng/day), HCB (11 ng/day, maximum 64 ng/day), TBP (3.5 ng/day, maximum 109 ng/day), ΣHBCDs (1.9 ng/day, maximum 70 ng/day), TBBPA (0.72 ng/day, maximum 34 ng/day), and ΣPBDEs (0.11 ng/day, maximum 4.5 ng/day). Among the PCBs, PCB-138 and PCB-153 were the most abundant congeners (27% and 23%, respectively). p,p’-DDE, the major DDT metabolite, accounted for 96% of total DDTs. Among PBDEs, BDE47 was the only detected congener (present in 4% of the samples). The dietary intake of the targeted compounds was lower than the intake via breast milk, suggesting that the exposure from baby food was limited. In the principal component analysis, chlorinated and brominated compounds were separated on principal component 1, while TBP and α-HBCD were separated on principal component 2, likely suggesting a differing emission time trend or source. PCB-153, PCB-138, trans-chlordane, cis-chlordane, and trans-nonachlor were correlated with seafood consumption (Spearman's ρ = 0.45 to 0.57, p < 0.05), while TBP was correlated with seaweed consumption (Spearman's ρ = 0.46, p < 0.05). Also, four species of commercial edible seaweed in Japan were analyzed to confirm the findings of the duplicate-diet study. The relatively high concentration of TBP (5.5 ± 6.6 ng/g wet weight) was observed in the seaweed samples, indicating that seaweed is a potential exposure source of TBP.

Comprehensive dietary and internal exposure assessment of deoxynivalenol contamination in a high-risk area in China using duplicate diet studies and urinary biomarkers

This study assessed the exposure of 199 Chinese population samples from the Anhui Province, China, to deoxynivalenol (DON) based on their dietary intake and urinary biomarkers. This is the first study to compare between both procedures of DON exposure detection (diet study and human bio-monitoring approach) within the same population. Food and urine samples were collected from 199 healthy participants. The highest concentrations of DON and its derivatives were detected in wheat-based products. Both DON and its metabolite, DOM-1, were found in the urine samples. Total (free + conjugated) DON was detected in 99% of the samples, with a mean concentration at 109.21 ng mL−1. The mean estimated dietary intake (EDI) level calculated from food consumption and contamination data was obtained as 2.54 ± 1.63 μg kg−1 bw d−1, while the mean probable daily intake (PDI) based on urinary biomarkers was estimated to be 3.96 ± 4.20 μg kg−1 bw d−1. A significant correlation was evidenced between EDI and PDI, both exceeding the provisional maximum tolerable daily intake. Elevated contaminant levels were observed in the adolescent group compared to children, adults, and elders, thereby indicating a potential health risk in them. The excretion rate of adolescents was approximately 2.5 times that of adult participants, possibly indicative of higher metabolic activity, which needs further in-depth investigation.

Dietary exposure to fatty acid esters of monochloropropanediols and glycidol of 2- to 3-year-old children attending nursery schools from two areas in China using the duplicate-diet collection method

ABSTRACT The dietary exposures to fatty esters of 3- and 2-monochloropropanediol (MCPD) and glycidol were estimated for children aged 2- to 3-year-old from two areas of China using duplicate diet collection method. The 24-h daily duplicate diet samples over three consecutive days were collected from 40 healthy children aged between 26 and 36 months. The analysis of these contaminates in food samples was measured by an indirect method that entails MCPD/glycidol cleavage from their esterified forms for GC-MS analysis. Over 71% of the mixed diet and dairy products samples were found to be contaminated with 3-MCPD and glycidyl esters. The estimated daily exposure to bound 3-MCPD (mean: 0.48–0.49 μg kg−1 bw day−1; P95: 1.00–1.11 μg kg−1 bw day−1) were well below the health guidance values and were considered of low safety concern. The daily exposure to bound 2-MCPD was estimated to be 0.031–0.038 μg kg−1 bw day−1 on average and 0.12–0.14 μg kg−1 bw day−1 for the P95 exposure. However, it was not possible to assess its risk due to the lack of health guidance value of 2-MCPD. The margin of exposure (MOE) estimates for the mean exposure to bound glycidol (0.10–0.12 μg kg−1 bw day−1) were far above 10000 and were considered of low safety concern. However, the margin of exposure estimates for the P95 bound glycidol exposure (0.41–0.45 μg kg−1 bw day−1) were below 10000 and indicated a health concern. Our data indicated that the mixed diet accounted for nearly 76% to 91% of bound MCPD and glycidol exposure. In addition, the follow-on formula was also an important source for the children aged 2–3 years.

Source Identification Analysis of Lead in the Blood of Japanese Children by Stable Isotope Analysis.

Considering the negative effect of lead (Pb) on children’s neurodevelopment, Pb exposure should be minimized to the lowest extent possible, though the blood Pb (BPb) concentrations in Japanese children are among the lowest in the world. To identify the sources of Pb in blood, isotope ratios (IRs: 207Pb/206Pb and 208Pb/206Pb) of Pb (PbIR) in whole blood from eight Japanese children were measured by multi-collector ICP mass spectrometry. Further, samples of house dust, soil, duplicate diet, and tobacco, collected from home environments, were also measured and were compared with PbIR of blood case by case. The relative contribution of Pb in the home environment to BPb were estimated by linear programming (finding an optimal solution which satisfy the combination of IRs and intakes from various sources) when appropriate. Source apportionment for three children could be estimated, and contributions of diet, soil, and house dust were 19–34%, 0–55%, and 20–76%, respectively. PbIR for the remaining five children also suggested that non-dietary sources also contributed to Pb exposure, though quantitative contributions could not be estimated. Non-dietary sources such as soil, house dust, and passive tobacco smoke are also important contributors to Pb exposure for Japanese children based on PbIR results.