Dual-mode Detection Research Articles

Nanowire-like phosphorus modulated carbon-based iron nanozyme with oxidase-like activity for sensitive detection of choline and dye degradation

Choline is mainly supplemented through food intake, lack of choline would result in diseases like liver cirrhosis, hardening of the arteries, or neurodegenerative disorders. The accurate detection of choline is important for human health. Herein, a novel nanowire-like phosphorus/nitrogen co-doped carbon-based iron nanozyme (Fe-NPC) with outstanding oxidase-like activity was synthesized, and the influence of phosphorus doping on oxidase-like activity was explored. Proper phosphorus doping was found to enhance the oxidase-like activity of Fe-NPC by increasing surface defects, promoting iron loading, and modulating chemical environment of central site. The oxidase-like activity of Fe-NPC was successfully applied to colorimetric-fluorescent dual mode detection of choline, and good linear relationship in the ranges of 3–200 μM were achieved with LODs of 1.95 and 1.50 μM, respectively. The fluorescent detection was constructed based on the fluorescence quenching of silicon quantum dots (Si QDs) by quinone-imine. The quenching mechanism was attributed to FRET due to the large spectra overlap, reduced fluorescence lifetime and proper donor−acceptor distance. The successful application to the detection of choline in milk proved the practicability of the proposed sensing method. The oxidase-like properties of Fe-NPC was further used in the degradation of cationic dye methyl blue, high degradation efficiency of 74 % was achieved within 5 min under optimal conditions, proving the enormous potential of Fe-NPC for application in environment protection.

Dual-Modality Accurate Visualization of Drug Synergy Based on Mass Spectrometry and Fluorescence Imaging.

There is a potential synergistic effect between nonsteroidal anti-inflammatory drugs and hydrogen sulfide (H2S), but direct evidence for the study is lacking. With a single fluorescence detection method, it is difficult to accurately confirm the effectiveness of the synergistic effect. In this study, the fluorescent probe and the nonsteroidal anti-inflammatory drug naproxen were combined via different self-immolative spacer groups to obtain a diagnostic and therapeutic integrated fluorescent probe Nap-NP-NSB, which can release H2S. The quantitative release of H2S by Nap-NP-NSB was evaluated in vitro and in cells, and the synergistic effect of H2S and naproxen was confirmed by monitoring the treatment process of cellular inflammation and oxidative damage of gastric mucosa cells. Finally, in vivo fluorescence imaging and mass spectrometry imaging of the liver and stomach tissues and their sections were performed in the mouse model of acute hepatitis. The dual-modal detection method not only confirmed that Nap-NP-NSB had better anti-inflammatory activity and less gastric mucosal damage, but also enabled a more accurate visualization of the drug synergistic effect of naproxen and H2S. This work provides a dual visualization imaging method combining fluorescence and mass spectrometry imaging and develops a new idea for studying drug synergies based on self-immolative structures.

Nitrogen and Sulfur Co-Doped Graphene-Quantum-Dot-Based Fluorescent Sensor for Rapid Visual Detection of Water Content in Organic Solvents.

Accurate water content detection is crucial for optimizing chemical reactions, ensuring product quality in pharmaceutical manufacturing, and maintaining food safety. In this study, nitrogen and sulfur co-doped graphene quantum dots (R-GQDs) were synthesized via a one-step hydrothermal method using o-phenylenediamine as the carbon source. The synthesis conditions, including reaction time, temperature, o-phenylenediamine concentration, and H2SO4/water ratio, were optimized using the Box-Behnken response surface methodology. The R-GQDs exhibited excellent fluorescence stability and distinct solvent-dependent characteristics, alongside a broad linear detection range and high sensitivity, making them highly suitable for dual-mode water content detection (colorimetric and fluorescent). To enhance the accuracy of visual detection, R-GQDs were incorporated into portable test strips with smartphone-assisted analysis, compensating for the human eye's limitations in distinguishing subtle color changes. The sensor's practical utility was validated through spiked recovery experiments in food samples, and the R-GQDs demonstrated good biocompatibility for in vivo imaging in shrimp. These findings highlight a novel strategy for developing portable, real-time water content sensors with potential applications in both portable detection systems and biological imaging.

A novel four-modal nano-sensor based on two-dimensional Mxenes and fully connected artificial neural networks for the highly sensitive and rapid detection of ochratoxin A

Timely and accurate on-site detection of ochratoxin A (OTA) is extremely important for global public health. In this study, a fluorescence/colorimetric biosensor based on Ti3C2 nano-materials (Ti3C2-NMS) and a machine-learning (ML) based fluorescence/colorimetric intelligent learning system for detection of OTA concentration (COTA) were developed. The sensor was fabricated by functionalizing Ti3C2-NMS prepared by physical-exfoliation assisted metal-ion-induction using ssDNA. The Ti3C2-NMS exhibited good fluorescence quenching characteristics (FQC) and peroxidase-like activity (PLA). More surprisingly, the functionalization of Ti3C2-NMS by ssDNA further enhanced the FQC and PLA of the material, which could be used for dual-mode detection of OTA. When different COTA existed, ssDNA competitively bound to OTA, resulting in regular changes in fluorescence and colorimetric signals of the sensor, which realized the accurate and sensitive biosensing detection of OTA in two modalities. Based on a series of fluorescent/colorimetric RGB datasets collected by a self-developed application, a dual-channel ML model had been developed. This model can be integrated into mobile phones, clouds, and PCs to achieve intelligent sensing detection of OTA with the assistance of fully connected artificial neural networks. The method constructed had high specificity, low cost, and fast responsiveness, with a LOD as low as 1.58 pg mL−1, indicating excellent potential for application and promotion.

Bi-MOF-based point-of-care testing paper for dual-mode detection of H2S

Bi-MOF-based point-of-care testing paper for dual-mode detection of H2S

Simple strategy for dual-responsive ratio electrochemical-colorimetric detection of nitrite in food and environment.

A dual-responsive ratio electrochemical-colorimetric method for nitrite (NO2-) is established based on the combination of nanoenzyme (Mn3O4) catalysis with diazotization reactions. The Mn3O4 can oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue TMBox. The NO2- induces the diazotization reaction of TMBox, leading to a decrease of the signal at 652nm and the generation of a new signal from diazotized TMBox at 445nm. Furthermore, the presence of NO2- reduces the electrochemical oxidation signal of TMB and simultaneously provides its electrochemical signal. Compared withtraditional single-mode detection, dual-mode detection offers higher sensitivity, lower detection limits, and better interference resistance. The inherent advantages of this method make it feasible to detect NO2- in real samples, offering broad prospects for applications in food safety and environmental monitoring.

Photoinduced Zn-Air Battery-Assisted Self-Powered Sensor Utilizing Cobalt and Sulfur Co-Doped Carbon Nitride for Portable Detection Device.

Most self-powered electrochemical sensors (SPESs) are limited by low open circuit voltage and power density, leading to a narrow detection range and low sensitivity. Herein, a photoinduced Zn-air battery-assisted SPES (ZAB-SPES) is proposed based on cobalt and sulfur co-doped carbon nitride with the cyano group (Co, S-CN). The cyano functionalization remarkably enhances visible light utilization, and the cyano moiety acts as an electron-withdrawing group to promote electron enrichment. Co and S co-doping can create a p-n homojunction within carbon nitride, enabling the efficient migration and separation of carriers, thereby significantly improving the performance of the oxygen reduction reaction. The synergistic effects endow Co, S-CN photocathode with an open circuit voltage of 1.85V and the maximum power density of 43.5µW cm-2 in the photoinduced ZAB. Employing heavy metal copper ions as the target model, the photoinduced ZAB-SPES exhibited dual-mode and sensitive detection. Furthermore, a portable detection device based on the photoinduced ZAB-SPES is designed and exhibits high linearity in the range of 5 ~ 600nM with a detection limit of 1.7nM. This work offers a portable detection method based on the photoinduced ZAB-SPES in the aquatic environment.

A dual-mode strategy for early detection of sugarcane pokkah boeng disease pathogen: A portable sensing device based on Cross-N DNA framework and MoS2@GDY

Sucrose, a common sugar primarily derived from sugarcane, is a crucial national strategic resource. However, its yield is significantly affected by various serious diseases, with pokkah boeng disease being one of the most damaging. Therefore, developing a sensitive method for the accurate detection of the pokkah boeng disease pathogen is crucial for ensuring the safety of sugar. This work presents a portable dual-modal detection device, assisted by a smartphone, which is based on MoS2@GDY, Mn3O4@Au nanomenzyme, cross-N DNA framework and Exo III exonuclease-assisted CHA signal amplification technology. The cross-N DNA framework provides many binding sites and is not restricted by AuNPs scattering positions, enhancing the signal output strength of the sensor. Additionally, the detection system incorporates a high-power-density capacitor to further amplify the electrochemical detection signal, increasing sensitivity by 9.1 times. Moreover, the use of electrochemical and colorimetric dual-mode detection effectively avoids mutual interference, reducing the likelihood of false positives from a single signal. Under optimized conditions, the proposed method has a linear range of 0.0001–10,000 pM, and with a detection limit of 6.1 aM (S/N=3). This high-sensitivity, high-reliability portable sensing method shows significant potential for the early detection and real-time on-site monitoring of the pokkah boeng disease pathogen.

An innovative homogeneous electrochemistry coupled with colorimetry dual-model sensing strategy for perfluorooctane sulfonate based on Cu@CuO aerogel nanozyme.

Bypreparing Cu@CuO aerogel as a nanozyme which exhibits prominent peroxidase-like (POD) activity, an innovative homogeneous electrochemistry (HEC) coupled with the colorimetry dual-model sensing strategy is proposed to detect perfluorooctane sulfonate(PFOS) for the first time. Cu@CuO aerogel accelerates the oxidation process of colorless o-phenylenediamine to form yellow 2,3-diaminophenazinc (DAP), and meanwhile, DAP as an electroactive substance creates a reduction peak current upon the electrochemical measurements. Interestingly, in the presence of PFOS, the POD activity of Cu@CuO aerogel isinhibited since the specific coordination between PFOS and Cu(II) can cover the active sites, resulting in the color of the sensing system becoming light and the peak current of DAP decreasing. This innovative dual-mode detection method showed excellent electrochemical detection of PFOS in the concentration range 10.0 ~ 1125.0nM with a limit of detection (LOD) as low as 3.3nM and a LOD of 20.8nM in the colorimetric detection in the range 62.3 ~ 875nM. Furthermore, the sensor was successfully used for the analysis of real samples with an RSD value ≤ 6.5%. The successful application of this two-mode sensing method for the determination of PFOS holds promise for the detection of other contaminants in the future.

Wheat Germ Agglutinin-Modified "Three-in-One" Multifunctional Probe Driven Broad-Spectrum and Flexible Immunochromatographic Diagnosis of viruses With High Sensitivity.

The conventional lateral flow assay (LFA) fails to the demands for the accurate screening of viruses as a result of its low sensitivity of colorimetric signal output and poor universality limited by antibody pairs. Here, a magnetically assisted dual-signal output LFA platform is developed for the ultrasensitive, universal, and flexible detection of viruses. A "three-in-one" multifunctional probe (MAuDQD) is prepared using a 180nm Fe3O4 core to load numerous Au nanoparticles (NPs) and two layers of QDs, which can substantially improve the sensitivity of LFA through coupling with the effects of magnetic enrichment and colorimetric/fluorescent enhancement. Wheat germ agglutinin (WGA)-modified MAuDQD attained the broad-spectrum capture viral membrane proteins and the colorimetric/fluorescent dual-mode detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and monkeypox virus (MPXV) on the LFA strip. In the colorimetric mode, the target viruses detected directly, with the visual sensitivity reaching 0.1-0.5ngmL-1 and the fluorescent mode supported quantitative analysis of SARS-CoV-2/MPXV with limits of detection decreasing to pgmL-1 level. Practicability of the MAuDQD@WGA-LFA is verified through the detection of 33 real clinical samples, showing the proposed assay has a great potential to become a sensitive, accurate, and universal tool for on-site monitoring of viral infections.

Enzyme cascade signal amplification platform with immobilized xanthine oxidase on nanozymes exhibiting enhanced POD-like activity for ultrasensitive dual-mode detection of xanthine

Xanthine (XA) metabolic disorders cause many diseases, hence early and precise XA detection is essential. Herein, a dual-mode enzyme cascade nanosensing platform based on Au/FMZIF-rGO/PCN nanomaterial with dual-emissive and high peroxidase-like (POD-like) activity was devised. The rapid electron transfer between Fe, Mn and P elements and the formation of ZIF-rGO/PCN hybrid enhanced POD-like activity. Aggregation-induced emission (AIE) effect improved fluorescence intensity of AuNCs. Furthermore, utilizing the nanozyme to immobilize xanthine oxidase (XOD) also reduced intermediate product diffusion, improving cascade efficiency and platform detection·H2O2 was catalytically decomposed to generate •OH, lowering fluorescence intensity at 627 nm and oxidizing TMB to produce blue oxTMB. So, dual-mode nanosensing platform was constructed with linear ranges for ratio fluorescence and colorimetric detection of XA spanning 0.5–200 μM and 0.5–300 μM, respectively, and corresponding LODs of 0.11 and 0.24 μM. The nanoplatform suggested promising potential for practical clinical detection of XA and other biomarkers.

Ultrabright silicon nanoparticles combined with o-phenylenediamine for ratiometric fluorescence and smartphone imaging dual-mode detection of nitrite

Nitrite (NO2−) is widely present in the natural environment and human daily life. Excessive NO2− can cause harm to the environment and human health. Herein, silicon nanoparticles (SiNPs) with a fluorescence quantum yield of up to 70 % were synthesised using a one-pot hydrothermal method and combined with the common and inexpensive o-phenylenediamine (OPD) to achieve the detection of NO2−. Upon the addition of NO2−, the blue fluorescence of the SiNPs was quenched due to static quenching and Förster resonance energy transfer (FRET), while the yellow fluorescence of benzotriazole, the reaction product of OPD and NO2−, was enhanced, resulting in the fluorescence color change from blue to yellow. Based on these phenomena, a ratiometric fluorescence sensor integrated with smartphone imaging technology was developed. This sensor is notable for its portability, cost-effectiveness, and satisfactory detection limits (0.016 μM for ratiometric fluorescence and 1.64 μM for smartphone imaging). Importantly, it demonstrates high reliability and practicability in detecting NO2− in real water and food samples. This broadens the application of SiNPs in the sensing field and introduces new possibilities for NO2− detection in complex sample matrices.

A novel double-door-opening sensor for visual determination of cardiac troponin I based on DNA hydrogel and bimetallic nanozyme

Cardiac troponin I (cTnI), as a cardiac biomarker, holds significant importance in the diagnosis of acute myocardial infarction. However, the current detection methods mostly require specialized personnel and large analytical instruments, making it difficult to achieve convenient and on-site testing. This situation leads to delayed disease diagnosis and treatment, that increases patient suffering, and reduces the cure rate. This strategy presents the development of a portable visual DNA hydrogel colorimetric sensing platform based on a smartphone. Utilizing dual-mode detection with ultraviolet signals and solution colorimetry, the platform achieves ultra-sensitive and real-time detection of cTnI. Furthermore, optimization of detection conditions, such as the amount of polyacrylamide, reaction time, aptamer concentration, encapsulation of the nanozyme, incubation time, and reaction temperature, were performed based on this platform. The proposed ultraviolet and visual detection platforms exhibited good linear relationships with the signal within the ranges of 0.003 ng mL−1 to 10.00 ng mL−1 and 0.01 ng mL−1 to 7.00 ng mL−1, with detection limits of 2.57 pg mL−1 and 0.013 pg mL−1, respectively. Additionally, utilizing 3D printing technology, a portable detection device was designed and employed for the detection of cTnI concentrations in human serum samples. Whatever in the initial and spiked samples, the results showed high sensitivities. The sensitivity and convenience of the sensor in detecting cTnI make it promising for home testing of patients, with broad market prospects.

Reactive Fluorescent and Colorimetric Probe for Highly Selective and Sensitive Detection of Hg2+ in Real Water Samples.

Construction of efficient chemosensors for highly specific and sensitive detection of mercury ions remains a great challenge. In this work a highly selective and sensitive probe CY was designed and synthesized by using coumarin fluorophore as the matrix and thioacetal moiety as the reactive recognition site for Hg2+. By virtue of the thiophilicity of Hg2+, probe CL could be hydrolyzed to deprotect and the thioacetal was transformed to the acyl group after the addition of Hg2+, the blue-green fluorescence was quenched and meanwhile the solution changed from light green to yellow. The detection limit of probe CY for Hg2+ was as low as 6.8 nM, and it could completely react with Hg2+ within 3min. Moreover, probe CY exhibited good resistance against interference from competitive metal ions and biothiols, high stability in pH 1-11 and applicability for fluorogenic and chromogenic dual-modal detection of Hg2+ in real water samples over a broad range of pH 5-10.

Cascade enzyme-mimicking with spatially separated gold-ceria for dual-mode detection of superoxide anions

Metal-semiconductor nanozyme of dumbbell Au-CeO2 with spatially separated heterostructure has cascade superoxide dismutase (SOD)-like and peroxidase (POD)-like activities for superoxide anions detection. It was synthesized by selective growth of CeO2 at the ends of Au nanorod (Au NR). Taking advantage of the excellent local surface plasmon resonance (LSPR) effect of Au NR, the spatially separated Au-CeO2 has a higher photothermal effect than the continuously growing core-shell structure of Au@CeO2. Meanwhile, the hot electrons of Au NR could transfer to CeO2 under 808 nm laser irradiation, changing the ratio of Ce3+/Ce4+ redox couples over CeO2 and facilitating H2O2 decomposition thus enhancing POD-like activity. Based on the SOD-like activity of Au-CeO2, superoxide anion (O2·−) can be transformed into hydrogen peroxide (H2O2). Dual-mode including absorbance and temperature sensing detection of O2·−, with the detection range from nM to μM i.e., 0.1–150 μM and LOD of 0.033 μM (S/N = 3) was achieved through the cascade catalysis and photothermal effect. The as-proposed method was applicable to both cancer and normal cell samples with satisfactory accuracy and recovery.

UAV Hunter: A Net-Capturing UAV System with Improved Detection and Tracking Methods for Anti-UAV Defense

The abuse of UAVs poses a potential risk to social security, necessitating the investigation of anti-UAV methods to safeguard critical areas. However, the existing UAV countermeasures face challenges such as high environmental impact, restricted spatial deployment, and low cost-effectiveness. To address these limitations, we developed a novel anti-UAV system known as UAV Hunter, which adopts an airborne tether-net capture device with visual aids to counter unauthorized UAVs. This system employs an “Anti-UAV with UAV” scheme, comprising a ground control station and a net-capturing UAV. The operator utilizes the ground control station to determine the mission area and flight path and then controls the flight of the net-capturing UAV. During flight, the net-capturing UAV leverages its dual-mode sensor to continuously monitor the target area. Simultaneously, the onboard computer executes a UAV detection and tracking algorithm to search for unauthorized UAVs in real time. The results are relayed to the operator in real time, facilitating precise adjustments for the net-capturing UAV to launch the rope net accurately. The system successfully realizes the functions of dual-mode real-time detection and tracking, precise net capture, and efficient integrated control. Compared with existing methods, the developed system exhibits accurate recognition, rapid action, diverse application scenarios, and an enhanced human–machine interaction experience. Test results in the open environment further validate the feasibility and functional integrity of the system, demonstrating its capability to effectively capture low-altitude unauthorized UAVs.

CRISPR/Cas12a dual-mode biosensor for Staphylococcus aureus detection via enzyme-free isothermal amplification

Accurate and reliable detection of Staphylococcus aureus (S. aureus) is essential for preventing infections, particularly in healthcare and food safety contexts. This work presents a novel dual-mode biosensor that integrates the CRISPR/Cas12a system with an enzyme-free isothermal amplification method for detecting S. aureus. Hybridization chain reaction (HCR) and catalytic hairpin assembly (CHA) amplify the aptamer-triggered response, significantly enhancing sensitivity. CRISPR/Cas12a′s nuclease activity is utilized in two modes: cis cleavage generates a fluorescence signal, while trans cleavage produces an electrochemical signal, enabling dual-mode detection. The biosensor demonstrates outstanding performance, with a limit of detection (LOD) as low as 5.7 CFU mL−1 in electrochemical mode and 133.7 CFU mL−1 in fluorescence mode, showcasing excellent accuracy, stability, and sensitivity. It has been successfully applied to detecting actual samples, confirming its practical applicability. This innovative approach offers a powerful tool for the swift and precise identification of S. aureus and paves the way for developing next-generation dual-mode biosensors for various analytes. Future research will aim to simplify the detection process further, making it more accessible for use in resource-limited settings.

Dual-modal detection of antimicrobial susceptibility in pathogenic bacteria based on the high-throughput microfluidic platform

For pathogenic bacterial infectious diseases such as sepsis, accurate and rapid antimicrobial susceptibility testing (AST) plays an important role in improving antimicrobial screening and clinical outcomes. Traditional culture-based AST assays have the limitations of time-consuming, labor-intensive, expensive, and consuming reagents. In this work, a simple, fast, high-throughput microfluidic platform integrated with isolation, identification, and detection functions was designed for antibiotic susceptibility analysis in pathogenic bacteria. For the above purpose, MnO2@ZIF-90 nanoprobes with dual functions of nanozyme and fluorescence were prepared for visual eye or RGB analysis through a smartphone APP. More importantly, the redox activity of bacterial membrane in active pathogenic bacteria could decompose the MnO2 of MnO2@ZIF-90 nanoprobes and reduce the enzyme-like activity, which weakens the colorimetric signal of Timebox. Meanwhile, the ATP released from active pathogenic bacteria further triggered the breakdown of the inner ZIF-90 layer, generating a fluorescence signal. Therefore, a dual detection combining colorimetric and fluorescence was used to perform more accurate and reliable antibiotic susceptibility analysis of pathogenic bacteria. With the proposed microfluidic platform, a low detection of 10 CFU mL−1 bacteria and fast AST results were achieved within 5 min. The clinical applicability of the platform was further demonstrated through the analysis of clinical samples. This work provides a low-cost, easy-to-operation, fast-response, and high-throughput platform for the AST assays in pathogenic bacterial infectious diseases such as sepsis.

Colorimetric and fluorescence dual-mode detection of oxytetracycline based on hemin/G-quadruplex DNAzyme and ZIF-8

With the gradual increase of oxytetracycline (OTC) residue in the environment, it is particularly important to establish a sensitive and efficient method to detect OTC. In this work, a novel colorimetric and fluorescence dual-mode detection method based on hemin/G-quadruplex (hemin/G4) DNAzyme and zeolitic imidazolate framework-8 (ZIF-8) is developed. OTC can compete with G4 to bind hemin to reduce the amount of hemin/G4. Thus, the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to blue color by hemin/G4 is inhibited. In addition, the OTC or OTC-hemin can be aggregated with the help of ZIF-8 to produce strong green fluorescence due to the aggregation-induced emission (AIE) effect. In colorimetric and fluorescence mode, the linear ranges of the new method are 0.375–18 μM and 0–52.5 μM, with a limit of detection (LOD) of 0.116 μM and 0.040 μM, respectively. Besides, this new method has a good selectivity and recovery rate in the detection of real samples. Therefore, the method can be used for the efficient and sensitive detection of OTC.

A highly sensitive dual-mode detection platform based on the novel copper/molybdenum bimetallic nanoclusters and Co–Fe layered doubled hydroxide nanozyme for butyrylcholinesterase activity sensing

Herein, a novel copper/molybdenum bimetallic nanoclusters (Cu/Mo NCs) with intense blue emission were synthesized by using polyvinylpyrrolidone (PVP) as template and ascorbic acid as reducing agent. Owing to the synergistic effect between Cu and Mo, the fluorescence intensity of Cu/Mo NCs was significantly improved about 6-time than monometallic copper nanoclusters. A novel and sensitive ratiometric fluorescence and colorimetric dual-mode sensing platform for monitoring butyrylcholinesterase (BChE) was strategically constructed by the integration of Cu/Mo NCs with excellent optical properties and Co–Fe layered doubled hydroxide (CoFe-LDH) with superior peroxidase-like activity for the first time. In the presence of H2O2, nonfluorescent and colorless o-phenylenediamine (OPD) was oxidized to fluorescent and yellow 2,3-diaminophenazine (DAP) with maximum fluorescence emission peak at 564 nm and ultraviolet absorption peak at 418 nm by CoFe-LDH with peroxidase-like activity. Simultaneously, the generation of DAP could effectively quench Cu/Mo NCs fluorescence at 444 nm through the inner-filter effect (IFE). The hydrolysis of S-butyrylthiocholine iodide (BTCh) can be catalyzed by butyrylcholinesterase (BChE) to generate thiocholine (TCh) that could hinder the oxidation of OPD, leading to the fluorescence and ultraviolet absorption of DAP decreased, meanwhile, the fluorescence of Cu/Mo NCs recovered. The ratiometric fluorescence signal F564/F444 and colorimetric system both performed a satisfactory response to the concentration of BChE in the range 0.5 to 90 U L−1 and 1 to 100 U L−1 with the LOD of 0.18 U L−1 and 0.36 U L−1, respectively. The dual-mode sensing for BChE exhibited outstanding application potential in biosensing.