The photocatalytic antibacterial activity of a nickel-doped titanium dioxide nanoparticle mesh (Ni–TiO 2 mesh) against E. coli was investigated in chlorinated water under ultrashort-term dual ultraviolet (UV) irradiation. Ni–TiO 2 nanoparticles (NPs) were prepared via Ni doping to TiO 2 NPs and uniformly distributed on the mesh via electrospraying and hot-pressing, of which the morphology and particle size distribution were maintained after immobilization. In the Ni–TiO 2 mesh, the elements, Ti, O, Ni, iron, chromium and silicon were included suggesting multi- and mixed metal oxides. Irradiation distance and lamp cleaning were analyzed as controllable factors to maintain the dual UV intensity. The Ni–TiO 2 mesh showed a significant antibacterial activity even at the smallest mesh size of 0.25 cm 2 under 5-sec irradiation after the UV intensity adjustment. The results suggest that the improved NP characteristics on a steel mesh substrate, including multi- and mixed metal oxides, can display photocatalytic antibacterial activity against E. coli along with minimization of NP exposure due to NP immobilization. This can be directly extended to other environmental, industrial, and clinical applications as a safe and sustainable photocatalytic antibiotic agent for photocatalytic disinfection. • Dual UV-irradiated Ni-TiO 2 mesh was developed as a next-generation photocatalytic antibiotic agent for disinfection. • Free Ni-TiO 2 NPs were immobilized on the steel mesh via electrospraying and hot-pressing for improved characteristics. • Under ultrashort-term dual UV irradiation, the Ni-TiO 2 mesh had a significant antibacterial activity against Escherichia coli in chlorinated water, compared to UV alone. • The Ni-TiO 2 mesh can be promisingly extended to combined disinfection for enhanced antibiotic performance under ultrashort-term dual UV irradiation.
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