Dual Line Research Articles

Evaluation research on the independent controllability of high-end equipment manufacturing industry based on dual incentive control line: an empirical analysis from Jiangsu Province

PurposeIn order to analyze the level of independent controllability and its evolution of high-end equipment manufacturing industry from Jiangsu Province, this article introduces the dual-excitation control line method to construct a comprehensive evaluation model for independent controllability.Design/methodology/approachThrough the collection of information of high-end equipment manufacturing industry's independent and controllable capabilities on different indicators, the three aspects of advancement, autonomy and controllability, an empirical evaluation of 10 enterprises in the high-end equipment cluster in Jiangsu Province was conducted in terms of advancement, autonomy and controllability.FindingsIt effectively reveals the area and evolution characteristics of the “reward” and “punishment” of different indicators of each representative enterprise and reflects the development status and different characteristics of each representative enterprise on the three indicators. The research results provide decision-making guidance for enterprises in the management and control of advanced manufacturing systems with independent and controllable capabilities.Originality/valueExisting research focuses on the evaluation of enterprises' independent controllability only on a single angle or index. This paper maps the dynamic evaluation problem of multiple time-point data to the evaluation problem of single time-point multi-index data and investigates the fluctuation of the performance of the same enterprise under different indexes, so as to comprehensively evaluate the independent controllable level of high-end equipment manufacturing industry and analyze the reasons. Further, this paper first establishes an evaluation index system of independent controllable level of high-end equipment manufacturing industry and quantitatively measures the advanced, independent, controllable and other aspects of typical enterprises in this industry by constructing a double incentive control line evaluation model.

Efficient single and broadband microwave rectifiers for RFEH/WPT enabled low power 5G sub-6 GHz devices

In recent years, the enormous innovation in the wireless system has escalated the demands of fifth-Generation (5G) enabled RF Energy Harvesting (RFEH) and Wireless Power Transfer (WPT) systems. With this motivation, the proposed work introduces two efficient rectifier configurations for potential implementation in both RFEH and WPT systems covering global Sub-6 GHz 5G bands. The Design-1 presents a single-band rectifier covering 3.3–3.7 GHz sub-6 GHz 5G band. A dual Transmission Line (TL) based Matching Network (MN) is used to improve the rectifier performance. At 0 dBm, Design-1 achieved maximum Power Conversion Efficiency (PCE) of 76 % at 3.5 GHz and 4kΩ load. The Design-2 presents a 3.3–5 GHz broadband rectifier circuit that covers the entire sub-6 GHz 5G bands. A MN based on multi-stepped TL is used to improve the rectifier performance for the broad frequency band and achieved a PCE greater than 35 % and 50 % at 0 and 9 dBm, respectively at a load value of 1kΩ. For validation, prototypes of both rectifiers are fabricated and a very good agreement between simulated and measured results is achieved. The proposed rectifier configurations hold immense potential for implementation across nations and offer a global solution to self-sustainable low-powered 5G battery-less devices.

Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids.

During development of the human cerebral cortex, multipotent neural progenitors generate excitatory neurons and glial cells. Investigations of the transcriptome and epigenome have revealed important gene regulatory networks underlying this crucial developmental event. However, the posttranscriptional control of gene expression and protein abundance during human corticogenesis remains poorly understood. We addressed this issue by using human telencephalic brain organoids grown using a dual reporter cell line to isolate neural progenitors and neurons and performed cell class and developmental stage-specific transcriptome and proteome analysis. Integrating the two datasets revealed modules of gene expression during human corticogenesis. Investigation of one such module uncovered mTOR-mediated regulation of translation of the 5'TOP element-enriched translation machinery in early progenitor cells. We show that in early progenitors partial inhibition of the translation of ribosomal genes prevents precocious translation of differentiation markers. Overall, our multiomics approach proposes novel posttranscriptional regulatory mechanisms crucial for the fidelity of cortical development.

Optical tunable multifunctional applications based on graphene metasurface in terahertz

Due to the superior properties of graphene and the application potential of surface plasmons, the research of graphene surface plasmons has become a hot research direction. Based on the surface plasmons of graphene, this paper has done some researches on the plasma induced transparency, absorption, and slow light effect. The main work and results of this paper are as follows: we have designed a graphene-based metamaterial structure that can realize a dual plasma induced transparency (PIT) effect. The specific structure is formed by the periodic arrangement of graphene bands (as bright mode) and band edge microchips (as bright mode). We use the finite-difference time-domain (FDTD) method to study the dual PIT effect from the aspect of numerical simulation, and then further study the phenomenon of this device from the theoretical fitting of the coupled mode theory (CMT). The CMT model explores the physical mechanism of dual PIT spectral line and obtains a good fitting result. By studying the formation mechanism of the dual PIT effect, we have found that the graphene band as a bright mode interacts with the band edge microchip as a dark mode, and then the dual PIT is formed by destructive interference of the bright and dark modes. In order to better external modulation, the structure only studies the modulation effect caused by the change of Fermi level affected by the external voltage of graphene. Moreover, we also have studied the slow light performance of this structure, and the slow light coefficient reached 0.236 picoseconds (ps). This proposed coupling system of dual PIT effect has important research significance in optical switches, optical loop, and slow light devices.

Light-activated SAW sensor structures with photoconductive polymer films for DMMP detection

We report the light-activated surface acoustic wave (SAW) investigations of DMMP (dimethyl methylphosphonate) sensing based on photoconductive polymer films such as: (rr)P3HT (regio-regular Poly-3-hexylthiophene) and SilPEG1.4. These SAW sensor structures were composed of ∼205 MHz quartz dual delay lines with a novel switchable digital generator set-up and a thin (∼50–100 nm) polymer films created by means of a simple spray coating method. The white light, low power laser LED (blue, green, red) and seven LED’s sources were applied for light activation of the SAW structures. The relative sensitivity increase (frequency shifts in light vs. the non activated state) at 2 ppm of DMMP is ∼275 for SilPEG1.4 with a blue LED (∼460 nm), to even ∼340 for (rr)P3HT with a yellow LED (∼585 nm) activation. This last structure has also acceptable stability and repeatability with the limit of detection ∼133 ppb, the response/recovery times ∼5–100 s / ∼240 s, and sensitivity coefficient ∼1.013 kHz/ppm in ppb ranges. The observed substantially enhanced light-activated SAW sensitivity should be interpreted in category of the acoustoelectric interactions, because the acoustoelectric parameter, estimated by resistive transducer results, has the greatest value for a yellow and blue LED light illumination.

HPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease.

The enteric nervous system (ENS) is derived from both the vagal and sacral component of the neural crest (NC). Here, we present the derivation of sacral ENS precursors from human PSCs via timed exposure to FGF, WNT, and GDF11, which enables posterior patterning and transition from posterior trunk to sacral NC identity, respectively. Using a SOX2::H2B-tdTomato/T::H2B-GFP dual reporter hPSC line, we demonstrate that both trunk and sacral NC emerge from a double-positive neuro-mesodermal progenitor (NMP). Vagal and sacral NC precursors yield distinct neuronal subtypes and migratory behaviors invitro and invivo. Remarkably, xenografting of both vagal and sacral NC lineages is required to rescue a mouse model of total aganglionosis, suggesting opportunities in the treatment of severe forms of Hirschsprung's disease.

UNEXPECTED CURVES IN ℙ2, LINE ARRANGEMENTS, AND MINIMAL DEGREE OF JACOBIAN RELATIONS

We reformulate a fundamental result due to Cook, Harbourne, Migliore and Nagel on the existence and irreducibility of unexpected plane curves of a set of points Z in ℙ2, using the minimal degree of a Jacobian syzygy of the defining equation for the dual line arrangement 𝒜Z. Several applications of this new approach are given. In particular, we show that the irreducible unexpected quintics may occur only when the set Z has the cardinality equal to 11 or 12, and describe five cases where this happens.

Codeployment of Passive Samplers and Mussels Reveals Major Source of Ongoing PCB Inputs to the Anacostia River in Washington, DC.

Remedial investigations of sites contaminated with legacy pollutants like polychlorinated biphenyls (PCBs) have traditionally focused on mapping sediment contamination to develop a site conceptual model and select remedy options. Ignoring dissolved concentrations that drive transport and bioaccumulation often leads to an incomplete assessment of ongoing inputs to the water column and overestimation of potential effectiveness of sediment remediation. Here, we demonstrate the utility of codeployment of passive equilibrium samplers and freshwater mussels as dual lines of evidence to identify ongoing sources of PCBs from eight main tributaries of the Anacostia River in Washington, DC, that has been historically polluted from industrial and other human activities. The freely dissolved PCB concentrations measured using passive samplers tracked well with the accumulation in mussels and allowed predictions of biouptake within a factor of 2 for total PCBs and a factor of 4 for most congeners. One tributary was identified as the primary source of PCBs to the water column and became a focus of additional ongoing investigations. Codeployment of passive samplers and mussels provides strong lines of evidence to refine site conceptual models and identify ongoing sources critical to control to achieve river water quality standards and reduce bioaccumulation in the aquatic food web.

Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters

TBX5 and NKX2-5 are two important transcription factors regulating cardiomyocytes commitment. In this study, we generated a dual fluorescent reporter cell line based on H9 human embryonic stem cells (hESCs) and called it H9-NKX2.5GFP/TBX5Td-T2A-18, which could be short for T2A-18. When T2A-18 was induced towards a cardiomyocyte fate, EGFP and Tdtomato fluorescences were observed, indicating the expression of NKX2.5 and TBX5, respectively. Meanwhile, T2A had normal karyotype and could maintain main characteristics of wildtype H9. Therefore, T2A-18 could be used as a tool to help us study the mechanism of cardiomyocytes specification from hESCs.

Circularly Polarized Conformal Antenna with Dual V-shaped Coupling Feed Lines

Circularly Polarized Conformal Antenna with Dual V-shaped Coupling Feed Lines

Solanum pseudo-capsicum effects on Bax and Bcl-2 gene expression and apoptosis in MCF-7 cell line

In the present study, the effect of Solanum pseudo-capsicum extract on MCF-7 cell line was investigated. Control cell line in this study was HFF skin fibroblast cell line. Cells have been exposed to the treatment for 24h, 48h and 72 hours respectively. Then, the viability of dual cell lines was assessed using MTT assay. Its results showed that 72-hour treatment with plant extract at IC50 concentration had the greatest effect on cancer cell death. Flow cytometry results showed that 48 hours of treatment with plant extract at IC50 concentration, the highest rate of induced apoptosis occurs. Then, Real time PCR test was performed to measure changes in the expression of Bax and Bcl-2 genes The reference gene in this study was ?-actin. The results of this study showed that in cancer cells treated with the plant extract, the expression of Bax pro-apoptotic gene increased and the expression of anti-apoptotic gene Bcl-2 decreased. Therefore, 72-hour treatment had the greatest effect on increasing Bax gene expression and decreasing Bcl-2 gene expression. Due to the lower side effects of plants compared to conventional chemical drugs used in the treatment of cancer, it is possible to take an effective step in the treatment of this disease by using new methods in identifying effective plant compounds and purifying them.

Real-time measurement for boresight vibration of dual line scan cameras

The predecessor of China Optics was China Optics and Applied Optics Digest, which was founded in 1985. At that time, it was the only retrieval journal in the field of optics in China. At the end of 2008, China Optics and Applied Optics Digest was renamed

Real-time measurement for boresight vibration of dual line array surveying and mapping cameras

Real-time measurement for boresight vibration of dual line array surveying and mapping cameras

Smartphone Wireless charging

Smartphones are gradually becoming the most important personal device. In the first quarter of 2013, global smartphone shipments reached 216 million, which is approximately twice as large as in 2011.[1] In the absence of breakthroughs in mobile phone batteries nowadays, mobile phones are unable to run for long periods. For devices to work continuously, the convenience of their charging method is important. Wireless charging is a better solution to get rid of the charging cable and the convenience it brings. One of the main objectives of this paper is to find ways to improve the efficiency of wireless charging by analyzing different designs of wireless charging models, dual coil charging lines, dual charging frequencies, and coupling coefficients as well as the influence of the coil on charging. Based on the review of the different designs, it was found that the dual coil design can improve the charging efficiency in terms of quantity. The dual-charging frequency design improves the versatility of wireless charging. Variations in the number of turns and coupling coefficients of the coils can improve the efficiency of the transmission.

Co-Expression of ZmVPP1 with ZmNAC111 Confers Robust Drought Resistance in Maize.

Drought is a primary environmental factor limiting maize production globally. Although transferring a single gene to maize can enhance drought resistance, maize response to water deficit requires further improvement to accommodate the steadily intensifying drought events worldwide. Here, we generated dual transgene lines simultaneously overexpressing two drought-resistant genes, ZmVPP1 (encoding a vacuolar-type H+ pyrophosphatase) and ZmNAC111 (encoding a NAM, ATAF, and CUC (NAC)-type transcription factor). Following drought stress, survival rates of the pyramided transgenic seedlings reached 62-66%, while wild-type and single transgene seedling survival rates were 23% and 37-42%, respectively. Maize seedlings co-expressing ZmVPP1 and ZmNAC111 exhibited higher photosynthesis rates, antioxidant enzyme activities, and root-shoot ratios than the wild type, and anthesis-silking intervals were shorter while grain yields were higher under water deficit conditions in field trials. Additionally, RNA-sequencing analysis confirmed that photosynthesis and stress-related metabolic processes were stimulated in the dual transgene plants under drought conditions. The findings in this work illustrate how high co-expression of different drought-related genes can reinforce drought resistance over that of individual transgene lines, providing a path for developing arid climate-adapted elite maize varieties.

Study of structural effects on the polarization characteristics of subwavelength metallic gratings in short infrared wavelengths.

Applications of subwavelength grating based-polarizers for polarimetric detections are being hindered due to the limited extinction ratio. In this work, the structural effect, including the line edge roughness (LER), of the gratings on the polarizing characteristics was studied by both numerical simulations using finite difference and time domain (FDTD) method and experiments, aiming to figure out the optimal grating profile for achieving high transmittance as well as high extinction ratio. Two different configurations of the gratings, one is dual layer Au lines and the other is parabolic shaped Al lines on structured spin-on-carbon (SOC) films were systematically studied and compared. Nanofabrication of the gratings by electron beam lithography without lift-off process were conducted and optical measurements of their polarization properties demonstrate superior performance of the developed polarizers. The origin of the structural effect was explained by the local surface plasmonic modes, existing in the nano-slits in metallic gratings, which is instructive for further enhancement of the polarization performance.

Specific lateral flow detection of isothermal nucleic acid amplicons for accurate point-of-care testing

For point-of-care testing (POCT), coupling isothermal nucleic acid amplification schemes (e.g., recombinase polymerase amplification, RPA) with lateral flow assay (LFA) readout is an ideal platform, since such integration offers both high sensitivity and deployability. However, isothermal schemes typically suffers from non-specific amplification, which is difficult to be differentiated by LFA and thus results in false-positives. Here, we proposed an accurate POCT platform by specific recognition of target amplicons with peptide nucleic acid (PNA, assisted by T7 Exonuclease), which could be directly plugged into the existing RPA kits and commercial LFA test strips. With SARS-CoV-2 as the model, the proposed method (RPA-TeaPNA-LFA) efficiently eliminated the false-positives, exhibiting a lowest detection concentration of 6.7 copies/μL of RNA and 90 copies/μL of virus. Using dual-gene (orf1ab and N genes of SARS-CoV-2) as the targets, RPA-TeaPNA-LFA offered a high specificity (100%) and sensitivity (RT-PCR Ct < 31, 100%; Ct < 40, 71.4%), and is valuable for on-site screening or self-testing during isolation. In addition, the dual test lines in the test strips were successfully explored for simultaneous detection of SARS-CoV-2 and H1N1, showing great potential in response to future pathogen-based pandemics.

Complete Frequency-Bin Bell Basis Synthesizer

We report the experimental generation of all four frequency-bin Bell states in a single versatile setup via successive pumping of spontaneous parametric down-conversion with single and dual spectral lines. Our scheme utilizes intensity modulation to control the pump configuration and offers turn-key generation of any desired Bell state using only off-the-shelf telecommunication equipment. We employ Bayesian inference to reconstruct the density matrices of the generated Bell states, finding fidelities ≥97% for all cases. Additionally, we demonstrate the sensitivity of the frequency-bin Bell states to common-mode and differential-mode temporal delays traversed by the photons comprising the state-presenting the potential for either enhanced resolution or nonlocal sensing enabled by our complete Bell basis synthesizer.

Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242

Genetically encoded voltage indicators (GEVIs) allow for monitoring membrane potential changes in neurons and cardiomyocytes (CMs) as an alternative to patch-clamp techniques. GEVIs facilitate non-invasive, high throughput screening of electrophysiological properties of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). A dual transgenic hiPSC line with Arclight A242 (GEVI) and an antibiotic resistance cardiac selection cassette was successfully generated from an earlier established hiPSC line MHHi001-A. After cardiac differentiation and selection, purified populations of CMs with constitutive GEVI expression can be utilized for studying cardiac development, disease modeling, and drug testing.

The Prospect of Using the Dual Gauge Line for the Ukraine–Hungary Railway Connection

There are several international transport corridors in Hungary. Záhony railway station is one of the largest hubs in Europe, providing a railway connection between Ukraine and the European Union. Two different railway tracks meet and come together here, such as the standard (1435 mm) and broad (1520 mm) gauges. The availability of a developed infrastructure of the dual gauge on the territory of Hungary presupposes the corresponding development of the railway connection by Ukraine. In order to effectively use the dual gauge line and solve problems of special design, it is necessary to ensure the appropriate train flows. This research aims to provide scientific support for express analysis of the railway routes' competitiveness between Ukraine and the European Union to define the determining factors. The application of such approaches provides a tool for establishing the prospects for the development of existing railway lines, considering their features. Scientific approaches to creating methods for determining the priority areas of railway transport have been further developed. Apart from involving such essential indices as average speed and traffic volumes, the authors added the possibility of considering the presence of lines with such design features as single-track sections, non-electrified, dual gauge, etc., on the route. The theoretical background is applied as a tool for rapid calculations for increasing the competitiveness of the Chop–Záhony dual gauge line.