Plant diseases caused by pathogenic fungi result in substantial losses of human, material, and financial resources annually. The common practice of chemical control often leads to the development of drug resistance in target organisms and carries adverse environmental impacts. As an alternative, researchers are exploring the potential of microbial control agents found in the rhizosphere.Previous studies have identified numerous promising microbial strains that could be utilized as effective, ecologically-friendly, and sustainable solutions to manage plant diseases. In the present investigation, a bacterial strain designated QF-2 was isolated from the rhizosphere soil of Vickifunkia tianschanica. Through dual culture experiments and molecular biological analysis, the taxonomic status of this strain was clearly established, and its antagonistic activities against seven plant pathogens were observed. Furthermore, the researchers conducted a comprehensive analysis of the QF-2 genome. Concurrently, five secondary metabolites (1–5) produced by QF-2 were studied, isolated, and identified.