DSP Data Research Articles

Spatial transcriptome profiling identifies DTX3L and BST2 as key biomarkers in esophageal squamous cell carcinoma tumorigenesis

BackgroundUnderstanding the stepwise progression of esophageal squamous cell carcinoma (ESCC) is crucial for developing customized strategies for early detection and optimal clinical management. Herein, we aimed to unravel the transcriptional and immunologic alterations occurring during malignant transformation and identify clinically significant biomarkers of ESCC.MethodsDigital spatial profiling (DSP) was performed on 11 patients with early-stage ESCC (pT1) to explore the transcriptional alterations in epithelial, immune cell, and non-immune cell stromal compartments across regions of distinct histology, including normal tissues, low- and high-grade dysplasia, and cancerous tissues. Furthermore, single-cell spatial transcriptomics was performed using the CosMx Spatial Molecular Imaging (SMI) system on 4 additional patients with pT1 ESCC. Immunohistochemical (IHC) analysis was performed on consecutive histological sections of 20 pT1 ESCCs. Additionally, public bulk and single-cell RNA-sequencing (scRNA-seq) datasets were analyzed, and in vitro and in vivo functional studies were conducted.ResultsSpatial transcriptional reprogramming and dynamic cell signaling pathways that determined ESCC progression were delineated. Increased infiltration of macrophages from normal tissues through dysplasia to cancerous tissues occurred. Macrophage subtypes were characterized using the scRNA-seq dataset. Cell–cell communication analysis of scRNA-seq and SMI data indicated that the migration inhibitory factor (MIF)-CD74 axis may exhibit pro-tumor interactions between macrophages and epithelial cells. DSP, SMI, and IHC data demonstrated that DTX3L expression in epithelial cells and BST2 expression in stromal cells increased gradually with ESCC progression. Functional studies demonstrated that DTX3L or BST2 knockdown inhibited ESCC proliferation and migration and decreased M2 polarization of tumor-associated macrophages.ConclusionsSpatial profiling comprehensively characterized the molecular and immunological hallmarks from normal tissue to ESCC, guiding the way to a deeper understanding of the tumorigenesis and progression of this disease and contributing to the prevention of ESCC. Within this exploration, we uncovered biomarkers that exhibit a robust correlation with ESCC progression, offering potential new avenues for insightful therapeutic approaches.

The status of violence against children in China, 2013-2021

Objective: This study aims to obtain the prevalence and features associated with Violence Against Children (VAC) in China and, thus, formulate a prevention strategy. Methods: The mortality-related data of VAC was sourced from the National Disease Surveillance Points System (DSP) during 2013-2021. We analyzed the DSP data regarding children aged 0-17 years old who died from violence. The hospital cases of VAC was sourced from the National Injury Surveillance System (NISS), 2013-2021. We analyzed the data from NISS with the parameter of "intentional injury" caused by VAC in children aged between 0-17 years. Using robust linear regression, we analyze the time trend in the proportion of violence incidence. To understand the variations in the incidence of different types of violence across genders, we apply the chi-square test and adjusted Pearson residuals. Results: The overall trend of death caused by VAC has declined; it was reduced to 0.14/100 000 in 2021 from 0.33/100 000 in 2013. In 2021, male VAC mortality (0.15/100 000) was higher than females (0.13/100 000). The proportion of VAC cases to all injury cases has declined from 3.34% in 2013 to 2.29% in 2021. Among 9 344 VAC cases supervised by hospitals in 2021, the number of males (7 503 cases) was around 4 times that of females (1 841 cases), and the top three modes of violence were blunt tools (64.77%), falls (7.46%) and sharp instruments (6.18%), and 45 cases of sexual violence included 38 girls and 7 boys. Conclusions: The declining death rate due to VAC may be related to the benign development of Chinese society. Prevention strategies targeting training in parenting skills and problem-solving should be prioritized.

Development of a DSP based collaborative computing platform for power system

Abstract With the advancement of information technology, there has been tremendous progress in data production, processing, and storage technology, especially in data processing technology. With the rapid development of the power grid, its operation has become more intelligent and efficient. Traditional power system simulation and analysis tools have disadvantages, such as slow message transmission and the inability to share data, which cannot meet the requirements of modern power system simulation calculations. DSP was introduced and developed by the Southern Power Grid Research Institute and can perform various simulation calculations such as transient stability calculation, power flow calculation, and short circuit calculation. It is widely used in departments such as power planning and design. However, the data calculated by DSP is input through a data card, which is cumbersome and difficult to manage. In order to meet the new requirements of power system simulation analysis, a power system collaborative computing platform has been developed based on DSP, which makes DSP data maintenance and management more efficient and intuitive. It comprehensively improves the efficiency of power grid planning work. This article provides a detailed analysis of the architecture design and application problems of the power system collaborative computing platform, clarifies the requirements of large-scale interconnected power grids for simulation computing data management, and proposes solutions that can meet the needs of simulation computing data management in China’s power grid from two aspects: key data architecture issues and software architecture technology.

Spatial Proteome Analysis Identifies Lymphocyte CD44 as a Biomarker Associated with SBRT Resistance in Early-Stage Non-Small Cell Lung Cancer

To discover and validate spatially-resolved protein markers associated with resistance to SBRT in early-stage NSCLC patients. We initially evaluated a discovery cohort of 44 early-stage NSCLC patients treated with SBRT as first-line treatment at the Shandong cancer hospital. Using the GeoMx DSP system, 71 proteins were measured in five molecular compartments (tumor, leukocyte, lymphocyte, macrophage, and stroma) on pre-treatment samples. Candidate biomarkers were orthogonally validated with the Gem AQUA method of quantitative immunofluorescence (QIF). For internal independent cohort validation, we assessed pre-treatment samples derived from 150 NSCLC patients who receive radiotherapy. We further analyzed 100 radiotherapy untreated patients with operable NSCLC to address prognostic significance. Using continuous log-scaled data, we identified CD44 expression in the lymphocyte compartment (CD3+) as a novel predictor of poor progression-free survival (PFS) (multivariate HR = 7.323, p = 0.0079) and overall survival (OS) (multivariate HR = 8.65, p = 0.028) in the discovery set. High CD44 expression in the tumor compartment (pan-cytokeratin, CK+) predicted significantly shorter OS (multivariate HR = 2.208, p = 0.0212), with no significant difference in PFS. We validated by QIF that lymphocyte CD44 levels were associated with resistance to SBRT therapy and prognostic for poor outcomes. Using QIF in an independent radiotherapy treated cohort, we validated that CD44 levels in the lymphocyte compartment were associated with poor PFS and OS. High lymphocyte cell CD44 was not prognostic in non-radiotherapy-treated cohort. Using DSP data, intratumoral regions with elevated lymphocyte cell CD44 expression showed prominent upregulation of CD127, ARG1 and VISTA in the discovery Cohort. In conclusion, we identified and validated lymphocyte cell CD44 as a biomarker indicative of resistance to SBRT or radiotherapy in patients with NSCLC. Further evaluation is warranted to address the predictive value of lymphocyte cell CD44 in multi-institutional studies and clinical trials.

Abstract PS16-01: Intra-epithelial tumor immune landscapes are associated with clinical outcomes in early-stage triple-negative breast cancer

Abstract Introduction: Stromal tumor-infiltrating lymphocytes (sTILs) have established prognostic and predictive significance in triple-negative breast cancer (TNBC). However, the roles of other immune cells in TNBC are less well-established. We performed high-plex quantitative spatial profiling in a cohort of early-stage TNBC to 1) apply spatial context to tumoral immune landscapes and 2) identify immune proteins associated with clinical outcomes, independently of TILs and other established prognostic clinicopathologic variables, in patients (pts) treated with or without adjuvant chemotherapy (CTX). Methods: The Mayo TNBC cohort comprises pts with centrally-verified, CTX-naive tumors resected from 1985-2012. Using a cohort-based TMA, with Nanostring GeoMX DSP, we quantitated 58 proteins within spatially-distinct intra-epithelial, cytokeratin-positive tumor segments and adjacent cytokeratin-negative/nuclei-positive stromal segments. Differentially-expressed (DE) proteins were identified using a negative binomial generalized linear model (SNR>2, p< 0.05) and a target DE protein set was dichotomized (80th percentile). After adjusting for prognostic clinicopathologic variables, proteins associated with recurrence-free survival (RFS, defined as time from surgery to either local, regional, and distant recurrence, or death by any cause) were identified by performing variable selection using the Akaike Information Criterion (AIC) obtained from fitting all possible Cox proportional hazards regression models (performed separately for intra-epithelial/stromal segments, and in groups +/- adjuvant CTX. Results: From the TNBC TMA, DSP data (N=250 tumors) included 169 pts who received adjuvant CTX+ and 81 who did not (CTX-). Overall, 85/250 developed recurrent disease. In the CTX+ group, intra-epithelial tumor segments from pts without recurrent disease were enriched in 10 immune proteins, including CD8, markers involved in antigen presentation/dendritic cells (CD11c, CD40, HLA-DR) or NK cells (CD56) (FC: 1.4-2.1, p<0.05); CD14 was increased in stroma (FC: 1.5, p<0.05). In contrast, in the CTX- group, both the intra-epithelial tumor and stromal segments from pts without recurrences were enriched in immune proteins (N= 12 and 15 respectively; FC 1.6-5.5, p< 0.05) most markedly CD40, IDO1 and HLA-DR (FC: 3.2-5.5, p< 0.05). Overall, CD3, CD4, CD27, CD44, and ICOS among others were enriched only in the CTX- group; CD14 and CD56 were enriched only in the CTX+ group. Based on these spatial data, biologic function and DSP data from another set of TNBC (FinXX trial), CD11c, CD14, CD27, CD40, CD56, and IDO1 were selected for RFS analysis. After applying our model selection criterion and adjusting for pt age at surgery, tumor size, lymph node status, and sTILs, intra-epithelial CD56 was independently associated with improved RFS in the CTX+ group (HR: 0.31[0.12, 0.81]). In the CTX- group, intra-epithelial CD11c was independently associated with improved RFS (0.10 [0.01, 0.81]). Conclusion: In this early-stage TNBC cohort, spatially-distinct tumor immune landscapes were associated with RFS but differed according to receipt of CTX after surgical resection. In the patients who received CTX, the intra-epithelial compartment, rather than stromal compartment, was immune-enriched in pts without recurrences. Among a targeted protein set, intra-epithelial CD56 remained associated with improved outcomes, independent of sTILs and other clinicopathologic features. In the CTX- group, spatial landscapes were more balanced, and intra-epithelial CD11c was independently associated with improved outcomes. These data provide insight into the spatial context of intrinsic immune landscapes in TNBC, and identify candidate prognostic immune biomarkers which may inform therapeutic strategies. Citation Format: Jodi M Carter, Saranya Chumsri, David W Hillman, David M Zahrieh, Yaohua Ma, Xue Wang, Jennifer M Kachergus, Judy C Boughey, Minetta C Liu, Krishna R Kalari, JC Villasboas, Roberto A Leon Ferre, Fergus J Couch, Matthew P Goetz, E. Aubrey Thompson. Intra-epithelial tumor immune landscapes are associated with clinical outcomes in early-stage triple-negative breast cancer [abstract]. In: Proceedings of the 2020 San Antonio Breast Cancer Virtual Symposium; 2020 Dec 8-11; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2021;81(4 Suppl):Abstract nr PS16-01.

Design of multi-channel analog acquisition system based on DSP + FPGA architecture

This paper proposes a design and implementation of a general multi-channel analog acquisition system based on DSP + FPGA architecture. In terms of hardware architecture, FPGA is used to achieve high-speed and high reliability switching between different analog channels; FPGA + DSP data processing architecture is used to separate data acquisition and algorithm processing.

Design and Implementation of DSP Based Cross-Platform Material Scratch Test Control System

Scratch test is mainly used for quantitative measurement of mechanical properties of coating materials in the field of modern materials testing. Advanced materials research have higher accuracy and stability requirements for scratch device but the control system the device mainly use can’t achieve dynamic and fast scratch because of the slow feedback. This paper designed a cross-platform material scratch test control system based on DSP data transmission and multi-axes control technique. System integrates good dynamic response sensors and advanced piezoelectric ceramic feed system, implements multi-axis synchronous control through the motion controller and running state monitoring through the DSP module .Adopting the standard PCI interface and cross-platform software development, the system has good portability.

Propensity score weighting for addressing under-reporting in mortality surveillance: a proof-of-concept study using the nationally representative mortality data in China.

BackgroundNational mortality data are obtained routinely by the Disease Surveillance Points system (DSPs) in China and under-reporting is a big challenge in mortality surveillance.MethodsWe carried out an under-reporting field survey in all 161 DSP sites to collect death cases during 2009–2011, using a multi-stage stratified sampling. To identify under-reporting, death data were matched between field survey system and the routine online surveillance system by an automatic computer checking followed by a thorough manual verification. We used a propensity score (PS) weighting method based on a logistic regression to calculate the under-reporting rate in different groups classified by age, gender, urban/rural residency, geographic locations and other mortality related variables. For comparison purposes, we also calculated the under-reporting rate by using capture-mark-recapture (CMR) method.ResultsThere were no significant differences between the field survey system and routine online surveillance system in terms of age group, causes of death, highest level of diagnosis and diagnostic basis. The overall under-reporting rate in the DSPs was 12.9 % (95%CI 11.2 %, 14.6 %) based on PS. The under-reporting rate was higher in the west (18.8 %, 95%CI 16.5 %, 21.0 %) than the east (10.1 %, 95%CI 8.6 %, 11.3 %) and central regions (11.2 %, 95%CI 9.6 %, 12.7 %). Among all age groups, the under-reporting rate was highest in the 0–5 year group (23.7 %, 95%CI 16.1 %, 35.5 %) and lowest in the 65 years and above group (12.4 %, 95%CI 10.9 %, 13.6 %). The under-reporting rates in each group by PS were similar to the results calculated by the CMR methods.ConclusionsThe mortality data from the DSP system in China needs to be adjusted. Compared to the commonly used CMR method in the estimation of under-reporting rate, the results of propensity score weighting method are similar but more flexible when calculating the under-reporting rates in different groups. Propensity score weighting is suitable to adjust DSP data and can be used to address under-reporting in mortality surveillance in China.

The implementation of FPGA for data transmission between ADC and DSP peripherals in the measurement channels for power quality assessment

The paper presents the FPGA Xilinx Spartan 3 series implementation in an analog data acquisition system, in particular consisting of nine ADCs and the TigerSHARC DSP processor. Because of different standards of ADC and DSP data interfaces, the exchange of data between them requires the use of an intermediary device for combining their communication ports. The procedures for sending data from the ADC to the FPGA buffers, their conversion and subsequent transmission to the TigerSHARC DSP are described. The digital representations of ADC analog input signals are sent to the FPGA through three lines of serial interface at the physical layer compatible with the SPI standard. After processing in the FPGA they are sent to the DSP, with the four-line interface in the LVDS standard. The paper discusses the properties of related interfaces, standards, procedures and control of ADC issues as well as the algorithms of the FPGA configuration program and functions implemented in this system. Selected results of functional testing are shown.

SHSD Demodulator of Interferometric Integrated Optical Acceserometer Based on SOPC

It is described that the acceleration signal is modulated to optical phase by Micheson interference device and demodulated by Synthetic-heterodyne signal demodulation(SHSD) ,when the error is compensated by alternating current phase tracking homodyne compensation scheme (PTAC). Then the digital signal proseccing system is designed with SOPC technology. And DSP and intelligent data communication interface run in parallel . The experiment has shown that when the virtual value of external acceleration signal is constant, in selected working frequency range, the system output signal can trace the vibration acceleration signal linearly and reliably.

Crystal Structure of the Cysteine-rich Domain of Scavenger Receptor MARCO Reveals the Presence of a Basic and an Acidic Cluster That Both Contribute to Ligand Recognition

MARCO is a trimeric class A scavenger receptor of macrophages and dendritic cells that recognizes polyanionic particles and pathogens. The distal, scavenger receptor cysteine-rich (SRCR) domain of the extracellular part of this receptor has been implicated in ligand binding. To provide a structural basis for understanding the ligand-binding mechanisms of MARCO, we have determined the crystal structure of the mouse MARCO SRCR domain. The recombinant SRCR domain purified as monomeric and dimeric forms, and their structures were determined at 1.78 and 1.77 A resolution, respectively. The monomer has a compact globular fold with a twisted five-stranded antiparallel beta-sheet and a long loop covering a single alpha-helix, whereas the dimer is formed via beta-strand swapping of two monomers, thus containing a large eight-stranded beta-sheet. Calculation of the surface electrostatic potential revealed that the beta-sheet region with several arginines forms a basic cluster. Unexpectedly, an acidic cluster was found in the long loop region. In the monomer, the acidic cluster is involved in metal ion binding. Studies with cells expressing various SRCR domain mutants showed that all of the arginines of the basic cluster are involved in ligand binding, suggesting a cooperative binding mechanism. Ligand binding is also dependent on the acidic cluster and Ca2+ ions whose depletion appears to affect ligand binding at least by modulating the electrostatic potential or relative domain orientation. We propose that the SRCR domain dimerization can contribute to the recognition of large ligands by providing a means for the MARCO receptor oligomerization.

A comparison of the sources of cancer mortality in China.

To compare the validity of mortality data from available sources in China. Two large-scale surveys have provided accurate national-level rates; the most recent involved deaths occurring in a random 10% sample of the population during 1990-1992. Since then, the only readily available sources are two on-going surveillance systems, which provide annual estimates of mortality--the "Disease Surveillance Points" (DSP) sample survey, and that established by the Center of Health Information and Statistics (CHIS) of the ministry of health, the results of which are published by WHO. They were compared with respect to the representativeness of the populations covered and the rates obtained. Neither source covers a random sample of the Chinese population, with respect to age group, sex, and urban-rural residence, although the DSP population is the more representative of the national population in this respect. Sex and region (urban/rural) specific age-standardized mortality rates from the CHIS dataset were, however, closer to those from the (1990-1992) national survey, than those calculated from DSP data. The CHIS data is the preferred source for estimation of national mortality, and study of time trends, but requires appropriate weighting (by age, sex, rural/urban residence). The within-stratum estimates are more stable than those of DSP, because of its larger sample size.

Software defined QCIF simple profile MPEG-4 for portable devices using dynamically reconfigurable DSP

The ISO MPEG-4 standard is emerging as the dominant digital video standard, and efficient, programmable MPEG-4 video codecs are paramount for emerging mobile devices that aggregate telecommunications with features including digital video. Efficient QCIF simple profile MPEG-4 video codec algorithms are developed for typical 16-bit, dual execution unit DSPs and the additional impact of tightly coupled, co-processors used to dynamically reconfigure the DSP data path is quantified. Efficient, programmable solutions consuming approximately 63 Mcycles, significantly lower than estimates as high as 400 Mcycles, are generated, while dynamic reconfiguration reduces this cost an additional 20%.

A reconfigurable multiprocessor IC for rapid prototyping of algorithmic-specific high-speed DSP data paths

A field-programmable multiprocessor integrated circuit, PADDI (programmable arithmetic devices for high-speed digital signal processing), has been designed for the rapid prototyping of high-speed data paths typical to real-time digital signal processing applications. The processor architecture addresses the key requirements of these data paths: (a) fast, concurrently operating, multiple arithmetic units, (b) conflict-free data routing, (c) moderate hardware multiplexing (of the arithmetic units), (d) minimal branch penalty between loop iterations, (e) wide instruction bandwidth, and (f) wide I/O bandwidth. The initial version contains eight processors connected via a dynamically controlled crossbar switch, and has a die size of 8.9*9.5 mm in a 1.2- mu m CMOS technology. With a maximum clock rate of 25 MHz, it can support a computation rate of 200 MIPS and can sustain a data I/O bandwidth of 400 Mbytes/s with a typical power consumption of 0.45 W. An assembler and simulator have been developed to facilitate programming and testing of the chip.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>

The Relationship of Biopsy Evaluations and Testicular Measurements to Over-All Daily Sperm Production in Human Testes

Enumeration of homogenization-resistant spermatids in testicular homogenates provided a rapid and precise means of characterizing human spermatogenesis in quantitative terms. This technique was applied to simulated biopsy specimens as small as 10 mg of testicular parenchyma and yielded values for daily sperm production per gram of testicular parenchyma (DSP/gm) that were highly correlated with over-all DSP/gm values for paired testes (r = +0.86) and DSP/pair of testes (r = +0.93). In 12 autopsy cases representing a 100-fold range of sperm production rates, the average difference between DSP/gm values obtained from simulated biopsy specimens and from entire testes was not significantly different from zero (P < 0.05). The small amount of testicular parenchyma required for this determination indicated that both DSP data and routine histologic observations can be collected from the material included in a conventional testicular biopsy. Various measurements related to testicular size were positively but weakly correlated with DSP/testis (values for r ranged from +0.45 to +0.66). Hence, testicular size was a relatively poor index of sperm production in this study.