Drying-rewetting Cycles Research Articles

Drying-rewetting alters arsenic ecotoxicity: From the perspective of enzyme-based functional diversity

Drying-rewetting (DW) cycles can significantly influence soil properties and microbial community composition, leading to direct or indirect changes in arsenic (As) toxicity, which inturn affects soil ecological functions. Despite this, there has been insufficient focus on accurately evaluating As ecotoxicity and its impact on soil ecological function under DW conditions. This study seeks to address this gap by examining the effects of DW on As toxicity and the characteristics of soil ecological function, specifically from the perspective of enzyme-based functional diversity. Our results reveal that compared to constant moisture conditions, DW treatment significantly increased the toxicity of As on alkaline phosphatase and β-glucosidase, with maximum inhibition rates observed at 46.29% and 21.54%, respectively. Conversely, for other tested enzymes including invertase, fluorescein diacetate hydrolase, and dehydrogenase, DW treatment decreased As toxicity, possibly be due to the different stability of these enzymes under varying soil moisture conditions. From an enzyme functional diversity perspective, DW treatment reduced the As toxicity, as evidenced by the reduced inhibition rates and a lower coefficient of variation. In conclusion, DW appears to enhance soil functional resilience against arsenic pollution. These findings contribute to a better understanding of changes in ecological functions in heavy metal-contaminated soils under dynamic environmental conditions, offering insights for improved monitoring and mitigation strategies for metalloids toxicity in natural environments.

Orthophosphate uptake onto woodchips in denitrifying bioreactors is enhanced by anoxic-(anaerobic-)oxic cycling

Woodchips are widely used as a low-cost and renewable organic carbon source for denitrifying biofilms in passive nutrient removal systems. One limitation of wood-based biofiltration systems is their relatively poor removal of phosphorus (P) from subsurface drainage and stormwaters, necessitating the use of additional filter media when co-treatment of nitrogen (N) and P is required. Here, we show that anoxic-oxic cycling of woodchip media, which enhances nitrate (NO3−) removal by increasing the mobilization of organic carbon from wood, also improves orthophosphate (Pi) uptake onto woodchips. Orthophosphate removal rates in flow-through woodchip columns ranged from 0 to 34.9 μg PO43− L−1 h−1 under continuously-saturated (anoxic) conditions, and increased to 17.5 to 71.9 μg PO43− L−1 h−1 in columns undergoing drying-rewetting (oxic-anoxic) cycles. The highest Pi removal efficiencies were observed in the first 20 h after reactors were re-flooded, and were concurrent with maxima in polyphosphate kinase (ppk) gene expression by the polyphosphate accumulating organisms (PAOs) Accumulibacter spp. and Pseudomonas spp. Batch experiments confirmed that anoxic-anaerobic-oxic pre-incubation conditions led to orthophosphate uptake onto woodchips as high as 74.9 ± 0.8 mg PO43−/kg woodchip, and batch tests with autoclaved woodchips demonstrated that Pi uptake was due to biological processes and not adsorption. NO3− removal in batch tests was also greatest under oxic incubation conditions, attributed to greater carbon availability in hypoxic to anoxic zones in woodchip biofilms. While further research is needed to elucidate the mechanisms controlling enhanced Pi uptake by woodchip biofilms under anoxic-(anaerobic-)oxic cycling, these results suggest a role for enhanced Pi uptake by PAOs in a nature-based system for treatment of nonpoint source nutrients.

Drying and rewetting affect the chemical speciation and bioavailability of soil phosphorus in a hyper-arid desert ecosystem

Climate change is expected to alter the frequency and intensity of drying and rewetting cycles, impacting water availability and consequently soil nutrient availability. However, the effects of these fluctuations on the chemical speciation and bioavailability of phosphorus (P) in soil with or without desert species remain uncertain. We conducted a pot experiment involving bare soil and two desert species (Alhagi sparsifolia and Calligonum mongolicum) to determine the short-term impacts of drought (no water supply), drying-rewetting-1 (D-RW1, high frequency of low water amounts), and drying-rewetting-2 (D-RW2, low frequency of high-water amounts) treatments on soil Hedley-P pools, plant P concentration and biomass accumulation. Results demonstrated that the presence of plants significantly increased soil labile-P and organic P (Po) concentrations by 60–150% and 1–68%, respectively, compared to bare soil. Both D-RW1 and D-RW2 treatments significantly increased soil dissolved organic carbon concentration by 2–35% relative to the drought treatment. Moreover, soil resin-P and NaHCO3-Pi concentrations under Alhagi sparsifolia in D-RW1 treatment significantly increased by 31% and 75%, respectively, when compared to the drought treatment, with NaHCO3-Po and NaOH-Po concentrations under D-RW2 treatment rising by 14% and 32%, respectively. Furthermore, D-RW2 treatment significantly increased leaf P concentration and biomass compared to D-RW1 and drought treatments. Overall, compared to drought treatment, frequent low-intensity drying-rewetting cycles enhanced soil Pi turnover, whereas infrequent high-intensity drying-rewetting cycles increased Po turnover and P bioavailability. These findings will inform better water management strategies for desertification restoration in hyper-arid desert ecosystems.

Straw application improved soil biological properties and the growth of rice plant under low water irrigation

The application of organic amendments is one way to manage low water irrigation in paddy soils. In this 60-day greenhouse pot experiment involving paddy soil undergoing drying-rewetting cycles, we examined the effects of two organic amendments: azo-compost with a low carbon to phosphorus ratio (C:P) of 40 and rice straw with a high C:P ratio of 202. Both were applied at rates of 1.5% of soil weight (w/w). The investigation focused on changes in certain soil biochemical characteristics related to C and P in the rice rhizosphere, as well as rice plant characteristics. The irrigation regimes applied in this study included constant soil moisture in a waterlogged state (130% water holding capacity (WHC)), mild drying–rewetting (from 130 to 100% WHC), and severe drying–rewetting (from 130 to 70% WHC). The results indicated that the application of amendments was effective in severe drying–rewetting irrigation regimes on soil characteristics. Drying–rewetting decreased soil respiration rate (by 60%), microbial biomass carbon (by 70%), C:P ratio (by 12%), soil organic P (by 16%), shoot P concentration (by 7%), and rice shoot biomass (by 30%). However, organic amendments increased soil respiration rate (by 8 times), soil microbial biomass C (51%), total C (TC) (53%), dissolved organic carbon (3 times), soil available P (AP) (100%), soil organic P (63%), microbial biomass P (4.5 times), and shoot P concentration (21%). The highest significant correlation was observed between dissolved organic carbon and total C (r= 0.89**). Organic amendments also increased P uptake by the rice plant in the order: azo-compost > rice straw > control treatments, respectively, and eliminated the undesirable effect of mild drying–rewetting irrigation regime on rice plant biomass. Overall, using suitable organic amendments proves promising for enhancing soil properties and rice growth under drying–rewetting conditions, highlighting the interdependence of P and C biochemical changes in the rhizosphere during the rice vegetative stage.

Systematic evaluation of methods for iron-impregnation of biochar and effects on arsenic in flooded soils.

There is a need for innovative strategies to decrease the mobility of metal(loids) including arsenic (As) and cadmium (Cd) in agricultural soils, including rice paddies, so as to minimize dietary exposure to these toxic elements. Iron (Fe)-modified biochars (FBCs) are used to immobilize As and Cd in soil-water systems, but there is a lack of clarity on optimal methods for preparing FBCs because there are only limited studies that directly compare BCs impregnated with Fe under different conditions. There is also a lack of information on the long-term performance of FBCs in flooded soil environments, where reductive dissolution of Fe (oxy)hydroxide phases loaded onto biochar surfaces may decrease the effectiveness of FBCs. This study uses material characterization methods including FTIR, SEM-EDX, BET, and adsorption isotherm experiments to investigate the effects of Fe-impregnation methods (pH, pyrolysis sequence, and sonication) on the morphology and mineralogy of Fe loaded onto the biochar surface, and to FBC adsorbent properties for arsenate (As(V)), arsenite (As(III)), and Cd. Acidic impregnation conditions favored the adsorption of As(III) onto amorphous Fe phases that were evenly distributed on the biochar surface, including within the biochar pore structure. The combination of sonication with acidic Fe-impregnation conditions led to the best adsorption capacities for As(V) and As(III) (4830 and 11,166 μg As g-1 biochar, respectively). Alkaline Fe-impregnation conditions led to the highest Cd adsorption capacity of 3054 μg Cd g-1 biochar, but had poor effectiveness as an As adsorbent. Amending soil with 5% (w/w) of an acid-impregnated and sonicated FBC was more effective than an alkaline-impregnated FBC or ferrihydrite in decreasing porewater As concentrations. The acid-impregnated FBC also had greater longevity, decreasing As by 54% and 56% in two flooded phases, probably due to the greater stability of Fe(III) within the biochar pore structure that may have a direct chemical bond to the biochar surface. This study demonstrates that FBCs can be designed with selectivity towards different As species or Cd and that they can maintain their effectiveness under anaerobic soil conditions. This is the first study to systematically test how impregnation conditions affect the stability of FBCs in soils under multiple drying-rewetting cycles.

Cadmium concentrations in pore waters can largely increase during soil incubation: Artefacts with consequences for Cd limits in fertilisers

A sound evaluation of the cadmium (Cd) mass balance in agricultural soils needs accurate data of Cd leaching. Reported Cd concentrations from in situ studies are often one order of magnitude lower than predicted by empirical models, which were calibrated to pore water data from stored soils. It is hypothesized that this discrepancy is related to the preferential flow of water (non-equilibrium) and/or artefacts caused by drying and rewetting soils prior to pore water analysis. These hypotheses were tested on multiple soils (n = 27) with contrasting properties. Pore waters were collected by soil centrifugation from field fresh soil samples and also after incubating the same soils (28 days, 20 °C), following two drying-rewetting cycles, the idea being that chemical equilibrium in the soil is reached after incubation. Incubation increased pore water Cd by a factor 4, on average, and up to a factor 16. That increase was statistically related to the decrease of pore water pH and the increase of nitrate, both mainly related to incubation-induced nitrification. After correcting for both factors, the Cd rise was also highest at higher pore water Ca. This suggests that higher Ca in soil enlarges Cd concentration gradients among pore classes in field fresh soils because high Ca promotes soil aggregation and separation of mobile from immobile water. Several empirical models were used to predict pore water Cd. Predictions exceeded observations up to a factor 30 for the fresh pore waters but matched well with those of incubated soils; again, deviations from the 1:1 line in field fresh soils were largest in high Ca (>0.8 mM) soils, suggesting that local equilibrium conditions in field fresh soils are not found at higher Ca. Our results demonstrate that empirical models need recalibration with field fresh pore water data to make accurate soil Cd mass balances in risk assessments.

High soil moisture rather than drying-rewetting cycles reduces the effectiveness of nitrification inhibitors in mitigating N2O emissions

Climate change has been intensifying soil drying and rewetting cycles, which can alter the soil microbiome structure and activity. Here we hypothesized that a soil drying-rewetting cycle enhances biodegradation and, hence, decreases the effectiveness of nitrification inhibitors (NIs). The effectiveness of DMPP (3,4-Dimethylpyrazole phosphate) and MP + TZ (3-Methylpyrazol and Triazol) was evaluated in 60-day incubation studies under a drying and rewetting cycle relative to constant low and high soil moisture conditions (40% and 80% water-holding capacity, WHC, respectively) in two different textured soils. The measurements included (i) daily and cumulative N2O-N emissions, (ii) soil NH4+-N and NO3−-N concentrations, and (iii) the composition of bacterial soil communities. Application of DMPP and MP + TZ reduced the overall N2O-N emissions under drying-rewetting (-45%), as well as under 40% WHC (-39%) and 80% WHC (-25%). DMPP retarded nitrification and decreased N2O-N release from the sandy and silt loam soils, while MP + TZ mitigated N2O-N production only from the silt loam soil. Unexpectedly, between days 30 and 60, N2O-N emissions from NI-treated soils increased by up to fivefold relative to the No-NI treatment in the silt loam soil at 80% WHC. Likewise, the relative abundance of the studied nitrifying bacteria indicated that the NIs had only short-term effectiveness in the silt loam soil. These results suggested that DMPP and MP + TZ might trigger high N2O-N release from fine-textured soil with constant high moisture after this short-term inhibitory effect. In conclusion, DMPP and MP + TZ effectively reduce N2O-N emissions under soil drying and rewetting.

Effects of drying-rewetting cycles on colloidal phosphorus composition in paddy and vegetable soils

The impact of drying-rewetting (DRW) cycles on soil phosphorus (P) behavior is well-established; however, its impact on the different-sized colloidal P (CP) in agricultural soils is still unclear. To investigate the effect of DRW events on the mobilization of CP in agricultural soils, and to understand how this impact varies with different DRW cycles and drought intensities, the study explored the role of soil type, CP fractions, and compositions. The concentration of CP was measured in paddy soil and vegetable soil after 3, 6, and 9 DRW cycles of varying intensities. The CP was then fractionated into fine-sized colloids (FC-P; 1–220 nm), medium-sized colloids (MC-P; 220–450 nm), and coarse-sized colloids (CC-P; 450–1000 nm) through soil supernatant filtration. CP accounted for 71.1 % and 55.6 % of water-dispersible colloidal P (<1000 nm) in paddy and vegetable soils, with FC-P constituting the greatest proportion at 50 % and 44 % of CP respectively. The colloidal fraction correlated with organic carbon, aluminum, and iron. DRW cycles did not change the overall distribution of the three CP size fractions. However, they affected the concentration and composition of CP. This study concluded that DRW can have significant implications for nutrient release and water quality in agricultural soils and that maintaining soil moisture at 50 % to 70 % of water-holding capacity could alleviate CP accumulation resulting from DRW cycles.

Carbon dynamics as a function of soil moisture following repeated wet-dry cycles in irrigated soils

Drying-rewetting cycles are ubiquitous across natural and managed ecosystems. These cycles are known to mobilize carbon (C) in soils producing dramatic pulses in microbial respiration. While many factors contribute to these pulses, the drying-rewetting history of soils affecting carbon emissions remains unclear, especially in irrigated soils where soil moisture fluctuations are more repetitive and/or frequent than natural, seasonally influenced soils. To understand the controls of repeated wet-up and dry down effects on agricultural soils, we used a systems approach to examine the cross section of a furrow irrigated orchard to delineate soil C dynamics. Specifically, we compared two contrasting water regimes, (1) soils temporarily but repeatedly inundated during water delivery (i.e., furrows) and (2) soils at the base of trees (i.e., berms) that only receive water during precipitation events in a semi-arid Mediterranean climate. Overall, our findings show that the heterogeneous landscape of a furrow irrigated field results in two separate systems within the field scale in gaseous release of C as CO2, microbial selectivity of substrates, and mechanisms for C stabilization. By monitoring soil moisture as a function of depth for over two years, our results reveal that furrow soils undergo dramatic wet-dry cycles, while moisture within the berm is relatively constant. We were able to capture the distinct heterogeneity of soil moisture changes within the furrow and berm soils by continuously monitoring CO2 flux throughout water input events in both the wet and dry season. Soil CO2 efflux is suppressed upon irrigation within furrows, while carbon oxidation in berm soils exhibits pore-connectivity limitations that result in lower fluxes when dry. Solid phase soil C speciation determined by C 1s NEXAFS demonstrated C of higher aromaticity remained in furrow soil compared to berm soils. Microbial community analysis shows significantly different communities reside within berm and furrow soils, where furrow soils support more anaerobic metabolisms and spore-formers while berm soils have relatively higher abundance of aerobic microbes capable of degrading larger, more complex C compounds. Our findings show that water regime (periodic inundation vs episodic rainfall) controlling rewetting history can greatly differentiate C respiration within managed soils.

The effects of dunite fertilization on growth and elemental composition of barley and wheat differ with dunite grain size and rainfall regimes

Enhanced weathering (EW) of silicate rocks is a negative emission technology that captures CO2 from the atmosphere. Olivine (Mg2SiO4) is a fast weathering silicate mineral that can be used for EW and is abundant in dunite rock. In addition to CO2 sequestration, EW also has co-benefits in an agricultural context. Adding silicate minerals to soils can significantly improve crop health and growth as the weathering releases elements such as silicon (Si) that can stimulate crop growth and increase stress resistance, a co-benefit that is becoming increasingly important as global warming proceeds. However, dunite also contains heavy metals, especially nickel (Ni) and chromium (Cr), potentially limiting its use in an agricultural context. In this study, we investigate the influence of dunite addition on growth of barley and wheat in a mesocosm experiment. We amended the soil with the equivalent of 220 ton ha-1 dunite, using two grain sizes (p80 = 1020 µm and p80 = 43.5 µm), under two rainfall regimes (each receiving the same amount of 800 mm water y−1 but at daily versus weekly rainfall frequency). Our results indicate that the amendment of fine dunite increased leaf biomass but only with daily rainfall. Aboveground biomass was significantly reduced with weekly rainfall compared to daily rainfall, but this reduction was slightly alleviated by fine dunite application for wheat. This indicates a positive effect of dunite during drying-rewetting cycles. For barley the negative effect of reduced rainfall frequency was not counterbalanced by dunite application. Contrary to our expectations, calcium (Ca) and Si concentrations in crops decreased with fine dunite application, while, as expected, magnesium (Mg) concentration increased. Coarse dunite application did not significantly affect crop nutrient concentrations, most likely due to its lower weathering rate. In contrast to what was expected, plant Ni and Cr concentrations did not increase with dunite application. Hence, despite high dunite application in our experiment, plants did not accumulate these heavy metals, and only benefited from the released nutrients, albeit dependent on grain size and rainfall frequency.

Are carbon-storing soils more sensitive to climate change? A laboratory evaluation for agricultural temperate soils

A range of agroecological practices allow to increase soil organic carbon (SOC) stocks, which makes a positive impact on climate change mitigation and soil health, but the permanence of this additional SOC storage can be questioned, in particular in a climate change context. Increased temperatures, accentuated evaporation of terrestrial water and increased atmosphere moisture content are anticipated, resulting in more frequent droughts and heavy precipitation events. Understanding the SOC dynamics and assessing the sensitivity of carbon mineralization to these climatic events is necessary to anticipate future carbon losses in terrestrial ecosystems. To this respect, it seems relevant to investigate carbon-storing soils as increased carbon mineralization induced by climate change may limit the carbon storing potential in agricultural soils. Thus, we evaluated the sensitivity of SOC mineralization to increased temperature, decreased soil moisture and drying-rewetting cycles using soils from long-term field experiments. We performed an incubation experiment on topsoil (0–30 cm) samples from temperate luvisols that had been under 20 years under conservation agriculture (CA), organic agriculture (ORG) and conventional agriculture (CON-LC) at the La Cage experiment, and under organic waste products (OWPs) applications in QualiAgro experiment, including biowaste composts (BIOW), residual municipal solid waste composts (MSW), farmyard manure (FYM) and conventional agriculture without organic inputs (CON-QA). Soil samples were incubated in the lab for 3 months under different temperature conditions (20, 28 and 35 °C) or under different moisture conditions (matric potential: pF1.5; pF 2.5 and pF 4.2) or under several dry (pF 4.2)-wet (pF 1.5) cycles (DWC). The results shown that, whatever the agricultural practices, soil moisture regime and temperature significantly affect the SOC mineralization. Overall, the DWC did not stimulate soil carbon mineralization relative to wet controls (pF1.5 and pF2.5). Whatever the soil moisture regime and temperature, specific carbon mineralization was similar between agricultural practices at La Cage, while at QualiAgro, specific carbon mineralization was lower in soils receiving organic waste products (OWPs) compared to the baseline soil. These results suggest a strong carbon stabilization by OWPs in soils as assessed by laboratory incubation experiments. Within each long-term experiment, we observed no significant difference between the carbon-storing soils (CA, ORG, MSW, FYM and BIOW) and their respective baseline soils (CON-LC and CON-QA) in the delta SOC mineralized whatever the soil moisture regime. The Q10 also indicated no significant difference between carbon-storing soils and their respective baseline soils. These results indicate that the SOC mineralization in carbon-storing soils had a similar sensitivity to the soil moisture regime and temperature as the baseline ones. Hence, the implementation of these agroecological practices appears beneficial for climate change mitigation, even in the context of extreme climatic events.

Taxonomical and functional responses of microbial communities from forest soils of differing tree species diversity to drying-rewetting cycles

The predicted increases in drought in many forest ecosystems may alter soil microbial community diversity and activity, which may further depend on tree species richness. Shifts in microbial community composition and activity could engender changes in ecosystem function, notably, in soil greenhouse gas emissions and C storage. Using soils from mono-specific and mixed three-species forest stands from across Europe, we performed a microcosm experiment to test how soil microbial taxonomic and catabolic diversity are affected by repeated drying-rewetting (DRW) cycles and tree species mixing. We used Illumina sequencing and MicroResp™ analyses to explore community-level changes between microbial functional groups. DRW decreased bacterial richness and carbon substrate use diversity and increased fungal Shannon diversity. Additionally, microbial communities exposed to DRW changed their consumption of 11 out of 15 substrates significantly, suggesting microbial functional shifts. The legacy effect of tree species mixing influenced the structure of the microbial communities (i.e. taxonomic differential abundance) although, community weighted mean (CWM) values of absorptive root traits appeared to affect more strongly microbial richness, relative abundance, and Shannon diversity. No significant tree species mixing:DRW interaction was found for most microbial variables, except for the use of certain substrates and potentially differential abundance. Our data from a laboratory experiment with soils from different forest ecosystems underline that drought may cause shifts in microbial taxonomic and catabolic diversity, while tree species influences primarily taxonomic diversity through root traits.

Biocrusts regulate the effect of rainfall pulses on soil respiration at different temporal scales on the Loess Plateau

Rainfall pulses are one of the most important factors affecting soil respiration, especially in drylands where water is limited. Biocrusts are also crucial in dryland ecosystems and play important roles in regulating soil carbon cycling. However, it is unclear how biocrusts mediate the effects of rainfall pulses on soil respiration. Using continuous field monitoring, we explored whether and how different types of biocrusts (including cyanobacteria-, lichen- and moss-dominated biocrusts) modulate the effects of precipitation on soil respiration under drying-rewetting cycles at event and seasonal scales. We found that soil respiration was induced after rainfall pulses, especially at different types of biocrusted microsites. The increments of soil respiration during drying-rewetting cycles were greater in the moss-dominated biocrusts than in the bare soil, which suggested that the response of soil respiration to drying-rewetting cycles was increased by moss-dominated biocrusts. The length of the dry period, number of cycles, intensity of the dry period and frequency of rewetting affected the magnitude of soil respiration at event scales. However, when we analysed all of the drying-rewetting cycles together, that is, at the seasonal scale, the soil temperature was the driving factor affecting the maximum soil respiration after rainfall pulses. Therefore, soil temperature, rather than soil moisture, is the key factor regulating the response of soil respiration to drying-rewetting cycles at the seasonal scale. This study highlights the importance of temperature in regulating soil respiration under drying-rewetting cycles, and the regulatory factors of soil respiration vary at different temporal scales.

Moderate precipitation reduction enhances nitrogen cycling and soil nitrous oxide emissions in a semi-arid grassland.

The ongoing climate change is predicted to induce more weather extremes such as frequent drought and high-intensity precipitation events, causing more severe drying-rewetting cycles in soil. However, it remains largely unknown how these changes will affect soil nitrogen (N)-cycling microbes and the emissions of potent greenhouse gas nitrous oxide (N2 O). Utilizing a field precipitation manipulation in a semi-arid grassland on the Loess Plateau, we examined how precipitation reduction (ca. -30%) influenced soil N2 O and carbon dioxide (CO2 ) emissions in field, and in a complementary lab-incubation with simulated drying-rewetting cycles. Results obtained showed that precipitation reduction stimulated plant root turnover and N-cycling processes, enhancing soil N2 O and CO2 emissions in field, particularly after each rainfall event. Also, high-resolution isotopic analyses revealed that field soil N2 O emissions primarily originated from nitrification process. The incubation experiment further showed that in field soils under precipitation reduction, drying-rewetting stimulated N mineralization and ammonia-oxidizing bacteria in favor of genera Nitrosospira and Nitrosovibrio, increasing nitrification and N2 O emissions. These findings suggest that moderate precipitation reduction, accompanied with changes in drying-rewetting cycles under future precipitation scenarios, may enhance N cycling processes and soil N2 O emissions in semi-arid ecosystems, feeding positively back to the ongoing climate change.

Rapid remodeling of the soil lipidome in response to a drying-rewetting event

BackgroundMicrobiomes contribute to multiple ecosystem services by transforming organic matter in the soil. Extreme shifts in the environment, such as drying-rewetting cycles during drought, can impact the microbial metabolism of organic matter by altering microbial physiology and function. These physiological responses are mediated in part by lipids that are responsible for regulating interactions between cells and the environment. Despite this critical role in regulating the microbial response to stress, little is known about microbial lipids and metabolites in the soil or how they influence phenotypes that are expressed under drying-rewetting cycles. To address this knowledge gap, we conducted a soil incubation experiment to simulate soil drying during a summer drought of an arid grassland, then measured the response of the soil lipidome and metabolome during the first 3 h after wet-up.ResultsReduced nutrient access during soil drying incurred a replacement of membrane phospholipids, resulting in a diminished abundance of multiple phosphorus-rich membrane lipids. The hot and dry conditions increased the prevalence of sphingolipids and lipids containing long-chain polyunsaturated fatty acids, both of which are associated with heat and osmotic stress-mitigating properties in fungi. This novel finding suggests that lipids commonly present in eukaryotes such as fungi may play a significant role in supporting community resilience displayed by arid land soil microbiomes during drought. As early as 10 min after rewetting dry soil, distinct changes were observed in several lipids that had bacterial signatures including a rapid increase in the abundance of glycerophospholipids with saturated and short fatty acid chains, prototypical of bacterial membrane lipids. Polar metabolites including disaccharides, nucleic acids, organic acids, inositols, and amino acids also increased in abundance upon rewetting. This rapid metabolic reactivation and growth after rewetting coincided with an increase in the relative abundance of firmicutes, suggesting that members of this phylum were positively impacted by rewetting.ConclusionsOur study revealed specific changes in lipids and metabolites that are indicative of stress adaptation, substrate use, and cellular recovery during soil drying and subsequent rewetting. The drought-induced nutrient limitation was reflected in the lipidome and polar metabolome, both of which rapidly shifted (within hours) upon rewet. Reduced nutrient access in dry soil caused the replacement of glycerophospholipids with phosphorus-free lipids and impeded resource-expensive osmolyte accumulation. Elevated levels of ceramides and lipids with long-chain polyunsaturated fatty acids in dry soil suggest that lipids likely play an important role in the drought tolerance of microbial taxa capable of synthesizing these lipids. An increasing abundance of bacterial glycerophospholipids and triacylglycerols with fatty acids typical of bacteria and polar metabolites suggest a metabolic recovery in representative bacteria once the environmental conditions are conducive for growth. These results underscore the importance of the soil lipidome as a robust indicator of microbial community responses, especially at the short time scales of cell-environment reactions.A8GGwc64vbJf6mXPFnxxi9Video

High-resolution dynamics of available N in a grassland ecosystem under a multiple climate manipulation experiment

Nitrogen (N) is the most important limiting nutrient for plants and soil microorganisms in almost all ecosystems. Global environmental changes significantly affect the terrestrial N cycle but implications for plant available N in-situ remain unclear. Here, we investigated the simultaneous effect of elevated atmospheric CO2, an increase in temperature and a drying-rewetting event on diffusive N fluxes in soil of a managed permanent grassland at the multifactor climate manipulation experimental site ClimGrass (Austria) using microdialysis. Rewetting caused a significant, but short-lived increase in diffusive NH4+ and NO3− fluxes, which subsequently dropped until the end of the experiment. Harvesting induced a significant increase in diffusive NH4+ fluxes in the drying-rewetting treatments. However, elevated CO2 and soil warming had little effect on diffusive N fluxes. Our study suggests that more frequent soil drying-rewetting cycles associated with increased extreme weather events are uppermost among the climate change drivers affecting soil N availability.

The direct and legacy effects of drying‐rewetting cycles on active and relatively resistant soil carbon decomposition

AbstractGlobal climate change is expected to increase the frequency of drought and heavy precipitation, which could create more frequent drying‐rewetting cycles (DWC) in the soils. Although the DWC effects on soil organic carbon (SOC) decomposition have been widely studied, the effect of DWC and the subsequent legacy effect on the decomposition of different SOC pools is still unclear. We conducted a 128‐d laboratory incubation to investigate the DWC effects by using soils from old‐field for 15 years (OF, representing active SOC), bare‐fallow (BF)for 15 years, and BF for 23 years plus an extra 815‐d incubation (BF+, representing relatively resistant SOC). The experiment included nine 10‐d DWC of three treatments: (1) one mean constant‐moisture at 60% water‐holding capacity (WHC), (2) a mild DWC with 10‐d drying to 40% WHC and immediately rewetting to 80% WHC, and (3) a strong DWC with 10‐d drying to 20% WHC and immediately rewetting to 100% WHC. Following the DWC period (0–90 d), there was a 10‐d stabilization period (adjusting all treatments to 60% WHC), and then a 28‐d extended incubation under the constant moisture of 60% WHC. During the DWC period, the strong DWC had a strong effect on CO2 release compared with the constant‐moisture control, reducing the SOC decomposition of OF by 8% and BF by 10%, while increasing the SOC decomposition of BF+ by 16%. In addition, during the extended period, both mild and strong DWC significantly increased SOC mineralization of OF, but decreased that of BF and BF+. This legacy effect induced by DWC compensated for the changes in CO2 release during the DWC period, resulting in the minor response of SOC decomposition of OF and BF+ to the DWC during the entire incubation. Together, DWC could create both direct and legacy effects, and these effects vary with DWC intensity and SOC pools.

Effects of drying-rewetting cycles on the fluxes of soil greenhouse gases

Irregular precipitation caused by climate changes has resulted in frequent events of soil drying-rewetting cycles (DWC), which can strongly affect soil carbon (C) and nitrogen (N) cycling, including the fluxes of greenhouse gases (GHGs). The response of soil carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) fluxes to DWC events may differ among different ecosystem types and vary with experimental settings and soil properties, but these processes were not quantitatively assessed. Here, we evaluated the responses of soil GHG fluxes to DWC, compared with consistent moisture, as well as the associated driving factors with 424 paired observations collected from 47 publications of lab incubation experiments. Results showed that: (1) DWC significantly decreased soil CO2 emissions by an average of 9.7%, but did not affect the emissions and uptakes of soil CH4 and N2O; (2) DWC effects on soil GHG emissions varied significantly among different ecosystem types, with CO2 emissions significantly decreased by 6.8 and 16.3% in croplands and grasslands soils, respectively, and CH4 and N2O emissions significantly decreased and increased in wetlands and forests soils, respectively; (3) the effects of DWC on CO2 emissions were also positively regulated by organic C and N concentrations, pH, clay concentration, and soil depth, but negatively by C:N ratio and silt concentration, while DWC effects on N2O emissions were negatively controlled by C:N ratio, silt concentration, and soil depth. Overall, our results showed that CO2 emissions were significantly decreased by DWC, while the fluxes of CH4 and N2O were not affected, indicating an overall decrease of GHGs in response to DWC. Our results will be useful for a better understanding of global GHG emissions under future climate change scenario.

Dependence of cumulative CO2 emission and microbial diversity on the wetting intensity in drying-rewetting cycles in agriculture soil on the Loess Plateau

Altered drying-rewetting (DRW) procedures due to climate change may influence soil microbial properties and microbially-mediated carbon cycling in arid and semi-arid regions. However, the effects of DRW of different intensities on the microbial properties and respiration are not well understood. Thus, the responsive patterns of microbial communities and carbon mineralization in agriculture soil on the Chinese Loess Plateau to DRW treatments with different wetting intensities (5%–25% and 5%–36%) and frequency (1-cycle to 4-cycle) were investigated. Continuous moisture levels of 5%, 25% and 36% were used as control. Results revealed that the reduction of bacterial diversity and richness were greater for 5%–36% than 5%–25% treatment, while diversity of fungi was similar for different wetting intensities. Bacterial communities became clustered by wetting intensity rather than cycle number, however fungal community was unaffected by DRW. The complexity of bacterial co-occurrence network increased because of higher nodes, edges, average degree, diameter and average cluster coefficient after 4-cycles, and the interaction was more complex after 1-cycle for fungi. Rewetting caused a pulse-like increase of respiration rate, and the pulse amplitude was greater for DRW with high rewetting intensity and decreased with the increase of cycle number. The cumulative CO2 emission for DRW treatments was lower than that for the continuous moisture conditions. The net reduction of carbon release for 5%–36% treatment was 1.18 times higher than that for 5%–25% treatment. Our study provides experimental evidence of the positive potential of DRW processes for maintaining soil carbon stock in an agriculture system on the Loess Plateau.

Effect of Drying–Rewetting cycles on the metal adsorption and tolerance of natural biofilms

Drying–rewetting (D-RW) cycles can induce changes in biofilms by forcing the microbial community to tolerate and adapt to environmental pressure. Existing studies have mostly focused on the impact of D-RW cycles on the microbial community structure, and little attention has been paid to how D-RW cycles may change the biofilm tolerance and adsorption of heavy metals. We experimentally evaluated the effect of repeated D-RW cycles on the Cd2+ and Pb2+ adsorption and tolerance of biofilms. The equilibrium adsorption capacity of the biofilm decreased as the number of D-RW cycles was increased, which was attributed to a change in affinity between the biofilm and metal ions. For a binary metal system, the D-RW cycles affected the competitive adsorption of Cd2+ and Pb2+ by the biofilm. A synergistic effect was observed with one and three D-RW cycles, while an antagonistic effect was observed for the control film and five D-RW cycles. The tolerance of the biofilm to Cd2+ and Pb2+ increased with the number of D-RW cycles. The stress from the D-RW cycles may have increased the relative abundance of drought-tolerant bacteria, which altered the biofilm functions and thus indirectly affected the heavy metal adsorption capacity.