Drywood Termite Research Articles

The termite genus Glyptotermes (Isoptera: Kalotermitidae) in Miocene amber from Ethiopia

AbstractA new species of drywood termite (Isoptera: Kalotermitidae) is described from a nearly complete alate specimen preserved in early Miocene Ethiopian amber. Glyptotermes abyssinicus new species is distinguished by its U-shaped head with 12-segmented antennae, the ocelli separated from the eye margin, the right mandible with an obtuse angle between the apical and first marginal teeth, the left mandible with an obtuse angle between the apical and first + second marginal teeth, and the wing venation. This is the first termite reported from Ethiopian amber, and the fourth Miocene species of the extant genus Glyptotermes Froggatt, 1897, together with species previously described from diatomites of China and amber from the Dominican Republic. As the oldest report of the genus known from Africa, G. abyssinicus n. sp. constitutes an interesting new record for the biogeographical history of the kalotermitid lineage.UUID: http://zoobank.org/7670b045-fb31-4809-8116-4d14c4dd275b

Anti-termite activities of Indonesia’s essential oils against invasive drywood termite for wood product protection

The Indo-Malayan drywood termite, Cryptotermes cynocephalus (Light) (Kalotermitidae), is considered one of the most invasive drywood termites in the Southeast Asian region. Once infesting a timber, the colony of a drywood termite is very difficult to be controlled. Thus, the best way to mitigate a new infestation of a drywood termite is by applying chemical protection on wood. In recent years, particular attention has been given by researchers to develop and use organic termiticides, such as essential oils extracted from plants as active ingredients. The objective of the present study is to evaluate the repellency performance of several economically important essential oils from Indonesia, such as Clove (Eugenia caryophyllata), Cubeb Pepper (Piper cubeba L), and Lemon Grass (Cymbopogon winterianus Jowitt) against a drywood termite, C. cynocephalus. The test was performed by subjecting various concentrations of essential oil toward C. cynocephalus in the force-feeding test method. Sample weight-loss was evaluated after two weeks test. The results suggested that Clove oil has the highest repellence performance against C. cynocephalus, followed by Lemon Grass and Cubeb Pepper oils. The Termite mortality rate was also recorded to understand the toxicity performance of those essential oils.

The Resistance of Modified Manii Wood with Boric acid and Chitosan/Glycerol and Heating Against Fungi and Termites

Manii wood (Maesopsiss eminii Engl.) is a fast grown species that has low durability (class IV) and requires preservation. Boric acid is generally used in wood preservation. However, its application needs to be combined with other treatments to prevent from leaching. This study aimed to evaluate the resistance of manii wood after double impregnation of boric acid and chitosan or glycerol and heat treatment. Heating temperatures were 70ºC and 140ºC. The durability test was carried out against white rot fungus (Schizophyllum commune), dry wood termites (Cryptotermes cynocephalus), subterranean termites (Coptotermes curvignathus) according to SNI 7207-2014 standard, and field test based on ASTM D 1758-08 standard. The retention of boric acid in manii wood was 15,2 ± 1,0 kg ma−3. The interaction of boric acid and chitosan/glycerol impregnations and heat treatment significantly affected the wood resistance against decay fungi and termites. Impregnation of boric acid and glycerol by heating at 140ºC increased the resistance of wood against dry wood and subterranean termites. Double impregnation of boric acid and chitosan followed by heating at 140ºC was the best treatment in this research that improved significantly the resistance of manii wood against white rot fungi, dry wood termites and subterranean termites.

Distribution record of the invasive drywood termite Cryptotermes domesticus (Haviland) in Riau Province, Sumatra Island, Indonesia

The domestic drywood termite, Cryptotermes domesticus (Haviland), has been referred to in past literature as a native species originating in the Southeast Asia region. The species come to prominence due to its destructive damage to a dried wood log by creating passages and nests inside the wood. Its economic importance makes the species one of the monitored species in inter-island transport as part of a biosecurity initiative by the Indonesian Government. In the present study, we collected drywood termites’ specimens from four locations in Riau Province, Sumatra Island, and conducted morphological identification. In the end, we verify the presence of C. domesticus in all four sites studied. This finding establishes the continued presence of the species in Sumatra, one of the areas reported as the native distribution of the species other than Java and Kalimantan following the Holmgren reports in 1913. We believe this report can be an addition to the current status of C. domesticus in Sumatra and also be an addition to the biosecurity initiative list of monitored species regarding the C. domesticus native range in Indonesia.

Specific gravity, Extractive Content, and Natural Durability of Balsa (Ochroma pyramidale) Wood at 3 and 4 years old

The aim was to investigate the specific gravity, extractive content, and natural durability of balsa (Ochroma pyramidale) wood. A total of six trees of balsa at 3 and 4 years old were harvested and cut into the sample of specific gravity, extractive content, and natural durability test. Natural durability was tested according to SNI 01-7207-2006 against dry wood termites (Cryptotermes cynocephalus Light.). Specific gravity, extractive content, mass losses, and termite mortality were measured. Variance analysis was used to find the differences. The results showed that age influenced significantly on specific gravity and mass losses, while axial direction/position influenced significantly on extractive content and mass losses. Variance analysis also showed that radial direction influenced significantly on specific gravity and extractive content. The specific gravity of three years balsa wood is 0.14, which is lower than that of four years old three, which is 0,19. Extractive content in the bottom part (3.95%) is higher than that of in the middle (2.87%) and top (2.74%). Mortality in the bottom part (33.83%) is higher than that of in the middle (10.5%) and top part (5,8%) of the stem. In general, balsa wood is classified into durability class II-III.

Influence of jeringau (Acorus calamus Linn.) rhizome extract against dry-wood termites (Cryptotermes cynocephalus Light.)

Rubberwood (Hevea brasiliensis Muell Arg.) is classified as a hardwood, yet it is vulnerable to dry-wood termites attack, namely Cryptotermes cynocephalus Light. Hence, a preservative is necessary for prevention. The purpose of this research was to examine the effect of jeringau rhizome extract on dry-wood termites C. cynocephalus attack. The experimental design was a completely randomized factorial design with two factors include 2 levels of solvent (water and ethanol) and 4 levels of extract ratio (1:4, 1:6, 1:8, and 1:10). Parameters observed were actual retention, termites mortality, sample weight loss, and attack degree. Rubberwood was sized in 5 cm x 3 cm x 3 cm. The method used was immersing the samples in a container that contains jeringau rhizome extract in various treatments for 72 hours. The immersed samples were then fed to healthy and active C. cynocephalus for 12 weeks. The results showed that the type of solvents and extract ratio had a significant effect on termites mortality and samples weight loss, while actual retention was influenced by the extract ratio. In conclusion, the most effective formula to prevent dry-wood termites attack was jeringau rhizome extract with a weight ratio of jeringau powder and ethanol 1:6.

BIOACTIVITY ANALYSIS OF Annona glabra L. SEED EXTRACTS FOR WOOD PROTECTION AGAINST TERMITES (Cryptotermes cynocephalus Light. AND Coptotermes curvignathus Holmgren.)

Pond apple (Annona glabra L.) belongs to the family of Annonaceae. The seed of A. glabra contains bioactive substance that is toxic to some organisms, however the effectiveness to control wood degrading termites has not yet been scientifically reported. This research analyzes the efficacy of A. glabra seed extract to wood degrading termites. Seed extraction was conducted using n-hexane and ethyl acetate. The paper disc test showed that the extract of A. glabra is toxic to Cryptotermes cynocephalus (dry-wood termites) and Coptotermes curvignathus (subterranean termites). The higher extract concentration (up to 63%) resulted in a higher termite mortality (up to 100%) and lower weight loss of paper sample (less than 1%). Ethyl acetate extract of A. glabra seeds has a better toxicity effect than n-hexane extract against dry wood termites and subterranean termites.

Physical-mechanical properties and durability enhancement of glued laminated lumber made from inner part of Gewang (Corypha utan Lam.) trunk: The effect of lamina densification and lumber smoking

The inner part of gewang (Corypha utan Lam.) trunk has significantly lower physical-mechanical properties as well as biodeterioration resistance, compared to outer parts. This study investigated a method for improving the physical-mechanical properties and biodeterioration resistance of glued laminated lumber made from the inner part of gewang trunk (GLIT). The effects of pretreating the lamina with densification and exposing the GLIT to smoking process were investigated. The seven layers of GLIT samples were bonded with isocyanate adhesive at a glue spread of 280 g/m2, then smoked for 15 days. Solid lumber pieces made from the inner part as well as the outer part of GLIT were also prepared for comparative purposes. Physical and mechanical properties of the lumber samples were tested according to Japanese agricultural standards. Meanwhile, dry-wood termite and wood decaying fungi resistances of the lumber samples were determined according to Indonesian standard. Results showed that overall physical-mechanical properties as well as dry-wood termite and decay resistances of densified-smoked GLIT were enhanced significantly. Pretreating of lamina and post-production smoking can be considered as a potential method to improve physical-mechanical properties and biodeterioration resistance of the GLIT.

The use of Annona squamosa seed, leaf and bark as an alternative in the control of Cryptotermes brevis

The changes in human behavior towards the environment, the disorderly urban development, globalization and climate change are some of the factors that promote the occurrence and spread of pests that somehow bring damage. This occurrence is aimed at termites, which are insects known for their habit of feeding on cellulose, that is, papers, books, and wooden structures, causing accidents. Cryptotermes brevis are a major cause of damage to various wood species commonly used throughout the country, which cause major public health problems. Annonaceae have been extensively investigated because they present many species as a source of insecticidal compounds with different modes of action on insects. Therefore, this work aims to develop a natural, economical and sustainable product by using the extracts of the seeds of the fruit of the earl (Annona squamosa) as a resource to combat dry wood termites. From the results, it was possible to prove the veracity of the hypothesis that the natural insecticides developed from the extract of the seeds of the fruit of the earl (Annona squamosa) are very efficient, and are effective in the issue of mortality rates of Cryptotermes brevis.

Termite Pest Identification Method Based on Deep Convolution Neural Networks.

Several species of drywood termites, subterranean termites, and fungus-growing termites cause extensive economic losses annually worldwide. Because no universal method is available for controlling all termites, correct species identification is crucial for termite management. Despite deep neural network technologies' promising performance in pest recognition, a method for automatic termite recognition remains lacking. To develop an automated deep learning classifier for termite image recognition suitable for mobile applications, we used smartphones to acquire 18,000 original images each of four termite pest species: Kalotermitidae: Cryptotermes domesticus (Haviland); Rhinotermitidae: Coptotermes formosanus Shiraki and Reticulitermes flaviceps (Oshima); and Termitidae: Odontotermes formosanus (Shiraki). Each original image included multiple individuals, and we applied five image segmentation techniques for capturing individual termites. We used 24,000 individual-termite images (4 species × 2 castes × 3 groups × 1,000 images) for model development and testing. We implemented a termite classification system by using a deep learning-based model, MobileNetV2. Our models achieved high accuracy scores of 0.947, 0.946, and 0.929 for identifying soldiers, workers, and both castes, respectively, which is not significantly different from human expert performance. We further applied image augmentation techniques, including geometrical transformations and intensity transformations, to individual-termite images. The results revealed that the same classification accuracy can be achieved by using 1,000 augmented images derived from only 200 individual-termite images, thus facilitating further model development on the basis of many fewer original images. Our image-based identification system can enable the selection of termite control tools for pest management professionals or homeowners.

Tomato tolerance and purple nutsedge control with sulfuryl fluoride mixes

AbstractSulfuryl fluoride (SF) is currently used as a fumigant for control of drywood termites and insects in building structures, vehicles, wood products, postharvest commodities, and food processing facilities. This research investigated the feasibility of using SF as a preplant soil fumigant for purple nutsedge control in plastic-mulched tomato production. SF treatments included SF injected through drip tapes or SF injected through drip tapes a few hours following shank injection of chloropicrin (Pic). Results revealed that SF alone at 224, 336, or 448 kg ha−1 was generally less effective compared with when it was applied in conjunction with Pic at 168 kg ha−1. SF alone provided inconsistent control of purple nutsedge. In contrast, SF + Pic was as efficacious or more efficacious on purple nutsedge than the industry standards, including 1,3-dichloropropene (1,3-D) plus Pic and metam potassium. None of the fumigant treatments visually injured tomato plants, stunted growth, or adversely affected tomato yield. In one of the four tomato seasons, tomato plants growing in plots fumigated with SF + Pic resulted in taller tomato plants and higher markable yields. Results indicate that soil fumigation with SF + Pic is safe on plastic-mulched tomato and effectively controls purple nutsedge.

Pathogenicity of entomopatogenic fungi isolates from infected pest crop against dry wood termites Cryptotermes sp. (Ioptera: Kalotermitidae)

Through exploration at several farming regions in X Koto sub distric, Tanah Datar regency, West Sumatera province (Kandang Sampia, Kayu Tanduak, Panyalaian, Koto Baru), were found 38 fungi isolates. Some isolates (31.58%) pathogenic against drywood termites, Cryptotermes sp. Most of those fungi belong to Ascomycota division (Aspergillus niger, Aspergillus sp. 1, Aspergillus sp. 2, Aspergillus sp. 3, Aspergillus sp. 4, Aspergillus sp. 5, Penicillium sp. 1, Penicillium sp. 2, Penicillium sp. 3, Penicillium sp. 4), while 2 fungi did not identified yet (Sp. 1 and Sp. 2). A. niger is the most effective fungi to control Cryptotermes sp., that indicated by its ability to kill termites (at 107 conidial/ml could kill 93.94% after 7 days infected, higher viability and spore formation), but not differed with Aspergilus sp. 1 and Penicillium sp. 2. The lowest ability showed by Sp. 1 fungi (at 107 conidial/ml only could kill 60.61% after 7 days infected).

The Durability of Teak Sapwood from Community Forest Treatened by Boron Compound though Lowry-Preservation Method against Subterranean And Dry-Wood Termites

Nowadays, most of teak wood that available on the market was mainly from comunity forest. Those teak woods were commonly harvested in the early age. The young teak wood from community forests possesses lower proportion of heartwood than sapwood. Those condition was generally had an affect on the wood durability. Although the wood durability was estimated to be low, but the teak wood from community forest was intensively used for furniture and house construction. Therefore, study on the sapwood durability of young teak wood from community forest is necessary. In the present study, the durability of sapwood treated by boron compound (boric acid and borax) as wood preservatives was investigated. The boron concentrations were 5, 7 and 10%. The vacuum process were used to impreg the preservative into the wood specimens called as Lowry method. The efectivity of preservation method were investigated, namely absorption, retention, penetration, efficacy of subterranean and dry-wood termites. The absorption, retention, and penetration of boron compound were 69.10–96.41 kg/m3, 4.53–5.31 kg/m3, and 3.04–3.16 mm, respectively. Absorption, retention, and penetration of preservatives showed an increasing value by increasing the preservatives concentrations, with the highest values were obtained for 10% boron concentration. Graveyard test was used to evaluate the efficacy of boron in teak sapwood to subterranean termites. During 2 months observation, mass loss and degree of wood damage were 0.42-1.37% and 6.31-18.72%. Furthermore, the efficacy of boron was also conducted for dry-wood termites. The mass loss, degree of wood damage, and dry-wood termites mortality after 28 days observation were 1.46-1.67%, 29.45-32.38%, and 87.33-95.33%, respectively. The durability of boron treatened teak sapwood against subterranean and dry-wood termites was increased. The increasing of teak sapwood durability was characterized by reduction of mass loss and degree of wood damage compared to untreated teak sapwood (control).

Searching for biological feedstock material: 3D printing of wood particles from house borer and drywood termite frass.

Frass (fine powdery refuse or fragile perforated wood produced by the activity of boring insects) of larvae of the European house borer (EHB) and of drywood termites was tested as a natural and novel feedstock for 3D-printing of wood-based materials. Small particles produced by the drywood termite Incisitermes marginipennis and the EHB Hylotrupes bajulus during feeding in construction timber, were used. Frass is a powdery material of particularly consistent quality that is essentially biologically processed wood mixed with debris of wood and faeces. The filigree-like particles flow easily permitting the build-up of wood-based structures in a layer wise fashion using the Binder Jetting printing process. The quality of powders produced by different insect species was compared along with the processing steps and properties of the printed parts. Drywood termite frass with a Hausner Ratio HR = 1.1 with ρBulk = 0.67 g/cm3 and ρTap = 0.74 g/cm3 was perfectly suited to deposition of uniformly packed layers in 3D printing. We suggest that a variety of naturally available feedstocks could be used in environmentally responsible approaches to scientific material sciences/additive manufacturing.

Do chewing cues from drywood termites mediate recruiting for wood colonization?

AbstractDrywood termites, Cryptotermes brevis (Walker) (Isoptera: Kalotermitidae), are eusocial insects living and foraging in groups inside pieces of wood, in which they perform a suite of behavioral traits ranging from individual to coordinated group activities. Vibroacoustic cues play important roles in behavioral mediation during defense and foraging activities. However, termite societies also exhibit other relevant behaviors, including substrate selection for colony establishment, which has received little attention. Therefore, we tested the hypothesis that vibrational cues produced by termites inside pieces of drywood influence wood recruiting of conspecifics using dichotomous choice tests. In addition, we assessed whether the choice affects colony success (i.e., termite survival and wood consumption). Two behavioral activities were recognized among drywood termites colonizing wood galleries – walking and chewing. However, only the chewing cues overcome background noise, allowing acoustic signal characterization and playback tests; thus, they were the focus of our study. Overall, drywood termite groups exhibit random choice among wood‐testing unities, indicating that the chewing cues from the natural and/or playback signals are not used as a social trigger to mediate conspecific recruiting for wood preference and colonization. Nonetheless, the establishment of termite colonies in infested vs. non‐infested wood pieces led to better termite performance in the former, exhibiting higher wood consumption and survival.

Resistance of particleboards produced with ligno-cellulosic agro-industrial wastes to fungi and termites

This study aimed to evaluate the resistance of medium density particleboard (MDP) made with ligno-cellulosic agro-industrial wastes to Brunneoporus malicola (≈Gleophyllum trabeum) and Trametes versicolor fungi and Cryptotermes brevis and Nasutitermes corniger termites. The panels were produced by compression, with different proportions of eucalypt sawdust, macadamia nut carpel, papaya stalk and coffee husks (10%, 20% and 30% each one and the control - 100% eucalypt sawdust) and bonded with urea-formaldehyde (UF) and tannin-urea-formaldehyde (TUF). The fungi and termite tests were performed in conformity the specific standards of wood and wood-based materials biological resistance. The resistance of each treatment was evaluated in terms of the composite mass loss and mortality for termite. All treatments submitted to the attack of Brunneoporus malicola and Tramites versicolor fungi were classified as moderately resistant or no resistant, no statistical difference. Highlight for 30% macadamia nut carpel with less mass loss, 42.28% and 43.58% in the UF and TUF panels, respectively. There were no statistical differences for mass loss and mortality between treatments and adhesives to dry-wood termites; treatment with 10% and 30% macadamia nut carpel showed greater mass loss (0.681 g) and mortality (56.50%), respectively. In general, all treatments showed low resistance to fungi and for dry-wood termite attack good resistance, indicating these case biological properties of the panels. The panels produced did not allow resistance to attack of Nasutitermes corniger termite.

MONITORING THE TOXICITY OF DRY WOOD TERMITES USING THREE PLANT POWDERS IN LABORATORY CULTURE

In order to model a suitable control alternative for subterranean termites and termites infesting wooden furniture and cabinets where consumables are likely stored, this present study monitored the efficacy of three plant powders at different doses (0.50-1.50g) against the dry wood termite,Cryptotermes cavifrons Banks (Blattodea). Plant materials were locally prepared into powders and assigned to 20 wood termites in 5g substrates of sand and stick. The culture was set up in triplicates and lethal concentrations were determined by Probit analysis. The plant powder could not exerted 100% mortality, but the highest mortality was recorded in Dennettia tripetala (1.50g) on stick (mean= 8.33±0.80). All concentration of D. tripetala on sand and stick caused mortality within 0 to 48 hours after exposure and compared favorably with Permethrin which recorded the highest mean time mortality on sand at 0-24 hrs after exposure. The lowest LC50value (0.75 and 0.79g/g) corresponded to Permethrin and D. tripetala on sand and stick respectively. This results suggest that best concentrations of pepper fruit powder can be adopted as potential alternative to chemical powders.

Linear Expansion and Durability of a Composite Boards (MDF Laminated Using Three Selected Wood Veneers) against Drywood Termites

This research was conducted to investigate the linear expansion and resistance properties of a composite board (com-ply). This board was made of medium-density fiberboard (MDF) laminated using avocado (Persea americana), mahogany (Swietenia mahogani), and pine (Pinus merkusii) veneers. These three types of veneers were laminated on both surfaces of the MDF using adhesives, namely, epoxy and isocyanate. Glue (250 g·m−2) was spread on the surface, followed by cold press for 3 h with an applied pressure of 15 kg·cm−2. The research result revealed that com-ply exhibited an increased dimensional stability compared with MDF, indicated by reduction in water absorption, thickness swelling, and linear expansion. The com-ply made of the pine veneer and isocyanate adhesive exhibited high density, water absorption, thickness swelling, and screw withdrawal load. The com-ply that exhibited the strongest resistance to drywood termite attacks was the one made of the mahogany veneer and isocyanate adhesive. Moreover, the com-ply that exhibited the biggest weight loss (3.6 %) was made of the pine veneer and epoxy adhesive. The results of this research may facilitate in manufacturing com-ply using the selected veneer and adhesive without the application of hot press.

Evaluation of Color Change and Biodeterioration Resistance of Gewang (Corypha utan Lamk.) Wood

Gewang (Corypha utan Lamk.) is one of the endemic palm species which has been used as a building material for many years in Indonesia. The objective of this study was to enhance the overall resistance of gewang wood to biological deterioration by using smoke treatment. Samples taken from different parts of the trunks, namely bottom, middle, and upper parts in a longitudinal direction and outer and inner parts in a transversal direction, were exposed to the smoking process. Discoloration, dry-wood termite (Cryptotemes cynocephalus) and fungi (Schizophyllumcommune) resistance of smoked samples were determined according to the Indonesian standard. Based on the findings in this work, discoloration of smoked specimens was more prominent than that of the unsmoked samples. Overall termite and decay resistance of smoked samples were enhanced and higher than those of unsmoked samples without any influence of longitudinal and transversal orientations within the trunks. It appears that smoking can be considered as a potential method to improve decay and termite resistances of gewang wood.

Volatile Essential Oils Can Be Used to Improve the Efficacy of Heat Treatments Targeting the Western Drywood Termite: Evidence from Simulated Whole House Heat Treatment Trials.

Colonies of western drywood termites, Incisitermes minor (Hagen) (Blattodea: Kalotermitidae), are difficult to detect and treat due to their cryptic nature. The use of heated air to create lethal temperatures within infested wood serves as a nonchemical treatment option targeting whole structure or large portions of the structure. However, the presence of hard-to-heat areas and potential risk of damage for heat-sensitive items are recognized as important challenges. Here, we tested if a localized injection of volatile essential oil could be utilized to address the heat sink issue, potentially increasing the overall efficiency of heat treatments against drywood termites. Artificially infested wooden blocks were placed in several locations of the test building, and heat treatments were conducted. For the treatment group, a small amount of essential oil (methyl salicylate) was added in the blocks prior to the heat treatment. All blocks placed in uninsulated wall voids had 92-100% termite mortality by day 7. However, the presence of a large concrete wall in the subarea hindered heating of blocks therein, resulting 36-44% mortality by day 7 when there was no essential oil treatment. Incorporation of the essential oil substantially increased the control efficacy for the subarea, resulting in more than 90% mortality. This approach might also be helpful in reducing the risk of potential heat damage during heat treatment without compromising its control efficacy.