Dry Root Rot Of Chickpea Research Articles

In-vivo evaluation of different fungicides, plant extracts, bio-control agents and organics amendments for management of dry root rot of chickpea caused by Macrophomina phaseolina

The present study was planned to evaluate the efficacy of various fungicides, plant extracts, biocontrol agents and oil cakes against Macrophomina phaseolina causing dry root rot of chickpea. Among the tested biocontrol agents against Macrophomina phaseolina, T. viride was found the most effective against the fungus followed by T. harzianum, Bacillus subtalis and P. fluorescens was the least effective in reducing root rot incidence. Among the fungicides carbendazim was found most effective and recorded minimum root rot incidence. Among plant extracts, garlic extract was found most effective in reducing root rot incidence followed by neem leaf extract. In the case of organic amendments, Neem cake was the most effective in reducing the root rot incidence while wool waste and goat manure was found least effective in controlling root rot incidence.

Biocontrol potential of Trichoderma harzianum as seed bio-priming against dry root rot of chickpea

Biocontrol potential of <i>Trichoderma harzianum</i> as seed bio-priming against dry root rot of chickpea

Variation in virulence of Macrophomina phaseolina isolates causing dry root rot of chickpea and performance of chickpea genotypes against this disease

Twenty isolates of Macrophomina phaseolina were collected from ten major chickpea growing states of India. Virulence of the isolates were tested on susceptible chickpea cultivars viz., H 09-23, L-550 and BG-212. A total of 215 chickpea genotypes were screened in sick plot, none of them were found resistant. However, only two entries viz., RSG 888 (2) and ICCV 93706 showed moderately resistant reaction.

Biocontrol Potential of Drought tolerant Rhizospheric bacteria Isolated from Stressed Ecosystems against Dry Root Rot of Chickpea (Cicer arietinum L.) Caused by Rhizoctonia bataticola

Biocontrol Potential of Drought tolerant Rhizospheric bacteria Isolated from Stressed Ecosystems against Dry Root Rot of Chickpea (<i>Cicer arietinum</i> L.) Caused by <i>Rhizoctonia bataticola</i>

Evaluation of bio-control agents for management of dry root rot of chickpea caused by Macrophomina phaseolina

Dry root rot of chickpea caused by Macrophomina phaseolina is an important disease affecting chickpea production areas in India, the largest producer of this crop in the world. The disease is primarily controlled through the use of fungicidal seed treatments. In this study, several isolates of two bio-control agents, Trichoderma viride and Pseudomonas fluorescens, were assessed for their ability to reduce the growth of M. phaseolina under laboratory conditions and subsequently used for field studies. The most effective isolate of each bio-control agent and the commonly used chemical seed-treatments, carbendazim and hexaconazole were evaluated. In addition to the use of the fungicides and bio-control agents individually as seed treatments, the study also included a trial combining soil application through bio-agent enriched farm-yard manure, along with seed treated with the bio-control agent. It was found that this combination showed maximum germination, least root rot incidence and highest yields as compared to the other biological or chemical seed treatments included in this study. This paper reports the identification of native isolates of T. viride and P. fluorescens that can be used under field conditions to control dry root rot of chickpea in tropical regions and demonstrates the use of a method of application that could significantly improve the efficacy of disease control while using bio-control agents.

Evaluation of seed dressing and soil application formulations of Trichoderma species for integrated management of dry root rot of chickpea

The efficacy of the newly developed seed dressing and soil application formulations of Trichoderma viride, T. virens and T. harzianum were evaluated individually and in combinations under pot and field experiments for the management of dry root rot (Rhizoctonia bataticola) of chickpea (Cicer arientinum). In pot experiments, T. harzianum based seed dressing formulation, Pusa 5SD, and soil application formulations, Pusa Biogranule 5 (PBG 5) and Pusa Biopellet 10G (PBP 10G), were found to be effective in reducing dry root rot incidence in chickpea and increasing the seed germination, shoot and root lengths of the crop. Under field experiments, a combination of soil application of T. harzianum based PBP 10G and seed treatment with Pusa 5SD+carboxin was found to be the best by providing the highest seed germination, shoot and root lengths and grain yield and the lowest dry root rot incidence in chickpea.

Determination of genetic diversity among Indian isolates of Rhizoctonia bataticola causing dry root rot of chickpea

Genetic diversity among 27 isolates (23 from chickpea and 4 from other host crops) of Rhizoctonia bataticola representing 11 different states of India was determined by random amplified polymorphic DNA (RAPD), internal transcribed spacer restriction fragment length polymorphism (ITSRFLP) and ITS sequencing. The isolates showed variability in virulence test. Unweighted paired group method with arithmetic average cluster analysis was used to group the isolates into distinct clusters. The clusters generated by RAPD grouped all the isolates into six categories at 40% genetic similarity. High level of diversity was observed among the isolates of different as well as same state. Some of the RAPD (OPN 4, OPN 12, and OPN 20) markers clearly distinguished majority of the isolates into the area specific groups. The ITS I, 5.8 rDNA and ITS II regions of 11 isolates representing different RAPD groups were amplified with primers ITS 1 and ITS 4 and digested with seven restriction enzymes. The restriction enzymes DraI, MboI, RsaI, and AluI were found to be suitable for differentiating the isolates into five categories by showing isolate specific ITS-RFLP patterns. The isolates were variable in their nucleotide sequences of the ITS regions. This is the first study on genetic diversity among chickpea isolates of R. bataticola.

Inheritance of Dry Root Rot (Rhizoctonia bataticola) Resistance in Chickpea (Cicer arietinum)

AbstractThe inheritance of resistance to dry root rot of chickpea caused by Rhizoctonia bataticola was studied. Parental F1 and F2 populations of two resistant and two susceptible parents, along with 49 F1 progenies of one of the resistant × susceptible crosses were rested for their reaction to dry root rot using the blotting‐paper technique. All F, plants of the resistant × susceptible crosses were resistant; the F2 generation fitted a 3 resistant: 1 susceptible ratio indicating monogenic inheritance, with resistance dominant over susceptibility. F3 family segregation data confirmed the results. No segregation occurred among the progeny of resistant × resistant and susceptible × susceptible crosses.