Soil tillage intensity can be expected to affect the greenhouse gas balance of arable cropping systems through effects on soil physics and biology affecting soil carbon storage and nitrous oxide (N 2O) emissions. The effects of conventional tillage (CT) with ploughing to 20 cm, reduced tillage (RT) with harrowing to 8–10 cm and direct drilling (DD) on CO 2 and N 2O emissions from a loamy sand soil (8.1% clay, 3.5% organic matter) under spring barley undersown with ryegrass were measured by static chambers over a period of 113 days in spring and summer 2004 in a tillage experiment established in 2002 at Foulum, Denmark. There was a high temporal and spatial variation in both CO 2 and N 2O emissions, which made the comparisons of treatment effects on emissions on single dates difficult. However, this variation was reduced when the measurements were corrected for diurnal variation in the emissions and when emissions were cumulated over a longer period. Both CO 2 and N 2O emissions decreased in the order CT > RT > DD. Compared with CT (40 kg C day −1) the cumulated CO 2 emissions during the 91 days after tillage were 21 and 25% lower for the RT and the DD treatments, respectively. The cumulated N 2O emission from CT over the entire observation period (0.89 kg N ha −1 or 7.9 g N day −1) was about twice that of DD. The N 2O emissions were significantly higher for CT compared with DD and RT, even before tillage and the difference increased after tillage, but decreased after fertilisation. Spring barley dry matter grain yields were reduced by 14% for RT and 27% for DD compared to CT. Measurements of soil mineral nitrogen (N) at sowing showed no difference between the treatments, and could thus not explain the differences in N 2O emissions and crop N uptake. It is likely that tillage affected CO 2 emissions, N 2O emissions and crop growth through different processes, where effects of soil compactness on root penetration and soil aeration and diffusivity on one side and soil organic matter turnover on the other side probably played key roles.