The progressive study on oat (Avena sativa L.) transpired during the crop season of 2021–2022 at the Agricultural Research Station, College of Agriculture, University of Basrah, Iraq. The study aimed to determine the effects of amino acids foliar application on two oat cultivars. Experimenting with a randomized complete block design included a split-plot arrangement. Two oat cultivars (Shafa and Genzania) grown and placed in secondary plates received seven treatments of three different amino acids (Control – No amino acid, 50 and 100 mg L-1 of L-Tryptophan, 50 and 100 mg L-1 of L-Glycine, and 50 and 100 mg L-1 of L-Lysine). The results revealed that oat cultivars and amino acid treatments differed significantly for most of the studied traits. The amino acid foliar application treatment of LTryptophan at the rate of 50 mg L-1 showed significant superiority, which boosted and provided the highest rate of flag leaf area, chlorophyll content, crop growth rate, and green fodder yield. However, the cultivar Shafa exhibited superiority for flag leaf area, chlorophyll content, and the green and dry fodder yield, with increased values of 7.15 cm2, 8.11 µg cm-3, and 8.01% and 5.61% t ha-1, respectively.